Please review the QC test results indicated by icon below as well as clone information before placing your order.

pcDNA3 Venus-Akaluc

Expression vector of Akaluc luciferase.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Iwano, S. et al., Single-cell bioluminescence imaging of deep tissue in freely moving animals. Science 359 (6378): 935-939 (2018). 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
Ordering	Order form [Credit Card Payment]  [Bank Transfer Payment] MTA, for use for not-for-profit academic purpose [Word] Approval form [Word] by Dr. Atsushi Miyawaki Please visit Information of Request for Distribution.[link]
Remarks	Please obtain written approval of Dr. Atsushi Miyawaki.

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提供条件	1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Iwano, S. et al., Single-cell bioluminescence imaging of deep tissue in freely moving animals. Science 359 (6378): 935-939 (2018). 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
提供依頼	提供依頼書 [Word] 提供同意書 (MTA, 非営利学術目的用)[Word] 宮脇敦史博士による提供承諾書 [Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
備考	提供条件は英文のまま記載してください。 宮脇敦史博士の書面による提供承諾が必要です。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

Reference section:

Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB15781_A8B8p1-4.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016) Region: CMV pro,T7 pro,insert 5' (mVenus 5') Sequence file: RDB15781_A8Cdg.seq
>D04498K3_A8B8_2_CMV-Forward_A05_02_ABI24.ab1     1 NNNNNNNNNN NNCNTTGNNG CNNNGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG    61 CAGAGCTCTC TGGCTAACTA GAGAACCCAC TGCTTACTGG CTTATCGAAA TTAATACGAC   121 TCACTATAGG GAGACCCAAG CTTGCCACCA TGGTGAGCAA GGGCGAGGAG CTGTTCACCG   181 GGGTGGTGCC CATCCTGGTC GAGCTGGACG GCGACGTAAA CGGCCACAAG TTCAGCGTGT   241 CCGGCGAGGG CGAGGGCGAT GCCACCTACG GCAAGCTGAC CCTGAAGCTG ATCTGCACCA   301 CCGGCAAGCT GCCCGTGCCC TGGCCCACCC TCGTGACCAC CCTGGGCTAC GGCCTGCAGT   361 GCTTCGCCCG CTACCCCGAC CACATGAAGC AGCACGACTT CTTCAAGTCC GCCATGCCCG   421 AAGGCTACGT CCAGGAGCGC ACCATCTTCT TCAAGGACGA CGGCAACTAC AAGACCCGCG   481 CCGAGGTGAA GTTCGAGGGC GACACCCTGG TGAACCGCAT CGAGCTGAAG GGCATCGACT   541 TCAAGGAGGA CGGCAACATC CTGGGGCACA AGCTGGAGTA CAACTACAAC AGCCACAACG   601 TCTATATCAC CGCCGACAAG CAGAAGAACG GCATCAAGGC CAACTTCAAG ATCCGCCACA   661 ACATCGAGGA NGGCGGCGTG CAGCTCGCCG ANCACTACCA GCAGAACACC CCCATCGGCG   721 ANGGCCCCGN GCTGCTGNCC GACNNCCACT ACCTGANCTA CCAGTCCGCC CTGANCAAAG   781 NNCCNNACGA NAAGCGCGAT CACNTGGNNC TGCTNGANTN CGNGANCGCC GCNNGGNTCA   841 NTNTCGGCNT NGNNNANCTG TANNNNNNAT NCNNNNNANN NNGNCNAAAA NNTTAANNAN 901 GGNCCNNNNN NNNNN //
Primer: BGH_rev2 (Pr0606) Region: bGH pA,insert 3' (Akaluc 3') Sequence file: RDB15781_A8Cdh.seq
>D04498K3_A8B8_2_BGH_rev2_B05_05_ABI24.ab1     1 NNNNNNNNNN NNNNNNNGGG GGNNGGGNAN CNACAGATGG CTGGCAACTA GAAGGCACAG    61 TCGAGGCTGA TCAGCGAGCT CTAGCATTTA GGTGACACTA TAGAATAGGG CCCTCTAGAT   121 GCATGCTCGA GCGGCCGCCA GTGTGATGGA TATCTGCAGA ATTCTTACAC GGCGATCTTG   181 CCGTCCTTCT TGGCCTTAGT GAGAATCTCG CGGATCTTGC GGGCGTCTAA CTTGCCGGTC   241 GATCCTCTAG GGACTTCATC CACAAACACA ACACCACCGC GCAGCTTCTT GGCGGTTGTA   301 ACCTGGCTGG CCACATAGTC CACGATCTCT TTCTCGGTCA TGGTTTTACC GTGTTCCAAC   361 ACGACGACTG CGGCGGGCAG CTCGCCGGCA TCGTCGTCGG GCAGGCCGGC GACTCCGGCG   421 TCGAAGATGT AGGGGTGTTG CAGCAGGATG CCCTCCAGTT CGGCTGGGGC TACCTGGTAG   481 CCCTTGTATT TGATCAGGCT CTTCAGCCGG TCCACGATGA AGAAGTGCTC GTCCTCGTCC   541 CAGTAGGCGA TGTCGCCGCT GTGCAGCCAG CCGTCCTTGT CGATGAGAGC GTTCGTAGCC   601 TCGGGGTTGT TAACGTAGCC GCTCATGATC ATGGGGCCAC GGACGCACAG CTCACCGCGC   661 TGGTTCACAC NNNGTGTCTT ANCGGTGANC AAGTCTACCA CCTTAGCNTC GAANAANGGC   721 ACCACNTTGC CTACTGANCN NGGCTTANGG TCCNNNTNNG GGGTGATNNT GNNGGNGTTN 781 GTTGTTTNTG TCNNNNNTAN NNNNNNNNN //
Primer: mVenus-M_F (Pr0574) Region: insert mid (mVenus 3', Akaluc 5') Sequence file: RDB15781_A8Cdi.seq
>15781_15781_B2EiF_mVenus-M_F_F06_18_ABI24.ab1     1 NNNNNNNNNN NNNNANGGCN TCNAGGCNNA CTTNNAGANC CGCCACAACA TCGAGGACGG    61 CGGCGTGCAG CTCGCCGACC ACTACCAGCA GAACACCCCC ATCGGCGACG GCCCCGTGCT   121 GCTGCCCGAC AACCACTACC TGAGCTACCA GTCCGCCCTG AGCAAAGACC CCAACGAGAA   181 GCGCGATCAC ATGGTCCTGC TGGAGTTCGT GACCGCCGCC GGGATCACTC TCGGCATGGA   241 CGAGCTGTAC AAGGGATCCA TGGAAGATGC CAAAAACATT AAGAAGGGCC CAGCGCCGTT   301 CTACCCACTC GAAGACGGGA CCGCCGGCGA GCAGCTGCAC AAAGCCATGA AGCGCTACGC   361 CCTGGTGCCC GGCGCCATCG CCTTTACCGA CGCACATATT CAGGTGGACG TTACCTACGC   421 CGAGTACTTC GAGATGAGCG TTCGGCTGGC AGAAGCTATG AGGCGCTATG GGCTGAATAC   481 AAACCATCGG ATCGTGGTGT GCAGCGAGAA TAGCTCGCAG TTCTTCATGC CCGTGTTGGG   541 TGCCCTGTTC ATCGGTGTGG CTGTGGCCCC AGCTAACGAC ATCTACAACG AGCGCGAGCT   601 GCTGAACAGC ATGGGCATCA GCCAGCCCAC CGTCGTATTC GTGAGCAAGA AAGGGCTGCG   661 AAAGGTCCTC AACGTGCAAA AGAAGCTACC GATCATACGA AAGATCATCA TCATGGATAG   721 CAAGACCGAC TACCAGGGCT TCCAAAGCAT GTACACCTTC GTGACTTCCC ATTTGCCACC   781 CAGCTTCAAC GAGTACNANT TCGTGCCCGA NAGCTTCNAC CGGNNNAAAA CCATCGCCCT   841 GATCATGAAC AGTAGTGGTA GTACAGGATT ACCCAAGGGC GTANCCCTAC CGCACCGCAC   901 CGCNNGTGTC CNANTCNGNC NTGNCCGCGA NCCCNTCTTC NNNTNNCAGA ANNTCCCCNA   961 NNCCGNNATC CNCAGCGNGN NNNCNTTTNN CCACGGNTTC NNNNTGNNCN NCNNGCTGGG 1021 NNANNNGNTN NGNNGNNTNN NGNNNNNNNN NN //
Primer: Venus-C_F2 (Pr0454) Region: insert mid (Akaluc mid) Sequence file: RDB15781_A8Cdj.seq
>D04498K3_A8B8_2_Venus-C_F2_C05_08_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNTCTCGNN NTGGNNGAGC TGTACNAGGG NNCCATGGAA    61 GANGCCAAAA ACATTAAGAA GGGCCCAGCG CCGTTCTACC CACTCGAAGA CGGGACCGCC   121 GGCGAGCAGC TGCACAAAGC CATGAAGCGC TACGCCCTGG TGCCCGGCGC CATCGCCTTT   181 ACCGACGCAC ATATTCAGGT GGACGTTACC TACGCCGAGT ACTTCGAGAT GAGCGTTCGG   241 CTGGCAGAAG CTATGAGGCG CTATGGGCTG AATACAAACC ATCGGATCGT GGTGTGCAGC   301 GAGAATAGCT CGCAGTTCTT CATGCCCGTG TTGGGTGCCC TGTTCATCGG TGTGGCTGTG   361 GCCCCAGCTA ACGACATCTA CAACGAGCGC GAGCTGCTGA ACAGCATGGG CATCAGCCAG   421 CCCACCGTCG TATTCGTGAG CAAGAAAGGG CTGCGAAAGG TCCTCAACGT GCAAAAGAAG   481 CTACCGATCA TACGAAAGAT CATCATCATG GATAGCAAGA CCGACTACCA GGGCTTCCAA   541 AGCATGTACA CCTTCGTGAC TTCCCATTTG CCACCCAGCT TCAACGAGTA CGACTTCGTG   601 CCCGAGAGCT TCGACCGGGA CAAAACCATC GCCCTGATCA TGAACAGTAG TGGTAGTACA   661 GGATTACCCA AGGGCGTAGC CCTACCGCAC CGCACCGCTT GTGTCCGATT CAGTCATGCC   721 CGCGACNNCA TCTTCGGCTA CCAGAACATC CCCGACACCG CTATCCTCAG CGTGGTGCCA   781 TTTCACCACG GCTTCNGCAT GTTCACCACG CTGGGCTANT TGNTCTGCGG CTTTCGGGTC   841 GTGCTCATGT ACNGCTTCGA NGANGANCTA TTCTTGCGCA GCTNGCAAGA NTATAAGANT   901 CAATCTGNCN NGCTGGNGNC CACACNATTT AGCTGCCNNG CTNAGANCNN TNNNNTCGNN 961 NNNNNCNANC NNNNNAGCNN GNNNNANATC GNCNNNNNGN NNGNNNNNNN NNN //
Primer: Akaluc 576-593 (Pr0539) Region: insert mid (Akaluc mid) Sequence file: RDB15781_A8Cdk.seq
>D04498K3_A8B8_2_Akaluc 576-593_D05_11_ABI24.ab1     1 NNNNNNNNNN GNGNNNCCNT NNCGCNCCGC ACCGCTTGTG TCCGATTCNG TCATGCCCGC    61 GACCCCATCT TCGGCTACCA GAACATCCCC GACACCGCTA TCCTCAGCGT GGTGCCATTT   121 CACCACGGCT TCGGCATGTT CACCACGCTG GGCTACTTGA TCTGCGGCTT TCGGGTCGTG   181 CTCATGTACC GCTTCGAGGA GGAGCTATTC TTGCGCAGCT TGCAAGACTA TAAGATTCAA   241 TCTGCCCTGC TGGTGCCCAC ACTATTTAGC TGCCTCGCTA AGAGCACTCT CATCGACAAG   301 TACGACCTAA GCAGCTTGCG CGAGATCGCC AGCGGCGGGG CGCCGCTCAG CAAGGAGGTA   361 GGTGAGGCCG TGGCCAAACG CTTCCGCCTA CCAGGCATCC GCCAGGGCTA TGGCCTGACA   421 GAAACAACCA ACGCCGTCAT GATCACCCCC GAGGGGGACC GTAAGCCTGG CTCAGTAGGC   481 AAGGTGGTGC CCTTCTTCGA GGCTAAGGTG GTAGACTTGG TCACCGGTAA GACACTGGGT   541 GTGAACCAGC GCGGTGAGCT GTGCGTCCGT GGCCCCATGA TCATGAGCGG CTACGTTAAC   601 AACCCCGAGG CTACGAACGC TCTCATCGAC AAGGACGGCT GGCTGCACAG CGGCGACATC   661 GCCTACTGGG ACGANGACGA NCACTTCTTC ATCGTGGANC GGCTGAAGAG CCTGATCAAA   721 TACAAGGGCT ACCAGGTAGC CCCAGCCGAA CTGGANGGCA TCCTGCTGCA ACACCNCTAC   781 NTCTTCGACG CCNGANNCGC CGGCCTGNCC GANGACGATG NCGGCGANCT GCCCGCCGCA   841 NNCGNCGTGT TNGAACACGG TAAAACCATG ANCGANAAAG ANATCGNGGN NNATGNGGCC   901 AGCCAGGNTN NNACCGNCNN NNNNNCTGNN NNNNNGNNNN NNNNTNNNNG NNGNNNNNCN 961 NANNNGNNNC GNNNNNNNNN N //
Primer: SV40pro_F (Pr0110) Region: SV40pro_ori, NeoR Sequence file: RDB15781_A8Cdl.seq
>D04498K3_A8B8_2_SV40pro_F_F05_17_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NANTNGTGNN GNNNNTTTTT TGGANGCCTA GGCTTTTGCA    61 AAAAGCTCCC GGGAGCTTGT ATATCCATTT TCGGATCTGA TCAAGAGACA GGATGAGGAT   121 CGTTTCGCAT GATTGAACAA GATGGATTGC ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA   181 GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG CTCTGATGCC GCCGTGTTCC   241 GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC CGACCTGTCC GGTGCCCTGA   301 ATGAACTGCA GGACGAGGCA GCGCGGCTAT CGTGGCTGGC CACGACGGGC GTTCCTTGCG   361 CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG GCTGCTATTG GGCGAAGTGC   421 CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA GAAAGTATCC ATCATGGCTG   481 ATGCAATGCG GCGGCTGCAT ACGCTTGATC CGGCTACCTG CCCATTCGAC CACCAAGCGA   541 AACATCGCAT CGAGCGAGCA CGTACTCGGA TGGAAGCCGG TCTTGTCGAT CAGGATGATC   601 TGGACGAAGA GCATCAGGGG CTCGCGCCAG CCGAACTGTT CGCCAGGCTC AAGGCGCGCA   661 TGCCCGACGG CGAGGATCTC GTCGTGANCC ATGGCGATGC CTGCTTGCCG AATATCATGG   721 TGGAAAATGN NCGCTTTTCT GGATTCATCG ACTGTGGNCG GCTGGGTGTG GCGGANCGCT   781 ATCANGANAT ANCGTTGGCT ACCCGTGATA TTGCTGAANA NCTTGGNNGG CNAATGGGCT   841 GANNGCTTCC TCGNGCTTTA CNGNATCGCN GCNNCCNATN CGCAGCGCAT CGCNNNCNAT   901 NNCNNTCNNG ANNANTTNTT CTGANCNGNN NTCTNGGGNN NCNAAATGAN NNANCNNNNG 961 ACGCCNNNNC NNNNCNNNNN NNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2024.04.04

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