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pT2/CMV-PDGFA-IRES-DsRed

The Sleeping Beauty (SB) transposon system clone. Expression vector of human PDGFA.

Catalog number RDB16061
Resource name pT2/CMV-PDGFA-IRES-DsRed
Alternative name P vector
Clone info. The Sleeping Beauty (SB) transposon system clone. Expression vector of human platelet-derived growth factor subunit A (PDGFA). DsRed is expressed as a marker. Please refer to Fig.1A of Sumiyoshi, K. et al. Cancer Sci., 109 (5): 1513-1523, 2018.
Comment Expression was confirmed by the depositor with immunohistochemistry.
Vector backbone pT2/Onc2 (plasmid)
Size of vector backbone 5.1 kb
Selectable markers Amp^r
Gene/insert name Human PDGFA cDNA
Depositor|Developer Koso, Hideto |
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External Database
Human PDGFA

          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Sumiyoshi, K. et al. Cancer Sci., 109 (5): 1513-1523, 2018).
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          Remarks This clone is provided directly from Dr. Hideto Koso of the Institute of Medical Science, Tokyo University.
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Sumiyoshi, K. et al. Cancer Sci., 109 (5): 1513-1523, 2018)。
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          本件遺伝子材料の依頼に際し、専用MTA(英語版のみ)の提出をお願いします。当室にご請求ください。
          手続きの概要は、「提供申込みについて[link]」をご覧ください。
          備考 本クローンは、東京大学医科学研究所の高祖秀登先生から直接提供いたします。

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          RDB16061 pT2/CMV-PDGFA-IRES-DsRed DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The pT2/CMV-PDGFA-IRES-DsRed was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16061).

          Reference section:

          Sumiyoshi, K., Koso, H., Watanabe, S., Spontaneous development of intratumoral heterogeneity in a transposon-induced mouse model of glioma. Cancer Sci. 109 (5): 1513-1523 (2018). PMID 29575648. [link to RRC of NBRP]

          Further references such as user reports and related articles (go to bottom)


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          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB16061_A8Idp1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV-Forward
          Sequence file: RDB16061_A8Ida.seq check
          >D05854A2-3_A8Id_CMV-Forward_A01_01_ABI24.ab1
              1 CTTTACCACT TCCGCCCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC
             61 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCGCT AGCGCTACCG GACTCAGATC
            121 TCGAGCTCAA GCTTCGAATT CACTAGTGAT TGCGATGAGG ACCTTGGCTT GCCTGCTGCT
            181 CCTCGGCTGC GGATACCTCG CCCATGTTCT GGCCGAGGAA GCCGAGATCC CCCGCGAGGT
            241 GATCGAGAGG CTGGCCCGCA GTCAGATCCA CAGCATCCGG GACCTCCAGC GACTCCTGGA
            301 GATAGACTCC GTAGGGAGTG AGGATTCTTT GGACACCAGC CTGAGAGCTC ACGGGGTCCA
            361 CGCCACTAAG CATGTGCCCG AGAAGCGGCC CCTGCCCATT CGGAGGAAGA GAAGCATCGA
            421 GGAAGCTGTC CCCGCTGTCT GCAAGACCAG GACGGTCATT TACGAGATTC CTCGGAGTCA
            481 GGTCGACCCC ACGTCCGCCA ACTTCCTGAT CTGGCCCCCG TGCGTGGAGG TGAAACGCTG
            541 CACCGGCTGC TGCAACACGA GCAGTGTCAA GTGCCAGCCC TCCCGCGTCC ACCACCGCAG
            601 CGTCAAGGTG GCCAAGGTGG AATACGTCAG GAAGAAGCCA AAATTAAAAG AAGTCCAGGT
            661 GAGGTTAGAG GAGCATTTGG AGTGCGCCTG CGCGACCACA AGCCTGAATC CGGATTATCG
            721 GGAAGAGGAC ACGGGAAGGC CTAGGGAGTC AGGTAAAAAA CGGAAAAGAA AAAGGTTAAA
            781 ACCCACCTAA AGCAGCCAAC CAGATAATCG AATTCTGCAG TCGACGGTAC CGCGGGCCCG
            841 GGATCCGCCC CTCTCCCTCC CCCCCCCCCC CTAACGTTAC TGGGCCGAAG CCCGCTTGGA
            901 AATAAAGGCC GGTGTGCGTT TGTCTATATG TTATTTTCCA CCATATTGCC GTCTTTTTGG
            961 CAATGTGAGG GCCCGGGAAC CTTGACCCTG TCTTCTTGAA CGAGCATCTT AGGGTCCTTT
          1021 CCCCTCTCGC AAGGATGCAG GTCTGATTGT ATGTCCGTGA AGGAAGCAAG
          //
          Primer: Red-N_R
          Sequence file: RDB16061_A8Idb.seq check
          >D05854A2-3_A8Id_Red-N_R_B01_04_ABI24.ab1
              1 GTTATAATCG GCAACTTGAA GCGCATGAAC TCCTTGATGA CGTCCCTCGG AGGAGGCCAT
             61 GGTTGTGGCC ATATTATCAT CGTGTTTTTC AAAGGAAAAC CACGTCCCCG TGGTTCGGGG
            121 GGCCTAGACG TTTTTTTAAC CTCGACTAAA CACATGTAAA GCATGTGTAC CGAGGCCCCA
            181 GATCAGATCC CATACAATGG GGTACCTTCT GGGCATCCTT CAGCCCCTTG TTGAATACGC
            241 TTGAGGAGAG CCATTTGACT CTTTCCACAA CTATCCAACT CACAACGTGG CACTGGGGTT
            301 GTGCCGCCTT TGCAGGTGTA TCTTATACAC GTGGCTTTTG GCCGCAGAGG CACCTGTCGC
            361 CAGGTGGGGG GTTCCGCTGC CTGCAAAGGG TCGCTACAGA CGTTGTTTGT CTTCAAGAAG
            421 CTTCCAGAGG AACTGCTTCC TTCACGACAT TCAACAGACC TTGCATTCCT TTGGCGAGAG
            481 GGGAAAGACC CCTAGGAATG CTCGTCAAGA AGACAGGGCC AGGTTTCCGG GCCCTCACAT
            541 TGCCAAAAGA CGGCAATATG GTGGAAAATA ACATATAGAC AAACGCACAC CGGCCTTATT
            601 CCAAGCGGCT TCGGCCAGTA ACGTTAGGGG GGGGGGAGGG AGAGGGGCGG ATCCCGGGCC
            661 CGCGGTACCG TCGACTGCAG AATTCGATTA TCTGGTTGGC TGCTTTAGGT GGGTTTTAAC
            721 CTTTTTCTTT TCCGTTTTTT ACCTGACTCC CTAGGCCTTC CCGTGTCCTC TTCCCGATAA
            781 TCCGGATTCA GGCTTGTGGT CGCGCAGGCG CACTCCAAAT GCTCCTCTAA CCTCACCTGG
            841 ACTTCTTTTA ATTTTGGCTT CTTCCTGACG TATTCCACCT TGGCCACCTT GACGCTGCGG
            901 TGGTGGACGC GGGAGGGCTG GCACTTGACA CTGCTCGTGT TGCAGCAGCC GGGTGCAGCG
            961 TTTCCACCCT CCACGCACGG GGGCCCAGAT CAGGGAAAGT TGGGCGGACG TGGGGTCGAC
           1021 CTTGAACTTC CGAAGATCTC GTAAATGGAC GTCCCTGGTC TTGCAGAACA GCCGGGACAG
          1081 GCTACTCGAT GCATTTCATC CGTA
          //
          Primer: M13_-40
          Sequence file: RDB16061_A8Idc.seq check
          >D05854A2-3_A8Id_M13_-40_C01_07_ABI24.ab1
              1 CACAGTGACG CGCGTATACG ACTCACTATA GGGCGAATTG GAGCTCGGAT CCCTATACAG
             61 TTGAAGTCGG AAGTTTACAT ACACTTAAGT TGGAGTCATT AAAACTCGTT TTTCAACTAC
            121 TCCACAAATT TCTTGTTAAC AAACAATAGT TTTGGCAAGT CAGTTAGGAC ATCTACTTTG
            181 TGCATGACAC AAGTCATTTT TCCAACAATT GTTTACAGAC AGATTATTTC ACTTATAATT
            241 CACTGTATCA CAATTCCAGT GGGTCAGAAG TTTACATACA CTAAGTTGAC TGTGCCTTTA
            301 AACAGCTTGG AAAATTCCAG AAAATGATGT CATGGCTTTA GAAGCTATCT AGTTATTAAT
            361 AGTAATCAAT TACGGGGTCA TTAGTTCATA GCCCATATAT GGAGTTCCGC GTTACATAAC
            421 TTACGGTAAA TGGCCCGCCT GGCTGACCGC CCAACGACCC CCGCCCATTG ACGTCAATAA
            481 TGACGTATGT TCCCATAGTA ACGCCAATAG GGACTTTCCA TTGACGTCAA TGGGTGGAGT
            541 ATTTACGGTA AACTGCCCAC TTGGCAGTAC ATCAAGTGTA TCATATGCCA AGTACGCCCC
            601 CTATTGACGT CAATGACGGT AAATGGCCCG CCTGGCATTA TGCCCAGTAC ATGACCTTAT
            661 GGGACTTTCC TACTTGGCAG TACATCTACG TATTAGTCAT CGCTATTACC ATGGTGATGC
            721 GGTTTTGGCA GTACATCAAT GGGCGTGGAT AGCGGTTTGA CTCACGGGGA TTTCCAAGTC
            781 TCCACCCCAT TGACGTCAAT GGGAGTTTGT TTTGGCACCA AAATCAACGG GACTTTCCAA
            841 AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG GTAGGCGTGT ACGGTGGGAG
            901 GTCTATATAA GCAGAGCTGG TTTAGTGAAA CCGTCAGATC CGCTAGCGCT ACCGGGACTC
            961 AGATCTCGAG CTCAAGCTTC GAAATTTCAC TAGTGATTGC GATGAAGGAC CTTTGGGCTT
           1021 TGCCCTGCTG CTCGTCGACT GGCCGGATAC GATCCGCCCA TGTCTGCGAA GGAAGCCGAG
          1081 ATCCCCCCGC CGAG
          //
          Primer: reverse2
          Sequence file: RDB16061_A8Idd.seq check
          >D05854A2-3_A8Id_reverse2_D01_10_ABI24.ab1
              1 AGTAAGGCAT TCGCCAAGCG CGCATTAACC CTCACTAAAG GGAACAAAAG CTGGGTACCG
             61 GGCCCCCCCT CGAGGTCGAC TCTAGCTAGA GGATCCCTAT ACAGTTGAAG TCGGAAGTTT
            121 ACATACACCT TAGCCAAATA CATTTAAACT CACTTTTTCA CAATTCCTGA CATTTAATCC
            181 TAGTAAAAAT TCCCTGTCTT AGGTCAGTTA GGATCACCAC TTTATTTTAA GAATGTGAAA
            241 TATCAGAATA ATAGTAGAGA GAATGATTCA TTTCAGCTTT TATTTCTTTC ATCACATTCC
            301 CAGTGGGTCA GAAGTTTACA TACACTCAAT TAGTATTTGG TAGCATTGCC TTTAAATTGT
            361 TTAACTTGGG TCAAACATTT CGAGTAGCCT TCCACAAGCT AGATCCCCTA GTTTGTGATA
            421 GGAATTAGCT TTTCCGCAAG CTCTAGTCGA GCCCCAGCTG GTTCTTTCCG CCTCAGAAGC
            481 CATAGAGCCC ACCGCATCCC CAGCATGCCT GCTATTGTCT TCCCAATCCT CCCCCTTGCT
            541 GTCCTGCCCC ACCCCACCCC CCAGAATAGA ATGACACCTA CTCAGACAAT GCGATGCAAT
            601 TTCCTCATTT TATTAGGAAA GGACAGTGGG AGTGGCACCT TCCAGGGTCA AGGAAGGCAC
            661 GGGGGAGGGG CAAACAACAG ATGGCTGGCA ACTAGAAGGC ACAGTCGAGG CTGATCAGCG
            721 AGCTCTAGAG AATTGATCGC GGCCGCTACA GGAACAGGTG GTGGCGGCCC TCGGCGCGCT
            781 CGTACTGCTC CACGATGGTG TAGTCCTCGT TGTGGGAGGT GATGTCCAGC TTGGAGTCCA
            841 CGTAGTAGTA GCCGGGCAGC TGCACGGGCT TCTTGGCCAT ATAGATAGAC TTGAACTCCA
            901 CCAGGTAGTG GCCGCCGTCC TTCAGCTTCA GGGCCCTTGT GGATCTCGCC CTTCAGCACG
            961 CCGTCGCGGG GGGTACAGCG CTCGGGTGGG AGGCCCTCCC AGCCCATAGT TCTTCGTCTG
           1021 CATTTACGGG GCCGGTCGAA GGGGAGGTTC ACGTCGATGG AACCTCACCT TGCTAGGATG
          1081 GAAGGGAGC
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Sumiyoshi, K., Spontaneous development of intratumoral heterogeneity in a transposon-induced mouse model of glioma. Cancer Sci. 109 (5): 1513-1523 (2018). PMID 29575648. [link to RRC of NBRP]

          2023.12.13

          GNP_filter3_RDBDEP_html_231213.pl