RDB15882 - pGEX6P-3-SUV39H1(39-105)-Y67A

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pGEX6P-3-SUV39H1(39-105)-Y67A

Expression vector of mouse SUV39H1, chromodomain (residues 39-105), methylated H3K9 binding mutant (Y67A).

Catalog number	RDB15882
Resource name	pGEX6P-3-SUV39H1(39-105)-Y67A
Clone info.	Expression vector of mouse suppressor of variegation 3-9 homolog 1 (SUV39H1), chromodomain (CD, residues 39-105), methylated H3K9 binding mutant (Y67A) fused with N-terminal GST, tac promoter, see Fig. 1A and Fig. 2A of Shirai, A. et al., Elife, 1; 6. pii: e25317, 2017.
Comment	Expression was confirmed by the depositor with CBB staining and Western blot.
Vector backbone	pGEX-6P-3 (plasmid)
Size of vector backbone	5.0 kb
Selectable markers	Amp^r
Gene/insert name	mouse Suv39h1 cDNA
Depositor\|Developer	Shinkai, Yoichi \|

Other clones in our bank

mouse Suv39h1

External Database
mouse Suv39h1

Reference sequence

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Shirai, A et al. ELIFE. (6), e25317, 2017)

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Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Shirai, A et al. ELIFE. (6), e25317, 2017)
Ordering	Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. Order form [Credit Card Payment] [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Shirai, A et al. ELIFE. (6), e25317, 2017)。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB15882	pGEX6P-3-SUV39H1(39-105)-Y67A	DNA solution

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original	Shirai, A., Impact of nucleic acid and methylated H3K9 binding activities of Suv39h1 on its heterochromatin assembly. Elife 6. pii: e25317 (2017). PMID 28760201.

Further references such as user reports and related articles (go to bottom)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB15882_A8Dgp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pGEX_F2 Sequence file: RDB15882_A8Dga.seq
>D05294A3-A8Dg__1_pGEX_F2_H02_08_ABI08.ab1 1 CAATTAGTTT TGGTTTTTAA AAAACGTATT GAAGCTATCC CACAAATTGA TAAGTACTTG 61 AAATCCAGCA AGTATATAGC ATGGCCTTTG CAGGGCTGGC AAGCCACGTT TGGTGGTGGC 121 GACCATCCTC CAAAATCGGA TCTGGAAGTT CTGTTCCAGG GGCCCCTGGG ATCCAATCTG 181 TATGACTTTG AAGTTGAATA CCTGTGTGAT TATAAGAAGA TCCGTGAGCA GGAGTATTAC 241 CTGGTTAAGT GGCGTGGGGC TCCCGACTCA GAAAACACCT GGGAGCCACG GCAGAATCTA 301 AAATGTATAC GAGTTCTTAA GCAGTTCCAC AAGGACTTAG AAAGAGAGCT TGTCCGACGA 361 CACCGCCGGT CAAAGTGAGT CGACTCGAGC GGCCGCATCG TGACTGACTG ACGATCTGCC 421 TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG GAGACGGTCA 481 CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG TCAGCGGGTG 541 TTGGCGGGTG TCGGGGCGCA GCCATGACCC AGTCACGTAG CGATAGCGGA GTGTATAATT 601 CTTGAAGACG AAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA 661 TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT 721 TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC 781 TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC 841 CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA 901 AAGATGCTGA AGATCAGTTG GGGTGCACGA GTGGGTTACA TCGAACTGGG ATCTCAACAG 961 CGGTAAGATC CTTGGAGAGT TTTCGCCCCG AAGAAACGTT TTCCAATGAT GAGCACTTTT 1021 TAAAGGTTCT GCCTATGTGG CGCGGTATTA TCCCGTGATG ACGCCGGGCA AGAGCAACTT 1081 CGGTCGCGGC ATACACTATT CTCAGAATGA ACTGGGTTTG AGTACCTCCC AGTCACAAGA 1141 AGAGGCACTC TACGGATGGC ATGACAGGTT AAGGAGAGTC A //
Primer: pGEX-ptac_up-F Sequence file: RDB15882_A8Dgb.seq
>D05294A3-1_A8Dg_pGEX-ptac_up-F_E01_05_ABI08.ab1 1 TTGGAAGGGG ACATCATAAC GGTTCTGGCA ATATTCTGAA ATGAGGCTGT TGACAATTAA 61 TCATCGGCTC GTATAATGTG TGGAATTGTG AGCGGATAAC AATTTCACAC AGGAAACAGT 121 ATTCATGTCC CCTATACTAG GTTATTGGAA AATTAAGGGC CTTGTGCAAC CCACTCGACT 181 TCTTTTGGAA TATCTTGAAG AAAAATATGA AGAGCATTTG TATGAGCGCG ATGAAGGTGA 241 TAAATGGCGA AACAAAAAGT TTGAATTGGG TTTGGAGTTT CCCAATCTTC CTTATTATAT 301 TGATGGTGAT GTTAAATTAA CACAGTCTAT GGCCATCATA CGTTATATAG CTGACAAGCA 361 CAACATGTTG GGTGGTTGTC CAAAAGAGCG TGCAGAGATT TCAATGCTTG AAGGAGCGGT 421 TTTGGATATT AGATACGGTG TTTCGAGAAT TGCATATAGT AAAGACTTTG AAACTCTCAA 481 AGTTGATTTT CTTAGCAAGC TACCTGAAAT GCTGAAAATG TTCGAAGATC GTTTATGTCA 541 TAAAACATAT TTAAATGGTG ATCATGTAAC CCATCCTGAC TTCATGTTGT ATGACGCTCT 601 TGATGTTGTT TTATACATGG ACCCAATGTG CCTGGATGCG TTCCCAAAAT TAGTTTGTTT 661 TAAAAAACGT ATTGAAGCTA TCCCACAAAT TGATAAGTAC TTGAAATCCA GCAAGTATAT 721 AGCATGGCCT TTGCAGGGCT GGCAAGCCAC GTTTGGTGGT GGCGACCATC CTCCAAAATC 781 GGATCTGGAA GTTCTGTTCC AGGGGCCCCT GGGATCCAAT CTGTATGACT TTGAAGTTGA 841 ATACCTGTGT GATTATAAGA AAGATCCGTG AGCAGGAGTA TTACCTGGTT AAGTGGCGTG 901 GGGGCTCCCG ACTCAGAAAA CACCTGGGGA GCCACGGGCA GAATCTAAAA ATGTATACGG 961 AGTTTCTTAA AGCAGTTTCC ACAAAGGGAC TTTAGAAAGG AGAAGCTTTG TTCCGGACGA 1021 CACCGGCCGG GTCAAAGTGA AGTCGGACTC GAGCGGGCCC GCATCGGTGG ACTGACTGAC 1081 GATCTGGCCT TCGCGCGATT CGGTGAATGG AACGGTGAAA AACCCTTCTG ACACACAATG 1141 CCAGGCT //
Primer: SV40pA3' element Reverse Sequence file: RDB15882_A8Dgc.seq
>D05294A3-1_A8Dg_SV40pA3'elementReverse_F01_06_ABI08.ab1 1 TCTTATGGGG GGGGGGGGGG GCTATGGGGA AAACCGCCAG CAACGGCGGG CCTTTTTACG 61 GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC 121 TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC 181 CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CTGATGCGGT ATTTTCTCCT 241 TACGCATCTG TGCGGTATTT CACACCGCAT AAATTCCGAC ACCATCGAAT GGCGCAAAAC 301 CTTTCGCGGT ATGGCATGAT AGCGCCCGGA AGAGAGTCAA TTCAGGGTGG TGAATGTGAA 361 ACCAGTAACG TTATACGATG TCGCAGAGTA TGCCGGTGTC TCTTATCAGA CCGTTTCCCG 421 CGTGGTGAAC CAGGCCAGCC ACGTTTCTGC GAAAACGCGG GAAAAAGTGG AAGCGGCGAT 481 GGCGGAGCTG AATTACATTC CCAACCGCGT GGCACAACAA CTGGCGGGCA AACAGTCGTT 541 GCTGATTGGC GTTGCCACCT CCAGTCTGGC CCTGCACGCG CCGTCGCAAA TTGTCGCGGC 601 GATTAAATCT CGCGCCGATC AACTGGGTGC CAGCGTGGTG GTGTCGATGG TAGAACGAAG 661 CGGCGTCGAA GCCTGTAAAG CGGCGGTGCA CAATCTTCTC GCGCAACGCG TCAGTGGGCT 721 GATCATTAAC TATCCGCTGG ATGACCAGGA TGCCATTGCT GTGGAAGCTG CCTGCACTAA 781 TGTTCCGGCG TTATTTCTTG ATGTCTCTGA CCAGACACCC ATCAACAGTA TTATTTTCTC 841 CCATGAAGAC GGTACGCGAC TGGGGCGTGG AGCATCTGGG TCGCATTTGG GTCACCCAGC 901 CAAATTCGCG GCTTGTTTAG CCGGGGCCTC AATTAAAGTT TCTGGTCTCG GGCGCGTTCT 961 GGCGTTCTGG GCTGGGCTGG GGCATTAAAA TATTCTCAAC TCCGCCAAAT CCAAAATTTT 1021 CAGGCCCGAA TTAGCCGGGG AACCGGGGAA AGGGGCCGGC CTTGGGAAGT GGCCCATGAT 1081 TCCCGGGGTT TTTCCAGCAA ACCCCAATTG GCCAAATTGC TGGGAACTGC AAGGGGGGCA 1141 ATTCGATTTT CCTCCCACCA CCTCTTGGCC GCG //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Shirai, A., Impact of nucleic acid and methylated H3K9 binding activities of Suv39h1 on its heterochromatin assembly. Elife 6. pii: e25317 (2017). PMID 28760201.

2022.05.17

GNP_filter3_RDBDEP_html_220516.pl