Resource data sheet
Prev.
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

p3xFLAG-Myc-CMV-AGO2-PAZ-mut

Expression vector of human AGO2 mutated PAZ domain (Y311Q/F312L).

Catalog number RDB15798
Resource name p3xFLAG-Myc-CMV-AGO2-PAZ-mut
Clone info. Expression vector of human Argonaute protein 2 (AGO2) mutated PAZ domain (Y311Q/F312L), tagged with FLAG at N-terminus, CMV promoter. Please note Myc-tag is not available. See Fig. 4b of Deguchi, S. et al., Oncogene, 36 (32): 4629-4640, 2017 for outline of construction.
Comment Expression was confirmed by the depositor with western blotting.
Vector backbone p3xFLAG-Myc-CMV (plasmid)
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells).
Gene/insert name human AGO2 cDNA
Depositor|Developer Kondo, Yutaka |
Other clones in our bank

External Database
human AGO2

          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Katsushima, K et al. Nat. Commun. 7: 13616, 2016); (Deguchi, S et al. Oncogene. 36 (32) : 4629 - 4640, 2017). RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
          Ordering Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
          Order form 
          [Credit Card Payment]  [Bank Transfer Payment]
          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          Approval form by depositor [Word]
          Remarks ((Additional form)) Approval Form(FormD)
          Please obtain written approval of the depositor.
          提供案内 (日本国内) [open/close]

          提供条件 利用者は提供承諾書を用いて、事前に寄託者の承諾を得る。利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Katsushima, K et al. Nat. Commun. 7: 13616, 2016); (Deguchi, S et al. Oncogene. 36 (32) : 4629 - 4640, 2017)。利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び事業活動を行う場合は、寄託者と別途協議を行う。営利機関の利用希望者は、事前に利用条件等につき寄託者と合意し、提供承諾を得ること。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          寄託者による提供承諾書 [Word]
          備考 【追加提供依頼書】承諾書(FormD)
          寄託者の書面による提供承諾が必要です。

          Catalog # Resource name Availability Shipping form Fee (non-profit org.)
          RDB15798 p3xFLAG-Myc-CMV-AGO2-PAZ-mut Under QC test. Please contact us. DNA solution

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          How to cite this biological resource

          Materials & Methods section:

          The p3xFLAG-Myc-CMV-AGO2-PAZ-mut was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15798).

          Reference section:

          Deguchi, S., Katsushima, K., Hatanaka, A., Shinjo, K., Ohka, F., Wakabayashi, T., Zong, H., Natsume, A., Kondo, Y., Oncogenic effects of evolutionarily conserved noncoding RNA ECONEXIN on gliomagenesis. Oncogene 36 (32): 4629-4640 (2017). PMID 28368417.

          Further references such as user reports and related articles (go to bottom)


          References and tips

          Featured content


          QC test results

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.


          References

          Original, user report and related articles

          original Deguchi, S., Oncogenic effects of evolutionarily conserved noncoding RNA ECONEXIN on gliomagenesis. Oncogene 36 (32): 4629-4640 (2017). PMID 28368417.

          2023.05.22

          GNP_filter3_RDBDEP_html_230512.pl