Resource data sheet
DNA Bank Top
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pEF-Myc-mouse EHD1 (#RDB15386)

Expression vector of mouse EHD1, N-terminal Myc-tag.

Clone info. Expression vector of mouse EH-domain containing 1 (EHD1), tagged with N-terminal Myc, EF1 promoter.
Comment Expression was confirmed by the depositor with immunoprecipitation and microscopy.
Vector backbone pEF-Myc (plasmid)
Size of vector backbone 5.7 kb
Selectable markers Amp^r
Gene/insert name mouse Ehd1 cDNA
Depositor|Developer Fukuda, Mitsunori |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kobayashi, H. and Fukuda, M., J. Cell Sci. 126 (11): 2424-2435, 2013)
Additional terms and conditions:
Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by Dr. Shigekazu Nagata (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Kobayashi, H. and Fukuda, M., J. Cell Sci. 126 (11): 2424-2435, 2013)。
MTAに書く付加的使用条件：
pEF-BOS and pEF-BOS-EXベクターについて: 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。利用者が、pEF-BOSおよびpEF-BOS-EXベクターを商業的に利用する場合、利用者は長田重一教授に連絡するものとする。利用者は、研究成果の公表にあたって長田重一教授の指定する文献 (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990.)を引用し、長田重一教授への謝辞の表明を必要とする。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15386 pEF-Myc-mouse EHD1 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pEF-Myc-mouse EHD1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15386).

Reference section:

Kobayashi, H., Fukuda, M., Rab35 establishes the EHD1-association site by coordinating two distinct effectors during neurite outgrowth. J Cell Sci. 2013 Jun 1;126(Pt 11):2424-2435 (2013). PMID 23572513. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15386_A7Ibp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F
Sequence file: RDB15386_A7Iba.seq check
>D04916B4_A7Ib_1_EF1a-C_F_F07_16_ABI24.ab1
    1 TCGATCATTG CCTTTTTGAG TTTGGATCTT GGTTCATTCT CAAGCCTCAG ACAGTGGTTC
   61 AAAGTTTTTT TCTTCCATTT CAGGTGTCGT GAGGAATTCT CTAGAGCGGC CGCTCTAGAA
  121 CTAGTGGATC TGCCACCATG GAGCAAAAGC TCATTTCTGA AGAGGACTTG AATGAAGGAT
  181 CCATGTTCAG CTGGGTGAGC AAGGATGCCC GCCGCAAGAA GGAGCCGGAG CTCTTCCAGA
  241 CGGTGGCCGA GGGGCTGCGG CAGCTGTACG CGCAGAAGCT GCTGCCGCTG GAGGAGCACT
  301 ATCGCTTCCA CGAGTTCCAC TCGCCCGCGC TGGAGGACGC TGACTTCGAC AACAAGCCGA
  361 TGGTGCTCCT GGTCGGCCAG TACAGCACCG GCAAGACCAC CTTCATCCGC CACCTGATCG
  421 AGCAGGACTT CCCGGGGATG CGCATCGGGC CGGAGCCCAC CACCGACTCC TTCATCGCGG
  481 TCATGCACGG CCCCACCGAG GGCGTGGTGC CCGGCAACGC GCTCGTCGTG GACCCGCGGC
  541 GCCCCTTCCG CAAGCTCAAC GCCTTCGGCA ACGCCTTCCT CAACAGGTTC ATGTGTGCAC
  601 AGCTGCCCAA CCCAGTACTG GACAGCATCA GCATCATTGA CACTCCTGGG ATCCTGTCTG
  661 GGGAGAAGCA GCGCATCAGC CGAGGTTATG ACTTTGCGGC TGTCCTTGAG TGGTTCGCAG
  721 AGCGTGTGGA CCGCATCATC TTGTTGTTCG ACGCCCACAA GCTGGACATC TCAGACGAGT
  781 TCTCAGAAGT CATCAAGGCC CTCAAAAATC ACGAGGACAA GATCCGTGTG GTGCTGAACA
  841 AGGCTGATCA GATCGAGACG CAGCAGCTGA TGCGAGTATA CGGGGCCCTC ATGTGGTCCC
  901 TGGGGAAGAT CATCAACACC CCCGAGGTGG TCAGAGTCTA CATCGGCTCC TTCTGGGTCA
  961 CACCCACTGC TCATCCCTGA CACCCGGAAA GCTCTTTCGA GGCAGAGGAG CAGGACCTCT
 1021 TCAAAGACAT CCAGTCTCTG CCGAGAAACG TCGCCCTCAG AGCTCATGAC CTCATCCAGC
 1081 GGTCAGCTGG CCAAGGTCAT GCCTACATCA TCAGCTTCCC TCAGAGGAGA ATGCCCAAAT
1141 GATTTTCC
//
Primer: hGCSF_R
Sequence file: RDB15386_A7Ibb.seq check
>D04916B4_A7Ib_1_hGCSF_R_G07_19_ABI24.ab1
    1 GGGGGACTGA TCTCTCAGGG TCCCAGGTGG GGACCCTCAC TCTAGAGCGG CCGCGGGAAT
   61 TCGATTTAGT CGACTCACTC GTGCCTCCGT TTGGAGGGTG GAATGAGATG TGGAGGAAGG
  121 TCAGCGGGCA GCTCGTGGCC CTCTAGCTTC ACCTTGATAA GGTGGTTGGC CAGGGCAAAC
  181 TCCTCGTCAT CCAGCAGGCC ATCCTTGTCC ACATCTGCCA ACTTCCAGAT CTTCCCCAGC
  241 ACTGTGTTGG GCAGCTTGGA CTTCACCATC TCCTTCTTGG CATTAGCACC TGTGATCTTG
  301 CCGTTGACAG GAGACAGTGT GTAGAAGATC TCATCATAGG TGGGCTTGTC CTTGCCAACT
  361 ACCCACTCAA CATCATCAAT GCCCTCGCCA GCCCCCTCGC CGTAGCCATG CCCAAAGGGC
  421 CCATTCATGG TGCCATCAAA AGCACCACCC TTCACAGCCT GTGAGGGCAT CAGGGACTCC
  481 TCCTGGCGCA CCATCACCAT CAGCCGAGCT ATATCGTTGG CCAGCATATC ATCCACTGTA
  541 TCCAGCAGCT TGGGCTTCAA GGCCTGGAAC TTGCTGAAGT CCTGGGTCTG CAGGAGTTCC
  601 TGCATCTTAC GCAGGCTTGG GAAGTCGCCG GAGGAGATCT GGTGCTCCCG CTCGATCTTC
  661 TGGTAGATCT CTCCCAGGTT GTTCACCAGC TCTTTCTTCT TGCTCTCTTT CCCGAAAACA
  721 TTGGGCATCT CCTTCTTGAG GGAGCTGATG ATGTAGGCAT GGACCTTGGC CAGCCTGGCC
  781 CGCTTGATGA GGTCATTGAG CTTCCTGAGG GCGGCGTTTC TCGGCAGAGA CTGGATGTCT
  841 TTGAAGAGGT CCTGCTCCTC TGCCTCGAAG AGCTTCCGGT TGTCAGGGAT GAGCAGTGGG
  901 TGTGACCAGA AGGAGCCGAT GTAGACTCTG ACCACCTCGG GGGTGTTGAT GATCTTCCCC
  961 AGGGACCACA TGAGGGCCCC GTATACTCGC ATCAGCTGCT GCGTCTCGAT CTGATCAGCC
 1021 CTTGTTCAGC ACCACACGGA TCTTGTCCTC GTGATTTTTG AGGGCCTTGA TGACTTTCTG
 1081 AGAACTCGTC TGAGATGTCC AGCTTGTTGG GGGCGTTCGA ACCACCAGGA TGGATGCCGG
1141 TCCACACACG CCTCTTGGCG AAACCAACCT TCCAAGGGAA CAAGAGTCGG GCA
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Kobayashi, H., Rab35 establishes the EHD1-association site by coordinating two distinct effectors during neurite outgrowth. J Cell Sci. 2013 Jun 1;126(Pt 11):2424-2435 (2013). PMID 23572513. [link to RRC of NBRP]