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pMRX-puro-EGFP-Rab7 (#RDB15975)

Retroviral expression vector of mouse Rab7.

Clone info. Retroviral expression vector of mouse Rab7, fused with EGFP at N-terminus.
Comment Refer AB232599 for complete cds of the mouse Rab7, complete cds,
Vector backbone pMRX-puro-EGFP (plasmid)
Selectable markers Amp^r (E. coli), Puro^r (mammalian cells).
Gene/insert name mouse Rab7 cDNA
Reference of insert sequence AB232599 (DDBJ accession number)
Depositor|Developer Fukuda, Mitsunori |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kuchitsu, Y. et al. (2018) J. Cell Sci. in press)
Additional terms and conditions:
Regarding pMRX vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literatures for pMX and pMRX vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003; Saitoh T., J. Biol. Chem. 278 (38): 36005-36012, 2003) is requested.
Remarks Remember that you will be working with samples containing infectious virus.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Kuchitsu, Y. et al. (2018) J. Cell Sci. in press)。
MTAに書く付加的使用条件：
pMRXベクターについて：利用者は、研究成果の公表にあたって pMXならびにpMRXベクターの文献 （Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003; Saitoh T., J. Biol. Chem. 278 (38): 36005-36012, 2003) の引用を必要とする。
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB15975 pMRX-puro-EGFP-Rab7 Under QC test. Please contact us. DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

How to cite this biological resource

Materials & Methods section:

The pMRX-puro-EGFP-Rab7 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15975).

Reference section:

Kuchitsu, Y., Homma, Y., Fujita, N., Fukuda, M., Rab7 knockout unveils regulated autolysosome maturation induced by glutamine starvation. J. Cell Sci. 131 (7). pii: jcs215442 (2018). PMID 29514857. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original Kuchitsu, Y., Rab7 knockout unveils regulated autolysosome maturation induced by glutamine starvation. J. Cell Sci. 131 (7). pii: jcs215442 (2018). PMID 29514857. [link to RRC of NBRP]
reference Kuroda, T.S., The Slp homology domain of synaptotagmin-like proteins 1-4 and Slac2 functions as a novel Rab27A binding domain. J. Biol. Chem., 277, 9212-9218 (2002). PMID 11773082. [link to RRC of NBRP]