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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

mCherry-hCdt1(30/120)/pcDNA3

Expression vector of Fucci2-G1 phase probe. One of the component of Fucci2 cell cycle probe.

Catalog number RDB15457
Resource name mCherry-hCdt1(30/120)/pcDNA3
Clone info. Expression vector of Fucci2-G1 phase probe. One of the component of Fucci2 cell cycle probe. Fluorescent was detected in the nucleus.
Vector backbone pcDNA3 (plasmid)
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells)
Gene/insert name Human CDT1 cDNA Discosoma sp. RFP cDNA
Reference of insert sequence AB512478 (DDBJ accession number)
Depositor|Developer Miyawaki, Atsushi |
Other clones in our bank

External Database
Human CDT1 Discosoma sp. RFP

          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution 1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
          2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
          Sakaue-Sawano A, Kobayashi T, Ohtawa K, Miyawaki A. (2011) Drug-induced cell cycle modulation leading to cell-cycle arrest, nuclear mis-segregation, or endoreplication. BMC Cell Biology, 12:2.
          3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
          4. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
          5. The RECIPIENT shall obtain written approval from the UCSD using the MTA provided by the Technology Transfer office of UCSD.
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          MTA, for use for not-for-profit academic purpose [Word]
          Please visit Information of Request for Distribution.[link] 
          Remarks
          This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.
          提供案内 (日本国内) [open/close]

          提供条件 1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
          2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
          Sakaue-Sawano A, Kobayashi T, Ohtawa K, Miyawaki A. (2011) Drug-induced cell cycle modulation leading to cell-cycle arrest, nuclear mis-segregation, or endoreplication. BMC Cell Biology, 12:2.
          3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
          4. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
          5. The RECIPIENT shall obtain written approval from the UCSD using the MTA provided by the Technology Transfer office of UCSD.
          提供依頼
          提供依頼書 [Word]
          提供同意書 (MTA, 非営利学術目的用)[Word]
          手続きの概要は、「提供申込みについて[link]」をご覧ください。
          備考 提供条件は英文のまま記載してください。
          Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB15457 mCherry-hCdt1(30/120)/pcDNA3 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The mCherry-hCdt1(30/120)/pcDNA3 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15457).

          Reference section:

          Sakaue-Sawano, A., Kobayashi, T., Ohtawa, K., Miyawaki, A., Drug-induced cell cycle modulation leading to cell-cycle arrest, nuclear mis-segregation, or endoreplication. BMC Cell Biol. 12: 2 (2011). PMID 21226962. [link to RRC of NBRP]

          Further references such as user reports and related articles (go to bottom)


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          Featured content

          Featured content Fucci-related materials (en) (English text)
          Featured content Fucci-related materials (ja) (Japanese text)

          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB15457_A7Jvp1-4.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV_Forward (Pr0016)
          Region: CMV pro,insert 5'
          Sequence file: RDB15457_A7Jvd.seq check
          >15457_15457_B3E2F_CMV_Forward_H01_08_ABI08.ab1
              1 NNNNNNNNNN NNNCCCNNTT GNNGCAANNG GGCGGTAGGC GTGTACGGTG GGAGGTCTAT
             61 ATAAGCAGAG CTCTCTGGCT AACTAGAGAA CCCACTGCTT ACTGGCTTAT CGAAATTAAT
            121 ACGACTCACT ATAGGGAGAC CCAAGCTTGG TACCGAGCTC GGATCCACTA GTAACGGCCG
            181 CCAGTGTGCT GGAATTCGCC ACCATGGTGA GCAAGGGCGA GGAGGATAAC ATGGCCATCA
            241 TCAAGGAGTT CATGCGCTTC AAGGTGCACA TGGAGGGCTC CGTGAACGGC CACGAGTTCG
            301 AGATCGAGGG CGAGGGCGAG GGCCGCCCCT ACGAGGGCAC CCAGACCGCC AAGCTGAAGG
            361 TGACCAAGGG TGGCCCCCTG CCCTTCGCCT GGGACATCCT GTCCCCTCAG TTCATGTACG
            421 GCTCCAAGGC CTACGTGAAG CACCCCGCCG ACATCCCCGA CTACTTGAAG CTGTCCTTCC
            481 CCGAGGGCTT CAAGTGGGAG CGCGTGATGA ACTTCGAGGA CGGCGGCGTG GTGACCGTGA
            541 CCCAGGACTC CTCCCTGCAG GACGGCGAGT TCATCTACAA GGTGAAGCTG CGCGGCACCA
            601 ACTTCCCCTC CGACGGCCCC GTAATGCAGA AGAAGACCAT GGGCTGGGAG GCCTCCTCCG
            661 AGCGGATGTA CCCCGAGGAC GGCGCCCTGA ANGGCGAGAT CAAGCAGANG CTGAAGCTGA
            721 ANGACGGCGG CCACTACGAC GCTGANGTCA AGANCACCTA CAAGGCCNAG AAGCCCGTGC
            781 AGCTGCCCGG CGCCTACNAC GTCNACATCA AGTTGGACNT CACCTCNNAC AACGANGNNT
            841 ACACCATCNN NNNANNAGTA CGAACGCNNN NANGGNCGNN NCTCCNCCGG CGGNNTGGNN
            901 GANCTGTNNN AGGGATATNN NTCACACTGG CNGCNGNNNN NNCCCANNNN CNNNNNGNNC
          961 NNNANTNNNN NNNNNN
          //
          Primer: BGH_rev2 (Pr0606)
          Region: insert 3'
          Sequence file: RDB15457_A7Jve.seq check
          >D05018B8-2_A7Jv_BGH_rev2_D03_12_ABI24.ab1
              1 NNNNNNNNNN GNNNNNNGGG GGNNGGNNNN NNACAGATGG CTGGCAACTA GAAGGCACAG
             61 TCGAGGCTGA TCANNNNGCT CTAGCATTTA GGTGACACTA TAGAATAGGG CCCTCTAGAT
            121 TAGATGGTGT CCTGGTCCTG CGCGGATGTC AGGTGGGGCG GCTGACCTGC TGCCGGGGTG
            181 GATTTCTTTA TCTTCTGGCC CGGAGAAGGG CAGGCTGGGA TGTCTGGGGC CTCGGGGGTA
            241 CTGGGGCTGG AAACCTCGTC CACCGACAGC CGCAGTCTCC TGCGGGCCGG TGGCCTGGCC
            301 TGGTCGCGTC CGGGGGCGGC GGGCGGGCGG GCGCGCTTGC GGCTGCCACT GGTAGCGGAG
            361 GCCGGGGCGC GGAGTGCGGG CCTGGCGGGG CTGGGCTCGA GCGGCCGCCA GTGTGATGGA
            421 TATCCCTTGT ACAGCTCGTC CATGCCGCCG GTGGAGTGGC GGCCCTCGGC GCGTTCGTAC
            481 TGTTCCACGA TGGTGTAGTC CTCGTTGTGG GAGGTGATGT CCAACTTGAT GTTGACGTTG
            541 TAGGCGCCGG GCAGCTGCAC GGGCTTCTTG GCCTTGTAGG TGGTCTTGAC CTCAGCGTCG
            601 TAGTGGCCGC CGTCCTTCAG CTTCAGCCTC TGCTTGATCT CGCCCTTCAG GGCGCCGTCC
            661 TCGGGGTACA TCCGCTCGGA NGANGCCTCC CAGCCCATGG TCTTCTTCTG CATTACGGGG
            721 CCGTCGGANG GGAAGTTGGT GCCGCGCAGC TTCACCTTGT AGATGAACTC GCCGTCCTGC
            781 ANGGANGANT CCTGGGTCAC GGTCACCACG CCGCCGTCCT CGAAGTNCAT CACGCGCNNN
            841 NCTTGAAGCC CTCNGGGAAN GANAGCTTCA AGTANTCNGG GNNGTCGGCG GGGTGCTNNA
            901 CGNNNNNCTN NGAGCNNNNC NTGAANNNNN GGGNNAGGNN GNNCNNNNNN NANGGGNNNN
          961 GGNNNNNCNN NNGNNNNNNN TNNNNCNNNN NNGNNNNGNN NNNNNNNN
          //
          Primer: SV40pro_F (Pr0110)
          Region: NeoR
          Sequence file: RDB15457_A7Jvf.seq check
          >D05018B8_2_A7Jv_SV40pro_F_C07_07_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNA NNNNTGNNGN NGCTTTTTTG GANGCCTAGG CTTTTGCAAA
             61 AAGCTCCCGG GNGCTTGTAT ATCCATTTTC GGATCTGATC AAGAGACAGG ATGAGGATCG
            121 TTTCGCATGA TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG
            181 CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG
            241 CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT
            301 GAACTGCAGG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA
            361 GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG
            421 GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT
            481 GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA
            541 CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG
            601 GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGCGCATG
            661 CCCGACGGCG ANGATCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG
            721 GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGNNCGGC TGGGTGTGGC GGANCGCTAT
            781 CANGACATAG CGTTGGCTAC NCGTGATATT GCTGAAGAGC TTGGCGGCGA ATGGGCTGAC
            841 NGCTTCCNCG TGCTTTACGG NATCGCCGCT CCCNANNCGC AGCGCATCGC CTTCTATCGC
            901 CTTCTTGACG ANTTCTTCTG ANCGGGNNNC TGGGGGTTCN NAATGANNNA NNNANCGACG
            961 CCNNANNNGN NNNCNCNANN NTNNNANTNC NNNNNNCNNN CNNNNNNNNN ANNNNNNGGN
          1021 NNTNNNNNNN NNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Sakaue-Sawano, A., Drug-induced cell cycle modulation leading to cell-cycle arrest, nuclear mis-segregation, or endoreplication. BMC Cell Biol. 12: 2 (2011). PMID 21226962. [link to RRC of NBRP]

          2024.03.26

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