Please review the QC test results indicated by icon below as well as clone information before placing your order.

pX330-Hck gRNA

Targeting construct of human hematopoietic cell kinase (Hck).

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Chihara, K et al. Sci Rep. 7(1) : 11480, 2017)
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Exclusive MTA (For the DNA materials containing CRISPR/Cas9 technologies and for not-for-profit academic purpose) [Word]
Remarks	The BIOLOGICAL RESOURCE contains CRISPR/Cas9 technologies and is not provided to for-profit organization or for for-profit research by non-profit organizations.

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Chihara, K et al. Sci Rep. 7(1) : 11480, 2017)。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 専用MTA（CRISPR/Cas9内包遺伝子材料専用 非営利学術目的）をお使いください [Word]
備考	本件リソースはCRISPR/Cas9 technologyを用いたゲノム編集バイオリソースです。営利機関および非営利機関による営利目的研究には提供いたしません。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB15905_A8Crp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: SV40pA3' element Reverse Sequence file: RDB15905_A8Cra.seq
>D05305A3-2_A8Cr_40pA3'elementRever_G01_19_ABI24.ab1     1 GACAGGAGGC GGAGCCTATG GAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT    61 TTTGCTGGCC TTTTGCTCAC ATGTGAGGGC CTATTTCCCA TGATTCCTTC ATATTTGCAT   121 ATACGATACA AGGCTGTTAG AGAGATAATT GGAATTAATT TGACTGTAAA CACAAAGATA   181 TTAGTACAAA ATACGTGACG TAGAAAGTAA TAATTTCTTG GGTAGTTTGC AGTTTTAAAA   241 TTATGTTTTA AAATGGACTA TCATATGCTT ACCGTAACTT GAAAGTATTT CGATTTCTTG   301 GCTTTATATA TCTTGTGGAA AGGACGAAAC ACCGTGGGAT CCGGCACGTA CACGTTTTAG   361 AGCTAGAAAT AGCAAGTTAA AATAAGGCTA GTCCGTTATC AACTTGAAAA AGTGGCACCG   421 AGTCGGTGCT TTTTTGTTTT AGAGCTAGAA ATAGCAAGTT AAAATAAGGC TAGTCCGTTT   481 TTAGCGCGTG CGCCAATTCT GCAGACAAAT GGCTCTAGAG GTACCCGTTA CATAACTTAC   541 GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC CCATTGACGT CAATAGTAAC   601 GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT TTACGGTAAA CTGCCCACTT   661 GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT ATTGACGTCA ATGACGGTAA   721 ATGGCCCGCC TGGCATTGTG CCCAGTACAT GACCTTATGG GACTTTCCTA CTTGGCAGTA   781 CATCTACGTA TTAGTCATCG CTATTACCAT GGTCGAGGTG AGCCCCACGT TCTGCTTCAC   841 TCTCCCCATC TCCCCCCCCC CTCCCCACCC CCCAATTTTG TATTTATTTT ATTTTTTTTA 901 ATTTATTTTT GTGCAGCGAT GGGGGGCGCG GGGGGGGGG //
Primer: RDB13535#1 Sequence file: RDB15905_A8Crb.seq
>D05305A3-2_A8Cr_RDB13535#1_H01_22_ABI24.ab1     1 TTGAACATGA CCTGAATCAC TTTTTTTCAG GTTGGACCGG TGCCACCATG GACTATAAGG    61 ACCACGACGG AGACTACAAG GATCATGATA TTGATTACAA AGACGATGAC GATAAGATGG   121 CCCCAAAGAA GAAGCGGAAG GTCGGTATCC ACGGAGTCCC AGCAGCCGAC AAGAAGTACA   181 GCATCGGCCT GGACATCGGC ACCAACTCTG TGGGCTGGGC CGTGATCACC GACGAGTACA   241 AGGTGCCCAG CAAGAAATTC AAGGTGCTGG GCAACACCGA CCGGCACAGC ATCAAGAAGA   301 ACCTGATCGG AGCCCTGCTG TTCGACAGCG GCGAAACAGC CGAGGCCACC CGGCTGAAGA   361 GAACCGCCAG AAGAAGATAC ACCAGACGGA AGAACCGGAT CTGCTATCTG CAAGAGATCT   421 TCAGCAACGA GATGGCCAAG GTGGACGACA GCTTCTTCCA CAGACTGGAA GAGTCCTTCC   481 TGGTGGAAGA GGATAAGAAG CACGAGCGGC ACCCCATCTT CGGCAACATC GTGGACGAGG   541 TGGCCTACCA CGAGAAGTAC CCCACCATCT ACCACCTGAG AAAGAAACTG GTGGACAGCA   601 CCGACAAGGC CGACCTGCGG CTGATCTATC TGGCCCTGGC CCACATGATC AAGTTCCGGG   661 GCCACTTCCT GATCGAGGGC GACCTGAACC CCGACAACAG CGACGTGGAC AAGCTGTTCA   721 TCCAGCTGGT GCAGACCTAC AACCAGCTGT TCGAGGAAAA CCCCATCAAC GCCAGCGGCG   781 TGGACGCCAA GGCCATCCTG TCTGCCAGAC TGAGCAAGAG CAGACGGCTG GGAAAATCTG   841 ATCGCCCAGC TGCCCGGCGA GAAGAAGAAT GGCCTGTTTC GGAAACCTGA TTGCCCTGAG   901 CCCTGGGCCT GACCCCCAAC TTTCAAGAGC AACTTTCGAC CTGTCGGAGG ATGGCCAAAC   961 TGCAGCTGAG CAGGACACCT ACCGAACGAC CGACTGACAC TGCTTGGTCC AGATCCGCGA  1021 CAGTACGCGA ACTGGTTTCT GCGCAGGACT GGTCGGACGC CATCTGGCTG ACGACATTCC 1081 GTG //
Primer: BGH_rev Sequence file: RDB15905_A8Crc.seq
>D05305A3-2_A8Cr_BGH_rev2_A02_02_ABI24.ab1     1 GTTGGGGGCC AGGAGGCACG GGGGAGGGGC AAACAACAGA TGGCTGGCAA CTAGAAGGCA    61 CAGTCGAGGC TGATCAGCGA GCTCTAGGAA TTCTTACTTT TTCTTTTTTG CCTGGCCGGC   121 CTTTTTCGTG GCCGCCGGCC TTTTGTCGCC TCCCAGCTGA GACAGGTCGA TCCGTGTCTC   181 GTACAGGCCG GTGATGCTCT GGTGGATCAG GGTGGCGTCC AGCACCTCTT TGGTGCTGGT   241 GTACCTCTTC CGGTCGATGG TGGTGTCAAA GTACTTGAAG GCGGCAGGGG CTCCCAGATT   301 GGTCAGGGTA AACAGGTGGA TGATATTCTC GGCCTGCTCT CTGATGGGCT TATCCCGGTG   361 CTTGTTGTAG GCGGACAGCA CTTTGTCCAG ATTAGCGTCG GCCAGGATCA CTCTCTTGGA   421 GAACTCGCTG ATCTGCTCGA TGATCTCGTC CAGGTAGTGC TTGTGCTGTT CCACAAACAG   481 CTGTTTCTGC TCATTATCCT CGGGGGAGCC CTTCAGCTTC TCATAGTGGC TGGCCAGGTA   541 CAGGAAGTTC ACATATTTGG AGGGCAGGGC CAGTTCGTTT CCCTTCTGCA GTTCGCCGGC   601 AGAGGCCAGC ATTCTCTTCC GGCCGTTTTC CAGCTCGAAC AGGGAGTACT TAGGCAGCTT   661 GATGATCAGG TCCTTTTTCA CTTCTTTGTA GCCCTTGGCT TCCAGAAAGT CGATGGGATT   721 CTTCTCGAAG CTGCTTCTTT CCATGATGGT GATCCCCAGC AGCTCTTTCA CACTCTTCAG   781 TTTCTTGGAC TTGCCCTTTT CCACTTTGGC CACCACCAGC ACAGAATAGG CCACGGGTGG   841 GGCTGTCGAA GCCGCCGTAC TTCTTAGGGT CCCAGTCCTT CTTTCTGGCG ATCAGCTTAT   901 CGCTGTTTCC TCTTGGGCAG GATAGACTCT TTGCTGAAGC CCGCCTGTCT GCACCTCGGT   961 CTTTTTTCAC GATATTCACT TTGGGGCATG CTCAGCACTT TCCGGCACGG TGGCCAAAAT  1021 CCCCGGCCCT TAATCCACAC GAATCTCCCC CGAATTCGTC GATTGTCTCG ATCAGAGGTC 1081 GCATCCTGGA ATTC //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.05.17

GNP_filter3_RDBDEP_html_220516.pl