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pcDNA3.1(+)-c.C225R

Expression vector of mouse transforming growth factor-beta 1 (Tgfb1), C225R missense mutation.

Catalog number RDB15440
Resource name pcDNA3.1(+)-c.C225R
Clone info. Expression vector of mouse transforming growth factor-beta 1 (Tgfb1), C225R (c.637T>A) missense mutation. Total RNA was extracted from mouse embryos at embryonic day 18.5.
Comment Expression was confirmed by the depositor with western blotting.
Vector backbone pcDNA3.1(+) (plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells).
Gene/insert name mouse Tgfb1 cDNA
Depositor|Developer Furuichi, Tatsuya |
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External Database
mouse Tgfb1

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Ichimura, S et al. Exp. Anim. 66 (2) : 137-144, 2017).
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Ichimura, S et al. Exp. Anim. 66 (2) : 137-144, 2017)。
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          RDB15440 pcDNA3.1(+)-c.C225R DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Ichimura, S., An ENU-induced p.C225S missense mutation in the mouse Tgfb1 gene does not cause Camurati-Engelmann disease-like skeletal phenotypes. Exp. Anim. 66 (2): 137-144 (2017). PMID 27928112.

          Further references such as user reports and related articles (go to bottom)

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          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB15440_A7Jgp1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV-Forward
          Sequence file: RDB15440_A7Jga.seq check
          >D04886A3_A7Jg_1_CMV-Forward_G03_21_ABI24.ab1
              1 CTTACTCCGC CCATTGACGC AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC
             61 AGAGCTCTCT GGCTAACTAG AGAACCCACT GCTTACTGGC TTATCGAAAT TAATACGACT
            121 CACTATAGGG AGACCCAAGC TGGCTAGCGT TTAAACTTAA GCTTGGTACC GAGCTCGGAT
            181 CCACTAGTCC AGTGTGGTGG AATTCATCCC ACCTTTGCCG AGGGTTCCCG CTCTCCGAAG
            241 TGCCGTGGGG CGCCGCCTCC CCCATGCCGC CCTCGGGGCT GCGGCTACTG CCGCTTCTGC
            301 TCCCACTCCC GTGGCTTCTA GTGCTGACGC CCGGGAGGCC AGCCGCGGGA CTCTCCACCT
            361 GCAAGACCAT CGACATGGAG CTGGTGAAAC GGAAGCGCAT CGAAGCCATC CGTGGCCAGA
            421 TCCTGTCCAA ACTAAGGCTC GCCAGTCCCC CAAGCCAGGG GGAGGTACCG CCCGGCCCGC
            481 TGCCCGAGGC GGTGCTCGCT TTGTACAACA GCACCCGCGA CCGGGTGGCA GGCGAGAGCG
            541 CCGACCCAGA GCCGGAGCCC GAAGCGGACT ACTATGCTAA AGAGGTCACC CGCGTGCTAA
            601 TGGTGGACCG CAACAACGCC ATCTATGAGA AAACCAAAGA CATCTCACAC AGTATATATA
            661 TGTTCTTCAA TACGTCAGAC ATTCGGGAAG CAGTGCCCGA ACCCCCATTG CTGTCCCGTG
            721 CAGAGCTGCG CTTGCAGAGA TTAAAATCAA GTGTGGAGCA ACATGTGGAA CTCTACCAGA
            781 AATATAGCAA CAATTCCTGG CGTTACCTTG GTAACCGGCT GCTGACCCCC ACTGATACGC
            841 CTGAGTGGCT GTCTTTTGAC GTCACTGGAG TTGTACGGCA GTGGCTGAAC CAAGGAGACG
            901 GAAATACAGG GCTTTCGATT CAGCGCTCAC TGCTCTCGTG ACAGCAAAGA TAACAACTCC
            961 ACGTGGTAAA TCAACGGGGA TCAGCCCCCA AACGTCTGGG CGACCCTGGG GCACCATCCC
           1021 ATGACATGAA CCCGGCCCTT CCTGCCTCAT CATTGACCAC CCCCCCGTGG CAAGGACCAA
          1081 GCAACTTGCA CAAGCTCCAC GGCCAAACCC
          //
          Primer: BGH_rev2
          Sequence file: RDB15440_A7Jgb.seq check
          >D04886A3_A7Jg_1_BGH_rev2_H03_24_ABI24.ab1
              1 CATCGTTAGA GCACGGGGGA GGGGCAACAA CAGATGGCTG GCAACTAGAA GGCACAGTCG
             61 AGGCTGATCA GCGGGTTTAA ACGGGCCCTC TAGACTCGAG CGGCCGCTCA GCTGCACTTG
            121 CAGGAGCGCA CAATCATGTT GGACAACTGC TCCACCTTGG GCTTGCGACC CACGTAGTAG
            181 ACGATGGGCA GTGGCTCCAA AGCCTGCGGC ACGCAGCACG GTGACGCCGA AGCGCCCGGG
            241 TTGTGTTGGT TGTAGAGGGC AAGGACCTTG CTGTACTGTG TGTCCAGGCT CCAAATATAG
            301 GGGCAGGGTC CCAGACAGAA GTTGGCATGG TAGCCCTTGG GCTCGTGGAT CCACTTCCAA
            361 CCCAGGTCCT TCCTAAAGTC AATGTACAGC TGCCGCACAC AGCAGTTCTT CTCTGTGGAG
            421 CTGAAGCAAT AGTTGGTATC CAGGGCTCTC CGGTGCCGTG AGCTGTGCAG GTGCTGGGCC
            481 CTTTCCAGGG GGGTGGCCAT GAGGAGCAGG AAGGGCCGGT TCATGTCATG GATGGTGCCC
            541 AGGTCGCCCC GACGTTTGGG GCTGATCCCG TTGATTTCCA CGTGGAGTTT GTTATCTTTG
            601 CTGTCACGAG AGCAGTGAGC GCTGAATCGA AAGCCCTGTA TTCCGTCTCC TTGGTTCAGC
            661 CACTGCCGTA CAACTCCAGT GACGTCAAAA GACAGCCACT CAGGCGTATC AGTGGGGGTC
            721 AGCAGCCGGT TACCAAGGTA ACGCCAGGAA TTGTTGCTAT ATTTCTGGTA GAGTTCCACA
            781 TGTTGCTCCA CACTTGATTT TAATCTCTGC AAGCGCAGCT CTGCACGGGA CAGCAATGGG
            841 GGTTCGGGCA CTGCTTCCCG AATGTCTGAC GTATTGAAGA ACATATATAT ACTGTGTGAG
            901 ATGTCTTTGG TTTTCTCATA GATGGCGTTG TTGCGGTCCA CCATTAGCAC GCGGGTGACC
            961 CTCTTTAGCA TAGTAGTCCG CTTTCGGGCT CCGGGCTCTG GGTCGGGCGC TCTCGCCTGG
           1021 CCACCCGGTC GCGGTGCTGT TGTACCAAGC CGAGCAACGC TCGGGCAGCG GTCGGTCGTT
          1081 AGTCCCCCTT GACTTGGGGG GAACTGACCT GA
          //
          Primer: SV40pro_F
          Sequence file: RDB15440_A7Jgc.seq check
          >D04886A3_A7Jg_1_SV40pro_F_H02_23_ABI24.ab1
              1 GCTGGTCATT GCATTCAGAG TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA
             61 AGCTCCCGGG AGCTTGTATA TCCATTTTCG GATCTGATCA AGAGACAGGA TGAGGATCGT
            121 TTCGCATGAT TGAACAAGAT GGATTGCACG CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC
            181 TATTCGGCTA TGACTGGGCA CAACAGACAA TCGGCTGCTC TGATGCCGCC GTGTTCCGGC
            241 TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG TCAAGACCGA CCTGTCCGGT GCCCTGAATG
            301 AACTGCAGGA CGAGGCAGCG CGGCTATCGT GGCTGGCCAC GACGGGCGTT CCTTGCGCAG
            361 CTGTGCTCGA CGTTGTCACT GAAGCGGGAA GGGACTGGCT GCTATTGGGC GAAGTGCCGG
            421 GGCAGGATCT CCTGTCATCT CACCTTGCTC CTGCCGAGAA AGTATCCATC ATGGCTGATG
            481 CAATGCGGCG GCTGCATACG CTTGATCCGG CTACCTGCCC ATTCGACCAC CAAGCGAAAC
            541 ATCGCATCGA GCGAGCACGT ACTCGGATGG AAGCCGGTCT TGTCGATCAG GATGATCTGG
            601 ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG AACTGTTCGC CAGGCTCAAG GCGCGCATGC
            661 CCGACGGCGA GGATCTCGTC GTGACCCATG GCGATGCCTG CTTGCCGAAT ATCATGGTGG
            721 AAAATGGCCG CTTTTCTGGA TTCATCGACT GTGGCCGGCT GGGTGTGGCG GACCGCTATC
            781 AGGACATAGC GTTGGCTACC CGTGATATTG CTGAAGAGCT TGGCGGCGAA TGGGCTGACC
            841 GCTTCCTCGT GCTTTACGGT ATCGCCGCTC CCGATTCGCA GCGCATCGCC TTCTATCGCC
            901 TTCTTGACGA GTTCTTCTGA GCGGGACTCT GGGGGTTCGA AATGACCGAC CAAGCGACGC
            961 CCAACCCTGC CCATCACGAG ATTTCGATTC CACCGCCGCC TTTCTATGAA AGGTTGGGCT
           1021 TCGGAATCGG TTTTTCCGGG ACGCCGGCTG GATGAATCTT CAGCGCGGGG ATTCTTCATG
          1081 CTGCAGTCCA TCGCCCAACC CCGACTTGCA TTATTTGCCA G
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Ichimura, S., An ENU-induced p.C225S missense mutation in the mouse Tgfb1 gene does not cause Camurati-Engelmann disease-like skeletal phenotypes. Exp. Anim. 66 (2): 137-144 (2017). PMID 27928112.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl