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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pMK392 (mAID-mClover-BSD)

A plasmid for construction of C-terminal mAID-mClover-BSD tagging donors.

Catalog number RDB16820
Resource name pMK392 (mAID-mClover-BSD)
Clone info. A plasmid for construction of C-terminal mAID-mClover-BSD tagging donors.
Vector backbone pBluescript (plasmid)
Size of vector backbone 2.9 kb
Selectable markers Am^r (E. coli)
Gene/insert name Arabidopsis thaliana AtIAA17 cDNA Aequorea victoria GFP cDNA
Depositor|Developer Kanemaki, Masato |
Other clones in our bank

External Database
Arabidopsis thaliana AtIAA17 Aequorea victoria GFP

          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019)。 学術研究にのみ限定、商業営利目的利用を禁ずる。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に相談すること。
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          手続きの概要は、「提供申込みについて[link]」をご覧ください。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB16820 pMK392 (mAID-mClover-BSD) DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The pMK392 (mAID-mClover-BSD) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16820).

          Reference section:

          Yesbolatova, A., Natsume, T., Hayashi, K.I., Kanemaki, M.T., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591. [link to RRC of NBRP]

          Further references such as user reports and related articles (go to bottom)


          Tips

          Featured content

          Featured content Auxin Inducible Degron (AID) System (English text)
          Featured content Auxin Inducible Degron (AID) System (Japanese text)

          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB16820_A8Lpp1-3.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pUC-M13_-100 (Pr0310)
          Region: T7 pro,insert 5'(Linker,mAID, mClover 5')
          Sequence file: RDB16820_A8Lpd.seq check
          >D06157B7_A8Lp_1_pUC-M13_-100_A07_01_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NGGGNNNGCN NGGNNTTTCC CNGTNNCGAC GTTGTAAAAC
             61 GACGGCCAGT GAGCGCGCGT AATACGACTC ACTATAGGGC GAATTGGAGC TCCCCGGGAT
            121 CCGGTGCAGG CGCCAAGGAG AAGAGTGCTT GTCCTAAAGA TCCAGCCAAA CCTCCGGCCA
            181 AGGCACAAGT TGTGGGATGG CCACCGGTGA GATCATACCG GAAGAACGTG ATGGTTTCCT
            241 GCCAAAAATC AAGCGGTGGC CCGGAGGCGG CGGCGTTCGT GAAGGTATCA ATGGACGGAG
            301 CACCGTACTT GAGGAAAATC GATTTGAGGA TGTATAAAGC TAGCATGGTG AGCAAGGGCG
            361 AGGAGCTGTT CACCGGGGTG GTGCCCATCC TGGTCGAGCT GGACGGCGAC GTAAACGGCC
            421 ACAAGTTCAG CGTCCGCGGC GAGGGCGAGG GCGATGCCAC CAACGGCAAG CTGACCCTGA
            481 AGTTCATCTG CACCACCGGC AAGCTGCCCG TGCCCTGGCC CACCCTCGTG ACCACCTTCG
            541 GCTACGGCGT GGCCTGCTTC AGCCGCTACC CCGACCACAT GAAGCAGCAC GACTTCTTCA
            601 AGTCCGCCAT GCCCGAAGGC TACGTCCAGG AGCGCACCAT CTCTTTCAAG GACGACGNNN
            661 NNNACAAGAC CCGCGCCGAN GTGAAGTTCG AGGGCGACAC CCTGGTGAAC CGCATCGAGC
            721 TGAANGGCAT CNNNTTCAAG GAGGANGGCA ACATCCTGGG GCACAAGCTG GAGTACAACT
            781 TCAACAGCCA CAACGTCTAT ATCACGGCCG ANAAGCANAA NAANGGCATC NANGCTAANT
            841 TNNAGATCCG CCACAACGTT NNNGGANNGN ANCGTGCAGC TCGCCNANCA NNTACNANCA
            901 NAACNCCCCC NNTCGNNGAN NGNCCCCGTG CNNCTGCCCG ANNNNNNCNA CCTGAGCNNT
          961 NNNNNCNNNN CTGNNNNNAN NNNNNCNNNN NANNNNNNNN NN
          //
          Primer: lacIpro-B_F (Pr0724)
          Region: T3 pro,insert 3'(loxP,mPGK ter 3')
          Sequence file: RDB16820_A8Lpe.seq check
          >16820_16820_B3C9F_lacIpro-B_F_H02_23_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN CGNANCNNNG TCNGTGAGCG AGGAANNCNN GAAGAGCGCC
             61 CAATACGCAA ACCGCCTCTC CCCGCGCGTT GGCCGATTCA TTAATGCAGC TGGCACGACA
            121 GGTTTCCCGA CTGGAAAGCG GGCAGTGAGC GCAACGCAAT TAATGTGAGT TAGCTCACTC
            181 ATTAGGCACC CCAGGCTTTA CACTTTATGC TTCCGGCTCG TATGTTGTGT GGAATTGTGA
            241 GCGGATAACA ATTTCACACA GGAAACAGCT ATGACCATGA TTACGCCAAG CGCGCAATTA
            301 ACCCTCACTA AAGGGAACAA AAGCTGGGTA CCGGATCCTA GTGAACCTCT TCGAGGGACC
            361 TAATAACTTC GTATAGCATA CATTATACGA AGTTATATTA AGGGTTCCGG ATCTCGACCA
            421 GCTTCTGATG GAATTAGAAC TTGGCAAAAC AATACTGAGA ATGAAGTGTA TGTGGAACAG
            481 AGGCTGCTGA TCTCGTTCTT CAGGCTATGA AACTGACACA TTTGGAAACC ACAGTACTTA
            541 GAACCACAAA GTGGGAATCA AGAGAAAAAC AATGATCCNN CNAGAGATCT ATAGATCTAT
            601 AGATCATGAG TGNGAGGAAT GAGCTGGNCC TTAATTTGNN TTTGCTNGNT TAAATTATGA
            661 TATCCAACTA TGAAANATTA TCATAAAGCN ATAGTAAAGA GCNNTCANTA AAGANNNNNN
            721 ATTTATCNNA TCCCNCCCCA CCCCCNCCCC NNNANCTNCN NTNNNTNNAT TNAAATGNNN
            781 GNNACTCNNN NNNNNNCNNN NNTNANAANN NNGNNAGNAA AAANNTNNNT TTTNNNGNNN
          841 NNNNTNNNTG NTNANNNNNA TNNTNNNNTN NNNNNNNCNN GGNNNNNNNN NN
          //
          Primer: PGK_N_rev (Pr0243)
          Region: insert mid(loxP,SV40 pA,mClover 3')
          Sequence file: RDB16820_A8Lpf.seq check
          >D06157B7_A8Lp_1_PGK_N_rev_C07_07_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNGGNNNANT TCGAGGTCGA GNTCCTAAGC TTGGCTGGAC
             61 GTAAACTCCT CTTCAGACCT AATAACTTCG TATAGCATAC ATTATACGAA GTTATATTAA
            121 GGGTTATTGA ATATGATCGA CGCGTTAAGA TACATTGATG AGTTTGGACA AACCACAACT
            181 AGAATGCAGT GAAAAAAATG CTTTATTTGT GAAATTTGTG ATGCTATTGC TTTATTTGTA
            241 ACCATTATAA GCTGCAATAA ACAAGTTAAC AACAACAATT GCATTCATTT TATGTTTCAG
            301 GTTCAGGGGG AGGTGTGGGA GGTTTTTTAA AGCAAGTAAA ACCTCTACAA ATGTGGTATG
            361 GCTGATTATG ATCAGTTATC TAGATTACTT GTACAGCTCG TCCATGCCAT GTGTAATCCC
            421 GGCGGCGGTC ACGAACTCCA GCAGGACCAT GTGATCGCGC TTCTCGTTGG GGTCTTTGCT
            481 CAGCTTGGAC TGATGGCTCA GGTAGTGGTT GTCGGGCAGC AGCACGGGGC CGTCGCCGAT
            541 GGGGGTGTTC TGCTGGTAGT GGTCGGCGAG CTGCACGCTG CCGTCCTCAA CGTTGTGGCG
            601 GATCTTGAAG TTAGCCTTGA TGCCGTTCTT CTGCTTGTCG GCCGTGATAT AGACGTTGTG
            661 GCTGTTGAAG TTGTACTCCA GCTTGTGCCC CANGATGTTG CCGTCCTCCT TGAAGTCGAT
            721 GCCCTTCAGC TCGATGCGGT TCANCAGGGT GTCGCCCTCG AACTTCACCT CGGCGCGGGT
            781 CTTGTANGTA CCGTCNTCCT TGAAAGANAT GGTGCGCTCC TGGACGTAGC CTTCNGGCAT
            841 GGCGGANTTG NAANAAGTCN NGCTGCTNNA TGTGGTNNNG GNANCGGCTN AANCNNNNNN
          901 NCNNNNNNCN ANNNNNNCAC NAGGGNGGGN NAGGGNANGG GCAGCTNNNN NNNNNN
          //
          Primer: PGKpro_F (Pr0249)
          Region: insert mid(BSD,mPGK ter 5')
          Sequence file: RDB16820_A8Lpg.seq check
          >D06157B7_A8Lp_1_PGKpro_F_D07_10_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNTCTCCN GGCCTTTCGN CCTGCATCCN TCTAGATCTC
             61 GAGCCACCAT GGCCAAGCCT TTGTCTCAAG AAGAATCCAC CCTCATTGAA AGAGCAACGG
            121 CTACAATCAA CAGCATCCCC ATCTCTGAAG ACTACAGCGT CGCCAGCGCA GCTCTCTCTA
            181 GCGACGGCCG CATCTTCACT GGTGTCAATG TATATCATTT TACTGGGGGA CCTTGTGCAG
            241 AACTCGTGGT GCTGGGCACT GCTGCTGCTG CGGCAGCTGG CAACCTGACT TGTATCGTCG
            301 CGATCGGAAA TGAGAACAGG GGCATCTTGA GCCCCTGCGG ACGGTGCCGA CAGGTGCTTC
            361 TCGATCTGCA TCCTGGGATC AAAGCCATAG TGAAGGACAG TGATGGACAG CCGACGGCAG
            421 TTGGGATTCG TGAATTGCTG CCCTCTGGTT ATGTGTGGGA GGGCTAATCG ATGATATCAG
            481 ATCCCCGGGA TGCAGAAATT GATGATCTAT TAAACAATAA AGATGTCCAC TAAAATGGAA
            541 GTTTTTCCTG TCATACTTTG TTAAGAAGGG TGAGAACAGA GTACCTACAT TTTGAATGGA
            601 AGGATTGGAG CTACGGGGGT GGGGGTGGGG TGGGATTAGA TAAATGCCTG CTCTTTACTG
            661 AAGGCTCTTT ACTATTGCTT TATGATAATG TTTCATAGTT GGATATCATA ATTTAAACAA
            721 GCAAAACCAA ATTAAGGGCN AGCTCATTCC TCCCACTCAT GATCTATAGA TCTATAGNTC
            781 TCTCGTGNNA TCATTGTTTT TCTCTTGATT CCCACTTTGT GGTTCTNAGT ACTGTGGTTT
            841 CNNAATGTGT CAGTTTCNTA NCNTGNAGAA CGANNTCAGC AGCCTCTGTN CCNCATNCAC
            901 TTCATTCTCA NNATTNNTTT GCCNAGTNNN NNNTCNNTCA NAANCTNGNN NNNNATNNNG
          961 NNNNNTNANN ANNNNNTNNN NNNNNNN
          //
          Primer: xFPendR2 (Pr1209)
          Region: insert mid(mAID 3',mClover 5')
          Sequence file: RDB16820_A8Lph.seq check
          >16820_16820_B3C9F_xFPendR2_H03_24_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN GNNNNTGTGN NCGCGCTNNN NNNTGGGGTC
             61 TTTGCTCAGC TNGNNNNNNN NNNGCTCAGG TAGTGGTTGT CGGGCAGCAG CACGGGGCCG
            121 TCGCCGATGG GGGTGTTCTG CTGGTAGTGG TCGGCGAGCT GCACGCTGCC GTCCTCAACG
            181 TTGTGGCGGA TCTTGAAGTT AGCCTTGATG CCGTTCTTCT GCTTGTCGGC CGTGATATAG
            241 ACGTTGTGGC TGTTGAAGTT GTACTCCAGC TTGTGCCCCA GGATGTTGCC GTCCTCCTTG
            301 AAGTCGATGC CCTTCAGCTC GATGCGGTTC ACCAGGGTGT CGCCCTCGAA CTTCACCTCG
            361 GCGCGGGTCT TGTAGGTACC GTCGTCCTTG AAAGAGATGG TGCGCTCCTG GACGTAGCCT
            421 TCGGGCATGG CGGACTTGAA GAAGTCGTGC TGCTTCATGT GGTCGGGGTA GCGGCTGAAG
            481 CAGGCCACGC CGTAGCCGAA GGTGGTCACG AGGGTGGGCC AGGGCACGGG CAGCTTGCCG
            541 GTGGTGCAGA TGAACTTCAG GGTCAGCTTG CCGTTGGTGG CATCGCCCTC GCCCTCGCCG
            601 CGGACGCTGA ACTTGTGGCC GTTTACGTCG CCGTCCAGCT CGACCAGGAT GGGCACCACC
            661 CCGGTGAACA GCTCCTCGCC CTTGCTCACC ATGCTAGCTT TATACATCCT CNAATCGATT
            721 TTCCTCAAGT ACGGTGCTCC NTCCNTTGAT ACCTTCACGA ACGCCGCCNC CTNNNGGCCA
            781 CCGCTTGNNT TTTNGNNNNN AAACCATCAC GTNCTTNCNG TATGATCTCA NCNGNGGNCN
            841 NNNCCANANT TGTGCCTTGN NNNGANNNTT NGNNTGGATC TTTNNNANAN NNCTCNTCNN
            901 NNTNNNGCNN GCNNCNNNNN CNGGGNNNNN TNNNNNNNNC CNNANANNGA GTCNNNNTNN
          961 NCNNNNNNNN NN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591. [link to RRC of NBRP]

          2024.03.26

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