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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pMK394 (mAID-Nluc-Neo)

A plasmid for construction of C-terminal mAID-Nluc-Neo tagging donors.

Catalog number RDB16822
Resource name pMK394 (mAID-Nluc-Neo)
Clone info. A plasmid for construction of C-terminal mAID-Nluc-Neo tagging donors.
Vector backbone pBluescript (plasmid)
Size of vector backbone 2.9 kb
Selectable markers Am^r (E. coli)
Gene/insert name Arabidopsis thaliana AtIAA17 cDNA Oplophorus gracilirostris luciferase cDNA
Depositor|Developer Kanemaki, Masato |
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External Database
Arabidopsis thaliana AtIAA17 Oplophorus gracilirostris luciferase

          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.
          Additional terms and conditions:
          The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019)。 学術研究にのみ限定、商業営利目的利用を禁ずる。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に相談すること。
          付加的提供条件:
          本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。
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          提供同意書 (MTA, 営利目的用)[Word]
          手続きの概要は、「提供申込みについて[link]」をご覧ください。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB16822 pMK394 (mAID-Nluc-Neo) DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The pMK394 (mAID-Nluc-Neo) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16822).

          Reference section:

          Yesbolatova, A., Natsume, T., Hayashi, K.I., Kanemaki, M.T., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591. [link to RRC of NBRP]

          Further references such as user reports and related articles (go to bottom)


          Tips

          Featured content

          Featured content Auxin Inducible Degron (AID) System (English text)
          Featured content Auxin Inducible Degron (AID) System (Japanese text)

          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB16822_A8Lpp1-2.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pUC-M13_-100 (Pr0310)
          Region: T7 pro,insert 5'(Linker,mAID,Nluc 5')
          Sequence file: RDB16822_A8Lpd.seq check
          >D06157B9_A8Lp_1_pUC-M13_-100_A08_02_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NGGGNNNGCC NGGNTTTTCC CNGTCACGNC GTTGTAAAAC
             61 GACGGCCAGT GAGCGCGCGT AATACGACTC ACTATAGGGC GAATTGGAGC TCCCCGGGAT
            121 CCGGTGCAGG CGCCAAGGAG AAGAGTGCTT GTCCTAAAGA TCCAGCCAAA CCTCCGGCCA
            181 AGGCACAAGT TGTGGGATGG CCACCGGTGA GATCATACCG GAAGAACGTG ATGGTTTCCT
            241 GCCAAAAATC AAGCGGTGGC CCGGAGGCGG CGGCGTTCGT GAAGGTATCA ATGGACGGAG
            301 CACCGTACTT GAGGAAAATC GATTTGAGGA TGTATAAAGC TAGCATGGTC TTCACACTCG
            361 AAGATTTCGT TGGGGACTGG CGACAGACAG CCGGCTACAA CCTGGACCAA GTCCTTGAAC
            421 AGGGAGGTGT GTCCAGTTTG TTTCAGAATC TCGGGGTGTC CGTAACTCCG ATCCAAAGGA
            481 TTGTCCTGAG CGGTGAAAAT GGGCTGAAGA TCGACATCCA TGTCATCATC CCGTATGAAG
            541 GTCTGAGCGG CGACCAAATG GGCCAGATCG AAAAAATTTT TAAGGTGGTG TACCCTGTGG
            601 ATGATCATCA CTTTAAGGTG ATCCTGCACT ATGGCACACT GGTAATCGAC GGGGTTACGC
            661 CGAACATGAT CGACTATTTC GGACGGCCGT ATGAAGGCAT CGCCGTGTTC GACNGCAAAA
            721 AGATCACTGT AACAGGGACC CTGTGGAACG GCAACAAAAT TATCGACGAN CGCCTGNTCA
            781 ACCCCGACNG CTCCCTGCTN TTCCGANTAA CCATCAACGG NNTGANCGGC TGGCGGCTGN
            841 GCGAACGCNT TCTGGCGNNA NCGGCCGCTC NANTCNAGAT AACTGATCAT ANTCAGCCNN
          901 ACCNCATTTN GTANNNGTTT TNNNTNNCNT NNAAANNNCN TCCNNNNNCN NNNNNNNNN
          //
          Primer: SV40pA3element Reverse (Pr0108)
          Region: T3 pro,insert 3'(TK pA,NeoR KanR)
          Sequence file: RDB16822_A8Lpe.seq check
          >16822_16822_B3C8F_SV40pA3'element Reverse_G01_07_ABI08.ab1
              1 NNNNNNNNNN NNNGNNNNNN NNNNATGNNN NNNNCCNGCA ACGCGGCCTT TTTACGGTTC
             61 CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG
            121 GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG
            181 CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCCAA TACGCAAACC GCCTCTCCCC
            241 GCGCGTTGGC CGATTCATTA ATGCAGCTGG CACGACAGGT TTCCCGACTG GAAAGCGGGC
            301 AGTGAGCGCA ACGCAATTAA TGTGAGTTAG CTCACTCATT AGGCACCCCA GGCTTTACAC
            361 TTTATGCTTC CGGCTCGTAT GTTGTGTGGA ATTGTGAGCG GATAACAATT TCACACAGGA
            421 AACAGCTATG ACCATGATTA CGCCAAGCGC GCAATTAACC CTCACTAAAG GGAACAAAAG
            481 CTGGGTACCG GATCCAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT TATTGCCGTC
            541 ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT CCCCCTAGGG
            601 TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC GGCGTCCCGG
            661 AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA ATCTCGTGAT
            721 GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT CAGAAGAACT
            781 CGTCAAGAAG GCGANAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA CCGTAAAGCA
            841 CGAGGAANCG GTCAGCCCAT TCNCCGCCAA GCTCTNCANC NATATCACGG GTAGCNAACG
            901 CNATGTCCTG ATAGCNGNCC GCCNCACCNA NCCNGNCCNC AGTCGANNNA NTNCNGAAAA
          961 NCNGNNNNTT TTNCNCCNNG ANNNTNNNNN NNNNN
          //
          Primer: SV40pro_R2 (Pr0516)
          Region: insert mid(SV40 pro_ori,AmpR pro,
          Sequence file: RDB16822_A8Lpf.seq check
          >D06157B9_A8Lp_1_SV40pro_R2_F01_06_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNCTGCCTGC TGGGGANCCT GGGGNNTTTC CNCACCCTAA
             61 CTGACACACA TTCNNCAGCT GGTTCTTTCC GCCTCAGGAC TCTTCCTTTT TCAATATTAT
            121 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA
            181 AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGCGCCCTGT
            241 AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC
            301 AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC
            361 TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG
            421 CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA
            481 TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC
            541 CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG
            601 CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT
            661 AACAAAATAT TAACGCTTAC AATTTACGCG TTAAGATACA TTGATGAGTT TGGACAAACC
            721 ACAACTAGAA TGCAGTGAAA AAAATGCTTT ATTTGTGAAA TTTGTGATGC TATTGCTTTA
            781 TTTGTAACCA TTATAAGCTG CAATAAACAA GTTAACAANN ACNATTGCAT TCATTTTATG
            841 TTTCNNGTTC AGGGGGANGT GTGGGAGNNT TTTTTAAAGC AAGTAAAANC TCTACAAATG
            901 TGNNATGGNT GATTATGANC AGTTATCTNG ACTCGAGCNG NNNGCTTANN CCAGANTGNG
          961 TTCGCNCANC CGCCAGNNNN GNCACTCCNN TNNANNGNNN NTNNGNNNNN NNNNN
          //
          Primer: SV40pro_F (Pr0110)
          Region: insert mid(SV40 pro_ori, NeoR_KanR 5')
          Sequence file: RDB16822_A8Lpg.seq check
          >D06157B9_A8Lp_1_SV40pro_F_C08_08_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNNGNN GNNGNTTTTT TGGANGCCTA GGCTTTTGCA
             61 AAGATCGATC AAGAGACAGG ATGAGGATCG TTTCGCATGA TTGAACAAGA TGGATTGCAC
            121 GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG CTATTCGGCT ATGACTGGGC ACAACAGACA
            181 ATCGGCTGCT CTGATGCCGC CGTGTTCCGG CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT
            241 GTCAAGACCG ACCTGTCCGG TGCCCTGAAT GAACTGCAAG ACGAGGCAGC GCGGCTATCG
            301 TGGCTGGCCA CGACGGGCGT TCCTTGCGCA GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA
            361 AGGGACTGGC TGCTATTGGG CGAAGTGCCG GGGCAGGATC TCCTGTCATC TCACCTTGCT
            421 CCTGCCGAGA AAGTATCCAT CATGGCTGAT GCAATGCGGC GGCTGCATAC GCTTGATCCG
            481 GCTACCTGCC CATTCGACCA CCAAGCGAAA CATCGCATCG AGCGAGCACG TACTCGGATG
            541 GAAGCCGGTC TTGTCGATCA GGATGATCTG GACGAAGAGC ATCAGGGGCT CGCGCCAGCC
            601 GAACTGTTCG CCAGGCTCAA GGCGAGCATG CCCGACGGCG AGGATCTCGT CGTGACCCAT
            661 GGCGATGCCT GCTTGCCGAA TATCATGGTG GAAAATGGCC GCTTTTCTGG ATTCATCGAC
            721 TGTGGCCGGC TGGGTGTGGC GGACCGCTAT CAGGACATAN CGTTGGCTAC CCGTGATATT
            781 GCTGAANAGC TTGNNGGCGA ATGGNCTGAN CGCTTCCTCG TGCTTTACNN TATCGCCGCT
            841 CCCGANTCGC ANCGCATCGC CTTCTATCGC NNTCTTGANG ANTTCNTTCT GAGCGGGANN
            901 CTNGGGTNCN AAATGANCGA NNNANCGACN NCCNANNNNG NNNTCANNAG ATTTNNANTN
          961 NNNNGNCNGC CNTNCTNNNN AANNNNNNNN NN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591. [link to RRC of NBRP]

          2024.03.26

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