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pGEX-4T-2/hGGA3(GAT) (#RDB15367)

Bacterial Expression vector of human GGA3, the GGA homology domain.


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Publication Takatsu, H., Biochem. J. 365 (Pt 2): 369-378 (2002). PMID 11950392. [PubMed] [Article] [RRC of NBRP]
Test sheet Data Sheet open 
 
Clone info. Bacterial Expression vector of human golgi-associated, gamma adaptin ear containing, ARF 3 (GGA3), the GGA homology domain (107-342 amino acid residues), tagged with glutathione S-transferase (GST).
Comment Expression was confirmed by the depositor with western blotting
Vector backbone pGEX-4T-2 (plasmid)
Size of vector backbone 5.0 kb
Selectable markers Amp^.
Growth conditions LB+Amp, 37oC
Gene/insert name human GGA3 cDNA
Depositor|Developer Nakayama, Kazuhisa |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Takatsu, H. et al. Biochem. J., 365 (Pt 2): 369-378, 2002). RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Takatsu, H. et al. Biochem. J., 365 (Pt 2): 369-378, 2002)。本件リソースは非営利機関にのみ提供し、利用範囲は学術研究に制限する。

Catalog # Resource name Shipping form Fee
RDB15367 pGEX-4T-2/hGGA3(GAT) DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

How to cite this biological resource

Materials & Methods section:

The pGEX-4T-2/hGGA3(GAT) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15367).

Reference section:

Takatsu, H., Yoshino, K., Toda, K., Nakayama, K., GGA proteins associate with Golgi membranes through interaction between their GGAH domains and ADP-ribosylation factors. Biochem. J. 365 (Pt 2): 369-378 (2002). PMID 11950392. [PubMed] [Article] [RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Takatsu, H., GGA proteins associate with Golgi membranes through interaction between their GGAH domains and ADP-ribosylation factors. Biochem. J. 365 (Pt 2): 369-378 (2002). PMID 11950392. [PubMed] [Article] [RRC of NBRP]