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Yeast NADPH-P450 reductase/pBKS- (#RDB16760)

Cloning vector of S. cerevisiae NADPH-P450 reductase.

Clone info. Cloning vector of S. cerevisiae NADPH-P450 reductase.
Comment Known differences (at least): substitutions G to A (Pro, synonym) at 641nt, A to C (Asn to Thr) at 1903nt and G to T (Gly to Val) at 2056nt of CP036477.1.
Vector backbone pBluescript KS- (plasmid)
Size of vector backbone 3.0 kb
Selectable markers Amp^r
Gene/insert name Saccharomyces cerevisiae NADPH-P450 reductase cDNA
Depositor|Developer Aoyama, Yuri |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Kitahama, Y et al. Biol. Pharm. Bull. 32(4), 558-63, 2009) is requested.
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提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 （Kitahama, Y et al. Biol. Pharm. Bull. 32(4), 558-63, 2009）の引用を必要とする。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB16760 Yeast NADPH-P450 reductase/pBKS- DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The Yeast NADPH-P450 reductase/pBKS- was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16760).

Reference section:

Kitahama, Y., Nakamura, M., Yoshida, Y., Aoyama, Y., The construction and characterization of self-sufficient lanosterol 14-demethylase fusion proteins consisting of yeast CYP51 and its reductase. Biol. Pharm. Bull. 32 (4): 558-563 (2009). PMID 19336884. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB16760_A8K1p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40 ()
Region: insert 5'
Sequence file: RDB16760_A8K1a.seq check
>D05837A8_A8K1_2_M13_-40_D03_12_ABI24.ab1
    1 AAGCCAATTG GCGCGCGGTA ATACGACTCA CTATAGGGCG AATTGGAGCT CCACCGCGGT
   61 GGCGGCCGCT CTAGAACTAG TGGATCCCCC GGGCTGCAGG AATTCGATCA GGATCCATCG
  121 ATGCTTAGGA GGTCATATGC CATTTGGAAT AGACAACACC GACTTCACTG TCCTGGCGGG
  181 GCTAGTGCTT GCCGTGCTAC TGTACGTAAA GAGAAACTCC ATCAAGGAAC TGCTGATGTC
  241 CGATGACGGA GATATCACAG CTGTCAGCTC GGGCAACAGA GACATTGCTC AGGTGGTGAC
  301 CGAAAACAAC AAGAACTACT TGGTGTTGTA TGCGTCGCAG ACTGGGACTG CCGAGGATTA
  361 CGCCAAAAAG TTTTCCAAGG AGCTGGTGGC CAAGTTCAAC CTAAACGTGA TGTGCGCAGA
  421 TGTTGAGAAC TACGACTTTG AGTCGCTAAA CGATGTGCCC GTCATAGTCT CGATTTTTAT
  481 CTCTACATAT GGTGAAGGAG ACTTCCCCGA CGGGGCGGTC AACTTTGAAG ACTTTATTTG
  541 TAATGCGGAA GCGGGTGCAC TATCGAACCT GAGGTATAAT ATGTTTGGTC TGGGAAATTC
  601 TACTTATGAA TTCTTTAATG GTGCCGCCAA GAAGGCCGAG AAGCATCTCT CCGCCGCGGG
  661 CGCTATCAGA CTAGGCAAGC TCGGTGAAGC TGATGATGGT GCAGGAACTA CAGACGAAGA
  721 TTACATGGCC TGGAAGGACT CCATCCTGGA GGTTTTGAAA GACGAACTGC ATTTGGACGA
  781 ACAGGAAGCC AAGTTCACCT CTCAATTCCA GTACACTGTG TTGAACGAAA TCACTGACTC
  841 CATGTCGCTT GGTGAACCCT CTGCTCACTA TTTGCCCTCG CATCAGTTGA ACCGCAACGC
  901 AGACGGCATC CAATTGGGTC CCTTCGATTT GTCTCAACCG TATATTGCAC CCATCGTGAA
  961 ATCTCGCGAA CTGTTCTCTT CCAATGACCG TAATTGCATC CACTCTGAAT TTGACTTGTC
 1021 CGGCTCTAAC ATCAAGTACT CCACTGGTGA CCATCTTGCT GTTGGCCTTC CAACCCATTT
 1081 GGAAAAGGTC GACAGTTCTT ATCCATATTT CACCCTGGCA CCTGGAATCA TTTTTGACTT
1141 GGAAGCCCTG ATCCCAACGT TCCAATGCCT TCCGCGCTGA ACCTACCTAT TTAGC
//
Primer: Reverse2 ()
Region: insert 3'
Sequence file: RDB16760_A8K1b.seq check
>D05837A8_A8K1_2_reverse2_E03_15_ABI24.ab1
    1 AGGTGACAAT GATTAACGCA AAGCGCGCAA TTAACCCTCA CTAAAGGGAA CAAAAGCTGG
   61 GTACCGGGCC CCCCCTCGAG GTCGACGGTA TCGATAAGCT TGAGGAAGCT TTTACCAGAC
  121 ATCTTCTTGG TATCTACCTG AAGTCTTGAG CATCTTGATT AGCTCTGTTG CTTCATCAGT
  181 GGTAATGGAT TTACCACGGG ATAAGATGCC AACCAATGCG GTTGACACAC CCTTGGCCAT
  241 ACCCTTTGCA TCACCACAGA CGTAGATAAA TGCACCGTTG TTAATCATTT CAAATACTTG
  301 GTCTTCGTAA TCCTTTAATT TATCTTGAAC ATAAACTTTT TTGGTGTTTG GCAACCTGGA
  361 ATGGGCCACG ACCATTTCGA ACGAACCATC CAATTTTTTG GCGTATTCTG GCCATTCGTC
  421 CTGGTACAAG AAATCATCAG TGTTACGGGA TCCATAAAAC AGTATATGCT TACCTAGCGA
  481 AACGTTGTTA CCGCCCTTCT TTTGTGATTC GAGGAACGCG ACACGCTCTC TGATAAACCC
  541 ACGGAATGGG GCAACACCGG TACCTGGACC GATCATGATA ACTGGGGTGG AAGGGTTGGA
  601 AGGCAATCTG AAGTTAGAAC GACGAACGTG GACGGGCAAT TTGTAATTGG CGAAAAGTTT
  661 ACGTGGGCCA TTTAAATCGT AGTGAACAGG TAGGTTAGTT TCGGCAATGT TAACATTGTT
  721 TTGAGCCAAT TGAATGTTTC TTAACAAGTT AGTCGTAACA CCAACAACTG GAGGAGCATC
  781 AGGCAATTCT GGGTTAGGAA AGTTTTCCAC AATGGAGGTG ACATGGACGG TTTGCTTTTC
  841 AGACAGAGAA GAGGAAGAGA TAGAGTAGTA ACGAGGAGTC ATTTGGGGAA CTGATTCGAC
  901 CAAGAATTGC ATGGGTACGG TGTCCCATTT GGCGCCATCA GACAAATATT TCAGAGCATC
  961 TGCGATGTTG AAATATTTGG AGGTTATCTC GACGGCGAAT TGGTCCTTGT CTTTCGAAAG
 1021 CAGAGTCAAT TTTTTCCTTG ACGTCAGCGT TGGGGGCGAA CTGAATCAAA AGATGAAAAC
 1081 CATTGTCTGG GAGACAGGTC CTGTATTTTC AAATAGTGTT TAATAGCAGC GCCAATAGTA
 1141 GTGCCGTGGA GGGCACTTTG ACGGTGGGAT CCAGGGGCTT CAAGGTCAAA AAAATTGGGT
1201 C
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Kitahama, Y., The construction and characterization of self-sufficient lanosterol 14-demethylase fusion proteins consisting of yeast CYP51 and its reductase. Biol. Pharm. Bull. 32 (4): 558-563 (2009). PMID 19336884. [link to RRC of NBRP]