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pQE-Lrp

Expression vector of Escherichia coli K-12 MG1655 leucine-responsive regulatory protein (Lrp).

Catalog number RDB15924
Resource name pQE-Lrp
Clone info. Expression vector of Escherichia coli K-12 MG1655 leucine-responsive regulatory protein (Lrp) tagged with 6xHis at N-terminus.
Comment Expression was confirmed by the depositor with mobility shift assay.
Vector backbone Plasmid
Size of vector backbone 3.5 kb
Selectable markers Amp^r
Gene/insert name E. coli lrp genomic DNA
Depositor|Developer Yoneyama, Hiroshi |
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External Database
E. coli lrp

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Ihara, K., J. Biosci. Bioeng. 123 (4): 444-450 (2017)).
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Ihara, K., J. Biosci. Bioeng. 123 (4): 444-450 (2017))。
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          RDB15924 pQE-Lrp DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Ihara, K., Expression of the alaE gene is positively regulated by the global regulator Lrp in response to intracellular accumulation of l-alanine in Escherichia coli. J. Biosci. Bioeng. 123 (4): 444-450 (2017). PMID 28057466.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Bacteria host strains (en) (English text)
          Featured content Bacteria host strains (ja) (Japanese text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB15924_A8E8p1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pJB8-Forward
          Sequence file: RDB15924_A8E8a.seq check
          >D05325A4-5_A8E8_pJB8-Forward_A01_01_ABI08.ab1
              1 CAAAATTTAT TTGCTTTGTG AGCGGATAAC AATTTCACAC AGAATTCATT AAAGAGGAGA
             61 AATTAACTAT GAGAGGATCG CATCACCATC ACCATCACGG ATCCGTAGAT AGCAAGAAGC
            121 GCCCTGGCAA AGATCTCGAC CGTATCGATC GTAACATTCT TAATGAGTTG CAAAAGGATG
            181 GGCGTATTTC TAACGTCGAG CTTTCTAAAC GTGTGGGACT TTCCCCAACG CCGTGCCTTG
            241 AGCGTGTGCG TCGGCTGGAA AGACAAGGGT TTATTCAGGG CTATACGGCG CTGCTTAACC
            301 CCCATTATCT GGATGCATCA CTTCTGGTAT TCGTTGAGAT TACTCTGAAT CGTGGCGCAC
            361 CGGATGTGTT TGAACAATTC AATACCGCTG TACAAAAACT TGAAGAAATT CAGGAGTGTC
            421 ATTTAGTATC CGGTGATTTC GACTACCTGT TGAAAACACG CGTGCCGGAT ATGTCAGCCT
            481 ACCGTAAGTT GCTGGGGGAA ACCCTGCTGC GTCTGCCTGG CGTCAATGAC ACACGGACAT
            541 ACGTTGTTAT GGAAGAAGTC AAGCAGAGTA ATCGTCTGGT TATTAAGACG CGCTAACACG
            601 GAAGCTTAAT TAGCTGAGCT TGGACTCCTG TTGATAGATC CAGTAATGAC CTCAGAACTC
            661 CATCTGGATT TGTTCAGAAC GCTCGGTTGC CGCCGGGCGT TTTTTATTGG TGAGAATCCA
            721 AGCTAGCTTG GCGAGATTTT CAGGAGCTAA GGAAGCTAAA ATGGAGAAAA AAATCACTGG
            781 ATATACCACC GTTGATATAT CCCAATGGCA TCGTAAAGAA CATTTTGAGG CATTTCAGTC
            841 AGTTGCTCAA TGTACCTATA ACCAGACCGT TCAGCTGGAT ATTACGGCCT TTTTTAAAGA
            901 CCGTAAAGAA AAATAAGCAC AAGTTTTATC CGGGCCTTTA TTCACATTCT TGCCCGCCTG
            961 ATGAATGCTC ATCCGGGAAT TTCGTATGGC AATGAAGACG GTGAGCTGGT GATATGGGGA
           1021 TAGTTGTTCA CCCCTTTATA CACCGATTTC ATGAGCAAAC TGAAACGGTT TTCAATCGCT
          1081 CTGGGAAGTG ATTACCCAAC GGAACGGATT TATTCC
          //
          Primer: Cm_N_rev
          Sequence file: RDB15924_A8E8b.seq check
          >D05325A4-5_A8E8_Cm_N_rev_B01_02_ABI08.ab1
              1 CGGGTCCCCA AAATGGTTCT TTACGATGCC ATTGGGATAT ATCAACGGTG GTATATCCAG
             61 TGATTTTTTT CTCCATTTTA GCTTCCTTAG CTCCTGAAAA TCTCGCCAAG CTAGCTTGGA
            121 TTCTCACCAA TAAAAAACGC CCGGCGGCAA CCGAGCGTTC TGAACAAATC CAGATGGAGT
            181 TCTGAGGTCA TTACTGGATC TATCAACAGG AGTCCAAGCT CAGCTAATTA AGCTTCCGTG
            241 TTAGCGCGTC TTAATAACCA GACGATTACT CTGCTTGACT TCTTCCATAA CAACGTATGT
            301 CCGTGTGTCA TTGACGCCAG GCAGACGCAG CAGGGTTTCC CCCAGCAACT TACGGTAGGC
            361 TGACATATCC GGCACGCGTG TTTTCAACAG GTAGTCGAAA TCACCGGATA CTAAATGACA
            421 CTCCTGAATT TCTTCAAGTT TTTGTACAGC GGTATTGAAT TGTTCAAACA CATCCGGTGC
            481 GCCACGATTC AGAGTAATCT CAACGAATAC CAGAAGTGAT GCATCCAGAT AATGGGGGTT
            541 AAGCAGCGCC GTATAGCCCT GAATAAACCC TTGTCTTTCC AGCCGACGCA CACGCTCAAG
            601 GCACGGCGTT GGGGAAAGTC CCACACGTTT AGAAAGCTCG ACGTTAGAAA TACGCCCATC
            661 CTTTTGCAAC TCATTAAGAA TGTTACGATC GATACGGTCG AGATCTTTGC CAGGGCGCTT
            721 CTTGCTATCT ACGGATCCGT GATGGTGATG GTGATGCGAT CCTCTCATAG TTAATTTCTC
            781 CTCTTTAATG AATTCTGTGT GAAATTGTTA TCCGCTCACA AAGCAAATAA ATTTTTTATG
            841 ATTTCTCGAG GTGAAGACGA AAAGGGCCTC GTGATACGCC CTATTTTTTA TAGGTTAATG
            901 TCATGATAAT TAATGGGTTT CTTAGAACGT CAGGTGGCAC TTTTTCGGGG GAAATGTGCC
            961 GCGGAACCCC CTATTTGTTT ATTTTTTCTA AATACATTCC AAATATGTAT CCGCTCATGA
           1021 GACCAATAAC CCCTGATTAA ATGCCTTCCA ATAATATTGG AAAAAGGGAG AAGTATGAGG
          1081 TATTCAACAT TTTCCGTGTT CGCCCCTTTA ATTTTCCCCT GATGTTTATA GCGCG
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Ihara, K., Expression of the alaE gene is positively regulated by the global regulator Lrp in response to intracellular accumulation of l-alanine in Escherichia coli. J. Biosci. Bioeng. 123 (4): 444-450 (2017). PMID 28057466.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl