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mCherry-hCdt1(1/100)/pCSII-EF

Lentivirus vector plasmid of mCherry-hCdt1(1/100). EF-1 alpha promoter.

Catalog number RDB15442
Resource name mCherry-hCdt1(1/100)/pCSII-EF
Clone info. Lentivirus vector plasmid of mCherry-hCdt1(1/100). EF-1 alpha promoter.
Comment Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Vector backbone CSII-EF-MCS (plasmid)
Size of vector backbone 9.1 kb
Selectable markers Please note that Zeo resistance marker is not included in the lentivirus produced from this vector. E. coli: Amp^r.
Gene/insert name Human CDT1 cDNA Discosoma sp. RFP cDNA
Reference of insert sequence LC192885 (DDBJ accession number)
Depositor|Developer Miyawaki, Atsushi |
Other clones in our bank

External Database
Human CDT1 Discosoma sp. RFP

          Reference sequence
            
          Lentiviral plasmid list. [link]

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR 1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
          2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
          Sakaue-Sawano, A., Yo, M., Komatsu, N., Hiratsuka, T., Kogure, T., Hoshida, T., Goshima, N., Matsuda, M., Miyoshi, H., Miyawaki, A. (2017) Genetically-Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5.
          3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
          4. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
          5. The RECIPIENT shall obtain written approval from the UCSD using the MTA provided by the Technology Transfer office of UCSD.
          Additional terms and conditions for distribution The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer of the lentivirus vector.
          Ordering Please visit Ordering instruction.[link] 
          Order form 
          [Credit Card Payment]  [Bank Transfer Payment]
          Material Transfer Agreement (MTA, for use for not-for-profit academic purpose)[Word]
          Remarks Remember that you will be working with samples containing infectious virus.
          This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.
          提供案内 (日本国内) [open/close]

          提供条件 1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
          2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
          Sakaue-Sawano, A., Yo, M., Komatsu, N., Hiratsuka, T., Kogure, T., Hoshida, T., Goshima, N., Matsuda, M., Miyoshi, H., Miyawaki, A. (2017) Genetically-Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5.
          3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
          4. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
          5. The RECIPIENT shall obtain written approval from the UCSD using the MTA provided by the Technology Transfer office of UCSD.
          付加的提供条件 レンチウイルスベクターについて、本件研究材料を利用した研究結果等を発表する際は、レンチウイルスベクターが故三好浩之博士により開発されたことを明示する。
          提供依頼 手続きの詳細は、「レンチウイルスベクターの提供申し込み」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (非営利機関による非営利学術研究用)[Word]
          遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。
          備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。
          提供条件は英文のまま記載してください。
          Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB15442 mCherry-hCdt1(1/100)/pCSII-EF DNA solution

          Ordering instruction of plasmids [in Japanese] [in English]
          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Sakaue-Sawano, A., Genetically Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5 (2017). PMID 29107535.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Fucci-related materials (en) (English text)
          Featured content Fucci-related materials (ja) (Japanese text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB15442_A7Jvp1-3.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: EF1a-C_F
          Region: EF-1 alpha pro,mCherry-hCdt1(1/100) 5'
          Sequence file: RDB15442_A7Jva.seq check
          >D05018A2_A7Jv_2_EF1a-C_F_A01_01_ABI24.ab1
              1 CCTTTGGGAA TTTGGCCCTT TTTGAGTTTG GATCTTGGTT CATTCTCAAG CCTCAGACAG
             61 TGGTTCAAAG TTTTTTTCTT CCATTTCAGG TGTCGTGAAC ACGCTACCGG TCTCGAGAAT
            121 TCGCCACCAT GGTGAGCAAG GGCGAGGAGG ATAACATGGC CATCATCAAG GAGTTCATGC
            181 GCTTCAAGGT GCACATGGAG GGCTCCGTGA ACGGCCACGA GTTCGAGATC GAGGGCGAGG
            241 GCGAGGGCCG CCCCTACGAG GGCACCCAGA CCGCCAAGCT GAAGGTGACC AAGGGTGGCC
            301 CCCTGCCCTT CGCCTGGGAC ATCCTGTCCC CTCAGTTCAT GTACGGCTCC AAGGCCTACG
            361 TGAAGCACCC CGCCGACATC CCCGACTACT TGAAGCTGTC CTTCCCCGAG GGCTTCAAGT
            421 GGGAGCGCGT GATGAACTTC GAGGACGGCG GCGTGGTGAC CGTGACCCAG GACTCCTCCC
            481 TGCAGGACGG CGAGTTCATC TACAAGGTGA AGCTGCGCGG CACCAACTTC CCCTCCGACG
            541 GCCCCGTAAT GCAGAAGAAG ACCATGGGCT GGGAGGCCTC CTCCGAGCGG ATGTACCCCG
            601 AGGACGGCGC CCTGAAGGGC GAGATCAAGC AGAGGCTGAA GCTGAAGGAC GGCGGCCACT
            661 ACGACGCTGA GGTCAAGACC ACCTACAAGG CCAAGAAGCC CGTGCAGCTG CCCGGCGCCT
            721 ACAACGTCAA CATCAAGTTG GACATCACCT CCCACAACGA GGACTACACC ATCGTGGAAC
            781 AGTACGAACG CGCCGAGGGC CGCCACTCCA CCGGCGGCAT GGACGAGCTG TACAAGGGAT
            841 ATCCATCACA CTGGCGGCCG CTCGAGATGG AGCAGCGCCG CGTCACCGAC TTCTTCGCGC
            901 GCCGCCGCCC CGGGCCCCCC CCGCATCGCG CCGCCCAAGC TGGCCTGCCG CACCCCCAGC
            961 CCCGCCAGGC CCGCACTCCG CGCCCCGGCC TCCGCTACCA GTGGCAGCCG CAAGCGCGCC
           1021 CGCCCGGCCG CCGCCCCCGG ACGCGAACCA GGCCAGGGCC ACCGGGCCCG CAGAGACTGC
           1081 GGCTGGTCGG TGGAACGAGT TTCAGCCCCA AGTTACCCCC GAGGCCTCAG AAACATTCCC
          1141 AGGCCTGCCC GTTC
          //
          Primer: PRE_rev
          Region: PRE,mCherry-hCdt1(1/100) 3'
          Sequence file: RDB15442_A7Jvb.seq check
          >D05018A2_A7Jv_2_PRE_rev_B01_04_ABI24.ab1
              1 AGGAATCACA TAGCGTAAAG GAGCAACATA GTTAAGAATA CCAGTCAATC TTTCACAAAT
             61 TTTGTAATCC AGAGGTTGAT TATCGATAAG CTTGATCCCT CGATGTTAAC TCTAGATTAT
            121 TTCTTTATCT TCTGGCCCGG AGAAGGGCAG GCTGGGATGT CTGGGGCCTC GGGGGTACTG
            181 GGGCTGGAAA CCTCGTCCAC CGACAGCCGC AGTCTCCTGC GGGCCGGTGG CCTGGCCTGG
            241 TCGCGTCCGG GGGCGGCGGG CGGGCGGGCG CGCTTGCGGC TGCCACTGGT AGCGGAGGCC
            301 GGGGCGCGGA GTGCGGGCCT GGCGGGGCTG GGGGTGCGGC AGGCCAGCTT GGGCGGCGCG
            361 ATGCGGGGGG GCCCGGGGCG GCGGCGCGCG AAGAAGTCGG TGACGCGGCG CTGCTCCATC
            421 TCGAGCGGCC GCCAGTGTGA TGGATATCCC TTGTACAGCT CGTCCATGCC GCCGGTGGAG
            481 TGGCGGCCCT CGGCGCGTTC GTACTGTTCC ACGATGGTGT AGTCCTCGTT GTGGGAGGTG
            541 ATGTCCAACT TGATGTTGAC GTTGTAGGCG CCGGGCAGCT GCACGGGCTT CTTGGCCTTG
            601 TAGGTGGTCT TGACCTCAGC GTCGTAGTGG CCGCCGTCCT TCAGCTTCAG CCTCTGCTTG
            661 ATCTCGCCCT TCAGGGCGCC GTCCTCGGGG TACATCCGCT CGGAGGAGGC CTCCCAGCCC
            721 ATGGTCTTCT TCTGCATTAC GGGGCCGTCG GAGGGGAAGT TGGTGCCGCG CAGCTTCACC
            781 TTGTAGATGA ACTCGCCGTC CTGCAGGGAG GAGTCCTGGG TCACGGTCAC CACGCCGCCG
            841 TCCTCGAAGT TCATCACGCG CTCCCACTTG AAGCCCTCGG GGAAGGACAG CTTCAAGTAG
            901 TCGGGGATGT CGGCGGGGTG CTTCACGTAG GCCCTTGGAG CCGTACATGA ACTGAGGGGA
            961 CAGGGATGTC CCAGGCGAAG GCAGGGGCCA CCCTTTGGTC ATCATCAGCT GCGGTCTGGT
           1021 GCCTTCGGTA GGGCGTCTCG TCTCGCCATC GATCTCGACT CGTGTCGGTC ACGAGTCATC
          1081 ATGTGCACAT GGAGCGCAAT GGACCTTCCA TTGAG
          //
          Primer: CMV_Forward
          Region: CMV pro,5'LTR,HIV-1 psi
          Sequence file: RDB15442_A7Jvc.seq check
          >D05018A2_A7Jv_2_CMV-Forward_C01_07_ABI24.ab1
              1 CGGTTCCACC TCCGGCCCCA TTGACGCAAA TGGGCGGTAG GCGTGTACGG TGGGAGGTCT
             61 ATATAAGCAG CGCGTTTTGC CTGTACTGGG TCTCTCTGGT TAGACCAGAT CTGAGCCTGG
            121 GAGCTCTCTG GCTAACTAGG GAACCCACTG CTTAAGCCTC AATAAAGCTT GCCTTGAGTG
            181 CTTCAAGTAG TGTGTGCCCG TCTGTTGTGT GACTCTGGTA ACTAGAGATC CCTCAGACCC
            241 TTTTAGTCAG TGTGGAAAAT CTCTAGCAGT GGCGCCCGAA CAGGGACTTG AAAGCGAAAG
            301 GGAAACCAGA GGAGCTCTCT CGACGCAGGA CTCGGCTTGC TGAAGCGCGC ACGGCAAGAG
            361 GCGAGGGGCG GCGACTGGTG AGTACGCCAA AAATTTTGAC TAGCGGAGGC TAGAAGGAGA
            421 GAGATGGGTG CGAGAGCGTC AGTATTAAGC GGGGGAGAAT TAGATCGCGA TGGGAAAAAA
            481 TTCGGTTAAG GCCAGGGGGA AAGAAAAAAT ATAAATTAAA ACATATAGTA TGGGCAAGCA
            541 GGGAGCTAGA ACGATTCGCA GTTAATCCTG GCCTGTTAGA AACATCAGAA GGCTGTAGAC
            601 AAATACTGGG ACAGCTACAA CCATCCCTTC AGACAGGATC AGAAGAACTT AGATCATTAT
            661 ATAATACAGT AGCAACCCTC TATTGTGTGC ATCAAAGGAT AGAGATAAAA GACACCAAGG
            721 AAGCTTTAGA CAAGATAGAG GAAGAGCAAA ACAAAAGTAA GACCACCGCA CAGCAAGGGC
            781 CGCTGATCTT CAGACCTGGA GGAGGAGATA TGAGGGACAA TTGGAGAAGT GAATTATATA
            841 AATATAAAGT AGTAAAAATT GAACCATTAG GAGTAGCACC CACCAAGGCA AAGAGAAGAG
            901 TGGTGCAGAG AGAAAAAAAG AGCAGTGGGA ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA
            961 GCAGCAGGAA GCACTATGGG CGCAGCGTCA ATGACGCTGA CGGTACAGGC CCAGACAATT
           1021 ATTGTCTGGG TATAGTGCAG CAGCCAGACA ATTTGCTGGA GGCCTATTGA GCGCAACCAG
          1081 CATCTGTGCA CTCACAGTCC TGGGGGCATC AAGTAAGCCT CCCAAG
          //
          Primer: EF1a-N_R
          Region: EF-1 alpha pro,cPPT_CTS,RRE
          Sequence file: RDB15442_A7Jvf.seq check
          >15442_15442_A9Da_EF1a-N_R_C01_03_ABI08.ab1
              1 TCCTTTTCCC GGGGGATTGT GGGCGATGTG CGCTCTGCCC ACTGACGGGC ACCGGAGCCT
             61 CACGAAGCTT GAATTAATTC AAAGTGAATT TCTGCTGTCC CTGTAATAAA CCCGAAAATT
            121 TTGAATTTTT GTAATTTGTT TTTGTAATTC TTTAGTTTGT ATGTCTGTTG CTATTATGTC
            181 TACTATTCTT TCCCCTGCAC TGTACCCCCC AATCCCCCCT TTTCTTTTAA AATTGTGGAT
            241 GAATACTGCC ATTTGTAGAT CCGTTCACTA ATCGAATGGA TCTGTCTCTG TCTCTCTCTC
            301 CACCTTCTTC TTCTATTCCT TCGGGCCTGT CGGGTCCCCT CGGGGTTGGG AGGTGGGTCT
            361 GAAACGATAA TGGTGAATAT CCCTGCCTAA CTCTATTCAC TATAGAAAGT ACAGCAAAAA
            421 CTATTCTTAA ACCTACCAAG CCTCCTACTA TCATTATGAA TAATTTTATA TACCACAGCC
            481 AATTTGTTAT GTTAAACCAA TTCCACAAAC TTGCCCATTT ATCTAATTCC AATAATTCTT
            541 GTTCATTCTT TTCTTGCTGG TTTTGCGATT CTTCAATTAA GGAGTGTATT AAGCTTGTGT
            601 AATTGTTAAT TTCTCTGTCC CACTCCATCC AGGTCGTGTG ATTCCAAATC TGTTCCAGAG
            661 ATTTATTACT CCAACTAGCA TTCCAAGGCA CAGCAGTGGT GCAAATGAGT TTTCCAGAGC
            721 AACCCCAAAT CCCCAGGGAG CTGTTGATCC TTTAGGTATC TTTCCACAGC CAGGATTCTT
            781 GCCTGGAGCT GCTTGATGCC CCAGACTGTG AGTTGCAACA GATGCTGTTG CGCCTCAATA
            841 GCCCTCAGCA AATTGTTCTG CTGCTGCACT ATACCAGACA ATAAATTGTC TGGCCTGTAC
            901 CGTCAGCGTC ATTGACGCTG CGCCCATAGT GCTTCCTGCT GCTCCCAAGA ACCCAAAGGA
            961 AACAAAGCTC CTATTCCCAC TGCTCCTTTT TTTCTCTCTG CACCACTCTT TCTCTTTGCT
           1021 TTGGGTGGGT GCTACTTCCT AAATGGTTCG TTTTTACTAC TTTATATTTA TATAAATCAA
           1081 CCTTCTTCCA AATGGTCCGC TCAAATCTAC TCTCAAGGTT CCTGGAAGAA ATTCCAAGAC
          1141 CGGCG
          //
          Primer: BGH_rev2
          Region: bGHpA,3' LTR,PRE
          Sequence file: RDB15442_A7Jvd.seq check
          >D05018A2_A7Jv_2_BGH_rev2_D01_10_ABI24.ab1
              1 CATTCAGGTT AGATGCACGG GGGAGGGGCA AACAACAGAT GGCTGGCAAC TAGAAGGCAC
             61 AGTCGAGGCT GATCAGCGGG TTTAAACGGG CCCTGCTAGA GATTTTCCAC ACTGACTAAA
            121 AGGGTCTGAG GGATCTCTAG TTACCAGAGT CACACAACAG ACGGGCACAC ACTACTTGAA
            181 GCACTCAAGG CAAGCTTTAT TGAGGCTTAA GCAGTGGGTT CCCTAGTTAG CCAGAGAGCT
            241 CCCAGGCTCA GATCTGGTCT AACCAGAGAG ACCCAGTACA GTCCGGATGC AGCTCTCGGG
            301 CCATGTGATG AAATGCTAGG CGGCTGTCAA ACCTCCACTC TAATACTTCT CTCTCCGGGT
            361 CATCCATCCC ATGCAGGCTC ACAGGGTGTA ACAAGCGGGT GTTCTCTCCT TCATTGGCTT
            421 CTTCTACCTT CTCTTGCTCA ACTGGTACTA GCTTGTAGCA CCATCCAAAG GTCAGTGGAT
            481 ATCTGATCCC TGGCCCTGGT GTGTAGTTCT GCCAATCAGG GAAGTAGCCT TGTGTGTGGT
            541 AGATCCACAG ATCAAGGATA TCTTGTCTTC GTTGGGAGTG AATTAGCCCT TCCAGTCCCC
            601 CCTTTTCTTT TAAAAAGTGG CTAAGATCTA CAGCTGCCTT GTAAGTCATT GGTCTTAAAG
            661 GTACCTGAGG TGTGACTGGA AAACCCACCT CCTCCTCCTC TTGTGCTTCT AGCCAGGCAC
            721 AATCAGCATT GGTAGCTGCT GTATTGCTAC TTGTGATTGC TCCATGTTTT TCTAGGTCTC
            781 GACGGTATCG ATGCGGGGAG GCGGCCCAAA GGGAGATCCG ACTCGTCTGA GGGCGAAGGC
            841 GAAGACGCGG AAGAGGCCGC AGAGCCGGCA GCAGGCCGCG GGAAGGAAAG GTCCGCTGGA
            901 TTGAGGGCCG AAAGGGACGT AGCAGAAGGA CGTCCCGCGC AGAAATCCAG GTGGCAACAC
            961 AGGCGAGCAG CCCATGGAAA AGGACGTCAG CTTCCCCGAC ACACCACGGG AAATTGTCAG
           1021 TGCCCACAGC CGAGCTCCTG TCCAGCAGCG GGCAGCAGCG GCGGATGAGG TCGTCGTGCC
           1081 ATAGGGAGGG GGAAGCGAGT CTGGCGGAGC TGACAGTGTG GCCAATGCCT CAACCAAGTG
          1141 GGGGGAATC
          //
          Primer: SV40pro_F
          Region: SV40 pro_ori,Zeo,SV40pA
          Sequence file: RDB15442_A7Jve.seq check
          >D05018A2_A7Jv_2_SV40pro_F_E01_13_ABI24.ab1
              1 CCCTGGGCCC TTGACATTCA GAGTAGTGAG GAGGCTTTTT TGGAGGCCTA GGCTTTTGCA
             61 AAAAGCTCCC GGGAGCTTGT ATATCCATTT TCGGATCTGA TCAGCACGTG TTGACAATTA
            121 ATCATCGGCA TAGTATATCG GCATAGTATA ATACGACAAG GTGAGGAACT AAACCATGGC
            181 CAAGTTGACC AGTGCCGTTC CGGTGCTCAC CGCGCGCGAC GTCGCCGGAG CGGTCGAGTT
            241 CTGGACCGAC CGGCTCGGGT TCTCCCGGGA CTTCGTGGAG GACGACTTCG CCGGTGTGGT
            301 CCGGGACGAC GTGACCCTGT TCATCAGCGC GGTCCAGGAC CAGGTGGTGC CGGACAACAC
            361 CCTGGCCTGG GTGTGGGTGC GCGGCCTGGA CGAGCTGTAC GCCGAGTGGT CGGAGGTCGT
            421 GTCCACGAAC TTCCGGGACG CCTCCGGGCC GGCCATGACC GAGATCGGCG AGCAGCCGTG
            481 GGGGCGGGAG TTCGCCCTGC GCGACCCGGC CGGCAACTGC GTGCACTTCG TGGCCGAGGA
            541 GCAGGACTGA CACGTGCTAC GAGATTTCGA TTCCACCGCC GCCTTCTATG AAAGGTTGGG
            601 CTTCGGAATC GTTTTCCGGG ACGCCGGCTG GATGATCCTC CAGCGCGGGG ATCTCATGCT
            661 GGAGTTCTTC GCCCACCCCA ACTTGTTTAT TGCAGCTTAT AATGGTTACA AATAAAGCAA
            721 TAGCATCACA AATTTCACAA ATAAAGCATT TTTTTCACTG CATTCTAGTT GTGGTTTGTC
            781 CAAACTCATC AATGTATCTT ATCATGTCTG TATACCGTCG ACCTCTAGCT AGAGCTTGGC
            841 GTAAATCATG GTCATAGCTG TTTCCTGTGT GAAATTGTTA TCCGCTCAAC AATTCCACAC
            901 AACATACGAG CCGGGAAGCA TAAAGTGTAA AGCCCTGGGG TGCCTAATGA GTGAGCTAAC
            961 TCACATTAAT TGCGGTTGCG CTCACTGCCC GGCTTTCCAG TCGGGGAACC CTGTCGTGCC
           1021 AGCTGCATTA ATGGATCGGG CCAACGCGCG GGGGAGAGGC GGTTTTGCGT ATTTGGGCGC
           1081 TCTTCCGACT TCCTTCGCTC AACTTGAACT CGGCTGGCGC CTCGGATCGA TCAGGCTGGC
          1141 GGCGGGCAGG ATAATCAAGA CGTTCACAAT
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Sakaue-Sawano, A., Genetically Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5 (2017). PMID 29107535.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl