Please review the QC test results indicated by icon below as well as clone information before placing your order.

pT2b_mT_gvpC16-T2A-EGFP_pP_rm

Tol2 transposon expression vector of Planktothrix rubescens gvpC16 and EGFP marker (C).

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Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

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In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Mizushima, R. et al., Contrast Media Mol. Imaging 2020: 5425934 (2020)) and an acknowledgment to the DEPOSITOR are requested. Additional terms and conditions: The Tol2 system is covered by patents owned by the National Institute of Genetics (NIG) Japan. For use of the Tol2 system except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by Dr. Koichi Kawakami of the National Institute of Genetics (Urasaki,A. et al., Genetics 174, 639-649 (2006); Kawakami, K. et al., Developmental Cell 7: 133-144 (2004))is requested.

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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献 (Mizushima, R. et al., Contrast Media Mol. Imaging 2020: 5425934 (2020)) を引用し、謝辞の表明を必要とする。 付加的提供条件： Tol2 systemは、国立遺伝学研究所が特許を保有している。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に連絡すること。利用者は、研究成果の公表にあたって国立遺伝学研究所 川上浩一博士が指定する文献 (Urasaki,A. et al., Genetics 174, 639-649 (2006); Kawakami, K. et al., Developmental Cell 7: 133-144 (2004)) の引用を必要とする。
提供依頼	提供依頼書 [Word] 提供同意書 (MTA, 非営利学術目的用)[Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB16904_A9Afp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: beta-gi_F (Pr0497) Region: gvpC16, T2A, EGFP 5' Sequence file: RDB16904_A9Afa.seq
>D05911A2_A9Af_2_beta-gi_F_A11_02_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNTTTNNNNC NGCTCCTGGG CAACGTGCTG GTTGTTGTGC    61 TGTCTCATCA TTTTGGCAAA GCTAGCATGG CCCTGAAGGA CAAATGGCAG CAGGATCGAA   121 TCGGCCGCCA GCAGGGAGTG CAGGAACGAC AGCAGCAGGT CCAGACCACA CTGTCTCTCT   181 GGCAGCAGGA GAGACAGAAC CAGGCTAGTG AGTTCCGGGA AGACCTGGAG TACAGGGTGA   241 GAGATCTGCT CGCAAATTAT CAGAAGCAGC ACCAGCAGAT GGCCGCTCAG CTGCAGCAGC   301 AGCTCCAGCA GAGCCGAACA GAAAGGAAGG ACGCAGTGCA GAAACTGTTC GAAGATCTCG   361 GCGTCTTTCG CGCCGAGCTG CAGGACTACC ATCTGAAGCT CCAGCAGACC GTGTGGGGAA   421 GCTCCCACCG ACATCCCATC AAGCCCCAGC CTACTGTGAA CCCAGGAGTC CCACAGCCAG   481 CTACCCTGGA TCAGCCCCAG GGTGAATTCG AGGGCAGAGG AAGTCTTCTA ACATGCGGTG   541 ACGTGGAGGA GAATCCCGGC CCTATGGTGA GCAAGGGCGA GGAGCTGTTC ACCGGGGTGG   601 TGCCCATCCT GGTCGAGCTG GACGGCGACG TAAACGGCCA CAAGTTCAGC GTGTCCGGCG   661 AGGGCGAGGG CGATGCCACC TACGGCAAGC TGACCCTGAA GTTCATCTGC ACCACCGGCA   721 AGCTGCCCGT GCCCTGGCCC ACCCTCGTGA CCACCCTGAC CTACGGCGTG CAGTGCTTCA   781 GCCGCTACCC CGACCACATG AAGCAGCACG ACTTCTTCNA GTCCGCCATG CCCGANNNTA   841 CNTCCAGGAN CGCACCATCT TCTTCAAGGA CGANNGCAAC TACAAGANCC GCGCCGAGGT   901 GAAGTTCGAG GGCGACACCC NGGNGAANCG CNTCNAGCTG ANNNNCATCN ACTTCNANNA   961 NNANNNNNNC ATCCTGGGGN NNNNGCTGGA NTACAACTAC NNNAGCNNNN NNGTCNATAT 1021 CATGNNNNAN NNNNNNNNN //
Primer: PGK_N_rev (Pr0243) Region: BGH pA_1, EGFP 3' Sequence file: RDB16904_A9Afb.seq
>D05911A2_A9Af_2_PGK_N_rev_E10_13_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNT TNNNCCTCNG ANNCCNTAGA GCCCNCCGCA TCCCCAGCAT    61 GCCTGCTATT GTCTTCCCAA TCCTCCCCCT TGCTGTCCTG CCCCACCCCA CCCCCCAGAA   121 TAGAATGACA CCTACTCAGA CAATGCGATG CAATTTCCTC ATTTTATTAG GAAAGGACAG   181 TGGGAGTGGC ACCTTCCAGG GTCAAGGAAG GCACGGGGGA GGGGCAAACA ACAGATGGCT   241 GGCAACTAGA AGGCACAGTC GAGGCTGATC AGCGAGCTCT AGATCATCGA TCAAGCTTAT   301 CGAGCGGCCG CTTACTTGTA CAGCTCGTCC ATGCCGAGAG TGATCCCGGC GGCGGTCACG   361 AACTCCAGCA GGACCATGTG ATCGCGCTTC TCGTTGGGGT CTTTGCTCAG GGCGGACTGG   421 GTGCTCAGGT AGTGGTTGTC GGGCAGCAGC ACGGGGCCGT CGCCGATGGG GGTGTTCTGC   481 TGGTAGTGGT CGGCGAGCTG CACGCTGCCG TCCTCGATGT TGTGGCGGAT CTTGAAGTTC   541 ACCTTGATGC CGTTCTTCTG CTTGTCGGCC ATGATATAGA CGTTGTGGCT GTTGTAGTTG   601 TACTCCAGCT TGTGCCCCAG GATGTTGCCG TCCTCCTTGA AGTCGATGCC CTTCAGCTCG   661 ATGCGGTTCA CCAGGGTGTC GCCCTCGAAC TTCACCTCGG CGCGGGTCTT GTAGTTGCCG   721 TCGTCCTTGA AGAAGANGGT GCGCTCCTGG ACGTAGCCTT CGGGCATGGC GGANTTGAAG   781 AANTCGTGCT GCTTCATGTG GTCNGGGTAG CGGCTGAAGC ACTGCACGCC GTNNNTCANG   841 GNGNTCACGA NGGTGGGCCA NGGCANGGGC AGCTTGNCGG NGGNGCANAN GAANTTCNNG   901 NTCAGCTTGN CGNNNNGGNA TCGCCNNCGC CNNCGCCGGA NACGCNNANC TTGTGNNNGT 961 TTACNTCNCC GNCCNNNNCN NNNNNNNNGG GCANNNCCCC NGNNNANNAN NNNNNNNNN //
Primer: PGKpro_F (Pr0249) Region: PuroR, BGH pA_2 Sequence file: RDB16904_A9Afc.seq
>D05911A2_A9Af_2_PGKpro_F_E01_05_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNTCCNG GCCTTTCGAC CTGNGGCCNC NACCNTGACC    61 GAGTACAAGC CCACGGTGCG CCTCGCCACC CGCGACGACG TCCCCAGGGC CGTACGCACC   121 CTCGCCGCCG CGTTCGCCGA CTACCCCGCC ACGCGCCACA CCGTCGATCC GGACCGCCAC   181 ATCGAGCGGG TCACCGAGCT GCAAGAACTC TTCCTCACGC GCGTCGGGCT CGACATCGGC   241 AAGGTGTGGG TCGCGGACGA CGGCGCCGCG GTGGCGGTCT GGACCACGCC GGAGAGCGTC   301 GAAGCGGGGG CGGTGTTCGC CGAGATCGGC CCGCGCATGG CCGAGTTGAG CGGTTCCCGG   361 CTGGCCGCGC AGCAACAGAT GGAAGGCCTC CTGGCGCCGC ACCGGCCCAA GGAGCCCGCG   421 TGGTTCCTGG CCACCGTCGG CGTCTCGCCC GACCACCAGG GCAAGGGTCT GGGCAGCGCC   481 GTCGTGCTCC CCGGAGTGGA GGCGGCCGAG CGCGCCGGGG TGCCCGCCTT CCTGGAGACC   541 TCCGCGCCCC GCAACCTCCC CTTCTACGAG CGGCTCGGCT TCACCGTCAC CGCCGACGTC   601 GAGGTGCCCG AAGGACCGCG CACCTGGTGC ATGACCCGCA AGCCCGGTGC CTGACGCCCG   661 CCCCACGACC CGCAGCGCCC GACCGAAAGG AGCGCACGAC CCCATGCATC GATGATCTAG   721 AGCTCGCTGA TCAGCCTCGA CTGTGCCTTC TAGTTGCCAG CCATCTGTTG TTTGCNCCTC   781 CCCCGTGCCT TCCTTGANCC TGGAANGTGC CACTCCCACT GTCCTTTCCT AATAAAATGA   841 GGAAATTGCA TCGCATTGTC TGANTANGNG TCATTCTATT CTGGGGGGGN GGGGGNGGGG   901 CANGANAGCA NNGGGGGANG ANTGGGAAGA NNNTANNNNN NNTGCTNGGG GNTGCGGNGG 961 GCTCTATNGN NNCANCCNNN NTTNNNNNAA NNNNNNNNNN //
Primer: f1ori_R3 (Pr0142) Region: Left Tol2, I-SceI, cHS4 ins_1 Sequence file: RDB16904_A9Afd.seq
>D05911A2_A9Af_2_f1ori_R3_F11_17_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNC NCANNGTTGG GANGGCGANC GGTGCGGGCC TCTTCGCTAT    61 TACGCCAGCT GGCGAAAGGG GGATGTGCTG CAAGGCGATT AAGTTGGGTA ACGCCAGGGT   121 TTTCCCAGTC ACGACGTTGT AAAACGACGG CCAGTGAATT GTAATACGAC TCACTATAGG   181 GCGAATTGGG TACCAGAGGT GTAAAGTACT TGAGTAATTT TACTTGATTA CTGTACTTAA   241 GTATTATTTT TGGGGATTTT TACTTTACTT GAGTACAATT AAAAATCAAT ACTTTTACTT   301 TTACTTAATT ACATTTTTTT AGAAAAAAAA GTACTTTTTA CTCCTTACAA TTTTATTTAC   361 AGTCAAAAAG TACTTATTTT TTGGAGATCA CTTGTCGAGT AGGGATAACA GGGTAATGAT   421 CCCCCGGGCT GCAGTCGCGA CGCGTCCTAG AGGGACAGCC CCCCCCCAAA GCCCCCAGGG   481 ATGTAATTAC GTCCCTCCCC CGCTAGGGGG CAGCAGCGAG CCGCCCGGGG CTCCGCTCCG   541 GTCCGGCGCT CCCCCCGCAT CCCCGAGCCG GCAGCGTGCG GGGACAGCCC GGGCACGGGG   601 AAGGTGGCAC GGGATCGCTT TCCTCTGAAC GCTTCTCGCT GCTCTTTGAG CCTGCAGACA   661 CCTGGGGGGA TACGGGGAAA AAGCTTTAGG CTGAAAGAGA GATTTAGAAT GACAGAATCA   721 TAGAACGGCC TGGGTTGCAA AGGAGCACAG TGCTCATCCA GATCCNACCC CCTGCTATGT   781 GCANGGTCAT CAACCANCNN NNCAGGCTGC NCNNAGCNNC ATCCAGCCTG GCCTTGAATG   841 CCTGCANGGA TGGGGCATCC ACAGCCTCCT TGGGCAACCN GTTNNGTGCG TCACCNCCCT   901 CTGGGGGAAA AACTGCCNCC TCANATCNNN NCCNAANCTN CCCNGNCNCN NTGNNAAGCC   961 NTTNCCCCNN GNNNNNTNNN NGGGGANTTT NNTGNGANAT NGNNGGNCTG GGGNNNNNNA 1021 NNNNNNNNNA NNNNNNGCNT CNNNNNNNNN N //
Primer: beta-gro_rev (Pr0512) Region: intron, mini CMV pro, tet RE, pA, cHS4 ins_1 Sequence file: RDB16904_A9Afe.seq
>D05911A2_A9Af_2_beta-gro_rev_C11_08_ABI24.ab1     1 NNNNNNNNNN NNNNNNANTT TGCCCCNTCN NTATAACATG AATTTTACAA TAGCGAAAAA    61 GAAAGAACAA TCAAGGGTCC CCAAACTCAC CCTGAAGTTC TCAAGGCTGG ATCGGTCCCG   121 GTGTCTTCTA TGGAGGTCAA AACAGCGTGG ATGGCGTCTC CAGGCGATCT GACGGTTCAC   181 TAAACGAGCT CTGCTTATAT AGGCCTCCCA CCGTACACGC CTACCTCGAC CCGGGTACCG   241 AGCTCGACTT TCACTTTTCT CTATCACTGA TAGGGAGTGG TAAACTCGAC TTTCACTTTT   301 CTCTATCACT GATAGGGAGT GGTAAACTCG ACTTTCACTT TTCTCTATCA CTGATAGGGA   361 GTGGTAAACT CGACTTTCAC TTTTCTCTAT CACTGATAGG GAGTGGTAAA CTCGACTTTC   421 ACTTTTCTCT ATCACTGATA GGGAGTGGTA AACTCGACTT TCACTTTTCT CTATCACTGA   481 TAGGGAGTGG TAAACTCGAC TTTCACTTTT CTCTATCACT GATAGGGAGT GGTAAACTCG   541 ATGTAAGCTT GCCGTTGTCG ACCACACAAA AAACCAACAC ACAGATGTAA TGAAAATAAA   601 GATATTTTAT TCCTAGAGCC CCATCCTCAC TGACTCCGTC CTGGAGTTGG ATGAGAGATA   661 ATGGCCTTAC GTTGTGCCAG GGGAGGGTCG GGCTGGATTT AGCAAGATTT ACCTTCTCCA   721 AAGAGCGGTG CTGCAGTGGC ACAGCTGCCC ACGGANGTGG GGGGGTCACC GTCCCTGGAN   781 GTGANGAAGA ACTGTGGGGA NGTGGCACTG ANGGNNATGG CCAGTGGGCA CGGTGGGTGG   841 GTTGGGGTTG GTCTTNNGGA TCTTGGANGG NTTTTCCAGC CTTCATGATT TGNCNANTGT   901 ATGANCNTCT ACNTGGNAAT TCTCCANCTG CCNGNNCCAN NCCTACNGAN CCNGCNNTAT   961 NTNNNNNGGN NNGCTCNNNN NNCCNTTNTG NTNNCNTNCN NANNNNNTCA ANNNTGCNNN 1021 NCNNANNNNN NNNNANNNNN NNNNN //
Primer: tetR-C_F2 (Pr0862) Region: tetR, SV40 NLS, KRAB, BGH pA_2 Sequence file: RDB16904_A9Aff.seq
>D05911A2_A9Af_2_tetR-C_F2_F01_06_ABI08.ab1     1 NNNNNNNNNN NNNNNNCNNN NNGNNCTTCN NTTTCGGGCT GAANTCATTA ATATTTGGAT    61 TTTCTGCACA GTTAGATGAA AAAAAGCATA CACCCATTGA GGATGGTAAT AAATCGCCAA   121 AAAAGAAGAG AAAGGTCGAT GGCGGTGGTG CTTTGTCTCC TCAGCACTCT GCTGTCACTC   181 AAGGAAGTAT CATCAAGAAC AAGGAGGGCA TGGATGCTAA GTCACTAACT GCCTGGTCCC   241 GGACACTGGT GACCTTCAAG GATGTATTTG TGGACTTCAC CAGGGAGGAG TGGAAGCTGC   301 TGGACACTGC TCAGCAGATC GTGTACAGAA ATGTGATGCT GGAGAACTAT AAGAACCTGG   361 TTTCCTTGGG TTATCAGCTT ACTAAGCCAG ATGTGATCCT CCGGTTGGAG AAGGGAGAAG   421 AGCCCTGGCT GGTGGAGAGA GAAATTCACC AAGAGACCCA TCCTGATTCA GAGACTGCAT   481 TTGAAATCAA ATCATCAGTT TAAGGATCCG AGCTCGGTGA TCCGCTGGCA CGACAGGTTT   541 CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATG TGAGTTAGCT CACTCATTAG   601 GCACCCCAGG CTTTACACTT TATGCTTCCG GCTCGTATGT TGTGTGGAAT TGTGAGCGGA   661 TAACAATTTC ACACAGGAAA CAGCTATGAC CATGATTACG CCAAGCTTGG GCTGCAGCCA   721 TAGAGCCCAC CGCATCCCCA GCATGCCTGC TATTGTCTTC NCAATCCTCC CCCTTGCTGT   781 CCTGCCCCAC CCCACCCCCC ANAATAGAAT GACACCTACT CAGACAATGC GATGCNNNTN   841 CCTCATTTTA TTNNNAAAGG ANAGTGGGAN NNNCACNTTC CNNGGTCAAG GNNNGNNCNG   901 GGGGANGGGC NNNNNNCAGA NNGNNNNCAA CTANAANGNN NNANNCNNNN CTNNTCANCN 961 ANCNNNANNT CATCNNNNCN NNGNGNNNNN NNNNN //
Primer: IRES-F (Pr0036) Region: IRES, tetR, SV40NLS Sequence file: RDB16904_A9Afg.seq
>D05911A2_A9Af_2_IRES-F_E05_14_ABI24.ab1     1 NNNNNNNNNN NNNNNTTNNG TCNAGGNNNN NNAACGTCTA GGCCCCCCGA ACCACGGGGN    61 CGTGGTTTTC CTTTGAAAAA CACGATGATA ATATGGCTAG ATTAGATAAA AGTAAAGTGA   121 TTAACAGCGC ATTAGAGCTG CTTAATGAGG TCGGAATCGA AGGTTTAACA ACCCGTAAAC   181 TCGCCCAGAA GCTTGGTGTA GAGCAGCCTA CATTGTATTG GCATGTAAAA AATAAGCGGG   241 CCCTTATGAA CATGCTTTCA GAGGCAATAC TGGCGAAGCA TCACACCCGT TCAGCACCGT   301 TACCGACTGA GAGTTGGCAG CAGTTTCTCC AGGAAAATGC TCTGAGTTTC CGTAAAGCAT   361 TACTGGTCCA TCGTGATGGA GCCCGATTGC ATATAGGGAC CTCTCCTACG CCCCCCCAGT   421 TTGAACAAGC AGAGGCGCAA CTACGCTGTC TATGCGATGC AGGGTTTTCG GTCGAGGAGG   481 CTCTTTTCAT TCTGCAATCT ATCAGCCATT TTACGTTGGG TGCAGTATTA GAGGAGCAAG   541 CAACAAACCA GATAGAAAAT AATCATGTGA TAGACGCTGC ACCACCATTA TTACAAGAGG   601 CATTTAATAT TCAGGCGAGA ACCTCTGCTG AAATGGCCTT CCATTTCGGG CTGAAATCAT   661 TAATATTTGG ATTTTCTGCA CAGTTAGATG AAAAAAAGCA TACACCCATT GAGGATGGTA   721 ATAAATCGCC NAAAAAGAAG AGAAAGGNCG ATGGCGGTGG TGCTTTGTCT CCTCAGCACT   781 CTGCTGTCAC TCNAGGAAGT ATCATCAAGA ACAAGGANGG CATGGATGCT AAGTCACTAA   841 CTGCCTGGTC CCGGACACTG GTGACCTTCN AGGANGTATT TGTGGANTTC ACCNNGGNNG   901 ANTGGAAGCT GCTGGNNNCN GCTCANCANA TCGNGTACNG AAATGNGANG CTGGAGAACT   961 ATNNNNNNNT GGTTTNNTNN GGTTANCANC TTNNTNNNCC NNANNNGNNN CNNCNNGNNN 1021 GNNANNNNNN NNNN //
Primer: SV40pro_F (Pr0110) Region: SV40 pro_ori, rtTA-Advenced Sequence file: RDB16904_A9Afh.seq
>D05911A2_A9Af_2_SV40pro_F_H10_22_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNTGNNGN NGNTTTTTTG GANGCCTAGG CTTTTGCAAA    61 AAGCTCTCTG GCTAACTAGA GAACCCACTG CTTACTGGCT TATCGAAATT AATACGACTC   121 ACTATAGGGA GACCCAAGCT GCAATTCACC ATGAGCAGAC TGGACAAGAG CAAAGTCATA   181 AACGGCGCTC TGGAATTACT CAATGGAGTC GGTATCGAAG GCCTGACGAC AAGGAAACTC   241 GCTCAAAAGC TGGGAGTTGA GCAGCCTACC CTGTACTGGC ACGTGAAGAA CAAGCGGGCC   301 CTGCTCGATG CCCTGCCAAT CGAGATGCTG GACAGGCATC ATACCCACTT CTGCCCCCTG   361 GAAGGCGAGT CATGGCAAGA CTTTCTGCGG AACAACGCCA AGTCATTCCG CTGTGCTCTC   421 CTCTCACATC GCGACGGGGC TAAAGTGCAT CTCGGCACCC GCCCAACAGA GAAACAGTAC   481 GAAACCCTGG AAAATCAGCT CGCGTTCCTG TGTCAGCAAG GCTTCTCCCT GGAGAACGCA   541 CTGTACGCTC TGTCCGCCGT GGGCCACTTT ACACTGGGCT GCGTATTGGA GGAACAGGAG   601 CATCAAGTAG CAAAAGAGGA AAGAGAGACA CCTACCACCG ATTCTATGCC CCCACTTCTG   661 AGACAAGCAA TTGAGCTGTT CGACCGGCAG GGAGCCGAAN CTGCCTTCCT TTTCGGCCTG   721 GAACTAATCA TATGTGGCCT GGANAAACAG CTAAAGTGCG AAAGCGGCGG GCCGGCCGAC   781 GCCCTTGACN ANTTTGACTT ANACATGCTC NNNGCCGANG CCCTTGACGA NTTTGACCTT   841 GATATGCTGC CTGCTGANGC TCTTGACGAT TTTGANCTTG ACNTGCTCCC CGGGTAACTA   901 AGNNANGNAN CATCCNCCCC CCCCCCNNNA NGNNACTNGN NNNANNCCGC TNNNNNTNNN 961 NNCNGNNGNG CGTTNGNCNA TNNGNNATTT TCNNNCNTNN NNNNNNNN //
Primer: SV40pA3'Element reverse (Pr0108) Region: Right Tol2, I-SceI, cHS4 ins_2 Sequence file: RDB16904_A9Afi.seq
>D05911A2_A9Af_2_SV40pA3'element Reverse_G11_20_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNA ANGCNNGCAA CGCGGCCTTT TTACGGTTCC    61 TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG   121 ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC   181 GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCCAAT ACGCAAACCG CCTCTCCCCG   241 CGCGTTGGCC GATTCATTAA TGCAGCTGGC ACGACAGGTT TCCCGACTGG AAAGCGGGCA   301 GTGAGCGCAA CGCAATTAAT GTGAGTTAGC TCACTCATTA GGCACCCCAG GCTTTACACT   361 TTATGCTTCC GGCTCGTATG TTGTGTGGAA TTGTGAGCGG ATAACAATTT CACACAGGAA   421 ACAGCTATGA CCATGATTAC GCCAAGCTCG AAATTAACCC TCACTAAAGG GAACAAAAGC   481 TGGAGCTCCA GAGGTGTAAA AAGTACTCAA AAATTTTACT CAAGTGAAAG TACAAGTACT   541 TAGGGAAAAT TTTACTCAAT TAAAAGTAAA AGTATCTGGC TAGAATCTTA CTTGAGTAAA   601 AGTAAAAAAG TACTCCATTA AAATTGTACT TGAGTATTGG ATCAATTACC CTGTTATCCC   661 TATCGAGACG CGTCGGTCCG GCTAGAGCCC CATCCTCACT GACTCCGTCC TGGAGTTGGA   721 TGAGAGATAA TGGCCTTACG TTGTGCCANG GGANGGTCGG GCTGGATTTA GCAAGATTTA   781 NCTTCTCCAA AGAGCGGTGC TGCAGTGGCA CAGCTGCCNN CGGANGTGGG GGGGTCACCG   841 TCNCTGGANG TGATGANNAA CTGTGGGGAT GTGGCACTGA NGGANATGGC CNGTGGGCAC   901 NGNGGGTGGG TTGGGGTTGG NCTNGGGGNN CTTGGAGGGC TTTTCCANNN TNCANNANTT   961 GANNANNNTA TNNANNTCTA CATGGNNNNN NTNCANNNGN CNNGTNCNNN NNNTACNNNN 1021 NCNNNNNNNN TNNCNNNNNN NNGNNNNNNN NNN //
Primer: SV40pro_R (Pr0470) Region: SV40pro_ori, cHS4 ins_2 Sequence file: RDB16904_A9Afj.seq
>D05911A2_A9Af_2_SV40pro_R_B04_02_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNCNNC NNTGGNNNNN NNNANGGGCG GAACTGGGCG    61 GAGTTAGGGG CGGGNNGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT   121 GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG   181 ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC   241 ACCCTAACTG ACACACATTC CACAGCCGTT AATTAAGATC CCTAGAGGGA CAGCCCCCCC   301 CCAAAGCCCC CAGGGATGTA ATTACGTCCC TCCCCCGCTA GGGGGCAGCA GCGAGCCGCC   361 CGGGGCTCCG CTCCGGTCCG GCGCTCCCCC CGCATCCCCG AGCCGGCAGC GTGCGGGGAC   421 AGCCCGGGCA CGGGGAAGGT GGCACGGGAT CGCTTTCCTC TGAACGCTTC TCGCTGCTCT   481 TTGAGCCTGC AGACACCTGG GGGGATACGG GGAAAAAGCT TTAGGCTGAA AGAGAGATTT   541 AGAATGACAG AATCATAGAA CGGCCTGGGT TGCAAAGGAG CACAGTGCTC ATCCAGATCC   601 AACCCCCTGC TATGTGCAGG GTCATCAACC AGCAGCCCAG GCTGCCCAGA GCCACATCCA   661 GCCTGGCCTT GAATGCCTGC AGGGATGGGG CATCCACAGC CTCCTTGGGC AACCTGTTCA   721 GTGCGTCACC ACCCTCTGGG GGAAAAACTG CCTCCTCATA TCCAACCCAA ACCTCCCCTG   781 TCTCAGTGTA AAGCCATTCC CCCTTGNCCT ATCNANGGGG GGAGTTTGCT GTGACATTGT   841 NNGTCTGGGN GTGANNCATG NTNGNNNANN TTCANTGNNN TCACGGNNNN GCNNNNTNNT 901 TGGGGGATAN NGNAAGNNGC ANGNNNANNN NTGGNNNNNG NNGNNCNNNN NNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2024.03.26

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