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pGL4.74-HBpg-Ce900/1851

Luciferase reporter of the Hepatitis B virus (HBV) genotype Ce, pregenomic (pg) RNA promoter (nt900–nt1851).

Catalog number RDB15980
Resource name pGL4.74-HBpg-Ce900/1851
Clone info. Luciferase reporter of the Hepatitis B virus (HBV) genotype Ce, pregenomic (pg) RNA promoter (nt900–nt1851).
Comment HBV genome was artificially synthesized by Eurofins Genomics (Ebersberg, Germany), designed in accordance with the most common nucleotide observed among HBV genotype Ce clones (AB014381, AB205124, AB033551, AB198081, AY596108, AB198080, AB222714 and AY066028). HBx start codon is mutated to TAG. Expression was confirmed by the depositor with luciferase reporter assay.
Vector backbone pGL4.74 (Plasmid)
Size of vector backbone 4.2 kb
Selectable markers Amp^r
Gene/insert name HBV genomic DNA
Depositor|Developer Suzuki, Tetsuro |
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            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Sun, S et al. Sci. Rep. 7 (1) 12874, 2017)
            Additional terms and conditions for distribution The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Sun, S et al. Sci. Rep. 7 (1) 12874, 2017)。
            付加的提供条件 本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。
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            Catalog # Resource name Availability Shipping form Fee (non-profit org.)
            RDB15980 pGL4.74-HBpg-Ce900/1851 Under QC test. Please contact us. DNA solution

            check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

            References and tips

            Electronic file

            Original reference

            original Sun, S., Involvement of PUF60 in Transcriptional and Post-transcriptional Regulation of Hepatitis B Virus Pregenomic RNA Expression. Sci. Rep. 7 (1): 12874 (2017). PMID 28993636.

            Further references such as user reports and related articles (go to bottom)

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            Sequence information

            check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

            References

            Original, user report and related articles

            original Sun, S., Involvement of PUF60 in Transcriptional and Post-transcriptional Regulation of Hepatitis B Virus Pregenomic RNA Expression. Sci. Rep. 7 (1): 12874 (2017). PMID 28993636.

            2022.05.17

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