RDB15789 - pMGB

pMGB

Expression vector of EFGP and ble under the control of Perkinsus marinus MOE gene promoter.

Catalog number	RDB15789
Resource name	pMGB
Clone info.	Bicistronic expression vector of EGFP and Sh-ble intervened by a self-cleaving 2A peptide, which is controled under the Perkinsus marinus MOE (PmMOE) promoter. This clone is for a drug selection system using bleomycin for transfecting Perkinsus species. Perkinsus is sensitive to bleomycin, and 100 microgram/ml of this drug completely blocks its proliferation.
Comment	Expression was confirmed by the depositor with fluorescence microscopy.
Vector backbone	pCR4-TOPO (Plasmid)
Size of vector backbone	4 kb
Selectable markers	Amp^r, Kan^r.
Gene/insert name	Perkinsus marinus MOE genomic DNA Aequorea victoria GFP cDNA Streptoalloteichus hindustanus bleomycin resistance gene genomic DNA
Depositor\|Developer	Nozaki, Tomoyoshi \| Matsuzaki, Motomichi \|

Other clones in our bank

External Database
Perkinsus marinus MOE

Aequorea victoria GFP

Streptoalloteichus hindustanus bleomycin resistance gene

Reference sequence

Sequence	RDB15789z.seq

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of one of the literature designated by the DEPOSITOR is requested (Sakamoto, H. et al., Parasitol. Int. 69: 13-16 (2018); Sakamoto, H. et al., J. Eukaryot. Microbiol. in press).

Ordering Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.
Order form [Credit Card Payment] [Bank Transfer Payment]
Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

提供案内 (日本国内) [open/close]

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of one of the literature designated by the DEPOSITOR is requested (Sakamoto, H. et al., Parasitol. Int. 69: 13-16 (2018); Sakamoto, H. et al., J. Eukaryot. Microbiol. in press).
Ordering	Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. Order form [Credit Card Payment] [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定するいずれかの文献を引用する (Sakamoto, H. et al., Parasitol. Int. 69: 13-16 (2018); Sakamoto, H. et al., J. Eukaryot. Microbiol. in press)。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Catalog #	Resource name	Availability	Shipping form	Fee (non-profit org.)
RDB15789	pMGB	Under QC test. Please contact us.	DNA solution

Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

Original reference

original	Sakamoto, H., A Novel 2A-peptide-containing Plasmid to Generate Stable Perkinsus marinus Cells Expressing Organelle-targeted Genes. J. Eukaryot. Microbiol. 68 (5): e12861 (2021). PMID 34051022.
original	Sakamoto, H., Puromycin selection for stable transfectants of the oyster-infecting parasite Perkinsus marinus. Parasitol. Int. 69: 13-16 (2018). PMID 30389616.

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

Original, user report and related articles

original	Sakamoto, H., A Novel 2A-peptide-containing Plasmid to Generate Stable Perkinsus marinus Cells Expressing Organelle-targeted Genes. J. Eukaryot. Microbiol. 68 (5): e12861 (2021). PMID 34051022.
original	Sakamoto, H., Puromycin selection for stable transfectants of the oyster-infecting parasite Perkinsus marinus. Parasitol. Int. 69: 13-16 (2018). PMID 30389616.

2023.04.06

GNP_filter3_RDBDEP_html_230405.pl