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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

mVenus-hGeminin(1/110)/pcDNA3

Expression vector of Fucci2-S/G2/M phase probe. Component of Fucci2 cell cycle probe.

Catalog number RDB15458
Resource name mVenus-hGeminin(1/110)/pcDNA3
Clone info. Expression vector of Fucci2-S/G2/M phase probe, S/G2/M-Y(N). One of the component of Fucci2 cell cycle probe. Fluorescent was detected in the nucleus.
For the composition, refer Fig.1 of Sakaue-Sawano, A., Chem. Biol. 15 (12): 1243-1248 (2008).
Vector backbone pcDNA3 (plasmid)
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells)
Gene/insert name Human Geminin cDNA Aequorea victoria GFP cDNA
Reference of insert sequence AB512479 (DDBJ accession number)
Depositor|Developer Miyawaki, Atsushi |
Other clones in our bank

External Database
Human Geminin Aequorea victoria GFP

          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution 1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
          2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
          Sakaue-Sawano A, Ohtawa K, Hama H, Kawano M, Ogawa M, Miyawaki A. (2008) Tracing the Silhouette of Individual Cells in S/G2/M Phases with Fluorescence. Chemistry & Biology, 15: 1243-1248.
          Sakaue-Sawano A, Kobayashi T, Ohtawa K, Miyawaki A. (2011) Drug-induced cell cycle modulation leading to cell-cycle arrest, nuclear mis-segregation, or endoreplication. BMC Cell Biology, 12:2.
          3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
          4. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
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          MTA, for use for not-for-profit academic purpose [Word]
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          Please visit Information of Request for Distribution.[link] 
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          提供条件 1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
          2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
          Sakaue-Sawano A, Ohtawa K, Hama H, Kawano M, Ogawa M, Miyawaki A. (2008) Tracing the Silhouette of Individual Cells in S/G2/M Phases with Fluorescence. Chemistry & Biology, 15: 1243-1248.
          Sakaue-Sawano A, Kobayashi T, Ohtawa K, Miyawaki A. (2011) Drug-induced cell cycle modulation leading to cell-cycle arrest, nuclear mis-segregation, or endoreplication. BMC Cell Biology, 12:2.
          3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
          4. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
          提供依頼
          提供依頼書 [Word]
          提供同意書 (MTA, 非営利学術目的用)[Word]
          提供同意書 (MTA, 営利目的用)[Word]
          手続きの概要は、「提供申込みについて[link]」をご覧ください。
          備考 提供条件は英文のまま記載してください。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB15458 mVenus-hGeminin(1/110)/pcDNA3 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The mVenus-hGeminin(1/110)/pcDNA3 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15458).

          Reference section:

          Sakaue-Sawano, A., Kobayashi, T., Ohtawa, K., Miyawaki, A., Drug-induced cell cycle modulation leading to cell-cycle arrest, nuclear mis-segregation, or endoreplication. BMC Cell Biol. 12: 2 (2011). PMID 21226962. [link to RRC of NBRP]
          Sakaue-Sawano, A., Ohtawa, K., Hama, H., Kawano, M., Ogawa, M., Miyawaki, A., Tracing the silhouette of individual cells in S/G2/M phases with fluorescence. Chem. Biol. 15 (12): 1243-1248 (2008). PMID 19101468. [link to RRC of NBRP]

          Further references such as user reports and related articles (go to bottom)


          Tips

          Featured content

          Featured content Fucci-related materials (en) (English text)
          Featured content Fucci-related materials (ja) (Japanese text)

          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB15458_A7Jvp1-5.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV_Forward (Pr0016)
          Region: MCS,insert 5'
          Sequence file: RDB15458_A7Jvd.seq check
          >D05018B9-2_A7Jv_CMV-Forward_E03_15_ABI24.ab1
              1 NNNNNNNNNN NNNNNNCNNT TGNNGCNNNG GGCGGTAGGC GTGTACGGTG GGAGGTCTAT
             61 ATAAGCAGAG CTNNNNNNNN NNNNNNAGAA CCCACTGCTT ACTGGCTTAT CGAAATTAAN
            121 ACNACTCACT ATAGGGAGAC CCAAGCTTGG TACCGAGCTC GGATCCACTA GTAACGGCCG
            181 CCAGTGTGCT GGAATTCATG GTGAGCAAGG GCGAGGAGCT GTTCACCGGG GTGGTGCCCA
            241 TCCTGGTCGA GCTGGACGGC GACGTAAACG GCCACAAGTT CAGCGTGTCC GGCGAGGGCG
            301 AGGGCGATGC CACCTACGGC AAGCTGACCC TGAAGCTGAT CTGCACCACC GGCAAGCTGC
            361 CCGTGCCCTG GCCCACCCTC GTGACCACCC TGGGCTACGG CCTGCAGTGC TTCGCCCGCT
            421 ACCCCGACCA CATGAAGCAG CACGACTTCT TCAAGTCCGC CATGCCCGAA GGCTACGTCC
            481 AGGAGCGCAC CATCTTCTTC AAGGACGACG GCAACTACAA GACCCGCGCC GAGGTGAAGT
            541 TCGAGGGCGA CACCCTGGTG AACCGCATCG AGCTGAAGGG CATCGACTTC AAGGAGGACG
            601 GCAACATCCT GGGGCACAAG CTGGAGTACA ACTACAACAG CCACAACGTC TATATCACCG
            661 CCGACAAGCA GAAGAACGGC ATCAAGGCCA ACTTCAAGAT CCGCCACAAC ATCGAGGANG
            721 GCGGCGTGCA GCTCGCCGAC CACTACCAGC AGAACACCCC CATCGGCGAC GGCCCCGTGC
            781 TGCTGCCCGA CAACCACTAC CTGANCTACC AGTCCNAGCT GAGCAAAGAC CCCAACGANA
            841 AGCGCGATCA CATGGTCCNG CTGNAGTTCG TGACCGCCGC CNGGATCACT CTCGGCNNGG
            901 ACGANCTGTA CAAGGGATAT CCNTCACACN NGCGGNCNNC NCNNNATGAA TNCNNGTNTG
            961 AANCAGAANN NNNNANGNNN NNAAGANNNN ATAANNANTA GTTNNGNNCC NNGNNNNNNN
          1021 NNN
          //
          Primer: BGH_rev2 (Pr0606)
          Region: insert 3'
          Sequence file: RDB15458_A7Jve.seq check
          >D05018B9-2_A7Jv_BGH_rev2_F03_18_ABI24.ab1
              1 NNNNNNNNNN NNGNNNNNNG GGGGNNGGGC AACAACAGAT GGCTGGCAAC TAGAAGGCAC
             61 AGTCGAGGCT GANNNNNNNN CTCTAGCATT TAGGTGACAC TATAGAATAG GGCCCTCTAG
            121 ATTACAGCGC CTTTCTCCGT TTTTCTGCCA CTTCCTTCCA ATACTGAGAG GATGGATTTT
            181 CTTTAATCAT AAGATCAAAT GACTCCTGGG TGACTCCTCC AAGATTTTTA TTTTCACTAG
            241 ATTCTGGGAC AATAACCCCA GGGCTGGAAG TTGTAGATGT TAAGTGGTCA TTCCGATGTT
            301 TCCTTTTGGA CAAGCCTGCG GACAGCTCAT TTTCTCTTCC AACAAGAGAT CCAGATGCAG
            361 AAGGCTGAAT CATCTTCAGA GTTCTTCTTG GGACAGAACT ATTCTTTATA TTCTCTTTGA
            421 TTTCTTCTTG TTTCTGCTTC ATACTGGGAT TCATCTCGAG CGGCCGCCAG TGTGATGGAT
            481 ATCCCTTGTA CAGCTCGTCC ATGCCGAGAG TGATCCCGGC GGCGGTCACG AACTCCAGCA
            541 GGACCATGTG ATCGCGCTTC TCGTTGGGGT CTTTGCTCAG CTTGGACTGG TAGCTCAGGT
            601 AGTGGTTGTC GGGCAGCAGC ACGGGGCCGT CGCCGATGGG GGTGTTCTGC TGGTAGTGGT
            661 CGGCGAGCTG CACGCCGCCG TCCTCGATGT TGTGGCGGAT CTTGAAGTTG GCCTTGATGC
            721 CGTTCTTCTG CTTGTCGGCG GTGNTATAGA CGTTGTGGCT GTTGTAGTTG TACTCCAGCT
            781 TGTGCCCCAG GATGTTGCCG TCCTCCTTGA AGTCGATGCC NTTCAGCTCG ATGCGGTTCA
            841 CCAGGGTGTC GCCCTCNNAC TTCACCTCGG CGCGGGTCTN GTANTTGCCG NCGTCCNNGA
            901 AGAAGANGGT GCGCTCCTGN ANNNANCCTT CGGGCATGGC GGANTTNNAG AANNCGTGCT
          961 GCTTCATNTG GNCNGGGNAN CGGGCGNNNC NCNNCNNNNN NNNNN
          //
          Primer: SV40pro_F (Pr0110)
          Region: SV40 pro_ori,NeoR
          Sequence file: RDB15458_A7Jvf.seq check
          >D05018B9_2_A7Jv_SV40pro_F_B07_04_ABI24.ab1
              1 NNNNNNNNNN NNNNNNANNN GTGNNGNNGC TTTTTTGGAG GCCTAGGCTT TTGCAAAAAG
             61 CTCCCGGGAG CTTGTATATC CATTTTCGGA TCTGATCAAG AGACAGGATG AGGATCGTTT
            121 CGCATGATTG AACAAGATGG ATTGCACGCA GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA
            181 TTCGGCTATG ACTGGGCACA ACAGACAATC GGCTGCTCTG ATGCCGCCGT GTTCCGGCTG
            241 TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC AAGACCGACC TGTCCGGTGC CCTGAATGAA
            301 CTGCAGGACG AGGCAGCGCG GCTATCGTGG CTGGCCACGA CGGGCGTTCC TTGCGCAGCT
            361 GTGCTCGACG TTGTCACTGA AGCGGGAAGG GACTGGCTGC TATTGGGCGA AGTGCCGGGG
            421 CAGGATCTCC TGTCATCTCA CCTTGCTCCT GCCGAGAAAG TATCCATCAT GGCTGATGCA
            481 ATGCGGCGGC TGCATACGCT TGATCCGGCT ACCTGCCCAT TCGACCACCA AGCGAAACAT
            541 CGCATCGAGC GAGCACGTAC TCGGATGGAA GCCGGTCTTG TCGATCAGGA TGATCTGGAC
            601 GAAGAGCATC AGGGGCTCGC GCCAGCCGAA CTGTTCGCCA GGCTCAAGGC GCGCATGCCC
            661 GACGGCGAGG ATCTCGTCGT GACCCATGGC GATGCCTGCT TGCCGAATAT CATGGTGGAA
            721 AATGGCCGCT TTTCTGGANT CATCGACTGT GGNCGGCTGG GTGTGGCGGA NCGCTATCAG
            781 GACATAGCGT TGGCTANNCG TGATATTGCT GAAGAGCTTG GCGGCGAATG NNCTGANCGC
            841 TTCCTCGTGC TTTACGGTAT CGCCGCTCCC GATNCGCAGC GCATCGCCTT CTATCGCCNT
            901 CTTGANGANT TCTTCTGANC GGGANNNNGG GGGTTCNAAN NGNNNNANNN ANCGACGCCN
          961 NNCNNNNNNT CNCNANNNTT CGATTNNNNN NNNNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Sakaue-Sawano, A., Drug-induced cell cycle modulation leading to cell-cycle arrest, nuclear mis-segregation, or endoreplication. BMC Cell Biol. 12: 2 (2011). PMID 21226962. [link to RRC of NBRP]
          original Sakaue-Sawano, A., Tracing the silhouette of individual cells in S/G2/M phases with fluorescence. Chem. Biol. 15 (12): 1243-1248 (2008). PMID 19101468. [link to RRC of NBRP]

          2024.03.26

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