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Yeast NADPH-P450 reductase/pBKS-

Cloning vector of S. cerevisiae NADPH-P450 reductase.

Catalog number RDB16760
Resource name Yeast NADPH-P450 reductase/pBKS-
Clone info. Cloning vector of S. cerevisiae NADPH-P450 reductase.
Comment Known differences (at least): substitutions G to A (Pro, synonym) at 641nt, A to C (Asn to Thr) at 1903nt and G to T (Gly to Val) at 2056nt of CP036477.1.
Vector backbone pBluescript KS- (plasmid)
Size of vector backbone 3.0 kb
Selectable markers Amp^r
Gene/insert name Saccharomyces cerevisiae NADPH-P450 reductase cDNA
Depositor|Developer Aoyama, Yuri |
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External Database
Saccharomyces cerevisiae NADPH-P450 reductase

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Kitahama, Y et al. Biol. Pharm. Bull. 32(4), 558-63, 2009) is requested.
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Kitahama, Y et al. Biol. Pharm. Bull. 32(4), 558-63, 2009)の引用を必要とする。
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          RDB16760 Yeast NADPH-P450 reductase/pBKS- DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Kitahama, Y., The construction and characterization of self-sufficient lanosterol 14-demethylase fusion proteins consisting of yeast CYP51 and its reductase. Biol. Pharm. Bull. 32 (4): 558-563 (2009). PMID 19336884.

          Further references such as user reports and related articles (go to bottom)

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          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB16760_A8K1p1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: M13_-40
          Region: insert 5'
          Sequence file: RDB16760_A8K1a.seq check
          >D05837A8_A8K1_2_M13_-40_D03_12_ABI24.ab1
              1 AAGCCAATTG GCGCGCGGTA ATACGACTCA CTATAGGGCG AATTGGAGCT CCACCGCGGT
             61 GGCGGCCGCT CTAGAACTAG TGGATCCCCC GGGCTGCAGG AATTCGATCA GGATCCATCG
            121 ATGCTTAGGA GGTCATATGC CATTTGGAAT AGACAACACC GACTTCACTG TCCTGGCGGG
            181 GCTAGTGCTT GCCGTGCTAC TGTACGTAAA GAGAAACTCC ATCAAGGAAC TGCTGATGTC
            241 CGATGACGGA GATATCACAG CTGTCAGCTC GGGCAACAGA GACATTGCTC AGGTGGTGAC
            301 CGAAAACAAC AAGAACTACT TGGTGTTGTA TGCGTCGCAG ACTGGGACTG CCGAGGATTA
            361 CGCCAAAAAG TTTTCCAAGG AGCTGGTGGC CAAGTTCAAC CTAAACGTGA TGTGCGCAGA
            421 TGTTGAGAAC TACGACTTTG AGTCGCTAAA CGATGTGCCC GTCATAGTCT CGATTTTTAT
            481 CTCTACATAT GGTGAAGGAG ACTTCCCCGA CGGGGCGGTC AACTTTGAAG ACTTTATTTG
            541 TAATGCGGAA GCGGGTGCAC TATCGAACCT GAGGTATAAT ATGTTTGGTC TGGGAAATTC
            601 TACTTATGAA TTCTTTAATG GTGCCGCCAA GAAGGCCGAG AAGCATCTCT CCGCCGCGGG
            661 CGCTATCAGA CTAGGCAAGC TCGGTGAAGC TGATGATGGT GCAGGAACTA CAGACGAAGA
            721 TTACATGGCC TGGAAGGACT CCATCCTGGA GGTTTTGAAA GACGAACTGC ATTTGGACGA
            781 ACAGGAAGCC AAGTTCACCT CTCAATTCCA GTACACTGTG TTGAACGAAA TCACTGACTC
            841 CATGTCGCTT GGTGAACCCT CTGCTCACTA TTTGCCCTCG CATCAGTTGA ACCGCAACGC
            901 AGACGGCATC CAATTGGGTC CCTTCGATTT GTCTCAACCG TATATTGCAC CCATCGTGAA
            961 ATCTCGCGAA CTGTTCTCTT CCAATGACCG TAATTGCATC CACTCTGAAT TTGACTTGTC
           1021 CGGCTCTAAC ATCAAGTACT CCACTGGTGA CCATCTTGCT GTTGGCCTTC CAACCCATTT
           1081 GGAAAAGGTC GACAGTTCTT ATCCATATTT CACCCTGGCA CCTGGAATCA TTTTTGACTT
          1141 GGAAGCCCTG ATCCCAACGT TCCAATGCCT TCCGCGCTGA ACCTACCTAT TTAGC
          //
          Primer: Reverse2
          Region: insert 3'
          Sequence file: RDB16760_A8K1b.seq check
          >D05837A8_A8K1_2_reverse2_E03_15_ABI24.ab1
              1 AGGTGACAAT GATTAACGCA AAGCGCGCAA TTAACCCTCA CTAAAGGGAA CAAAAGCTGG
             61 GTACCGGGCC CCCCCTCGAG GTCGACGGTA TCGATAAGCT TGAGGAAGCT TTTACCAGAC
            121 ATCTTCTTGG TATCTACCTG AAGTCTTGAG CATCTTGATT AGCTCTGTTG CTTCATCAGT
            181 GGTAATGGAT TTACCACGGG ATAAGATGCC AACCAATGCG GTTGACACAC CCTTGGCCAT
            241 ACCCTTTGCA TCACCACAGA CGTAGATAAA TGCACCGTTG TTAATCATTT CAAATACTTG
            301 GTCTTCGTAA TCCTTTAATT TATCTTGAAC ATAAACTTTT TTGGTGTTTG GCAACCTGGA
            361 ATGGGCCACG ACCATTTCGA ACGAACCATC CAATTTTTTG GCGTATTCTG GCCATTCGTC
            421 CTGGTACAAG AAATCATCAG TGTTACGGGA TCCATAAAAC AGTATATGCT TACCTAGCGA
            481 AACGTTGTTA CCGCCCTTCT TTTGTGATTC GAGGAACGCG ACACGCTCTC TGATAAACCC
            541 ACGGAATGGG GCAACACCGG TACCTGGACC GATCATGATA ACTGGGGTGG AAGGGTTGGA
            601 AGGCAATCTG AAGTTAGAAC GACGAACGTG GACGGGCAAT TTGTAATTGG CGAAAAGTTT
            661 ACGTGGGCCA TTTAAATCGT AGTGAACAGG TAGGTTAGTT TCGGCAATGT TAACATTGTT
            721 TTGAGCCAAT TGAATGTTTC TTAACAAGTT AGTCGTAACA CCAACAACTG GAGGAGCATC
            781 AGGCAATTCT GGGTTAGGAA AGTTTTCCAC AATGGAGGTG ACATGGACGG TTTGCTTTTC
            841 AGACAGAGAA GAGGAAGAGA TAGAGTAGTA ACGAGGAGTC ATTTGGGGAA CTGATTCGAC
            901 CAAGAATTGC ATGGGTACGG TGTCCCATTT GGCGCCATCA GACAAATATT TCAGAGCATC
            961 TGCGATGTTG AAATATTTGG AGGTTATCTC GACGGCGAAT TGGTCCTTGT CTTTCGAAAG
           1021 CAGAGTCAAT TTTTTCCTTG ACGTCAGCGT TGGGGGCGAA CTGAATCAAA AGATGAAAAC
           1081 CATTGTCTGG GAGACAGGTC CTGTATTTTC AAATAGTGTT TAATAGCAGC GCCAATAGTA
           1141 GTGCCGTGGA GGGCACTTTG ACGGTGGGAT CCAGGGGCTT CAAGGTCAAA AAAATTGGGT
          1201 C
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Kitahama, Y., The construction and characterization of self-sufficient lanosterol 14-demethylase fusion proteins consisting of yeast CYP51 and its reductase. Biol. Pharm. Bull. 32 (4): 558-563 (2009). PMID 19336884.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl