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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

UnaG/pcDNA3-FLAG

Expression vector of eel UnaG, bilirubin-inducible fluorescent protein.

Catalog number RDB15703
Resource name UnaG/pcDNA3-FLAG
Clone info. Expression vector of eel UnaG, bilirubin-inducible fluorescent protein.
Vector backbone pcDNA3-FLAG (Plasmid)
Selectable markers Amp^r
Gene/insert name Anguilla japonica (Japanese eel) UnaG cDNA
Reference of insert sequence AB763906 (DDBJ accession number)
Depositor|Developer Miyawaki, Atsushi |
Other clones in our bank

External Database
Anguilla japonica (Japanese eel) UnaG

            Reference sequence
              
             

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
            2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
            3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
            Kumagai, A., Ando. R,, Miyatake, H., Greimel, P., Kobayashi, T., Hirabayashi, Y., Shimogori, T., Miyawaki, A. (2013) A Bilirubin-Inducible Fluorescent Protein from Eel Muscle. Cell, 153 (7): 1602-1611.
            4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
            5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
            Ordering Please visit Information of Request for Distribution.[link] 
            Order form 
            [Credit Card Payment]  [Bank Transfer Payment]
            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            Approval form [Word] by Dr. Atsushi Miyawaki
            Remarks ((Additional form)) Approval Form(FormD)
            Please obtain written approval of Dr. Atsushi Miyawaki.
            提供案内 (日本国内) [open/close]

            提供条件 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
            2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
            3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
            Kumagai, A., Ando. R,, Miyatake, H., Greimel, P., Kobayashi, T., Hirabayashi, Y., Shimogori, T., Miyawaki, A. (2013) A Bilirubin-Inducible Fluorescent Protein from Eel Muscle. Cell, 153 (7): 1602-1611.
            4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
            5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            宮脇敦史博士による提供承諾書 [Word]
            備考 【追加提供依頼書】承諾書(FormD)
            提供条件は英文のまま記載してください。
            宮脇敦史博士の書面による提供承諾が必要です。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB15703 UnaG/pcDNA3-FLAG DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            How to cite this biological resource

            Materials & Methods section:

            The UnaG/pcDNA3-FLAG was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15703).

            Reference section:

            Kumagai, A., Ando, R., Miyatake, H., Greimel, P., Kobayashi, T., Hirabayashi, Y., Shimogori, T., Miyawaki, A., A bilirubin-inducible fluorescent protein from eel muscle. Cell 153 (7): 1602-1611(2013). PMID 23768684.
            Miyawaki, A., Kumagai, A., Novel polypeptide exhibiting fluorescent properties and use thereof. Patent publication US 2016 0009771 A1.

            Further references such as user reports and related articles (go to bottom)


            References and tips

            Featured content

            Featured content Fluorescent protein resources (English text)
            Featured content Fluorescent protein resources (Japanese text)
            Featured content Sensor and Visualization (English text)

            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB15703_A7Lcp1-3.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: CMV_Forward (Pr0016)
            Region: CMV pro,insert 5'(FLAG,UnaG 5')
            Sequence file: RDB15703_A7Lcc.seq check
            >D05018C7_A7Lc_2_CMV-Forward_D02_11_ABI24.ab1
                1 NNNNNNNNNN NCCNTNNNNG CNNTGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG
               61 CAGAGCTCTC TGGCTAACTA GAGAACCCAC TGCTTACTGG CTTATCGAAA TTAATACGAC
              121 TCACTATAGG GAGACCCAAG CTTGCCACCA TGGATTACAA GGATGACGAC GATAAGGGAT
              181 CCGGTGGTTC TGGTATGGTC GAGAAATTTG TTGGCACCTG GAAGATCGCA GACAGCCATA
              241 ATTTTGGTGA ATACCTGAAA GCTATCGGAG CCCCAAAGGA ATTAAGCGAT GGTGGGGATG
              301 CCACGACGCC GACATTGTAC ATCTCCCAGA AGGACGGAGA CAAAATGACA GTGAAAATAG
              361 AGAATGGACC TCCTACGTTC CTTGACACTC AAGTAAAGTT CAAATTAGGG GAGGAGTTCG
              421 ACGAATTTCC TTCTGATCGA AGAAAAGGCG TAAAATCTGT CGTGAACTTG GTGGGAGAGA
              481 AGCTGGTGTA CGTACAAAAG TGGGACGGCA AGGAGACGAC GTATGTCCGA GAGATAAAGG
              541 ACGGTAAACT GGTCGTGACA CTTACGATGG GAGACGTCGT GGCTGTGCGC AGCTACCGGA
              601 NGGCGACGGA ATGAGAATTC TGCAGATAGC TAGCTAGCTA TCCATCACAC TGGCGGCCGC
              661 TCGAGCATGC ATCTAGAGGG CCCTATTCTA TAGTGTCACC TAAATGCTAG AGCTCGCTGA
              721 TCAGCCTCGA CTGTGCCTTC TAGTTGCCAG CCATCTGTTG TTTGCCCCTC NCCCGTGCCT
              781 TCCTTGACCC TGGAANGTGC CACTCNNACT GTCCTTTCCT AATAAAATGA NGAAATTGCA
              841 TCGCATNNNC TGANTANGNG TCATTCTATT CTGGGGGNNN GGGGNGGGGN NANGNNANCN
              901 ANGGGGGANN NNTGGGGNAN NANNNNNNCN NNNNTGCNGG GGNNNGCNGN NNGGNNNNNN
            961 NNNN
            //
            Primer: BGH_rev2 (Pr0606)
            Region: bGH pA,insert(UnaG,FLAG)
            Sequence file: RDB15703_A7Lcd.seq check
            >15703_15703_B3BeF_BGH_rev2_H01_22_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNNN GGNNGNNGGG CNNACNACAG ATGGCTGGCA ACTAGAAGGC
               61 ACAGTCGAGG CTGATCAGCG AGCTCTAGCA TTTAGGTGAC ACTATAGAAT AGGGCCCTCT
              121 AGATGCATGC TCGAGCGGCC GCCAGTGTGA TGGATAGCTA GCTAGCTATC TGCAGAATTC
              181 TCATTCCGTC GCCCTCCGGT AGCTGCGCAC AGCCACGACG TCTCCCATCG TAAGTGTCAC
              241 GACCAGTTTA CCGTCCTTTA TCTCTCGGAC ATACGTCGTC TCCTTGCCGT CCCACTTTTG
              301 TACGTACACC AGCTTCTCTC CCACCAAGTT CACGACAGAT TTTACGCCTT TTCTTCGATC
              361 AGAAGGAAAT TCGTCGAACT CCTCCCCTAA TTTGAACTTT ACTTGAGTGT CAAGGAACGT
              421 AGGAGGTCCA TTCTCTATTT TCACTGTCAT TTTGTCTCCG TCCTTCTGGG AGATGTACAA
              481 TGTCGGCGTC GTGGCATCCC CACCATCGCT TAATTCCTTT GGGGCTCCGA TAGCTTTCAG
              541 GTATTCACCA AAATTATGGC TGTCTGCGAT CTTCCAGGTG CCAACAAATT TCTCGACCAT
              601 ACCAGAACCA CCGGATCCCT TATCGTCGTC ATCCTTGTAA TCCATGGTGG CAAGCTTGGG
              661 TCTCCCTATA GTGAGTCGTA TTAATTTCGA TAAGCCNGTA AGCAGTGNGT TCTCTAGTTA
              721 GCCAGANAGC TCTGCTTATA TAGANCTCCC ACCGNACACG CCTACCGNCC ATTTGCGTCA
              781 ATGGGGCGNA NTTGTTACNA CATTTTGNAA AGTCCCNTTG ANTTTGGNGC CNAAACAAAC
              841 TCCNATTGAN GTNNNTGGGN NNNNNNNTNN NNNNCCCCNN NNNTCNNNNN GNTNNCNNNN
            901 CCNNTNGANG TNCTGNNNAA NNNNNNNNNN
            //
            Primer: SV40pro_F (Pr0110)
            Region: NeoR
            Sequence file: RDB15703_A7Lce.seq check
            >D05018C7_A7Lc_2_SV40pro_F_E02_14_ABI24.ab1
                1 NNNNNNNNNN NNNNNNCNNN NNNNTGNNGN NGCTTTTTTG GANGCCTAGG CTTTTGCAAA
               61 AAGCTCCCGG GANCTTGTAT ATCCNTTTTC GGATCTGATC AAGAGACAGG ATGAGGATCG
              121 TTTCGCATGA TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG
              181 CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG
              241 CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT
              301 GAACTGCAGG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA
              361 GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG
              421 GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT
              481 GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA
              541 CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG
              601 GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGCGCATG
              661 CCCGACGGCG AGGNTCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG
              721 GANNATGGCC GCTTTTCTGG ATTCATCGAC TGTGNCCGGC TGGGTGTGGC GGANCGCTAT
              781 CANGACATAG CGTTGGCTAC CCGTGATATT GCTGAANNNC TTGGCGGCGA ATGGGCTGAN
              841 CGCTTCCTCG NGCTTTACNG TATCGCCGCN CCCNANTCNC ANCGCATCGC CTTCNATCGC
            901 CNNNNNGNNN ANNNNNNCNG ANCGGNNNTN NNGGGNNNCN NAATNNNNNN NNNN
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Kumagai, A., A bilirubin-inducible fluorescent protein from eel muscle. Cell 153 (7): 1602-1611(2013). PMID 23768684.
            original Miyawaki, A., Novel polypeptide exhibiting fluorescent properties and use thereof. Patent publication US 2016 0009771 A1.
            reference Mu, A., Enhancements of the production of bilirubin and the expression of β-globin by carbon monoxide during erythroid differentiation. FEBS Lett. 590 (10): 1447-1454 (2016). PMID 27087140.
            reference Iwatani, S., Fluorescent protein-based detection of unconjugated bilirubin in newborn serum. Sci. Rep. 6: 28489 (2016). PMID 27324682.
            review Miyawaki, A., Exploiting the cyanobacterial light-harvesting machinery for developing fluorescent probes. Nat. Methods 13 (9): 729-730 (2016). PMID 27575623.

            2023.05.01

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