Resource data sheet
Prev.
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pcDNA3/hArf6(Q67L)-HA

Expression vector of human ARF6, dominant activating mutant Q67L.

Catalog number RDB15350
Resource name pcDNA3/hArf6(Q67L)-HA
Alternative name W54
Clone info. Expression vector of human ADP-ribosylation factor6 (ARF6), dominant activating mutant Q67L, tagged with HA epitope at C-ter.
Comment Expression was confirmed by the depositor with immunostaining.
Vector backbone pcDNA3 (plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cels).
Gene/insert name human ARF6 cDNA
Depositor|Developer Nakayama, Kazuhisa |
Other clones in our bank

External Database
human ARF6

          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Makyio, H. et al. EMBO. J., 31 (11): 2590-2603, 2012). RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization.
          Ordering Please visit Information of Request for Distribution.[link] 
          Order form 
          [Credit Card Payment]  [Bank Transfer Payment]
          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Makyio, H. et al. EMBO. J., 31 (11): 2590-2603, 2012)。本件リソースは非営利機関にのみ提供し、利用範囲は学術研究に制限する。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB15350 pcDNA3/hArf6(Q67L)-HA DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The pcDNA3/hArf6(Q67L)-HA was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15350).

          Reference section:

          Makyio, H., Ohgi, M., Takei, T., Takahashi, S., Takatsu, H., Katoh, Y., Hanai, A., Ueda, T., Kanaho, Y., Xie, Y., Shin, H.W., Kamikubo, H., Kataoka, M., Kawasaki, M., Kato, R., Wakatsuki, S., Nakayama, K., Structural basis for Arf6-MKLP1 complex formation on the Flemming body responsible for cytokinesis. EMBO J. 31 (11): 2590-2603 (2012). PMID 22522702.

          Further references such as user reports and related articles (go to bottom)


          References and tips

          Featured content


          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB15350_A7I5p1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV_Forward (Pr0016)
          Region: CMV pro, insert (hArf6 (Q67L)), HA
          Sequence file: RDB15350_A7I5a.seq check
          >D03588B5_A7I5_1_CMV_Forward_C03_09_ABI24.ab1
              1 NNNNNNNNNN NNNNNTNNNN GCNNTGGGCG GTANGCGTGT ACGGTGGGAN GTCTATATAA
             61 GCAGAGCTCT CTGGCTAACT AGAGAACCCA CTGCTTACTG GCTTATCGAA ATTAATACGA
            121 CTCACTATAG GGAGACCCAA GCTTGGTACC GAGCTCGGAT CTGCCACCAT GGGGAAGGTG
            181 CTATCCAAAA TCTTCGGGAA CAAGGAAATG CGGATCCTCA TGTTGGGCCT GGACGCGGCC
            241 GGCAAGACAA CAATCCTGTA CAAGTTGAAG CTGGGCCAGT CGGTGACCAC CATTCCCACT
            301 GTGGGTTTCA ACGTGGAGAC GGTGACTTAC AAAAATGTCA AGTTCAACGT ATGGGATGTG
            361 GGCGGCCTGG ACAAGATCCG GCCGCTCTGG CGGCATTACT ACACTGGGAC CCAAGGTCTC
            421 ATCTTCGTAG TGGACTGCGC CGACCGCGAC CGCATCGATG AGGCTCGCCA GGAGCTGCAC
            481 CGCATTATCA ATGACCGGGA GATGAGGGAC GCCATAATCC TCATCTTCGC CAACAAGCAG
            541 GACCTGCCCG ATGCCATGAA ACCCCACGAG ATCCAGGAGA AACTGGGCCT GACCCGGATT
            601 CGGGACAGGA ACTGGTATGT GCAGCCCTCC TGTGCCACCT CAGGGGACGG ACTCTATGAG
            661 GGGCTCACAT GGTTAACCTC TAACTACAAA TCTCTCGAGT ATCCATACGA TGTACCAGAC
            721 TACGCAGAGN TCCACTAGTT CTAGAGGGCC CTATTCTATA GTGTCACCTA AATGCTAGAG
            781 CTCGCTGATC AGCCTCNNCT GTGCCTTCTA GTTGCCAGCC ATCTGTTGNT TGCCCCTCCC
            841 CCCNNGCCTT CCTTGACCCT GNAANGTGCC ACTCCNACNG NCCTTTCCNN ANNAAANGAN
            901 GNAANTGCAT CGCNNNNNCT NANNNNGNNN CNNTCTATNC TGGGGGGGGN NGGGNNNGGN
          961 NNNNNNNNN
          //
          Primer: SV40pro_F (Pr0110)
          Region: NeoR
          Sequence file: RDB15350_A7I5b.seq check
          >D03588B5_1_A7I5_SV40pro_F_H03_24_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNAN NNGTGNNGNN GNTTTTTTGG AGGCCTAGGC TTTTGCAAAA
             61 AGCTAACTTG TTTATTGCAG CTTATAATGG TTACAAATAA AGCAATAGCA TCACAAATTT
            121 CACAAATAAA GCATTTTTTT CACTGCATTC TAGTTGTGGT TTGTCCAAAC TCATCAATGT
            181 ATCTTATCAT GTCTGGATCC GCTGCATTAA TGAATCGGCC AACGCGCGGG GAGAGGCGGT
            241 TTGCGTATTG GGCGCTCTTC CGCTTCCTCG CTCACTGACT CGCTGCGCTC GGTCGTTCGG
            301 CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG
            361 GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG
            421 GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA
            481 CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT
            541 GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC
            601 TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTANGTA TCTCAGTTCG
            661 GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC
            721 TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA
            781 CTGGCAGCAG CCACTGGTAA CAGGNNTAGC AGANCGAGGT ATGTANGCGG TGCTACAGAN
            841 TTCTTGAAGT GGTGGCCNNA CTACNGCTAC ACTAGAAGAA CAGTATTNGG TATCTGCGCN
            901 CTGCTGAAGC NNGTNANCTT CGGAAAAAGN NTTGGTANCT NNNGNTCCNG CAAANNAACC
          961 NNNGCNGGNA NCNGNNNGNT TTTTTNNNTN NNNNNCNNNN NNNNNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Makyio, H., Structural basis for Arf6-MKLP1 complex formation on the Flemming body responsible for cytokinesis. EMBO J. 31 (11): 2590-2603 (2012). PMID 22522702.

          2023.05.01

          GNP_filter3_RDBDEP_html_230501.pl