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pMSCV-3xFLAG-hCep63

Retroviral expression vector of human Cep63 tagged with 3xFLAG at N-terminal.

Catalog number RDB15404
Resource name pMSCV-3xFLAG-hCep63
Clone info. Retroviral expression vector of human Cep63 tagged with 3xFLAG at N-terminal.
Comment Expression was confirmed by the depositor with immunoprecipitation.
Vector backbone pMSCV–Flag(3x) (plasmid)
Selectable markers Amp^r (E. coli), Hygro^r (mammalian cells).
Gene/insert name human CEP63 cDNA
Depositor|Developer Shimizu, Shigeomi |
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External Database
human CEP63

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Watanabe, Y. et al. Nat. Commun. 7: 13508, 2016)
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Watanabe, Y. et al. Nat. Commun. 7: 13508, 2016)。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
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          RDB15404 pMSCV-3xFLAG-hCep63 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Watanabe, Y., Autophagy controls centrosome number by degrading Cep63. Nat. Commun. 7: 13508 (2016). PMID 27869116.

          Further references such as user reports and related articles (go to bottom)

          Featured content


          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB15404_A7Ikp1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: gag-M_F
          Region: gag3', 3xFLAG, insert5'
          Sequence file: RDB15404_A7Ika.seq check
          >D04516A4_A7Ik_1_gag-M_F_A06_03_ABI24.ab1
              1 ATTTGGCAGC CTTTGTACAC CCTAAGCCTC CGCCTCCTCT TCCTCCATCC GCCCCGTCTC
             61 TCCCCCTTGA ACCTCCTCGT TCGACCCCGC CTCGATCCTC CCTTTATCCA GCCCTCACTC
            121 CTTCTCTAGG CGCCGGAATT AGATCCATGG ATTACAAGGA TGACGACGAT AAGAGATCCG
            181 ATTACAAGGA TGACGACGAT AAGAGATCCG ATTACAAGGA TGACGACGAT AAGAGATCCA
            241 TGGAGGCTTT GTTAGAAGGA ATACAAAATC GAGGGCATGG TGGGGGATTT TTGACATCTT
            301 GTGAAGCAGA ACTACAGGAG CTCATGAAAC AGATTGACAT AATGGTGGCT CATAAAAAAT
            361 CTGAATGGGA AGGACGTACA CATGCTCTAG AAACTTGCTT GAAAATCCGT GAACAGGAAC
            421 TTAAGAGTCT TAGGAGTCAG TTGGATGTGA CACATAAGGA GGTTGGAATG TTGCATCAGC
            481 AGGTAGAAGA ACATGAAAAA ATCAAGCAAG AGATGACCAT GGAATATAAG CAGGAGTTGA
            541 AGAAACTACA TGAAGAATTA TGCATACTGA AGAGAAGCTA TGAAAAGCTT CAGAAAAAGC
            601 AAATGAGGGA ATTCAGAGGA AATACCAAAA ATCACAGGGA AGATCGGTCT GAAATTGAGA
            661 GGTTAACTGC AAAAATAGAG GAATTCCGTC AGAAATCGCT GGACTGGGAG AAGCAACGCT
            721 TGATTTATCA GCAACAGGTA TCTTCACTGG AGGCACAAAG GAAGGCTCTG GCTGAACAAT
            781 CAGAGATAAT TCAGGCTCAG CTTGTCAATC GGAAACAGAA ATTAGAGTCT GTGGAACTTT
            841 CTAGCCAATC AGAAATTCAA CACTTAAGCA GTAAACTGGA GCGGGCTAAT GACACTATCT
            901 GTGCCAATGA GTTGGAAATA GAGCGCCTCA CCATGAGGGT CAATGACTTG GTTTGGAACC
            961 AGTATGACTG TCCTACAGGG AGCAGCAGCA AAAAGAAGAA AAATTTGAGG ATCTGAAAAA
           1021 CTATTAGAGG GCTCTGCAGA GAAAAGAGAG AATTTGAAGC CAGCTCCTTC CAGTCTCAGA
           1081 TATCTCCATA CATGGAGTCA GGATTACAAA AGAGAGGTAC CAGGAAGTAA GCCTACTTAC
          1141 ATCTTCAGA
          //
          Primer: PGK_N_rev
          Region: insert3'
          Sequence file: RDB15404_A7Ikb.seq check
          >D04516A4_A7Ik_1_PGK_N_rev_B06_06_ABI24.ab1
              1 CCTGGTGCGG CCTCCCTACC CGGTAGATTC GTTAACCTCG AGCTACTTTA AGGCTGTGAA
             61 TTGTCTCACT GTCTTTTCAC TCTCTCTTTT TAGTTCTTCA ATATGGGCAT CCAAGCGCTC
            121 TAGAATGTGA TGAGACCTCA GTTCCTCCTC TTCCAAAAAT CTGGTAGCTA TTGAACCAAG
            181 GGGAGATACA GGCAAGGAAC ACGAAGATAT AAACTCTTCT TGGTCATTCA TACTCTCAGA
            241 CATAGTGTCA GAAAAATTGG CTTCATTTGT ATCTAGCGCT GAAGGCAAAG AATGAGTTTT
            301 ACATAGAGAG TAGGAAGTCA AAGACCTGGT GGAGCTGCAA GGGCTGATTT GAGGACTCAG
            361 GGGGCTTAGC ACCCTAGACA TGGGGTTTAT ACTATCCGAC GCTTGTCCTC TTGGAGAATG
            421 AAGATCTGTT TTGTAGTGCT CAGTCTTTAT TCCATCATGT CTGTGCTGGC CATGGGTTGG
            481 CTTGAACTCT GTATTCTTGA ACTCAGTTGT TCTGCTGTGT GTTGGTTTAA AGATCCTGTC
            541 ATTTTGTTTT TTGCACATCT GAGTAGAAAT CTCCAGCTGA GATTTGGTAT TCATCAAATC
            601 TTTCTGTAGT TGTTGATTTT CAGACACTAA TTTATTTAAT GCCTCAATAT AATTCTCCTG
            661 GAGGTCTGCT AGTGAAATCT CTTTTGCCTC ATGTAAACCC AATTCCAATT TCATCACCAT
            721 TTCAGAAAGA TGACGATTTT CTAATTTGAG TGACTCCAGC TGATCCAAAA TCTCCTTATG
            781 CTCTACTGCT TTGTCTTCTG CCTTTTGCAT CTCTGCTCTG AGTCGCTTTT CCATGTCTGA
            841 GGAAGAACCT TTGGTGGAAG CAATAGTGAT ATCTCGCTGA TGTAACTCCT GAGTTAGATG
            901 GGAGATTTCC ATCTTCATTC CTTCTAGTGC AGAACTGTAA CTTTGTTCAC CCTGTAAAAT
            961 CTGTTCTTTC AACTTCTTAA TCTCTGCTTT GTATTCATTG TTATGTGCTT CCATCCCTCT
           1021 TTCAGTTTCT TTCATATTTT TCCATTTAGT TCTTGGCTCA GCTGTTTACA CGTTGCACTC
           1081 ACAGATTCCC AAACTGCCTC CAGACAGGTT CAACGTCTGC CCTGCAGAAG GTCCCTCAAC
          1141 TTAGTAATTG
          //
          Primer: pBR322_BamHI_R
          Region: 5'LTR, MESV psi
          Sequence file: RDB15404_A7Ikc.seq check
          >D04516A4_A7Ik_1_pBR322_BamHI_R_C06_09_ABI24.ab1
              1 TGTGGATAGG AAGGGATATC AGTGGTCCAG GCTCTAGTTT TGACTCAACA ATATCACCAG
             61 CTGAAGCCTA TAGAGTACGA GCCATAGATA AAATAAAAGA TTTTATTTAG TCTCCAGAAA
            121 AAGGGGGGAA TGAAAGACCC CACCTGTAGG TTTGGCAAGC TAGCTTAAGT AACGCCATTT
            181 TGCAAGGCAT GGAAAATACA TAACTGAGAA TAGAGAAGTT CAGATCAAGG TTAGGAACAG
            241 AGAGACAGCA GAATATGGGC CAAACAGGAT ATCTGTGGTA AGCAGTTCCT GCCCCGGCTC
            301 AGGGCCAAGA ACAGATGGTC CCCAGATGCG GTCCCGCCCT CAGCAGTTTC TAGAGAACCA
            361 TCAGATGTTT CCAGGGTGCC CCAAGGACCT GAAATGACCC TGTGCCTTAT TTGAACTAAC
            421 CAATCAGTTC GCTTCTCGCT TCTGTTCGCG CGCTTCTGCT CCCCGAGCTC AATAAAAGAG
            481 CCCACAACCC CTCACTCGGC GCGCCAGTCC TCCGATAGAC TGCGTCGCCC GGGTACCCGT
            541 ATTCCCAATA AAGCCTCTTG CTGTTTGCAT CCGAATCGTG GACTCGCTGA TCCTTGGGAG
            601 GGTCTCCTCA GATTGATTGA CTGCCCACCT CGGGGGTCTT TCATTTGGAG GTTCCACCGA
            661 GATTTGGAGA CCCCTGCCCA GGGACCACCG ACCCCCCCGC CGGGAGGTAA GCTGGCCAGC
            721 GGTCGTTTCG TGTCTGTCTC TGTCTTTGTG CGTGTTTGTG CCGGCATCTA ATGTTTGCGC
            781 CTGCGTCTGT ACTAGTTAGC TAACTAGCTC TGTATCTGGC GGACCCGTGG TGGAACTGAC
            841 GAGTTCTGAA CACCCGGCCG CAACCCTGGG AGACGTCCCA GGGACTTTGG GGGGCCGTTT
            901 TTGTGGCCCG ACCTGAGGAA GGGAGTCGAT GTGGAAATCC GACCCCGTCA GGATATGTGG
            961 TTCTGGTAGG AGACGAGAAC CTAAACAGTT TCCCGCCTCC GTCTGAATTT TTGCTTTTCG
           1021 CTTGGAACCC GAAGCCGCGC GTCTGTCTGC CTGCAGCCGC TGCAGCATCG TTCCTGTGGT
           1081 GGTCTCTTGT CTGACTGTGT TTCTGTATTG TCTGGAAATT AGGGCCCAGA CTGGTTACCC
          1141 ACTTCCTCTT ATAAGTTGA
          //
          Primer: PGKpro_F
          Region: PGK pro, HygR 5'
          Sequence file: RDB15404_A7Ikd.seq check
          >D04516A4_A7Ik_1_PGKpro_F_B07_04_ABI24.ab1
              1 ATTGGCGTAT CTCTCTTCTC ATCTCCGGGC CTTTCGACCT GCAGCCCAAG CTTATGAAAA
             61 AGCCTGAACT CACCGCGACG TCTGTCGAGA AGTTTCTGAT CGAAAAGTTC GACAGCGTCT
            121 CCGACCTGAT GCAGCTCTCG GAGGGCGAAG AATCTCGTGC TTTCAGCTTC GATGTAGGAG
            181 GGCGTGGATA TGTCCTGCGG GTAAATAGCT GCGCCGATGG TTTCTACAAA GATCGTTATG
            241 TTTATCGGCA CTTTGCATCG GCCGCGCTCC CGATTCCGGA AGTGCTTGAC ATTGGGGAGT
            301 TCAGCGAGAG CCTGACCTAT TGCATCTCCC GCCGTGCACA GGGTGTCACG TTGCAAGACC
            361 TGCCTGAAAC CGAACTGCCC GCTGTTCTGC AGCCGGTCGC GGAGGCCATG GATGCGATCG
            421 CTGCGGCCGA TCTTAGCCAG ACGAGCGGGT TCGGCCCATT CGGACCGCAA GGAATCGGTC
            481 AATACACTAC ATGGCGTGAT TTCATATGCG CGATTGCTGA TCCCCATGTG TATCACTGGC
            541 AAACTGTGAT GGACGACACC GTCAGTGCGT CCGTCGCGCA GGCTCTCGAT GAGCTGATGC
            601 TTTGGGCCGA GGACTGCCCC GAAGTCCGGC ACCTCGTGCA CGCGGATTTC GGCTCCAACA
            661 ATGTCCTGAC GGACAATGGC CGCATAACAG CGGTCATTGA CTGGAGCGAG GCGATGTTCG
            721 GGGATTCCCA ATACGAGGTC GCCAACATCT TCTTCTGGAG GCCGTGGTTG GCTTGTATGG
            781 AGCAGCAGAC GCGCTACTTC GAGCGGAGGC ATCCGGAGCT TGCAGGATCG CCGCGGCTCC
            841 GGGCGTATAT GCTCCGCATT GGTCTTGACC AACTCTATCA GAGCTTGGTT GACGGCAATT
            901 TCGATGATGC AGCTTGGGCG CAGGGTCGAT GCGACGCAAT CGTCCGATCC GGAGCCGGGA
            961 CTGTCGGGCG TACACAAATC GCCCGCAGAA GCGCGGGCCG TCTGGACCCG ATGGCTGTGT
           1021 AGAAAGTACT CGCCGATAGT GGAAACCCGA CGCCCCAGCA CTCGTCCGAG GGCAAAGGTA
          1081 TAGATTCGGA TAAATTAAAA GAATTTTTAA TTTTAAGTTC TTTCCAAGAA AAAAAA
          //
          Primer: HygR-C_F
          Region: HygR 3', 3'LTR
          Sequence file: RDB15404_A7Ike.seq check
          >D04516A4_A7Ik_1_HygR-C_F_E02_05_ABI08.ab1
              1 CGAATCGGGG GGATCCAATA CGAGGTCGCC AACATCTTCT TCTGGAGGCC GTGGTTGGCT
             61 TGTATGGAGC AGCAGACGCG CTACTTCGAG CGGAGGCATC CGGAGCTTGC AGGATCGCCG
            121 CGGCTCCGGG CGTATATGCT CCGCATTGGT CTTGACCAAC TCTATCAGAG CTTGGTTGAC
            181 GGCAATTTCG ATGATGCAGC TTGGGCGCAG GGTCGATGCG ACGCAATCGT CCGATCCGGA
            241 GCCGGGACTG TCGGGCGTAC ACAAATCGCC CGCAGAAGCG CGGCCGTCTG GACCGATGGC
            301 TGTGTAGAAG TACTCGCCGA TAGTGGAAAC CGACGCCCCA GCACTCGTCC GAGGGCAAAG
            361 GAATAGATCG ATAAAATAAA AGATTTTATT TAGTCTCCAG AAAAAGGGGG GAATGAAAGA
            421 CCCCACCTGT AGGTTTGGCA AGCTAGCTTA AGTAACGCCA TTTTGCAAGG CATGGAAAAT
            481 ACATAACTGA GAATAGAGAA GTTCAGATCA AGGTTAGGAA CAGAGAGACA GCAGAATATG
            541 GGCCAAACAG GATATCTGTG GTAAGCAGTT CCTGCCCCGG CTCAGGGCCA AGAACAGATG
            601 GTCCCCAGAT GCGGTCCCGC CCTCAGCAGT TTCTAGAGAA CCATCAGATG TTTCCAGGGT
            661 GCCCCAAGGA CCTGAAATGA CCCTGTGCCT TATTTGAACT AACCAATCAG TTCGCTTCTC
            721 GCTTCTGTTC GCGCGCTTCT GCTCCCCGAG CTCAATAAAA GAGCCCACAA CCCCTCACTC
            781 GGCGCGCCAG TCCTCCGATA GACTGCGTCG CCCGGGTACC CGTGTATCCA ATAAACCCTC
            841 TTGCAGTTGC ATCCGACTTG TGGTCTCGCT GTTCCTTGGG AGGGTCTCCT CTGAGTGATT
            901 GACTACCCGT CAGCGGGGGG TCTTTCATGG GTAACAGTTT CTTGAAGTTG GGAGAACAAC
            961 ATTCTGAGGG TAGGAGTCGG AATATTAAAG TAATCCTGAC TCATTTAGCC ACTGGTTTTG
           1021 AATCCACATA CTCCCAATAC TTCCTTGAAT TAGGTTCCAT TATGGACAGC GCAGAGAAGC
          1081 CTGGGGGGAG GAATTGCTGC AAAACTTG
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Watanabe, Y., Autophagy controls centrosome number by degrading Cep63. Nat. Commun. 7: 13508 (2016). PMID 27869116.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl