Please review the QC test results indicated by icon below as well as clone information before placing your order.

pMK409 (mAID-Nluc BSD)

A plasmid for construction of C-terminal mAID-Nluc BSD tagging donors.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution

In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp. Additional terms and conditions: The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.

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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019)。 学術研究にのみ限定、商業営利目的利用を禁ずる。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に相談すること。 付加的提供条件： 本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。
提供依頼	提供依頼書 [Word] 提供同意書 (MTA, 非営利学術目的用)[Word] 提供同意書 (MTA, 営利目的用)[Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

Reference section:

Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB16826_A8Lpp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pUC-M13_-100 () Sequence file: RDB16826_A8Lpa.seq
>D06157C4_A8Lp_1_pUC-M13_-100_E09_15_ABI24.ab1     1 GATGGTCAGG CGATTAGTTG GGTACGCCAG GGTTTTCCCA GTCACGACGT TGTAAAACGA    61 CGGCCAGTGA GCGCGCGTAA TACGACTCAC TATAGGGCGA ATTGGAGCTC CCCGGGATCC   121 GGTGCAGGCG CCAAGGAGAA GAGTGCTTGT CCTAAAGATC CAGCCAAACC TCCGGCCAAG   181 GCACAAGTTG TGGGATGGCC ACCGGTGAGA TCATACCGGA AGAACGTGAT GGTTTCCTGC   241 CAAAAATCAA GCGGTGGCCC GGAGGCGGCG GCGTTCGTGA AGGTATCAAT GGACGGAGCA   301 CCGTACTTGA GGAAAATCGA TTTGAGGATG TATAAAGCTA GCATGGTCTT CACACTCGAA   361 GATTTCGTTG GGGACTGGCG ACAGACAGCC GGCTACAACC TGGACCAAGT CCTTGAACAG   421 GGAGGTGTGT CCAGTTTGTT TCAGAATCTC GGGGTGTCCG TAACTCCGAT CCAAAGGATT   481 GTCCTGAGCG GTGAAAATGG GCTGAAGATC GACATCCATG TCATCATCCC GTATGAAGGT   541 CTGAGCGGCG ACCAAATGGG CCAGATCGAA AAAATTTTTA AGGTGGTGTA CCCTGTGGAT   601 GATCATCACT TTAAGGTGAT CCTGCACTAT GGCACACTGG TAATCGACGG GGTTACGCCG   661 AACATGATCG ACTATTTCGG ACGGCCGTAT GAAGGCATCG CCGTGTTCGA CGGCAAAAAG   721 ATCACTGTAA CAGGGACCCT GTGGAACGGC AACAAAATTA TCGACGAGCG CCTGATCAAC   781 CCCGACGGCT CCCTGCTGTT CCGAGTAACC ATCAACGGAG TGACCGGCTG GCGGCTGTGC   841 GAAACGCATT CTGGCGTAAG CGGCCGCTCG AGTCTAGATA ACTGATCATA ATCAGCCATA   901 CCACATTTGT AGAGGTTTTA CTTGCTTTAA AAAACCTCCC ACACCTCCCC CCTGAAACCT   961 GAAACATAAA ATGAAATGCA ATTGTTGTTG TTAACTTTGG TTTATTGCAG CCTTATTAAT  1021 TGGTTTACAA ATAAAGCCAA TTAGCATTCA CAAACTTTCA ACAAACTAAG CATTTTTTCT 1081 CACTGCCATT CCTTAAGTTT GTTTG //
Primer: reverse () Sequence file: RDB16826_A8Lpb.seq
>D06157C4_A8Lp_1_reverse_F09_18_ABI24.ab1     1 CAAAACATTA CCCTCACTAA AGGGAACAAA AGCTGGGTAC CGGATCCTAG TGAACCTCTT    61 CGAGGGACCT AATAACTTCG TATAGCATAC ATTATACGAA GTTATATTAA GGGTTCCGGA   121 TCTCGACCAG CTTCTGATGG AATTAGAACT TGGCAAAACA ATACTGAGAA TGAAGTGTAT   181 GTGGAACAGA GGCTGCTGAT CTCGTTCTTC AGGCTATGAA ACTGACACAT TTGGAAACCA   241 CAGTACTTAG AACCACAAAG TGGGAATCAA GAGAAAAACA ATGATCCCAC GAGAGATCTA   301 TAGATCTATA GATCATGAGT GGGAGGAATG AGCTGGCCCT TAATTTGGTT TTGCTTGTTT   361 AAATTATGAT ATCCAACTAT GAAACATTAT CATAAAGCAA TAGTAAAGAG CCTTCAGTAA   421 AGAGCAGGCA TTTATCTAAT CCCACCCCAC CCCCACCCCC GTAGCTCCAA TCCTTCCATT   481 CAAAATGTAG GTACTCTGTT CTCACCCTTC TTAACAAAGT ATGACAGGAA AAACTTCCAT   541 TTTAGTGGAC ATCTTTATTG TTTAATAGAT CATCAATTTC TGCATCCCGG GGATCTGATA   601 TCATCGATTA GCCCTCCCAC ACATAACCAG AGGGCAGCAA TTCACGAATC CCAACTGCCG   661 TCGGCTGTCC ATCACTGTCC TTCACTATGG CTTTGATCCC AGGATGCAGA TCGAGAAGCA   721 CCTGTCGGCA CCGTCCGCAG GGGCTCAAGA TGCCCCTGTT CTCATTTCCG ATCGCGACGA   781 TACAAGTCAG GTTGCCAGCT GCCGCAGCAG CAGCAGTGCC CAGCACCACG AGTTCTGCAC   841 AAGGTCCCCC CAGTAAAATG ATATACATTG ACACCAGTGA AGATGCGGCC GTCGCTAGAG   901 AGAGCTGCGC TGGCGACGCT GTAGTCTTCA GAGATGGGGG ATGCTGTTGA TTGTAGCCCG   961 TTGCTCTTTC AATGAGGTGG ATTCTTCTTT GAGACAAAGG CTTGGCCCAT GGTGGCTCGA  1021 GAATCTAGAT GATGGCAAGT CGAACGCCAG GAGATGAGGA GAAGGAGAAC AGCGCCGGCA 1081 GAACGGTGC //
Primer: PGK_N_rev () Sequence file: RDB16826_A8Lpc.seq
>D06157C4_A8Lp_1_PGK_N_rev_G09_21_ABI24.ab1     1 CCAGGGGCGG CCTCCCTACC CGGTAGATTT CGAGGTCGAG ATCCTAAGCT TGGCTGGACG    61 TAAACTCCTC TTCAGACCTA ATAACTTCGT ATAGCATACA TTATACGAAG TTATATTAAG   121 GGTTATTGAA TATGATCGAC GCGTTAAGAT ACATTGATGA GTTTGGACAA ACCACAACTA   181 GAATGCAGTG AAAAAAATGC TTTATTTGTG AAATTTGTGA TGCTATTGCT TTATTTGTAA   241 CCATTATAAG CTGCAATAAA CAAGTTAACA ACAACAATTG CATTCATTTT ATGTTTCAGG   301 TTCAGGGGGA GGTGTGGGAG GTTTTTTAAA GCAAGTAAAA CCTCTACAAA TGTGGTATGG   361 CTGATTATGA TCAGTTATCT AGACTCGAGC GGCCGCTTAC GCCAGAATGC GTTCGCACAG   421 CCGCCAGCCG GTCACTCCGT TGATGGTTAC TCGGAACAGC AGGGAGCCGT CGGGGTTGAT   481 CAGGCGCTCG TCGATAATTT TGTTGCCGTT CCACAGGGTC CCTGTTACAG TGATCTTTTT   541 GCCGTCGAAC ACGGCGATGC CTTCATACGG CCGTCCGAAA TAGTCGATCA TGTTCGGCGT   601 AACCCCGTCG ATTACCAGTG TGCCATAGTG CAGGATCACC TTAAAGTGAT GATCATCCAC   661 AGGGTACACC ACCTTAAAAA TTTTTTCGAT CTGGCCCATT TGGTCGCCGC TCAGACCTTC   721 ATACGGGATG ATGACATGGA TGTCGATCTT CAGCCCATTT TCACCGCTCA GGACAATCCT   781 TTGGATCGGA GTTACGGACA CCCCGAGATT CTGAAACAAA CTGGACACAC CTCCCTGTTC   841 AAGGACTTGG TCCAGGTTGT AGCCGGCTGT CTGTCGCCAG TCCCCAACGA AATCTTCGAG   901 TGTGAAGACC ATGCTAGCTT TATACATCCT CAAATCGATT TTCCTCAAGT ACGGTGCTCC   961 GTCCCATTGA TACCTTCACG AACGCCCGCC GCCTCCTGGC CACCGCTTTG ATTTTTGGCA  1021 GAAACAATCA CGTTCTTCCG GTATGGATCT CACCGGTGGT CATCCACACT GTGCCTTGAC 1081 TGAGTTGGGC CTGGGATC //
Primer: PGKpro_F () Sequence file: RDB16826_A8Lpd.seq
>D06157C4_A8Lp_1_PGKpro_F_H09_24_ABI24.ab1     1 AGTGGCGCCT ATCTCTCTTC TCATCTCCGG GCCTTTCGAC CTGCATCCAT CTAGATCTCG    61 AGCCACCATG GCCAAGCCTT TGTCTCAAGA AGAATCCACC CTCATTGAAA GAGCAACGGC   121 TACAATCAAC AGCATCCCCA TCTCTGAAGA CTACAGCGTC GCCAGCGCAG CTCTCTCTAG   181 CGACGGCCGC ATCTTCACTG GTGTCAATGT ATATCATTTT ACTGGGGGAC CTTGTGCAGA   241 ACTCGTGGTG CTGGGCACTG CTGCTGCTGC GGCAGCTGGC AACCTGACTT GTATCGTCGC   301 GATCGGAAAT GAGAACAGGG GCATCTTGAG CCCCTGCGGA CGGTGCCGAC AGGTGCTTCT   361 CGATCTGCAT CCTGGGATCA AAGCCATAGT GAAGGACAGT GATGGACAGC CGACGGCAGT   421 TGGGATTCGT GAATTGCTGC CCTCTGGTTA TGTGTGGGAG GGCTAATCGA TGATATCAGA   481 TCCCCGGGAT GCAGAAATTG ATGATCTATT AAACAATAAA GATGTCCACT AAAATGGAAG   541 TTTTTCCTGT CATACTTTGT TAAGAAGGGT GAGAACAGAG TACCTACATT TTGAATGGAA   601 GGATTGGAGC TACGGGGGTG GGGGTGGGGT GGGATTAGAT AAATGCCTGC TCTTTACTGA   661 AGGCTCTTTA CTATTGCTTT ATGATAATGT TTCATAGTTG GATATCATAA TTTAAACAAG 721 CAAAACCAAA TTAAGGGCCA GCTCATTCCT CCCACT //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.10.05

GNP_filter3_RDBDEP_html_231001.pl