Resource data sheet
Prev.
Please review the HTS results indicated by check icon below as well as clone information before placing your order.

pCaMKIIa-C1V1-Venus

Mammalian expression vector encoding a chimeric channelrhodopsin (C1V1).

Catalog number RDB16795
Resource name pCaMKIIa-C1V1-Venus
Clone info. Mammalian expression vector encoding a chimeric channelrhodopsin (C1V1) tagged with Venus at the C-terminus under CaMKIIa promoter.
Comment C1V1 consists of Met1-Leu164 of channelrhodopsin 1 from Chlamydomonas reinhardtii (CrChR1) and Leu121-Asp300 of channelrhodopsin 1 from Volvox carteri (VcChR1). Expression was confirmed by the depositor with whole-cell patch-clamp recording.
Vector backbone pCaMKIIa-Venus (plasmid)
Size of vector backbone 5.4 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells).
Gene/insert name Chlamydomonas reinhardtii acop1 (CrChR1) cDNA Volvox carteri VcChR1 cDNA
Depositor|Developer Ishizuka, Toru |
Other clones in our bank

External Database
Chlamydomonas reinhardtii acop1 (CrChR1) Volvox carteri VcChR1

          Reference sequence
            AB058890,EU622855
           
          Sequence (full) RDB16795hts01.seqcheck assembled from experimentally sequenced data
          Test sheet RDB16795_A8L5p1-1.pdf 

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Hososhima, S. et al., Sci. Rep. 5: 16533, 2015) For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
          Ordering
          Order form [Credit Card Payment]  [Bank Transfer Payment]
          MTA, for use for not-for-profit academic purpose [Word]
          MTA, for use for for-profit purpose [Word]
          Please visit Information of Request for Distribution.[link] 
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Hososhima, S. et al., Sci. Rep. 5: 16533, 2015)。営利機関の利用希望者は、事前に利用条件等につき寄託者と合意し、提供承諾を得ること。
          提供依頼
          提供依頼書 [Word]
          提供同意書 (MTA, 非営利学術目的用)[Word]
          提供同意書 (MTA, 営利目的用)[Word]
          手続きの概要は、「提供申込みについて[link]」をご覧ください。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB16795 pCaMKIIa-C1V1-Venus DNA solution


          How to cite this biological resource

          Materials & Methods section:

          The pCaMKIIa-C1V1-Venus was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16795).

          Reference section:

          Hososhima, S., Yuasa, H., Ishizuka, T., Hoque, M.R., Yamashita, T., Yamanaka, A., Sugano, E., Tomita, H., Yawo, H., Near-infrared (NIR) up-conversion optogenetics. Sci. Rep. 5: 16533 (2015). PMID 26552717. [link to RRC of NBRP]

          Further references such as user reports and related articles (go to bottom)


          Tips

          Featured content

          Featured content Genetic Resources for Optgenetics Research (English text)

          References

          Original, user report and related articles

          original Hososhima, S., Near-infrared (NIR) up-conversion optogenetics. Sci. Rep. 5: 16533 (2015). PMID 26552717. [link to RRC of NBRP]

          2024.03.26

          HTML_filter_RDB_240326.pl