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px330-Dmd-R

Expression vector of sgRNA for right-target of mouse Dmd with hSpCas9.

Catalog number RDB16005
Resource name px330-Dmd-R
Clone info. Expression vector of sgRNA for right-target of mouse Dmd (5'TGTGTCCATATAGTACTAGG3') with hSpCas9.
Vector backbone pX330-U6-Chimeric_BB-CBh-hSpCas9 (plasmid)
Size of vector backbone 8.5 kb
Selectable markers Ampicillin
Gene/insert name mouse Dmd genomic S. pyogenes Cas9 genomic
Depositor|Developer Sugiyama, Fumihiro | Mizuno, Seiya |
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External Database
mouse Dmd
    S. pyogenes Cas9

          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Mizuno-Iijima, S. et al., Methods 191: 23-31 (2021)).
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          Remarks
          The BIOLOGICAL RESOURCE contains CRISPR/Cas9 technologies and is not provided to for-profit organization or for for-profit research by non-profit organizations.
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Mizuno-Iijima, S. et al., Methods 191: 23-31 (2021))。
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          専用MTA(CRISPR/Cas9内包遺伝子材料専用 非営利学術目的)をお使いください [Word]
          手続きの概要は、「提供申込みについて[link]」をご覧ください。
          備考
          本件リソースはCRISPR/Cas9 technologyを用いたゲノム編集バイオリソースです。営利機関および非営利機関による営利目的研究には提供いたしません。

          Catalog # Resource name Availability Shipping form Fee (non-profit org.)
          RDB16005 px330-Dmd-R Under QC test. Please contact us. DNA solution

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          How to cite this biological resource

          Materials & Methods section:

          The px330-Dmd-R was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16005).

          Reference section:

          Further references such as user reports and related articles (go to bottom)


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          QC test results

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.


          References

          Original, user report and related articles


          2023.08.17

          GNP_filter3_RDBDEP_html_230702.pl