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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pEF-HA-mouse MICAL-L1 (#RDB15385)

Expression vector of mouse (MICAL-L1, N-terminal HA-tag.

Clone info. Expression vector of mouse microtubule associated monooxygenase, calponin and LIM domain containing -like 1 (MICAL-L1), tagged with N-terminal HA, EF1 promoter.
Comment Expression was confirmed by the depositor with immunoprecipitation.
Vector backbone pEF-HA (plasmid)
Size of vector backbone 5.7 kb
Selectable markers Amp^r
Gene/insert name mouse Micall1 cDNA
Depositor|Developer Fukuda, Mitsunori |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kobayashi, H. and Fukuda, M., J. Cell Sci. 126 (11): 2424-2435, 2013)
Additional terms and conditions:
Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by Dr. Shigekazu Nagata (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Kobayashi, H. and Fukuda, M., J. Cell Sci. 126 (11): 2424-2435, 2013)。
MTAに書く付加的使用条件：
pEF-BOS and pEF-BOS-EXベクターについて: 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。利用者が、pEF-BOSおよびpEF-BOS-EXベクターを商業的に利用する場合、利用者は長田重一教授に連絡するものとする。利用者は、研究成果の公表にあたって長田重一教授の指定する文献 (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990.)を引用し、長田重一教授への謝辞の表明を必要とする。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15385 pEF-HA-mouse MICAL-L1 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pEF-HA-mouse MICAL-L1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15385).

Reference section:

Kobayashi, H., Fukuda, M., Rab35 establishes the EHD1-association site by coordinating two distinct effectors during neurite outgrowth. J Cell Sci. 2013 Jun 1;126(Pt 11):2424-2435 (2013). PMID 23572513. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15385_A7Ibp1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F (Pr0168)
Region: EF-1 alpha pro,HA,insert 5'(MICAL-L1)
Sequence file: RDB15385_A7Iba.seq check
>D04916B3_A7Ib_2_EF1a-C_F_G05_20_ABI24.ab1
    1 TAAGCATGAT TTTTGAGTTT GGATCTTGGT TCATTCTCAA GCCTCAGACA GTGGTTCAAA
   61 GTTTTTTTCT TCCATTTCAG GTGTCGTGAG GAATTCTCTA GAGCGGCCGC GGGAATTCGA
  121 TTCCACCATG GGTTACCCAT ACGACGTCCC AGACTACGCT GGATCTATGG CGGGGCCGCG
  181 GGGCGCTCTC CTGGCCTGGT GTCGCCGCCA GTGCGAGGGC TACCGCGGCG TGGACATCCG
  241 CGACCTGAGC AGCTCCTTCC GGGACGGCCT GGCTTTCTGC GCCATCCTGC ACCGGCACCG
  301 GCCCGACCTG CTCGACTTCC AATCTCTCTC CAAGGAAAAT GTCTTCGAGA ATAATCGATT
  361 GGCCTTTGAA GTGGCTGAAA AGGAACTGGG CATCCCTGCT CTCCTGGACC CCAATGACAT
  421 GGTCTCCATG AGTGTCCCTG ACTGTCTCAG CATCATGACC TATGTGTCCC AGTACTACAA
  481 CCACTTCACC AGTTCTGGCC AAGCTGCTGC CTCACCACCC AAACCAGGAA AGGACCCAGC
  541 ACCCCCCTCC CCCACATCCA CATCACCCGC TGTGCAGCCA GGAGAGGAGG CTCAGGGTGA
  601 CGACCTCTCC CCAGACAGCC TGTCCGAGCA GGGCAAACAG CAGCCCCCGA GCAGCGCATG
  661 CGCAGCCTGT GGGCAGCGGG TGCACCTGGT GCAGCGGTAC TTGGCGGAGG GCAGACTCTA
  721 CCACCGGCAC TGCTTCCGAT GTCGGCAGTG TTCCAGCACG CTGGTCCCAG GCTCTTACAG
  781 TAGTGGGCCC GAAGAAGGCA CCTTTGTGTG TGCAGAACGC TGCACCAGGC TGGGTCCGGG
  841 AAGTCGGTCA GGAACTAGGC TCCTTTCACA GCAAAGGCAG CAGCCAGCGG CGGCAGAAGC
  901 TAAAGATGCA GAGGATAATG ACCCAAGCCC TGAGTGTGGC TGCAGTGGCT GAGGCAGACA
  961 GGCTCCAGGG CCAGCCTCGA GTACAGTTTC CACACCCCCA ATGAGCACTC TTCCCAGCCA
1021 GCCCAGCACT GACCAGTCCA CTTGTGACTG ACCAACCTGG CCCCCAGGGA CGGCATC
//
Primer: hGCSF_R (Pr0031)
Region: insert 3'
Sequence file: RDB15385_A7Ibb.seq check
>D04916B3_A7Ib_2_hGCSF_R_C01_03_ABI08.ab1
    1 TTTGGAAACG TTAAAACTCC TCAGGGTCCC AGGGTGGGGA CCCTCACTCT AGAGGCGGCC
   61 GCTATGGTCG ACTCAGCTCT TGTCCCCTGG GGCCTTGCTC TTGGCTTCTC GTTTATTCCC
  121 CAGAAACTTC AGCACCTTTA TGGTCTTGAA CTTGCCTTTC TTCTTACTGT CAGACTCGGC
  181 CTCTCTTTGG AAATCTTTCT TCTTGATCAT AGTTTCCAAC ATCTTGTCTT CCTCCTCTTC
  241 CCTCTGCCGG TCCTCGTCCA GGCAGTTCAC GATGGCATCT CGCTGCTCGA TGAGGGTCAT
  301 GAGTTCCTGC ATCAGCACCT TCTCTCTGGC CCGATCCTCA TCTGTCCAGT CCTTTTCTGG
  361 CTTGTTGAGA AGGCACCGGA GCTCGAACTC CACATCAGCC TGGCGCTGCT CCAGGTTCTG
  421 CTGCTTGAAG ACATAGATGA GCTCCGACTC TCGCCGCACT AGTAGGTGCT TCTCATGGAT
  481 GAGTTTGAAC CAGTCCACCA GCATGTCGTC CTCGCTGCCC TCATTTGCTC CGCCACGCAG
  541 CTTCTCCTCC AACAGAACCC CACTGTGTTC CAGGGCATCC AGCTGGCGCT CAATATTGTC
  601 CATCTCACCG TAGATGTCCT CCTCGGGAAT GTACTGGTCA GCCTGGACCT TGCGTTTGAT
  661 GAGTGGAAAG CCGTGTCCGG GGGCAGGTGG CCTCACAGGC TTGGCTCCTT TTGGTGGCCT
  721 TCCTTACAGT TGGAGACGCG GAGGGAGATG GTTTCCGGTT GAAAGGATTC TCCTTGCAGG
  781 AAGACTTGAT TTGCAGCTGT GGGGATGCGC TCCCCCACCG GGAGGGGCAG GGTTCCGGTC
  841 TCCAGCCCAA CAAAGAAAGG GGTGGGGGGT GGCACCCACC TGAAAGGGCC TTGGTAGAAA
  901 TGGGGTAACC CCCGCGCTGC CCCCTTCGTC CTGGACCCTG GCGGGCATTT TGTTCAACCA
  961 CCTAAGGCTT GCCCCCTAGC CCTCCCCCAA GAAAGACGAC CAGGGTAAAG GAGTTGGGCT
 1021 TGAAATGGGG GGTGAACAGC AGGTTATCCC AAAAGGTGGC CCAAGGGGGT AACCCAGGGC
 1081 CCACCGCTGC CACCAGACCA AGGAATCCCG AAGGGAAAGC TTCCCTTGGG GCTTACCGGC
1141 CACGGCCGGG CCCTCCCAAA AAGAGGCCTT CTTGTCCAAC TTGGTAGGGC CG
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Kobayashi, H., Rab35 establishes the EHD1-association site by coordinating two distinct effectors during neurite outgrowth. J Cell Sci. 2013 Jun 1;126(Pt 11):2424-2435 (2013). PMID 23572513. [link to RRC of NBRP]