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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pX330-Syk gRNA

Targeting construct of human spleen tyrosine kinase (Syk).

Catalog number RDB15904
Resource name pX330-Syk gRNA
Clone info. Expression vector of gRNA for targeting human spleen tyrosine kinase (Syk) and human codon-optimized SpCas9.
Comment Effect of the targeting vecctor was confirmed by the depositor with immunoblotting.
Vector backbone pX330 (plasmid)
Size of vector backbone 8.5 kb
Selectable markers Amp^r
Gene/insert name Human SYK genomic DNA
Depositor|Developer Sada, Kiyonao |
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External Database
Human SYK

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Chihara, K et al. Sci Rep. 7(1) : 11480, 2017)
          Ordering Please visit Information of Request for Distribution.[link] 
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          Exclusive MTA (For the DNA materials containing CRISPR/Cas9 technologies and for not-for-profit academic purpose) [Word]
          Remarks
          The BIOLOGICAL RESOURCE contains CRISPR/Cas9 technologies and is not provided to for-profit organization or for for-profit research by non-profit organizations.
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Chihara, K et al. Sci Rep. 7(1) : 11480, 2017)。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          専用MTA(CRISPR/Cas9内包遺伝子材料専用 非営利学術目的)をお使いください [Word]
          備考
          本件リソースはCRISPR/Cas9 technologyを用いたゲノム編集バイオリソースです。営利機関および非営利機関による営利目的研究には提供いたしません。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB15904 pX330-Syk gRNA DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Chihara, K., Syk-dependent tyrosine phosphorylation of 3BP2 is required for optimal FcRγ-mediated phagocytosis and chemokine expression in U937 cells. Sci. Rep. 7 (1): 11480 (2017). PMID 28904407.

          Further references such as user reports and related articles (go to bottom)

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          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB15904_A8Crp1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: SV40pA3' element Reverse
          Sequence file: RDB15904_A8Cra.seq check
          >D05305A2-1_A8Cr_40pA3'elementRever_D01_10_ABI24.ab1
              1 TTTTAGGTGG GCGGACCTAT GGAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT
             61 TTTGCTGGCC TTTTGCTCAC ATGTGAGGGC CTATTTCCCA TGATTCCTTC ATATTTGCAT
            121 ATACGATACA AGGCTGTTAG AGAGATAATT GGAATTAATT TGACTGTAAA CACAAAGATA
            181 TTAGTACAAA ATACGTGACG TAGAAAGTAA TAATTTCTTG GGTAGTTTGC AGTTTTAAAA
            241 TTATGTTTTA AAATGGACTA TCATATGCTT ACCGTAACTT GAAAGTATTT CGATTTCTTG
            301 GCTTTATATA TCTTGTGGAA AGGACGAAAC ACCGTTTCGG CAACATCACC CGGGGTTTTA
            361 GAGCTAGAAA TAGCAAGTTA AAATAAGGCT AGTCCGTTAT CAACTTGAAA AAGTGGCACC
            421 GAGTCGGTGC TTTTTTGTTT TAGAGCTAGA AATAGCAAGT TAAAATAAGG CTAGTCCGTT
            481 TTTAGCGCGT GCGCCAATTC TGCAGACAAA TGGCTCTAGA GGTACCCGTT ACATAACTTA
            541 CGGTAAATGG CCCGCCTGGC TGACCGCCCA ACGACCCCCG CCCATTGACG TCAATAGTAA
            601 CGCCAATAGG GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT
            661 TGGCAGTACA TCAAGTGTAT CATATGCCAA GTACGCCCCC TATTGACGTC AATGACGGTA
            721 AATGGCCCGC CTGGCATTGT GCCCAGTACA TGACCTTATG GGACTTTCCT ACTTGGCAGT
            781 ACATCTACGT ATTAGTCATC GCTATTACCA TGGTCGAGGT GAGCCCCCAC GTTCTGCTTC
            841 ACTCTCCCCA TCTCCCCCCC CCCTCCCCAC CCCCCAATTT TTGTATTTTA TTTTATTTTT
          901 TTTAATTTAT TTTTGTGCAG CCGATGGGGG GCGGGGGGGG GGGGGGGACG
          //
          Primer: RDB13535#1
          Sequence file: RDB15904_A8Crb.seq check
          >D05305A2-1_A8Cr_RDB13535#1_E01_13_ABI24.ab1
              1 TGACAAGGGC CTGAATCACT TTTTTTCAGG TTGGACCGGT GCCACCATGG ACTATAAGGA
             61 CCACGACGGA GCACTACAAG GATCATGATA TTGATTACAA AGACGATGAC GATAAGATGG
            121 CCCCAAAGAA GAAGCGGAAG GTCGGTATCC ACGGAGTCCC AGCAGCCGAC AAGAAGTACA
            181 GCATCGGCCT GGACATCGGC ACCAACTCTG TGGGCTGGGC CGTGATCACC GACGAGTACA
            241 AGGTGCCCAG CAAGAAATTC AAGGTGCTGG GCAACACCGA CCGGCACAGC ATCAAGAAGA
            301 ACCTGATCGG AGCCCTGCTG TTCGACAGCG GCGAAACAGC CGAGGCCACC CGGCTGAAGA
            361 GAACCGCCAG AAGAAGATAC ACCAGACGGA AGAACCGGAT CTGCTATCTG CAAGAGATCT
            421 TCAGCAACGA GATGGCCAAG GTGGACGACA GCTTCTTCCA CAGACTGGAA GAGTCCTTCC
            481 TGGTGGAAGA GGATAAGAAG CACGAGCGGC ACCCCATCTT CGGCAACATC GTGGACGAGG
            541 TGGCCTACCA CGAGAAGTAC CCCACCATCT ACCACCTGAG AAAGAAACTG GTGGACAGCA
            601 CCGACAGGCC GACCTGCGGC TGATCTATCT GGCCCTGGCC CACATGATCA AGTTCCGGGG
            661 CCACTTCCTG ATCGAGGGCG ACCTGAACCC CGACAACAGC GACGTGGACA AGCTGTTCAT
            721 CCAGCTGGTG CAGACCTACA ACCAGCTGTT CGAGGAAAAC CCCATCAACG CCAGCGGCGT
            781 GGACGCCCAA GGCCATCCTG TCTGCCAGAC TGAGCAAGAG CAGACGGCTG GGAAAATCTG
            841 ATCGGCCCAG CTGCCCGCCG AGAAGAAGAA TGGCCCTGTT CGGAAACCTG ATTGCCTGAG
            901 CCTGGGCCTG GACCCCCAAC TTCAGAACAC TTCGACCTGG TCGAGGATGC CAACCTGCAG
            961 CTGAGCAAGA CACCCTTACG AACGAACGAC TGGAACACCT GGCTGGCCGA ATCCGCGACA
          1021 GATACGCGAA CTGTTCTGGC CGCAGACTGT CGAGTCATCC TGGCTGAACG G
          //
          Primer: BGH_rev
          Sequence file: RDB15904_A8Crc.seq check
          >D05305A2-1_A8Cr_BGH_rev2_F01_16_ABI24.ab1
              1 TATGGGTACA GCACGGGGGA GGGGCAAACA ACAGATGGCT GGCAACTAGA AGGCACAGTC
             61 GAGGCTGATC AGCGAGCTCT AGGAATTCTT ACTTTTTCTT TTTTGCCTGG CCGGCCTTTT
            121 TCGTGGCCGC CGGCCTTTTG TCGCCTCCCA GCTGAGACAG GTCGATCCGT GTCTCGTACA
            181 GGCCGGTGAT GCTCTGGTGG ATCAGGGTGG CGTCCAGCAC CTCTTTGGTG CTGGTGTACC
            241 TCTTCCGGTC GATGGTGGTG TCAAAGTACT TGAAGGCGGC AGGGGCTCCC AGATTGGTCA
            301 GGGTAAACAG GTGGATGATA TTCTCGGCCT GCTCTCTGAT GGGCTTATCC CGGTGCTTGT
            361 TGTAGGCGGA CAGCACTTTG TCCAGATTAG CGTCGGCCAG GATCACTCTC TTGGAGAACT
            421 CGCTGATCTG CTCGATGATC TCGTCCAGGT AGTGCTTGTG CTGTTCCACA AACAGCTGTT
            481 TCTGCTCATT ATCCTCGGGG GAGCCCTTCA GCTTCTCATA GTGGCTGGCC AGGTACAGGA
            541 AGTTCACATA TTTGGAGGGC AGGGCCAGTT CGTTTCCCTT CTGCAGTTCG CCGGCAGAGG
            601 CCAGCATTCT CTTCCGGCCG TTTTCCAGCT CGAACAGGGA GTACTTAGGC AGCTTGATGA
            661 TCAGGTCCTT TTTCACTTCT TTGTAGCCCT TGGCTTCCAG AAAGTCGATG GGATTCTTCT
            721 CGAAGCTGCT TCTTTCCATG ATGGTGATCC CCAGCAGCTC TTTCACACTC TTCAGTTTCT
            781 TGGACTTGCC CTTTTCCACT TTGGCCACCA CCAGCACAGA ATAGGCCACG GTGGGGCTGT
            841 CGAAGCCGCC GTACTTCTTA GGGTCCCAGT CCTTCTTTCT GGCGATCAGC TTATCGCTGT
            901 TCCTCTTGGG CAGGATAGAC TCTTTGCTGA AGCCGCCCTG TCTGCACCCT CGGTCTTTTT
            961 TCACGATATT CACTTTGGGG GCATGCTCAG CACTTTCCGC CACGGTGCAA AATCCCGGCT
          1021 GATATCCCAC ACGATCTCCC TGAATTCGAC CGATTTCTCG AATCAGAGGA CGCCT
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Chihara, K., Syk-dependent tyrosine phosphorylation of 3BP2 is required for optimal FcRγ-mediated phagocytosis and chemokine expression in U937 cells. Sci. Rep. 7 (1): 11480 (2017). PMID 28904407.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl