Please review the QC test results indicated by icon below as well as clone information before placing your order.

pCX-rRobo2-Fc

Expression vector of the extracellular domain of rat slit receptor tagged with the Fc region of human IgG (Robo2-Fc).

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution

In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Sci. Rep. 7(1), 13847, 2017). RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Tsukuba University. (chizai@ilc.tsukuba.ac.jp) RECIPIENT must contact the DEPOSITOR (chizai@ilc.tsukuba.ac.jp) in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.

Ordering

Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Sci. Rep. 7(1), 13847, 2017)。 非営利機関が非営利目的の教育・研究用に用いる場合以外は、筑波大学と別途MTAを締結すること(chizai@ilc.tsukuba.ac.jp)。利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び事業活動を行う場合は、寄託者(chizai@ilc.tsukuba.ac.jp) と別途協議を行うこと。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

Reference section:

Further references such as user reports and related articles (go to bottom)

References and tips

Featured content

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB16033_A8Htp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pCAG-F_V3 (Pr0495) Region: chimeric intron, insert 5'(rRobo2 5') Sequence file: RDB16033_A8Hte.seq
>D05660B7-2_A8Ht_pCAG-F_V3_A01_01_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNT TNNNNNNNNN NNNNNNNAGG GCGGGGTTCG GCTTCTGGCG    61 TGTGANCGGC GGCTCTAGAG CCTCTGCTAA CCATGTTCAT GCCTTCTTCT TTTTCCTACA   121 GCTCCTGGGC AACGTGCTGG TTATTGTGCT GTCTCATCAT TTTGGCAAAG AATTTCGAGG   181 TCGACGGTAT CGATAAGCTT GATATCGAAT TCCTGCAGCC CAGTCTAAAC TTCGGACCTA   241 CCTCCTTTTT TGATACTTAT TTTTGTACTT CTGCTCTCTG GGATTGGTTT CTTAAACAAC   301 CTGGATCCTT TTTCATATGT CAAAATGACT CCTCTGATGT TTACACTACT ATTGCTCTTT   361 GGATTTCTCT GCATTCGGAC TGACGGATCT CGTCTTCGTC AAGAAGACTT TCCCCCCAGG   421 ATCGTAGAAC ACCCTTCTGA CGTCATCGTC TCCAAGGGAG AGCCCACCAC TCTGAACTGT   481 AAAGCAGAGG GCCGGCCCAC TCCCACCATT GAATGGTACA AGGATGGTGA GCGGGTGGAG   541 ACAGACAAGG ATGATCCCAG GTCACACAGG ATGCTTCTGC CCAGCGGATC CTTATTCTTT   601 TTGCGAATTG TTCACGGACG CAGAAGTAAA CCAGATGAAG GAACTTACGT CTGTGTTGCA   661 AGGAACTATC TTGGTGAAGC AGTGAGTCGC AATGCATCTC TGGAAGTAGC ATTATTGCGA   721 GATGACTTCC GGCAAAACCC CACAGATGTG GTAGTCGCAN CANGAGAGCC TGCANTCTTG   781 GAGTGCCAGC CACCACGGGG ACACCCNNNA CCAACCATCT ACTGNAAAAA GGACNAAGTC   841 CGAATTGATG ANAAGGANNA GAGAATAAGT ATCCGTGGTG GGAAGCTGAT GATCTCCNAT   901 ACTAGGAAAA NCGATGCTGG CNTGTATACC TGNNTGGGGA ANCNNNNNTG GNGGGNANAA 961 AGGNANNNCN ANNCTGCNNN NNTNNCNNNT CTTNNANCNA CNNNNNNNNN N //
Primer: pCAGGS_R1 (Pr0614) Region: beta-globin pA,insert 3'(hIgG Fc 3') Sequence file: RDB16033_A8Htf.seq
>16033_16033_B1JdF_pCAGGS_R1_G01_07_ABI08.ab1     1 NNNNNNNNNN NNNNNNCNNT TNNNNNNAAA ATAAATTTCC TTTATTAGCC AGAAGTCAGA    61 TGCTCAAGGG GCTTCATGAT GTCCCCATAA TTTTTGGCAG AGGGAAAAAG ATCTCAGTGG   121 TATTTGTGAG CCAGGGCATT GGCCACACCA GCCACCACCT TCTGATAGGC AGCCTGCACC   181 TGAGGAGTGA ATTTCGAGCA GCTGAAGCTT TCATTTACCC GGAGACAGGG AGAGGCTCTT   241 CTGCGTGTAG TGGTTGTGCA GAGCCTCATG CATCACGGAG CATGAGAAGA CGTTCCCCTG   301 CTGCCACCTG CTCTTGTCCA CGGTGAGCTT GCTGTAGAGG AAGAAGGAGC CGTCGGAGTC   361 CAGCACGGGA GGCGTGGTCT TGTAGTTGTT CTCCGGCTGC CCATTGCTCT CCCACTCCAC   421 GGCGATGTCG CTGGGATAGA AGCCTTTGAC CAGGCAGGTC AGGCTGACCT GGTTCTTGGT   481 CAGCTCATCC CGGGATGGGG GCAGGGTGTA CACCTGTGGT TCTCGGGGCT GCCCTTTGGC   541 TTTGGAGANG GTTTTCTCGA TGGGGGCTGG GANGGCTTTG TTGGAGACCT TGCACTTGTA   601 CTCCTTGNCA TTCNGCCNGT CCTGGNGCAN GANGGTGANG ANGCTGANNN CNCNGTACNT   661 GCTGNTGNAC NGNTNCTCCC GCGGCTTTGN CNNNGNNNNN TGNNNNTNCN NNNNNNCNNN 721 NNANNNNNNN NNN //
Primer: rRobo2-M_F (Pr1069) Region: insert mid(rRobo2 3',hIgG Fc 5') Sequence file: RDB16033_A8Htg.seq
>16033_16033_B1JdF_rRobo2-M_F_B01_02_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNGG NGGNNCGACN ACCCCNGTTT ATTCNGGGCT ACAGAGTGAT    61 GTACCGACAG ACTTCTGGTC TACAAGCTTC AACTGTGTGG CAGAATCTAG ATGCCAAAGT   121 CCCGACTGAG AGGAGTGCTG TCCTTGTGAA TCTGAAAAAG GGGGTGACTT ATGAAATTAA   181 AGTCAGGCCA TATTTCAATG AGTTCCAAGG AATGGACAGT GAATCAAAAA CAATCCGTAC   241 TACTGAGGAA GCTCCAAGTG CGCCTCCCCA GTCTGTCACG GTGCTGACCG TTGGAAGCCA   301 TAACAGCACA AGCATTAGTG TTTCCTGGGA TCCTCCACCA GCCGACCACC AGAATGGAAT   361 CATACAGGAA TACAGATCTG GTCGACTGAT GCTTGGTCCC GGGCTCGACA AAACTCACAC   421 ATGCCCACCG TGCCCAGCAC CTGAACTCCT GGGGGGACCG TCAGTCTTCC TCTTCCCCCC   481 AAAACCCAAG GACACCCTCA TGATCTCCCG GACCCCTGAG GTCACATGCG TGGTGGTGGA   541 CGTGAGCCAC GAAGANCCTG AGGTCAAGTT CAACTGGTAC GTGGACGGCG TGGANGTGCA   601 TAATGNCNAG ACAAAGCCGC GGGANGAGCA NTACNACAGC ACGTACCGTG TGGTCNGCGT   661 CCTCNCCGTC CTGCANNANG NNNGGNTGNA TGNNNNNNAN TANNNNNGNN NNNNCNNNNN 721 NNNCNNNNNN NCCNNNNNNN NAANNNNNNT NNNNNNNNNN //
Primer: SV40pro_ori_F (Pr0731) Region: SV40 pro_ori,SV40 pA,ori Sequence file: RDB16033_A8Hth.seq
>16033_16033_B1JdF_SV40pro_ori_F_H01_08_ABI08.ab1     1 NNNNNNNNNN NNNNNGCCGC CTCNGCCTCT GANCTATTCC NGAAGTAGTG AGGAGGCTTT    61 TTTGGAGGCC TAGGCTTTTG CAAAAAGCTA ACTTGTTTAT TGCAGCTTAT AATGGTTACA   121 AATAAAGCAA TAGCATCACA AATTTCACAA ATAAAGCATT TTTTTCACTG CATTCTAGTT   181 GTGGTTTGTC CAAACTCATC AATGTATCTT ATCATGTCTG GATCCGCTGC ATTAATGAAT   241 CGGCCAACGC GCGGGGAGAG GCGGTTTGCG TATTGGGCGC TCTTCCGCTT CCTCGCTCAC   301 TGACTCGCTG CGCTCGGTCG TTCGGCTGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT   361 AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA   421 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC   481 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT   541 ATAAAGATAC CAGGCGTTTC CCCCTGGAAN CTCCCTCGTG CGCTCTCCTN NTNNCGACCC   601 NGCCGCTTAC CGGANACCTG TCCGCCNTTC NCCCTTCNGG AAGCGTNNNG CTTTCTNNTA   661 NCTNACGCTG NNNNATCTCA NTTCGGNGNN NNCGNNNNNT NNNNNNNGGG NNGNGNGNNN 721 NNNNNNCCNN NNNNNNCNNA NNNNNNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.05.01

GNP_filter3_RDBDEP_html_230501.pl