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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pCAGIP-Gpnmb-FLAG/HA

Expression vector of mouse glycoprotein nmb (GPNMB).

Catalog number RDB15413
Resource name pCAGIP-Gpnmb-FLAG/HA
Clone info. Expression vector of mouse glycoprotein nmb (GPNMB), tagged with FLAG and HA at C-terminal, SV40 ori, IRES-puromycin resistance, CAG promoter.
Comment Expression was confirmed by the depositor with immunoblotting and immunoprecipitation. Please refer Niwa, H. et al., Nat. Genet. 24 (4): 372-376, 2000 for pCAGIP vector.
Vector backbone pCAGIP (Plasmid)
Selectable markers Amp^r (E. coli), Puro^r (mammalian cells).
Gene/insert name Mouse Gpnmb cDNA
Depositor|Developer Kato, Mitsuyasu |
Other clones in our bank

External Database
Mouse Gpnmb

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Okita, Y et al. Sci. Signal. 10(474) pii: eaak9397, 2017).
          Additional terms and conditions for distribution Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
          Ordering Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Okita, Y et al. Sci. Signal. 10(474) pii: eaak9397, 2017)。
          付加的提供条件 CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

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          RDB15413 pCAGIP-Gpnmb-FLAG/HA DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Okita, Y., The transcription factor MAFK induces EMT and malignant progression of triple-negative breast cancer cells through its target GPNMB. Sci. Signal 10 (474). pii: eaak9397 (2017). PMID 28400538.

          Further references such as user reports and related articles (go to bottom)

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          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB15413_A7J2p1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pCAG-F_V2
          Sequence file: RDB15413_A7J2a.seq check
          >D04907A2_A7J2_1_pCAG-F_V2_B04_02_ABI08.ab1
              1 GAATACTTTG GTTCAAATCT GTGCGGAGCC GAATCTGGGA GGCGCCGCCG CACCCCCTCT
             61 AGCGGGCGCG GGGCGAAGCG GTGCGGCGCC GGCAGGAAGG AAATGGGCGG GGAGGGCCTT
            121 CGTGCGTCGC CGCGCCGCCG TCCCCTTCTC CCTCTCCAGC CTCGGGGCTG TCCGCGGGGG
            181 GACGGCTGCC TTCGGGGGGG ACGGGGCAGG GCGGGGTTCG GCTTCTGGCG TGTGACCGGC
            241 GGCTCTAGAG CCTCTGCTAA CCATGTTCAT GCCTTCTTCT TTTTCCTACA GCTCCTGGGC
            301 AACGTGCTGG TTATTGTGCT GTCTCATCAT TTTGGCAAAG AATTCAGATC TGCCGCCATG
            361 GAAAGTCTCT GCGGGGTCCT GGGATTTCTG CTGCTGGCTG CAGGACTGCC TCTCCAGGCT
            421 GCCAAGCGAT TTCGTGATGT GCTGGGCCAT GAACAGTATC CCGATCACAT GAGAGAGCAC
            481 AACCAATTAC GTGGCTGGTC TTCGGATGAA AATGAATGGG ATGAACACCT GTATCCAGTG
            541 TGGAGGAGGG GAGACGGCAG GTGGAAGGAC TCCTGGGAAG GAGGCCGTGT GCAGGCAGTC
            601 CTGACCAGTG ACTCACCGGC TCTGGTGGGT TCCAATATCA CTTTTGTGGT GAACCTGGTG
            661 TTCCCCAGAT GCCAGAAGGA AGATGCTAAT GGCAATATCG TCTATGAGAA GAACTGCAGG
            721 AATGATTTGG GACTGACATC TGACCTGCAT GTCTACAACT GGACTGCAGG GGCAGATGAT
            781 GGTGACTGGG AAGATGGCAC CAGCCGAAGC CAGCATCTCA GGTTCCCGGA CAGGAGGCCC
            841 TTCCCTCGCC CCCATGGATG GAAGAAAATG GAGCTTTGTC TACGTCTTTC ACACACTTGG
            901 CCAGTATTTC CAAAAACTGG GTCGGTGTTC AGCACGGGGT TTCTATAACA CAGTCAACTT
            961 GACAGCTGGC CCTCAAGGTC ATGGAAAGTG ACTGTCTTTT CGAAGATTAC GGCCCGGGGC
           1021 ATTACATTTC CTCATCTCGG AAGGTGAAAA GATTGTGTAT TGGTGAATAA CAGATCAGGA
           1081 TCCCTTGTAA TTCGGTGACC CATGTCCCAG AGGATTGAAC AGGAACTTGG TCTGAATGGA
           1141 GATCCTTTCC CTTCGAAGAG AACCCTCCCT CATCGCGTCC CTTCGCGAAT TGTAGTGTCC
          1201 TCA
          //
          Primer: IRES-R
          Sequence file: RDB15413_A7J2b.seq check
          >D04907A2_A7J2_1_IRES-R_C04_03_ABI08.ab1
              1 GCCACATACC GGGCCTTTCG GGCCAGTTAA CGTTAGGGGG GGGGGGAGGG AGAGGGGGGG
             61 GGGGCGGAAT TCCTCGAGCT AGAGGCTAGC ATAATCAGGA ACATCATACG GATACTTGTC
            121 ATCGTCGTCC TTGTAGTCGC GGCCGCAGAG TGTCCTTGGC TTGTCCTGGA GCAATGGATC
            181 CTTCTCCTGG TCTCCTCGGA AGAACGGGGC TTTCGCGTGA CTGAGGAGAA CACTCAGGCC
            241 CTTGCCCTTG ACCGTGTTCC TGGGGCAGTT TCCTATTGGC TTGTACGCCT TGTGTTTTTT
            301 GTACAGCAAG ATGGTAACCA TGGTGACAAG CACAGCCAGG CAGCCAATGG AGATCAGGAC
            361 ACCATTCACT GCTCTCAGAG GGGAGTCTGG GTCTTTGCCA GGGATAGAGA TCAGGGTGCT
            421 GGTGAGGGCC AGGCTTGCAT CATCTCCCAG AGTGAAATTC ACACAGTAGG TGCCAGACCC
            481 ATTGAAGGCT CTTCTCACAG ACAGCAGGCA CAGCCCATCC ACAGCCACAG GGCTGCAAAC
            541 CCGGTTCTGG GCGATCTGGC AGGTGGGGTC GGAGATGATC GTACAGGCTT CCATGGGGGT
            601 GGCCCCTTTG CAGGTCACAG TGAAGTCCAT CAGGGAGTTG GCAGGCTGCG GTGTGGGCAT
            661 GGGGACATCT GCTATCTGCA TGATGCTGAC TTCCAGGATC CCCTCTACAA TTGTGATGGT
            721 GGCTCTGAAG TAGCCAATAT CTGTTTATTC GGCAGTTTTC ATTGGAAAAT GTCACTCAGC
            781 TCCATGGATT TGTAACCAGT AGGCATTAAA GAGGGGCTAG GGTGTTGATA ATGTGGGCAG
            841 AGGTTGAGGG CGGAGGTGAG GGGCGGAGTT GAAGGTGGAG GCGGAGTCCG AAAGGCGAAG
            901 GGGGAGGGGC ATGGCCCCGG GCACTGCAGT TTTGCACGGT GAGGTTAAAG GTTTGAAAGG
            961 TTCCCATTTG AGCACCATAA GTTGTGATTT CAAAGTGGTG ATTTGTTTGT AAGACAAACA
           1021 GGCCCAGTTG TTTGTTCCCC CAAAGGTTTC CACCTTTGTC AGGAAAAATG GCCGAAGTTC
           1081 GTTTTGAGGA AAGTTGGCCT TGGGGATTCA TGTGAATATG AAGGGACATC TCGAAAGAAA
           1141 ACGAATTGGG GGGAAGGTTC TTCTGTAAGG GAAAAATTCT TCATTCAGGA CCAAGATTTC
          1201 TCCTCTGTGC TTCCCTATCT CTTCTCTCTG TGGA
          //
          Primer: IRES-F
          Sequence file: RDB15413_A7J2c.seq check
          >D04907A2_A7J2_1_IRES-F_D04_04_ABI08.ab1
              1 TACTAGCCTT TTTATGGTTG TTTTAGGTCG AGGTTAAAAA AACGTCTAGG CCCCCCGAAC
             61 CACGGGGACG TGGTTTTCCT TTGAAAAACA CGATGATAAT ATGGCCACAA CCATGACCGA
            121 GTACAAGCCC ACGGTGCGCC TCGCCACCCG CGACGACGTC CCCAGGGCCG TACGCACCCT
            181 CGCCGCCGCG TTCGCCGACT ACCCCGCCAC GCGCCACACC GTCGATCCGG ACCGCCACAT
            241 CGAGCGGGTC ACCGAGCTGC AAGAACTCTT CCTCACGCGC GTCGGGCTCG ACATCGGCAA
            301 GGTGTGGGTC GCGGACGACG GCGCCGCGGT GGCGGTCTGG ACCACGCCGG AGAGCGTCGA
            361 AGCGGGGGCG GTGTTCGCCG AGATCGGCCC GCGCATGGCC GAGTTGAGCG GTTCCCGGCT
            421 GGCCGCGCAG CAACAGATGG AAGGCCTCCT GGCGCCGCAC CGGCCCAAGG AGCCCGCGTG
            481 GTTCCTGGCC ACCGTCGGCG TCTCGCCCGA CCACCAGGGC AAGGGTCTGG GCAGCGCCGT
            541 CGTGCTCCCC GGAGTGGAGG CGGCCGAGCG CGCCGGGGTG CCCGCCTTCC TGGAGACCTC
            601 CGCGCCCCGC AACCTCCCCT TCTACGAGCG GCTCGGCTTC ACCGTCACCG CCGACGTCGA
            661 GGTGCCCGAA GGACCGCGCA CCTGGTGCAT GACCCGCAAG CCCGGTGCCT GACGCCCGCC
            721 CCACGACCCG CAGCGCCCGA CCGAAAGGAG CGCACGACCC CATGCATCGA TGATCTAGAG
            781 CTCGCTGATC AGCCTCGACT GTGCCTTCTA GTTGCCAGCC ATCTGTTGTT TGCCCCTCCC
            841 CCGTGCCTTC CTTGACCCTG GAAGGTGCCA CTCCCACTGT CCTTTCCTAA TAAATGAGGA
            901 AATTGCATCG CATTGTCTGA GTAGGTGTCA TTCTATTCTG GGGGGTGGGG TGGGCAGGAC
            961 AGCAAGGGGG AGGATTGGGA AGACAATAGC AGGCATGCTG GGGATGCGGT GGGCTCTATG
           1021 GCTTCTGAGG CGGAAAGAAC CTTGCAGCCC AAGCTTGGCG TAAATCATGG GTCAATAAGC
           1081 CTGTTTCCCT GTGTGAAAAT TTGATAATTC CGCCTTCCAC AATTCACACA CATACCGAGT
          1141 CGGGAGGCAA TTAAAGTGTA AGCCCTTGGG GGTGCCCTTA ATG
          //
          Primer: IRES-F
          Sequence file: RDB15413_A7J2c.seq check
          >D04907A2_A7J2_1_IRES-F_D04_04_ABI08.ab1
              1 TACTAGCCTT TTTATGGTTG TTTTAGGTCG AGGTTAAAAA AACGTCTAGG CCCCCCGAAC
             61 CACGGGGACG TGGTTTTCCT TTGAAAAACA CGATGATAAT ATGGCCACAA CCATGACCGA
            121 GTACAAGCCC ACGGTGCGCC TCGCCACCCG CGACGACGTC CCCAGGGCCG TACGCACCCT
            181 CGCCGCCGCG TTCGCCGACT ACCCCGCCAC GCGCCACACC GTCGATCCGG ACCGCCACAT
            241 CGAGCGGGTC ACCGAGCTGC AAGAACTCTT CCTCACGCGC GTCGGGCTCG ACATCGGCAA
            301 GGTGTGGGTC GCGGACGACG GCGCCGCGGT GGCGGTCTGG ACCACGCCGG AGAGCGTCGA
            361 AGCGGGGGCG GTGTTCGCCG AGATCGGCCC GCGCATGGCC GAGTTGAGCG GTTCCCGGCT
            421 GGCCGCGCAG CAACAGATGG AAGGCCTCCT GGCGCCGCAC CGGCCCAAGG AGCCCGCGTG
            481 GTTCCTGGCC ACCGTCGGCG TCTCGCCCGA CCACCAGGGC AAGGGTCTGG GCAGCGCCGT
            541 CGTGCTCCCC GGAGTGGAGG CGGCCGAGCG CGCCGGGGTG CCCGCCTTCC TGGAGACCTC
            601 CGCGCCCCGC AACCTCCCCT TCTACGAGCG GCTCGGCTTC ACCGTCACCG CCGACGTCGA
            661 GGTGCCCGAA GGACCGCGCA CCTGGTGCAT GACCCGCAAG CCCGGTGCCT GACGCCCGCC
            721 CCACGACCCG CAGCGCCCGA CCGAAAGGAG CGCACGACCC CATGCATCGA TGATCTAGAG
            781 CTCGCTGATC AGCCTCGACT GTGCCTTCTA GTTGCCAGCC ATCTGTTGTT TGCCCCTCCC
            841 CCGTGCCTTC CTTGACCCTG GAAGGTGCCA CTCCCACTGT CCTTTCCTAA TAAATGAGGA
            901 AATTGCATCG CATTGTCTGA GTAGGTGTCA TTCTATTCTG GGGGGTGGGG TGGGCAGGAC
            961 AGCAAGGGGG AGGATTGGGA AGACAATAGC AGGCATGCTG GGGATGCGGT GGGCTCTATG
           1021 GCTTCTGAGG CGGAAAGAAC CTTGCAGCCC AAGCTTGGCG TAAATCATGG GTCAATAAGC
           1081 CTGTTTCCCT GTGTGAAAAT TTGATAATTC CGCCTTCCAC AATTCACACA CATACCGAGT
          1141 CGGGAGGCAA TTAAAGTGTA AGCCCTTGGG GGTGCCCTTA ATG
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Okita, Y., The transcription factor MAFK induces EMT and malignant progression of triple-negative breast cancer cells through its target GPNMB. Sci. Signal 10 (474). pii: eaak9397 (2017). PMID 28400538.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl