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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pCGT-PDGFRA-C

Expression vector of human PDGFRA (deletion mutant with only the C-terminal portion), T7-tag.

Catalog number RDB15465
Resource name pCGT-PDGFRA-C
Clone info. Expression vector of human PDGFRA (deletion mutant with only the C-terminal portion), tagged with T7 at N-terminal.
Comment Expression was confirmed by the depositor with immunostaining.
Vector backbone pCGT (plasmid)
Selectable markers Amp^r
Gene/insert name human PDGFRA cDNA
Depositor|Developer Kondo, Takeshi |
Other clones in our bank

External Database
human PDGFRA

          Reference sequence
            
           
          Sequence (full) RDB15465zzt01.seq compiled by us

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Ibata, M et al. Cancer Sci. 108 (2), 200-207, 2017). The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose.
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Ibata, M et al. Cancer Sci. 108 (2), 200-207, 2017)。本件研究材料は、非営利機関の非営利学術研究に限って提供する。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

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          RDB15465 pCGT-PDGFRA-C DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The pCGT-PDGFRA-C was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15465).

          Reference section:

          Ibata, M., Iwasaki, J., Fujioka, Y., Nakagawa, K., Darmanin, S., Onozawa, M., Hashimoto, D., Ohba, Y., Hatakeyama, S., Teshima, T., Kondo, T., Leukemogenic kinase FIP1L1-PDGFRA and a small ubiquitin-like modifier E3 ligase, PIAS1, form a positive cross-talk through their enzymatic activities. Cancer Sci. 108 (2): 200-207 (2017). PMID 27960034.

          Further references such as user reports and related articles (go to bottom)


          References and tips

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          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB15465_B2Cgp1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV_Forward (Pr0016)
          Region: CMV pro, HSV TK 5'-UTR, insert (FL) 5'
          Sequence file: RDB15465_B2Cga.seq check
          >D04855B3_B2Cg_1_CMV_Forward_A11_02_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNCCN NNNNNNNNNN ATGGGCGGTA GGCGTGTACG GTGGGAGGTC
             61 TATATAAGCA GAGCTCGTTT AGTGAACCGT CAGATCGCCT GGAGACGCCA TCCACGCTGT
            121 TTTGACCTCC ATAGAAGACA CCGGGACCGA TCCAGCCTCC GGGGGATCTT GGTGGCGTGA
            181 AACTCCCGCA CCTCTTCGGC CAGCGCCTTG TAGAAGCGCG TATGGCTTCT AGGATGGCAT
            241 CGATGACAGG TGGCCAACAG ATGGGTTCTA GGATGGCATC GATGACAGGT GGCCAACAGA
            301 TGGGTTCTAG AGGATCTACT AGTCATATGG ATTGGTCTAG ACTGCCATAT GACTCAAGAT
            361 GGGAGTTTCC AAGAGATGGA CTAGTGCTTG GTCGGGTCTT GGGGTCTGGA GCGTTTGGGA
            421 AGGTGGTTGA AGGAACAGCC TATGGATTAA GCCGGTCCCA ACCTGTCATG AAAGTTGCAG
            481 TGAAGATGCT AAAACCCACG GCCAGATCCA GTGAAAAACA AGCTCTCATG TCTGAACTGA
            541 GGATAATGAC TCACCTGGGG CCACATTTGA ACATCGTAAA CTTGCTGGGA GCCTGCACCA
            601 AGTCAGGCCC CATTTACATC ATCACAGAGT ATTACTTCTA TGGAGATTTG GTCAACTATT
            661 TGCATAAGAA TAGGGATAGC TTCCTGAGCC ACCACCCAGA GAAGCCAAAG AAAGAGCTGG
            721 ATATCTTTGG ACTGAACCCT GCTGATGAAA GCACACGGAG CTATGTTATT TTATCTTTTG
            781 AAAACAATGA TGACTACATG GNCATGAAGC ANGCTGATAC TACACAGTAT GTCCCCATGC
            841 TAGAAAGGAA AGAGGTTTCT AAATATTCCG ACATCCAGAG ATCACTCTAT GATCGTCCNG
            901 CCTCATATAA GAAGAAATCT ATGTTAGANT CANAAGTCAA AAACCTNCTT NCNNNGNNNA
            961 CTCNNANNNN TTACTTTATT GGNNTTGNTG ANCTNNNNCN NNCNNGTNGN CCNANNNNNN
          1021 NNNTTTTTGN NTTCNAAAAN NGNGNNNNNN NNNN
          //
          Primer: beta-gro_rev (Pr0512)
          Region: beta-globin pA, insert (FL) 3'
          Sequence file: RDB15465_B2Cgb.seq check
          >D04855B3_B2Cg_1_ beta-gro_rev_B09_06_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NTTGCCCCCT CNNTATAACA TGAATTTTAC NATAGCGAAA
             61 AAGAAAGAAC AATCAAGGGT CCCCAAACTC ACCCTGAAGT TCTCAGGATC CGATTATCTC
            121 GAGTTACAGG AAGCTGTCTT CCACCAGGTC TGAAGAGTCT ATGCCGATGT CGTCCATCAT
            181 GTCGATGTCT TCAATGGTCT CGTCCTCTCT CTTGATGAAG GTGGAACTGC TGGAACCCGT
            241 CTCAATGGCA CTCTCTTCAG AGGTCTGCGA GCTGTGTCTG TTCCTCTTGC CCAGGTCCTC
            301 CTCCTCAGGG ACAGGGTCAA TGTCAGGCAG AGGAATGATG TAGCCACTGT CAGCGCTCAG
            361 TCTCTGCTCA TCCAGACCAC CCTCCCAGTC CTTCAGCTTG TCTTCCTCGT TTTTGTAGGT
            421 GACACCAATG TATGCATTGT CTGAGTCCAC ACGCATGCGT GCCACAGCAG GATGGTCACT
            481 CTTCAGGAAG TCCAGGTGAA TTTTTTCATA ACTCTTTTTA TATTGTCCAG GCAGCAGATT
            541 CTCCACAATC TCACTCAGGT GGTAAAAGGA GGGTCTCTTC TCCGGCTCAC TGTTCCAGCA
            601 TTTCACCATG ATCTCGTAGA CTTCACTGGT AGCGTGGTCA GGCTTGGCCA TCCGGTACCC
            661 ACTCTTGATC TTATTGNAGA AAGTAGAATC CACCATCATG CCGGGGTAAN GGGTGNCACC
            721 NANGGAAAAG ATCTCCCANA NCANANNGNC NTAAGACCAG ACATCACTCA GTGTGGTGTA
            781 NAGGNTGNCA AANANGCTCT CNNANCNNTC CNCTTCNCGG GCANAAAGGN ANTGNCTCCC
            841 GANNCNTANT TCNAATCANG CNTGANGTCT CTGGNCNTGN CNAANTCNCN GANNNNCNCN
            901 NTTTTTNNTT GNGNNNNNAN NANNNNNCNA GCANCNNNNT NNNGGNGNNN NNNNNTTTTT
          961 TNANNNNAAA CNNCNNTNNN NNNGNNNNNN NNNN
          //
          Primer: PDGFRA_F3 (Pr1185)
          Region: insert (FL) mid
          Sequence file: RDB15465_B2Cgc.seq check
          >D04855B3_B2Cg_1_PDGFRA_F3_F08_17_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN GNNNNNNTTA NNTNNTCNCN GAGTATTACT TCTATGGAGA
             61 TTTGGTCAAC TATTTGNNTA AGAATAGGGA TAGCTTCCTG AGCCACCACC CAGAGAAGCC
            121 AAAGAAAGAG CTGGATATCT TTGGACTGAA CCCTGCTGAT GAAAGCACAC GGAGCTATGT
            181 TATTTTATCT TTTGAAAACA ATGATGACTA CATGGACATG AAGCAGGCTG ATACTACACA
            241 GTATGTCCCC ATGCTAGAAA GGAAAGAGGT TTCTAAATAT TCCGACATCC AGAGATCACT
            301 CTATGATCGT CCAGCCTCAT ATAAGAAGAA ATCTATGTTA GACTCAGAAG TCAAAAACCT
            361 CCTTTCAGGT GATAACTCAG AAGGCCTTAC TTTATTGGAT TTGTTGAGCT TCACCTATCA
            421 AGTTGCCCGA GGAATGGAGT TTTTGGCTTC AAAAAATTGT GTCCACCGTG ATCTGGCTGC
            481 TCGCAACGTC CTCCTGGCAC AAGGAAAAAT TGTGAAGATC TGTGACTTTG GCATGGCCAG
            541 AGACATCATG CATGATTCGA ACTATGTGTC GGGAGGCAGT ACCTTTCTGC CCGTGAAGTG
            601 NATGGCTCCT GAGAGCATCT TTGACAACCT CTACACCACA CTGAGTGATG TCTGGTCTTA
            661 TGGCATTCTG CTCTGGGAGA NCTTTTCCCT TGNTGGCACC NCTTACCCCG GCATGATGGT
            721 GGATTCTACT TTCTACAATA AGATCAAGAG TGGGTACCGG ATGGCCAAGC CTGACCACGC
            781 TACCNGTGAA GTCTACNANA TCATGGTGAA ATGNTGGAAC AGTGANNCGG ANAANANANC
            841 CTCCTTTTAC CNCNNGANNG ANANTGNGNA GAATCTGNTG NNNGNNNNNT ATAAAAANAG
            901 TNATGAAAAA ATTCNCNNNN NNTNNNNGNA NANNGANNNT CCNGNTGNGN NNNNNNTGNN
          961 NGNNNNNTCN NANNNGNATA CNTNNNNNNN NNN
          //
          Primer: SV40pro_ori_F (Pr0731)
          Region: SV40 pro_ori, beta-globin pA
          Sequence file: RDB15465_B2Cgd.seq check
          >D04855B3_B2Cg_1_ SV40pro_ori_F_C09_09_ABI24.ab1
              1 NNNNNNNNNN NNNNCNNNNG CNNCCTCGNC CTCTGNNCTA TTCCNGAAGT AGTGAAGAGG
             61 CTTTTTTGGA GGCCTAGGCT TTTGCAAAAA GCTCCTCGAG GGATCTCCAT AAGAGAAGAG
            121 GGACAGCTAT GACTGGGAGT AGTCAGGAGA GGAGGAAAAA TCTGGCTAGT AAAACATGTA
            181 AGGAAAATTT TAGGGATGTT AAAGAAAAAA ATAACACAAA ACAAAATATA AAAAAAATCT
            241 AACCTCAAGT CAAGGCTTTT CTATGGAATA AGGAATGGAC AGCAGGGGGC TGTTTCATAT
            301 ACTGATGACC TCTTTATAGC CAACCTTTGT TCATGGCAGC CAGCATATGG GCATATGTTG
            361 CCAAACTCTA AACCAAATAC TCATTCTGAT GTTTTAAATG ATTTGCCCTC CCATATGTCC
            421 TTCCGAGTGA GAGACACAAA AAATTCCAAC ACACTATTGC AATGAAAATA AATTTCCTTT
            481 ATTAGCCAGA AGTCAGATGC TCAAGGGGCT TCATGATGTC CCCATAATTT TTGGCAGAGG
            541 GAAAAAGATC TCAGTGGTAT TTGTGAGCCA GGGCATTGGC CACACCAGCC ACCACCTTCT
            601 GATAGGCAGC CTGCACCTGA NGAGTGAATT CTTTGCCAAA ATGATGAGAC AGCACAACAA
            661 CCAGCACGTT GCCCAGGANC TGTANGAAAG AGAAGAANGN ATGAACATGG TTAGCAGANG
            721 GGCCCGGTTT GNANTCNGAN TATTTTATCC TCATCTCNAA CAGTGTATAT CATTGTAACN
            781 NTAAAGAGAA AGGNAGGANG ANGANNAGGG NGTAGTTGNT TCTANNNATN ANAANANTTC
            841 CNCGCCNGCC NNAANTTATA TGCNNAAATA TTCTACCTTA TCATTTNNTT ATNNNNNTTG
            901 NNCTCTAAAA CTGNGCTGNA NNANNNNATN NNATACCNNG ANNGNCNTNN NAAAAAAANN
          961 GANNNNNNNA NGNANNNANN NNNNNNNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Ibata, M., Leukemogenic kinase FIP1L1-PDGFRA and a small ubiquitin-like modifier E3 ligase, PIAS1, form a positive cross-talk through their enzymatic activities. Cancer Sci. 108 (2): 200-207 (2017). PMID 27960034.
          reference Iwasaki, J., FIP1L1 presence in FIP1L1-RARA or FIP1L1-PDGFRA differentially contributes to the pathogenesis of distinct types of leukemia. Ann. Hematol. 93 (9): 1473-1481 (2014). PMID 24763514.

          2023.05.01

          GNP_filter3_RDBDEP_html_230501.pl