Please review the QC test results indicated by icon below as well as clone information before placing your order.

pCAGGS-ChRGR-Venus

Expression vector of organelle optogenetics channelrhodopsin.

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In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Asano, T., Front. Neurosci. 12: 561, 2018). For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.

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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Asano, T., Front. Neurosci. 12: 561, 2018)。営利機関の利用希望者は、事前に利用条件等につき寄託者と合意し、提供承諾を得ること。
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Please review the QC test results indicated by icon below as well as clone information before placing your order.

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB16748_A8Jnp1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pCAG-F (Pr0328) Region: intron, insert 5' Sequence file: RDB16748_A8Jne.seq
>16748_16748_B0A9S_pCAG-F_F04_06_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN GGCGGGGTTC GGCTTCTGGC    61 GTGTGANCGG CGGCTCTANA NNCNCTGCTA ACCATGTTCA TGCCTTCTTC TTTTTCCTAC   121 AGCTCCTGGG CAACGTGCTG GTTATTGTGC TGTCTCATCA TTTTGGCAAA GAATTCGCCA   181 CCATGGCTCG GAGACCTTGG CTGCTGGCAC TGGCTCTGGC TGTCGCTCTG GCCGCTGGCT   241 CTGCTGGGGC TTCTACTGGA TCTGACGCAA CAGTGCCCGT CGCTACTCAG GACGGACCTG   301 ATTACGTGTT CCACAGGGCA CATGAGCGCA TGCTGTTTCA GACATCTTAT ACTCTGGAAA   361 ACAATGGCAG TGTCATCTGC ATTCCCAACA ATGGGCAGTG CTTCTGTCTG GCATGGCTGA   421 AGTCTAACGG CACCAATGCC GAGAAACTGG CCGCTAACAT CCTGCAGTGG ATTACATTCG   481 CCCTGTCCGC TCTGTGCCTG ATGTTTTACG GATATCAGAC CTGGAAGTCT ACATGTGGCT   541 GGGAGGAAAT CTACGTGGCT ACCATCGAGA TGATCAAGTT CATCATTGAA TACTTCCACG   601 AGTTTGACGA ACCTGNCNNG ATCTATAGCT CCAACGGGAA TAAGACTGTC TGGCTGANAT   661 NCNCTGAGTG GCTGCTGACC TGTCCAGTGA TCCTGATTCA TCTGAGCAAC CTGACTGGNC   721 TGGCCAACGA CTATAATAAG AGAANCCATG GGGGCTGCTG GTGANTGATA TCGGAACAAT   781 TGTCTGGGNN NNNNCANCAN NNNTNNCNAA AGGCTATGTG CGCGTCATCT TCTTTCTGAT   841 NGGNNCTGTG CTACNGGATC TNNACATTCT TTAANGNNNN CNNAANGNGT NCNTCGAGGC   901 NTNNNCNCNC AGTNCCTANN NNGNNNGGTG TANACNGGNN GNNCNCNNGN ANNGGCNTNN   961 NTGNTNNTTT GNGTNCNNGG GGNANGTNNC NNNTNCNGNT TNNTGNNGGN NNCCNNANGN 1021 ATNCNGGNNN NNTTNNNNCN NNNTNNNNTN NNNNNNNNN //
Primer: Venus-N_R (Pr0341) Region: Venus 5', insert 3' Sequence file: RDB16748_A8Jnf.seq
>D05919A3_A8Jn_1_Venus-N_R_B02_02_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NTTGTGNNCG TTTNNGTCGC CGNNCNNGCT CGNNCAGNNN    61 GGGCACCACC CCGGTGAACA GCTCCTCGCC CTTGCTCACC ATGGTGGCGA CCGGTGGATC   121 CTTCCCAGTC CCTTTGTTCA CGGCTCCTGC CTCGGCTTCA TCTTCCACCA GTGTTTCCAC   181 CTCAATTTCG GTCCCGCCGA TATTCAGTTT GGTAGTCTTC CGAATATCGC CATGAATCAG   241 GATATGCTCG TGGATCAGCA CTCGCAGGTA GTGTCCCAGC AGCCCCCAGC AGTTTTTGCT   301 GGCCAGGTCC AGAATTGCAT GAGCGATGGC TGAATTAAAC TGGTTAATGT GGCCGAATCC   361 TTCGGGTCCC AGCAGAAACA GGATTGGGAA CATCCCCCAG GACACAAAGA ACAGCCAGGC   421 CATTCCAGTG ACCACCTGTC TACACCGCCC TTTAGGGACT GTGTGATATG CCTCGATGTA   481 CACCTTTGCA GCATTAAAGA ATGTGTAGAT CCCGTAGCAC AGGCCCATCA GAAAGAAGAT   541 GACGCGCACA TAGCCTTTTG ACAGGGCTGC TGTGGTGCCC CAGACAATTG TTCCGATATC   601 ACTCACCAGC AGCCCCATGG TTCTCTTATT ATAGTCGTTG GCCAGGCCAG TCAGGTTGCT   661 CAGATGAATC AGGATCACTG GACAGGTCAG CAGCCACTCA GCGTATCTCA GCCAGACAGT   721 CTTATTCCCG TTGGAGCTAT AGATCACGGC AGGTTCGTCA AACTCGTGGA AGTATTCAAT   781 GATGAACTTG ATCATCTCGA TGGTAGCCAC GTANATTTCN TCCCAGCCAC ATGTANACTT   841 CCAGGNCTGA NATCCGTAAA ACATCANGNA CAGANCGGNN NNGGCGAATG NNNTNNNNNG 901 CNNGNANGTT ANCNNCCANT TTNNNGNNNN NGGNNCCNNT NNGANTNNNN NNNNNN //
Primer: pCAGGS_R1 (Pr0614) Region: b-globin p(A), Venus 3' Sequence file: RDB16748_A8Jng.seq
>16748_16748_B0A9S_pCAGGS_R1_G04_07_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNTTGNNATG ANNNNAAATT TCCTTTATTA GCCAGAAGTC    61 AGATGCTCAA GGGGCTTCAT GATGTCCCCA TAATTTTTGG CAGAGGGAAA AAGATCTCAG   121 TGGTATTTGT GAGCCAGGGC ATTGGCCACA CCAGCCACCA CCTTCTGATA GGCAGCCTGC   181 ACCTGAGGAG TGAATTCTCA TTACTTGTAC AGCTCGTCCA TGCCGAGAGT GATCCCGGCG   241 GCGGTCACGA ACTCCAGCAG GACCATGTGA TCGCGCTTCT CGTTGGGGTC TTTGCTCAGG   301 GCGGACTGGT AGCTCAGGTA GTGGTTGTCG GGCAGCAGCA CGGGGCCGTC GCCGATGGGG   361 GTGTTCTGCT GGTAGTGGTC GGCGAGCTGC ACGCCGCCGT CCTCGATGTT GTGGCGGATC   421 TTGAAGTTGG CCTTGATGCC GTTCTTCTGC TTGTCGGCGG TGATATAGAC GTTGTGGCTG   481 TTGTAGTTGT ACTCCAGCTT GTGCCCCAGG ATGTTGCCGT CCTCCTTGAA GTCGATGCCC   541 TTCAGCTCGA TGCGGTTCAC CAGGGTGTCG CCCTCGAACT TCACCTCGGC GCGGGTCTTG   601 TAGTTGCCGT CGTCCTTGAA GAAGANGGTG CGCTCCTGGA CGTAGCCTTC GGGCATGGCG   661 GACTTGAAGA AGTCGTGCTG CTTCATGTGG TCGGGGTAGC GGGCGAAGCA CTGCAGGCCG   721 TAGCCCAGGG TGGTCACGAN GGTGGGCCAG GGCACGGGCA GCTTGCCGGT GGTGCAGATC   781 AGCTTCAGGG TCAGCTTGCC GTANGTGGNA TCGCCCTCGC CCTCGCCGGA NACGCTGAAN   841 TTGTGGNCGT TTACGTCGCC GTCCAGCTCG ANCANGANGG GCACCACCCC GGTGAANAGC   901 TCCNCGCCCT TGNTCNNCAN GGNNNNNANC GGNNGNNNCT TNCNNGNCCC TTTGNTCNNN   961 NNTNCNGNCN CNGCTTCNTN NNNNNNCANN GNTTCNNNCN NNNNTTCGGN CCNNNNNNNN 1021 NN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.11.23

GNP_filter3_RDBDEP_html_231027.pl