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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pDEST/mCherry-TubbyC

Expression vector of human Tubby proteins C-terminal tubby domain (Tubby C).

Catalog number RDB16766
Resource name pDEST/mCherry-TubbyC
Clone info. Expression vector of human Tubby proteins C-terminal tubby domain (Tubby C) fused with mCherry at N-terminus.
Comment Expression was confirmed by the depositor with immunofluorescence. Insert gene, from HeLa cell cDNA by PCR amplification. Vector, amplified mCherry was cloned into expression vector pcDNA-DEST40, generating DEST40-mCherry.
Vector backbone pcDNA-DEST40 (plasmid)
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells).
Gene/insert name human TUB cDNA
Depositor|Developer Sako, Yasushi | Abe, Mitsuhiro |
Other clones in our bank

External Database
human TUB

          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Abe, M et al. Mol. Cell Biol. 32(8):1396−1407, 2012) is requested.
          Ordering Please visit Information of Request for Distribution.[link] 
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          Remarks
          This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.
          Gateway® Expression clone. Please refer Life Technologies Corporation signs license agreement with RIKEN BRC.
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Abe, M et al. Mol. Cell Biol. 32(8):1396−1407, 2012)の引用を必要とする。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          備考
          Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。
          Gateway®テクノロジーを用いた発現クローンです。「Gateway®テクノロジーを用いたクローンの提供と寄託のご案内」をご覧ください。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB16766 pDEST/mCherry-TubbyC DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The pDEST/mCherry-TubbyC was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16766).

          Reference section:

          Abe, M., Makino, A., Hullin-Matsuda, F., Kamijo, K., Ohno-Iwashita, Y., Hanada, K., Mizuno, H., Miyawaki, A., Kobayashi, T., A role for sphingomyelin-rich lipid domains in the accumulation of phosphatidylinositol-4,5-bisphosphate to the cleavage furrow during cytokinesis. Mol. Cell. Biol. 32 (8): 1396-1407 (2012). PMID 22331463.

          Further references such as user reports and related articles (go to bottom)


          References and tips

          Featured content


          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB16766_A8Kmp1-2.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV_Forward (Pr0016)
          Region: CMV pro,T7 pro,insert 5'(mCherry 5')
          Sequence file: RDB16766_A8Kme.seq check
          >D05982A3_A8Km_1_CMV_Forward_A09_03_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNTT GNNGCNNTGG GCGGTAGGCG TGTACGGTGG GAGGTCTATA
             61 TAAGCAGAGC TCTCTGGCTA ACTAGAGAAC CCACTGCTTA CTGGCTTATC GAAATTAATA
            121 CGACTCACTA TAGGGAGACC CAAGCTGGCT AGTTAAGCTA TCAGGTACCA TGGTGAGCAA
            181 GGGCGAGGAG GATAACATGG CCATCATCAA GGAGTTCATG CGCTTCAAGG TGCACATGGA
            241 GGGCTCCGTG AACGGCCACG AGTTCGAGAT CGAGGGCGAG GGCGAGGGCC GCCCCTACGA
            301 GGGCACCCAG ACCGCCAAGC TGAAGGTGAC CAAGGGTGGC CCCCTGCCCT TCGCCTGGGA
            361 CATCCTGTCC CCTCAGTTCA TGTACGGCTC CAAGGCCTAC GTGAAGCACC CCGCCGACAT
            421 CCCCGACTAC TTGAAGCTGT CCTTCCCCGA GGGCTTCAAG TGGGAGCGCG TGATGAACTT
            481 CGAGGACGGC GGCGTGGTGA CCGTGACCCA GGACTCCTCC CTGCAGGACG GCGAGTTCAT
            541 CTACAAGGTG AAGCTGCGCG GCACCAACTT CCCCTCCGAC GGCCCCGTAA TGCAGAAGAA
            601 GACCATGGGC TGGGAGGCCT CCTCCGAGCG GATGTACCCC GAGGACGGCG CCCTGAAGGG
            661 CGAGATCAAG CAGAGGCTGA AGCTGAAGGA CGGCGGCCAC TACGACGCTG ANGTCAAGAC
            721 CACCTACAAG GCCAAGAAGC CCGTGCAGCT GCCCGGCGCC TACAACGTCA ACATCAAGTT
            781 GGACATCACC TCCCACAACG AGGNNTACAC CATCGTGGAA CAGTACGAAC GCGCCGAGGG
            841 CCGCCACTCC ACCGGCGGCA TGGANGANCT GTACAAGGGT GGANGTACCA CTAGNGATGA
            901 NGANGANAAT AGCTNNNGCT NCTNCCANCN AAATAGTAAC ACCCGCCCCA GCNNNGCTAC
            961 TANCNNNANN NNCGNCNNNN NNNAGNCNNN AGCCCNNAGC CNNNCNGCTN NNNANCNNNN
          1021 NNNNNN
          //
          Primer: f1ori_F (Pr0280)
          Region: f1 ori,bGH pA,insert 3'(TubbyC 3')
          Sequence file: RDB16766_A8Kmf.seq check
          >D05982A3_A8Km_1_f1ori_F_C11_03_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NGCGGTCNCG CTGCGCGTAA CCACCACACC
             61 CGCCGCGCTT AATGCGCCGC TACAGGGCGC GTGGGGATAC CCCCTAGAGC CCCAGCTGGT
            121 TCTTTCCGCC TCAGAAGCCA TAGAGCCCAC CGCATCCCCA GCATGCCTGC TATTGTCTTC
            181 CCAATCCTCC CCCTTGCTGT CCTGCCCCAC CCCACCCCCC AGAATAGAAT GACACCTACT
            241 CAGACAATGC GATGCAATTT CCTCATTTTA TTAGGAAAGG ACAGTGGGAG TGGCACCTTC
            301 CAGGGTCAAG GAAGGCACGG GGGAGGGGCA AACAACAGAT GGCTGGCAAC TAGAAGGCAC
            361 AGTCGAGGCT GATCAGCGGG TTTAAACGGA TCTCTACTCG CACGCCAGCT TGCTGTCGAA
            421 GCTGGACAGG GCAATGGCAA AGGCCTGCAG TGCACACAGC GGGTAGTTGT AATCCATGGT
            481 GAACACATCC TCTGCTACCC GGCCAAACTG CATCACGATG TAGTCCGGGT CATTGCCATG
            541 GATGATCTGG AAGTTCTTCA CGGAGGCCTG TGTGACGCGC CCATGGAAGT TGAGTACATA
            601 GGACTGTGTG TCATCATTCC AGACAGGTGT CTTGTTTTGC AGCTCGATGA TACTCTCCGT
            661 GTTCTTATTC TGCCAGCGTG CTAGCAGTGT CTCATGCTCG TTGCGGGGGC GGATAGAGAC
            721 TCTCTCATGA ACCATGTTCA TGCCTGGGAC AATCACGCTC ATCTTCCGAG GCCCCTTGAA
            781 GCCTAAGACG TTTGTCTCGT ANCACACAGC TGCCAGCTCC TGACGTNAGG NTCCACTTTC
            841 CAAGTGGANG ANGANGNCTT CTGAGGGTTG ANNNNNTTGN CNNNNACNGT GAACTTGGTG
            901 NCCATCANGT TGGNNCGCAG TTNNCCNANA NNNCTGTCCC CNCCTCNAGN CAANTCTGTT
            961 GGNNCCACAN ANATGANGTN ANTNGNAANT TTTANNCTNN TTTCTCTTNN TTCCNNNNNN
          1021 GNANNANNNN NNTNNNNNCN NNNNNNNNN
          //
          Primer: mRFP1-inner1 (Pr0443)
          Region: insert mid(mCherry 3',TubbyC 5')
          Sequence file: RDB16766_A8Kmg.seq check
          >16766_16766_B3CdF_mRFP1-inner1_D01_04_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNGNN TCCTCCCTGC AGGANGGCGA GTTCATCTAC AAGGTGAAGC
             61 TGCGCGGCAC CAACTTCCCC TCCGACGGCC CCGTAATGCA GAAGAAGACC ATGGGCTGGG
            121 AGGCCTCCTC CGAGCGGATG TACCCCGAGG ACGGCGCCCT GAAGGGCGAG ATCAAGCAGA
            181 GGCTGAAGCT GAAGGACGGC GGCCACTACG ACGCTGAGGT CAAGACCACC TACAAGGCCA
            241 AGAAGCCCGT GCAGCTGCCC GGCGCCTACA ACGTCAACAT CAAGTTGGAC ATCACCTCCC
            301 ACAACGAGGA CTACACCATC GTGGAACAGT ACGAACGCGC CGAGGGCCGC CACTCCACCG
            361 GCGGCATGGA CGAGCTGTAC AAGGGTGGAG GTACCACTAG TGATGAGGAG GAGAATAGCT
            421 CCAGCTCCTC CCAGCTAAAT AGTAACACCC GCCCCAGCTC TGCTACTAGC AGGAAGTCCG
            481 TCAGGGAGGC AGCCTCAGCC CCTAGCCCAA CAGCTCCAGA GCAACCAGTG GACGTTGAGG
            541 TCCAGGATCT TGAGGAGTTT GCACTGAGGC CGGCCCCCCA GGGTATCACC ATCAAATGCC
            601 GCATCACTCG GGACNAGAAA GGGATGGANC GGGGCATGTA CCCCACCTAC TTTCTGCACC
            661 TGNANCGTGA NGNNGGGNNN ANNNGNTCNT NCTNNNGGNN NNNNNANNNN NNNNNTAAAN
          721 NNTNNNNNNN NNN
          //
          Primer: SV40pro_F (Pr0110)
          Region: SV40 pro_ori,NeoR
          Sequence file: RDB16766_A8Kmh.seq check
          >D05982A3_A8Km_1_SV40pro_F_D09_12_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NANNNGTGAG GANGNTTTTT TGGAGGCCTA GGCTTTTGCA
             61 AAAAGCTCCC GGGAGCTTGT ATATCCATTT TCGGATCTGA TCAAGAGACA GGATGAGGAT
            121 CGTTTCGCAT GATTGAACAA GATGGATTGC ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA
            181 GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG CTCTGATGCC GCCGTGTTCC
            241 GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC CGACCTGTCC GGTGCCCTGA
            301 ATGAACTGCA GGACGAGGCA GCGCGGCTAT CGTGGCTGGC CACGACGGGC GTTCCTTGCG
            361 CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG GCTGCTATTG GGCGAAGTGC
            421 CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA GAAAGTATCC ATCATGGCTG
            481 ATGCAATGCG GCGGCTGCAT ACGCTTGATC CGGCTACCTG CCCATTCGAC CACCAAGCGA
            541 AACATCGCAT CGAGCGAGCA CGTACTCGGA TGGAAGCCGG TCTTGTCGAT CAGGATGATC
            601 TGGACGAAGA GCATCAGGGG CTCGCGCCAG CCGAACTGTT CGCCAGGCTC AAGGCGCGCA
            661 TGCCCGACGG CGAGGATCTC GTCGTGACCC ATGGCGATGC CTGCTTGCCG AATATCATGG
            721 TGGAAAATGG CCGCTTTTCT GGATTCATCG ACTGTGGCCG GCTGGGTGTG GCGGANCGCT
            781 ATCAGGACAT AGCGTTGGCT ACCCGTGATA TTGCTGAAGA GCTTGGCGGC GAATGNNCTG
            841 ANCGCTTCCT CGTGCTTTAC GGTATCGCCG CTCCCGATTC NCANCGCATC GCCTTCTATC
            901 GCCTTCTTGA CGAGTNNTNC TGANCNGGNN NCTGGGGNNN GCGAAATGAC NNANCNANCG
          961 ACGCCNNANC NNGNNNTCNC NNNNNTNNNA NTNNNNNNNN NN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Abe, M., A role for sphingomyelin-rich lipid domains in the accumulation of phosphatidylinositol-4,5-bisphosphate to the cleavage furrow during cytokinesis. Mol. Cell. Biol. 32 (8): 1396-1407 (2012). PMID 22331463.

          2023.05.01

          GNP_filter3_RDBDEP_html_230501.pl