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pGEX-6p-2-3MST (#RDB15868)

E. coli expression vector of mouse 3-mercaptopyruvate sulfurtransferase (3MST).


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Publication Kimura, Y., Sci. Rep. 7 (1): 10459 (2017). PMID 28874874. [PubMed] [Article] [RRC of NBRP]
Test sheet Data Sheet open 
 
Clone info. E. coli expression vector of mouse 3-mercaptopyruvate sulfurtransferase (3MST, Mpst) fused with GST at N-terminal.
Comment Note: nucleotide substitution in mouse Mpst (A to G in 499nt of NM_138670.3 (Asp to Gly)), which has been noticed by the depositor.
Vector backbone pGEX-6P-2 (Plasmid)
Size of vector backbone 5.0 kb
Selectable markers Amp^r
Gene/insert name Mouse Mpst cDNA
Depositor|Developer Kimura, Hideo |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and a citation of the following literature designated by the DEPOSITOR are requested (Kimura, Y et al. Sci. Rep. 7(1), 10459, 2017). Any commercial use of the BIOLOGICAL RESOURCE requires prior written consent of the DEPOSITOR.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
提供同意書 (MTA, 営利目的用)[Word]
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Kimura, Y et al. Sci Rep. 7(1), 10459, 2017)を引用し、謝辞の表明を必要とする。営利機関の利用者もしくは商業利用を目的とする利用者は、事前にその旨を寄託者に届出し、また提供承諾書を取得することとする。

Catalog # Resource name Shipping form Fee
RDB15868 pGEX-6p-2-3MST DNA solution JPY 9,460 (not-for-profit academic purpose)
JPY 18,920 (for-profit-research purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

How to cite this biological resource

Materials & Methods section:

The pGEX-6p-2-3MST was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15868).

Reference section:

Kimura, Y., Koike, S., Shibuya, N., Lefer, D., Ogasawara, Y., Kimura, H., 3-Mercaptopyruvate sulfurtransferase produces potential redox regulators cysteine- and glutathione-persulfide (Cys-SSH and GSSH) together with signaling molecules H2S2, H2S3 and H2S. Sci. Rep. 7 (1): 10459 (2017). PMID 28874874. [PubMed] [Article] [RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Kimura, Y., 3-Mercaptopyruvate sulfurtransferase produces potential redox regulators cysteine- and glutathione-persulfide (Cys-SSH and GSSH) together with signaling molecules H2S2, H2S3 and H2S. Sci. Rep. 7 (1): 10459 (2017). PMID 28874874. [PubMed] [Article] [RRC of NBRP]