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pcDNA3-FLAG-mouse p50

Expression vector of mouse nuclear factor of kappa light polypeptide gene enhancer in B cells 1, p105 (Nfkb1) tagged with FLAG.

Catalog number RDB16743
Resource name pcDNA3-FLAG-mouse p50
Clone info. Expression vector of mouse nuclear factor of kappa light polypeptide gene enhancer in B cells 1, p105 (Nfkb1) tagged with FLAG.
Comment This clone contains a cDNA fragment corresponding to the 503 nt to 1600 nt of NM_008689.2. Known difference (at least) : A to G substitution at 1405nt (Thr, synonym).
Vector backbone pcDNA3 (plasmid)
Selectable markers Amp^r
Gene/insert name mouse Nfkb1 cDNA
Depositor|Developer Maruyama, Takashi |
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External Database
mouse Nfkb1

          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (J. Biol. Chem. 276 (29): 27657-27662, 2001) and an acknowledgment to the DEPOSITOR and Dr. Tatsushi Muta are requested.
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(J. Biol. Chem. 276 (29): 27657-27662, 2001)。利用者は、研究成果の公表にあたって寄託者ならびに牟田達史博士への謝辞の表明を必要とする。
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          RDB16743 pcDNA3-FLAG-mouse p50 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The pcDNA3-FLAG-mouse p50 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16743).

          Reference section:

          Yamazaki, S., Muta, T., Takeshige, K., A novel IkappaB protein, IkappaB-zeta, induced by proinflammatory stimuli, negatively regulates nuclear factor-kappaB in the nuclei. J. Biol. Chem. 276 (29): 27657-27662 (2001). PMID 11356851.

          Further references such as user reports and related articles (go to bottom)


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          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB16743_A8Ipp1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV_Forward (Pr0016)
          Region: CMV pro,T7 pro,FLAG,insert 5'
          Sequence file: RDB16743_A8Ipa.seq check
          >D05839C8-A8Ip_1_CMV_Foward_D01_10_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNTG NNNNNNNGGG CGGTAGGCGT GTACGGTGGG AGGTCTATAT
             61 AAGCAGAGCT CTCTGGCTAA CTAGAGAACC CACTGCTTAC TGGCTTATCG AAATTAATAC
            121 GACTCACTAT AGGGAGACCC AAGCTGCCAC CATGGACTAC AAGGACGACG ATGACAAGAA
            181 GCTTGGTACC GAGCTCGGAT CCATGGCAGA CGATGATCCC TACGGAACTG GGCAAATGTT
            241 TCATTTGAAC ACTGCTTTGA CTCACTCAAT ATTTAATGCA GAATTATATT CACCAGAAAT
            301 ACCACTGTCA ACAGATGGCC CATACCTTCA AATATTAGAG CAACCAAAAC AGAGGGGATT
            361 TCGATTCCGC TATGTGTGTG AAGGCCCATC ACACGGAGGG CTTCCGGGAG CCTCTAGTGA
            421 GAAGAACAAG AAATCCTACC CACAGGTCAA AATTTGCAAC TATGTGGGGC CTGCAAAGGT
            481 TATCGTTCAG TTGGTCACAA ATGGAAAAAA CATCCACCTG CACGCCCACA GCCTGGTGGG
            541 CAAGCACTGT GAGGACGGGG TATGCACCGT AACAGCAGGA CCCAAGGACA TGGTGGTTGG
            601 CTTTGCAAAC CTGGGAATAC TTCATGTGAC TAAGAAAAAG GTATTTGAAA CACTGGAAGC
            661 ACGGATGACA GAGGCGTGTA TTAGGGGCTA TAATCCTGGA CTTCTGGTGC ATTCTGACCT
            721 TGCCTATCTA CAAGCAGAAN GCGGANGAGA NCGGCAACTC ACAGACAGAG AGAANGAGAT
            781 CATCCGCCAG GCAGCCGTGC AGCAGACCAA GGAGATGGAC CTGANCGTGG TGCGCCTCAT
            841 GTTCACAGCC TTCCTCCCTG ACAGCACTGG CAGCTTCANT CGGANNNTGG ANCCTGTGGT
            901 GNCAGANNNC ATCTATNATA GCAAAGCCCN NAATGNATNC NACCNGAAAA TCGTGNNAAT
          961 GGNNANNNNA GCNNNTGTNN GNNNGNNNGG NNNNNNNNNT NNNNNNNNNN
          //
          Primer: BGH_rev2 (Pr0606)
          Region: bGH pA,SP6 pro,insert 3'
          Sequence file: RDB16743_A8Ipb.seq check
          >D05839C8-A8Ip_1_BGH_rev2_E01_13_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNGG GGGNNGGGCN AACAACAGAT GGCTGGCAAC TAGAAGGCAC
             61 AGTCGAGGCT GATCAGCGAG CTCTAGCATT TAGGTGACAC TATAGAATAG GGCCCTCTAG
            121 ATGCATGCTC GAGCGGCCGC CAGTGTGATG GATATCTGCA GAATTCCTAC GGCATAAGCT
            181 TCTGGCGTTT CCTTTGCACT TCCTCTTTGT CTTTGATTTC AGGGTAGTAG AGAAAGGGTT
            241 TCGGTTCACT AGTTTCCAGG TCTGATTTCC TCCGAAGCTG AACAAACACG GAAGCTGGCT
            301 TTGTAATGTT GACATCCTTA TACTTTGGCG TTTTGAAGAC AATGGCAAAC TGTCTATGAA
            361 CATCCGTGGG GGAAAAGTCC CCAAATCCTT CCCAAACTCC GCCATTTTCT TCCTCTTCAT
            421 AAAACCGAAT CTGGATGTCA TCTTTCTGAA CCTTGTCACA GAGAAGGTAA ATCTCCTCCC
            481 CTCCCGTCAC ACATCCTGCT GTTCTGTCCA TTCTCACGAT TTTCAGGTTG GATGCATTCG
            541 GGGCTTTGCT ATCATAGATG GCGTCTGACA CCACAGGCTC CAGTCTCCGA GTGAAGCTGC
            601 CAGTGCTGTC AGGGAGGAAG GCTGTGAACA TGAGGCGCAC CACGCTCAGG TCCATCTCCT
            661 TGGTCTGCTG CACGGCTGCC TGGCGGATGA TCTCCTTCTC TCTGTCTGTG AGTTGCCGGT
            721 CTCCTCCGCC TTCTGCTTGT AGATAGGCAA GGTCAGAATG CACCAGAAGT CCAGGANTAT
            781 AGCCCCTAAT ACACGCCTCT GTCATCCGTG CTTCCAGTGT TTCAAATACC TTTTTCTTAN
            841 NCACATGAAG TATTCCCAGG TTTGCAAAGC CNACCACCAT GTCCTTNNGT NCNGCTGTTA
            901 CGGNGCATAC CCCNNCCNCA CAGNGCTNGC CNACNNNNCN NNNGGCNNGC NNGNNNANGN
          961 TTTTTNNNTT TNNGANNNNN NNAANNNNNN NNNTNNNNNN NCNNNNNNNN NN
          //
          Primer: SV40pro_F (Pr0110)
          Region: NeoR
          Sequence file: RDB16743_A8Ipc.seq check
          >D05839C8-A8Ip_1_SV40pro_F_F01_16_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNTG NNGNNGNTTT TTTGGAGGCC TAGGCTTTTG
             61 NNNNNAGCTC CCGGGAGCTT GTATATCCAT TTTCGGATCT GATCAAGAGA CAGGATGAGG
            121 ATCGTTTCGC ATGATTGAAC AAGATGGATT GCACGCAGGT TCTCCGGCCG CTTGGGTGGA
            181 GAGGCTATTC GGCTATGACT GGGCACAACA GACAATCGGC TGCTCTGATG CCGCCGTGTT
            241 CCGGCTGTCA GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG ACCGACCTGT CCGGTGCCCT
            301 GAATGAACTG CAGGACGAGG CAGCGCGGCT ATCGTGGCTG GCCACGACGG GCGTTCCTTG
            361 CGCAGCTGTG CTCGACGTTG TCACTGAAGC GGGAAGGGAC TGGCTGCTAT TGGGCGAAGT
            421 GCCGGGGCAG GATCTCCTGT CATCTCACCT TGCTCCTGCC GAGAAAGTAT CCATCATGGC
            481 TGATGCAATG CGGCGGCTGC ATACGCTTGA TCCGGCTACC TGCCCATTCG ACCACCAAGC
            541 GAAACATCGC ATCGAGCGAG CACGTACTCG GATGGAAGCC GGTCTTGTCG ATCAGGATGA
            601 TCTGGACGAA GAGCATCAGG GGCTCGCGCC AGCCGAACTG TTCGCCAGGC TCAAGGCGCG
            661 CATGCCCGAC GGCGAGGNTC TCGTCGTGAC CCATGGCGAT GCCTGCTTGC CGAATATCAT
            721 GGTGGAAAAT GGCCGCTTTT CTGGATTCAT CGACTGTGGC CGGCTGGGTG TGGCGGACCG
            781 CTATCANGAC ATANCGTTGG CTACCCGTGA TATTGCTGAA NAGCTTGGCG GCGAATGGGC
            841 TGACCGCTTC CTCGTGNTTT ACNGNATCGC CGCNNCCNAT TCGCANCGCA TCGCCTTCTA
            901 TCGCCNTNCT TGACNANTNN TTCTGANCNG GGACTCTGGG GGNNNGAAAT GACCNNANNN
            961 ANGCGANNCC CNAANCNNGC NNTNNNNNAG ANTTTNNANT TNCNNNCNNN NNGNCNNTNC
          1021 NANNNNNNNN NTNNNNNNNN NN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Yamazaki, S., A novel IkappaB protein, IkappaB-zeta, induced by proinflammatory stimuli, negatively regulates nuclear factor-kappaB in the nuclei. J. Biol. Chem. 276 (29): 27657-27662 (2001). PMID 11356851.

          2023.05.01

          GNP_filter3_RDBDEP_html_230501.pl