Resource data sheet
Prev.
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pSPB1

Expression vector of Streptococcus pneumoniae G54 peptidase domain (PEP) of BlpA.

Catalog number RDB15715
Resource name pSPB1
Clone info. Expression vector of Streptococcus pneumoniae G54 peptidase domain (PEP) of BlpA, tagged with His at C-termonus.
Comment Correspinding nucleotide 475628 to 476077 of GenBank CP001015 was chemically synthesized.
Vector backbone pET21-b (plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r
Gene/insert name Streptococcus pneumoniae G54 blpA genomic
Depositor|Developer Ishii, Seiji |
Other clones in our bank
External Database
Streptococcus pneumoniae G54 blpA
          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Ishii, S et al. Sci. Rep. 7(1): 4029, 2017)
          Ordering Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
          Order form           [Credit Card Payment]  [Bank Transfer Payment]
          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Ishii, S et al. Sci. Rep. 7(1): 4029, 2017)。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB15715 pSPB1 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Ishii, S., High-throughput Screening of Small Molecule Inhibitors of the Streptococcus Quorum-sensing Signal Pathway. Sci. Rep. 7 (1): 4029 (2017). PMID 28642545.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB15716_A7Lcp1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pBR_BamHI_R2
          Sequence file: RDB15716_A7Lca.seq check
          1
          Primer: lacpro_F
          Sequence file: RDB15716_A7Lcb.seq check
          1

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          original Ishii, S., High-throughput Screening of Small Molecule Inhibitors of the Streptococcus Quorum-sensing Signal Pathway. Sci. Rep. 7 (1): 4029 (2017). PMID 28642545.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl