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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pET-pfu-FAU-1-His

Bacterial expression vector of P. furiosus AU binding 1 FAU-1.

Catalog number RDB15910
Resource name pET-pfu-FAU-1-His
Clone info. Bacterial expression vector of P. furiosus AU binding 1 (FAU-1) tagged with 6xHis at C terminal. For the constitution and cloning, see Fig.5A of Ikeda, Y. et al., Sci. Rep., 7 (1):12674, 2017, and Kanai Aet al., Biochem. J. 372 (Pt 1): 253-261. 2003, respectively.
Comment Expression was confirmed by the depositor with SDS/PAGE. See also the following paper: Expression cloning and characterization of a novel gene that encodes the RNA-binding protein FAU-1 from Pyrococcus furiosus. Kanai A, Oida H, Matsuura N, Doi H. Biochem J. 372(Pt 1):253-261. 2003.
Vector backbone pET23b (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers kan^r
Gene/insert name P. furiosus FAU-1 genomic DNA
Reference of insert sequence AB055587 (DDBJ accession number)
Depositor|Developer Kanai, Akio |
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External Database
P. furiosus FAU-1
          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Ikeda, Y et al. Biochemical Journal 372: 253-261, 2003)
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Ikeda, Y et al. Biochemical Journal 372: 253-261, 2003)。
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          RDB15910 pET-pfu-FAU-1-His DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Ikeda, Y., An archaeal RNA binding protein, FAU-1, is a novel ribonuclease related to rRNA stability in Pyrococcus and Thermococcus. Sci. Rep. 7 (1): 12674 (2017). PMID 28978920.

          Further references such as user reports and related articles (go to bottom)

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          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB15910_A8Dnp1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: T7 terminator
          Sequence file: RDB15910_A8Dna.seq check
          >D05496A1-5_A8Dn_T7terminater_H01_22_ABI24.ab1
              1 AATTAACCTT CCTTTTCGGG CTTTGTTAGC AGCCGGATCT CAGTGGTGGT GGTGGTGGTG
             61 CTCGAGCCTG TCGTAGACCT CCTGGGCAAT CCTCAGCGTT GCCTTATAGA GCTTCTCACT
            121 AATAATTCCC TCCTCGTAGT GTTCCTTTAG CTTCTCCTTA TCTATGATCT CCTTCTTTCC
            181 GTCTGGCCAC CTCACTATGT CGACCTCTAG ATCCACATAT CTTGCCTTAT CTGGATAGAT
            241 CTCCACAGGG GTGTTGATGT TGTAGAACTC TCCCTTAAGG TTGCCATCTT TATCATAGTA
            301 TCTGTGAACA AACCACCACT TTCCAGCCTC TATTTCAGTT ATTGCGTAAT CTCCACTCTC
            361 TATTGGCACT TCCAGTCCAT CATAGAACTT TCCTGGCCTT AGATATCTCT TTATTGTCAC
            421 TTTTAAAGGA TCCGTGGATA CCTCTATTAC TTCCCCTGGG CCAATCTTTA TTATCTGCCC
            481 ATCAGGTTTC ACGTGGTTCA GCGTGAATAT CCAACCAACC TTTGGTCCTT TACTGGTAAT
            541 TATAGCTTCT AGAAATCCTT TACTTATCTT CTGCCTCTGG GACGGGAGTT TTGCCAGAAT
            601 CCCCTCTGCG ACATCTACAG CTAATGTAAA CTCTGGGTCG TAGGACTTGA ACTGGTGGTG
            661 CCCTTCAATT GTGGGAACAA CTTCATTCCT AATCTCATCA AGCTTTTTCT TGACTCCACC
            721 ACCGAATTCA ATCTCGTAAA TTTCCCTCCC CTCTATTATC TCTGCTGGAG CTGAGAACTT
            781 CTCTGCCTCC TTCAGCTTAT CTGCAATTTT TGAAAGCCTC ACTAGTTCAT CCCTTAACGT
            841 GTTCCAATCC TTGTAAGCGG CAGCAGTTCT CCAGAGAACA CCCCACTCTC CAAGATCAAC
            901 ACTTAGTCCC AGGATTCTCA ACCTTTCCCT TTCCTCGGAT CCTTTATTCT TTTCTGGGAA
            961 ATCTTCACGT GTCTCTGAAC TTCCTAATGG CTATGGAAAT CAGCACTGCA TAAGTCACCC
           1021 GGGAATTGTT ATTAAGTTGC TTAAGGTGTT GGGGAGGACG TTGTGCTTCT TCACTTTGGA
           1081 CAACCAACTT TCAATCCTTC GGCCAGCATT CTGGAAGGCT TCTCTATGGA GACGTTCGAT
          1141 AACATTCCGA TATATCTACA TAACCAAAGC G
          //
          Primer: T7_20bp
          Sequence file: RDB15910_A8Dnb.seq check
          >D05496A1-5_A8Dn_T7_20bp_A02_02_ABI24.ab1
              1 GGAAATTTCA TCTAGAATAT TTTGTTTACT TTAAGAAGGA GATATACATA CATATGGTGA
             61 AAGTGTCTAC AGAATCAGAA ATAGCGGTGA GGATAAGGGG AATATATAGC ACGGCCCTAA
            121 CAAAGCTCCT AATGGACAGG GGTTTCAAGA TAGTACAGCC AAGCGACGTG ATAGCTGAGA
            181 GGTTTGGAAT TGAGAAGAGC TATGAGGACT TTGACGTCGA CATCTACGAC AAAAACCACG
            241 GTGTGACAAT CGTTGGAACC AAAGTGGAGG CGGTGAAGAA GGTCTTTGAG GAGGAGTTTA
            301 TTGACGTCTT TTTTAGAAAG CTCCCCTATA AGCTCCATGG AATTTACAAG GGGCTTGTGG
            361 TGAAAAGAGA TGACAGGTTT GTTTATGTAG ATATTGGGAA TGTTATTGGA ACGGTTCTCA
            421 TAGAGGAGCT TCCAGATGCT GCCGAAGGGG ATGAAGTTGT TGTTCAAGTG AAGAAGCACA
            481 ACGTCCTCCC ACACCTAAGC ACCTTAATAA CAATCCCGGG TGACTATGCA GTGCTGATTC
            541 CAAAGCCCAT AGGAGTTCAG AGACACGTGA AGATTTCCAG AAAGATAAAG GATCCCGAGG
            601 AAAGGGAAAG GTTGAGAATC CTGGGACTAA GTGTTGATCT TGGAGAGTGG GGTGTTCTCT
            661 GGAGAACTGC TGCCGCTTAC AAGGATTGGA ACACGTTAAG GGATGAACTA GTGAGGCTTT
            721 CAAAAATTGC AGATAAGCTG AAGGAGGCAG AGAAGTTCTC AGCTCCAGCA GAGATAATAG
            781 AGGGGAGGGA AATTTACGAG ATTGAATTCG GTGGTGGAGT CAAGAAAAAG CTTGATGAGA
            841 TTAGGAATGA AGTTGTTCCC ACAATTGAAG GGCACCACCA GTTCAAGTCC TACGACCCAG
            901 AGTTTACATT AGCTGTAGAT GTCGCAGAGG GGATTCTGGC AAAACTCCCG TCCCAGAGGC
            961 AGAAGATAAG TAAAGGATTT CTAGAAGCTA TAATTACCCA GTAAAGGACC AAAAGGTTGG
           1021 GTTGGGATAT TCACGCTGAA CCACGTGAAA CCTGATGGGC AGATATAAGA TTGGCCCAGG
           1081 GCAGTATAGA GTATCACCGA TCCTTTAAGT GACCATTAAG AGAATATCTA CGCCAGGCAA
          1141 AAGGTTTCCT TATGAATTGG
          //

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          original Ikeda, Y., An archaeal RNA binding protein, FAU-1, is a novel ribonuclease related to rRNA stability in Pyrococcus and Thermococcus. Sci. Rep. 7 (1): 12674 (2017). PMID 28978920.
          reference Kanai, A., Expression cloning and characterization of a novel gene that encodes the RNA-binding protein FAU-1 from Pyrococcus furiosus. Biochem. J. 372 (Pt 1): 253-261 (2003). PMID 12614195.

          2022.05.17

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