Resource data sheet
DNA Bank Top
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pcDEF3_Myc-mMyoD (#RDB15787)

Expression vector of mouse myogenic differentiation 1 (MyoD).

Clone info. Expression vector of mouse myogenic differentiation 1 (MyoD), tagged with Myc at N-terminus.
Comment Expression was confirmed by the depositor with immunoprecipitation and western blot. Low copy number plasmid. Refer Goldman LA. et al., Biotechniques 21 (6): 1013-1015 (1996), PMID 8969825 for the pDEF3 vector.
Vector backbone pcDEF3 (plasmid)
Size of vector backbone 6.1 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells).
Gene/insert name mouse Myod1 cDNA
Depositor|Developer Kokabu, Shoichiro |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kokabu, S. et al. J. Biol. Chem. 292(31): 12885-12894, 2017)
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Kokabu, S. et al. J. Biol. Chem. 292(31): 12885-12894, 2017)。

Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB15787 pcDEF3_Myc-mMyoD Under QC test. Please contact us. DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

How to cite this biological resource

Materials & Methods section:

The pcDEF3_Myc-mMyoD was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15787).

Reference section:

Kokabu, S., Nakatomi, C., Matsubara, T., Ono, Y., Addison, W.N., Lowery, J.W., Urata, M., Hudnall, A.M., Hitomi, S., Nakatomi, M., Sato, T., Osawa, K., Yoda, T., Rosen, V., Jimi, E., The transcriptional co-repressor TLE3 regulates myogenic differentiation by repressing the activity of the MyoD transcription factor. J. Biol. Chem. 292 (31): 12885-12894 (2017). PMID 28607151. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

References

Original, user report and related articles

original Kokabu, S., The transcriptional co-repressor TLE3 regulates myogenic differentiation by repressing the activity of the MyoD transcription factor. J. Biol. Chem. 292 (31): 12885-12894 (2017). PMID 28607151. [link to RRC of NBRP]