RDB16905 - pT2b_mT_gvpC20-T2A-mKO2_pP

Tol2 transposon expression vector of Planktothrix rubescens gvpC20 and mKO2 marker (C).

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Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Mizushima, R. et al., Contrast Media Mol. Imaging 2020: 5425934 (2020)) and an acknowledgment to the DEPOSITOR are requested. Additional terms and conditions: The Tol2 system is covered by patents owned by the National Institute of Genetics (NIG) Japan. For use of the Tol2 system except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by Dr. Koichi Kawakami of the National Institute of Genetics (Urasaki,A. et al., Genetics 174, 639-649 (2006); Kawakami, K. et al., Developmental Cell 7: 133-144 (2004))is requested. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. The RECIPIENT who belongs to commercial organization should obtain written approval from the Medical & Biological Laboratories Co., Ltd prior to MTA.
Ordering	Order form [Credit Card Payment] [Bank Transfer Payment] MTA, for use for not-for-profit academic purpose [Word] Please visit Information of Request for Distribution.[link]

Terms and conditions for distribution

In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Mizushima, R. et al., Contrast Media Mol. Imaging 2020: 5425934 (2020)) and an acknowledgment to the DEPOSITOR are requested.
Additional terms and conditions:
The Tol2 system is covered by patents owned by the National Institute of Genetics (NIG) Japan. For use of the Tol2 system except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by Dr. Koichi Kawakami of the National Institute of Genetics (Urasaki,A. et al., Genetics 174, 639-649 (2006); Kawakami, K. et al., Developmental Cell 7: 133-144 (2004))is requested. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. The RECIPIENT who belongs to commercial organization should obtain written approval from the Medical & Biological Laboratories Co., Ltd prior to MTA.

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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献 (Mizushima, R. et al., Contrast Media Mol. Imaging 2020: 5425934 (2020)) を引用し、謝辞の表明を必要とする。付加的提供条件： Tol2 systemは、国立遺伝学研究所が特許を保有している。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に連絡すること。利用者は、研究成果の公表にあたって国立遺伝学研究所川上浩一博士が指定する文献 (Urasaki,A. et al., Genetics 174, 639-649 (2006); Kawakami, K. et al., Developmental Cell 7: 133-144 (2004)) の引用を必要とする。非営利機関での学術研究目的での利用に限る。営利機関の利用者もしくは商業利用の場合はMBL社から事前にその旨の提供承諾を取得する。
提供依頼	提供依頼書 [Word] 提供同意書 (MTA, 非営利学術目的用)[Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。

提供条件

利用者は、研究成果の公表にあたって寄託者の指定する文献 (Mizushima, R. et al., Contrast Media Mol. Imaging 2020: 5425934 (2020)) を引用し、謝辞の表明を必要とする。
付加的提供条件：
Tol2 systemは、国立遺伝学研究所が特許を保有している。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に連絡すること。利用者は、研究成果の公表にあたって国立遺伝学研究所川上浩一博士が指定する文献 (Urasaki,A. et al., Genetics 174, 639-649 (2006); Kawakami, K. et al., Developmental Cell 7: 133-144 (2004)) の引用を必要とする。非営利機関での学術研究目的での利用に限る。営利機関の利用者もしくは商業利用の場合はMBL社から事前にその旨の提供承諾を取得する。

提供依頼

提供依頼書 [Word

]
提供同意書 (MTA, 非営利学術目的用)[Word

]
手続きの概要は、「提供申込みについて[link]」をご覧ください。

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QC test results

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB16905	pT2b_mT_gvpC20-T2A-mKO2_pP_rm	DNA solution

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check

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Primer: beta-gi_F (Pr0497) Region: gvpC20, T2A, mKO2 5' Sequence file: RDB16905_A9Afa.seq
>D05911A3_A9Af_6_beta-gi_F_A12_03_ABI24.ab1 1 NNNNNNNNNN NNCNNNNTNN NCTTTCNNAN NGCTCCTGGG CAACGTGCTG GTTGTTGTGC 61 TGTCTCATCA TTTTGGCAAA GCTAGCATGG CTCTGAAGGA CAAATGGCAG CAGGATAGGA 121 TCGGCAGACA GCAGGGAGTG CAGGAACGGC AGCAGCAGGT CCAGACCACA CTGTCTCTCT 181 GGCAGCAGGA GAGGCAGAAC CAAGCAAGTG AGTTCCGCGA AGACCTGGAG TACCGAGTGA 241 CCGATCTGCT CGCAAATTAT CAGAAGCAGC GACTGGAAGC CAGGGAGACA CTGCTCGAAG 301 ACCTCGCTAT CTTTAGACAG ACTCTGTACC GGGAAGTCGA GGAATATCTG GGCGAGCTCG 361 ATATTCTGCA CCAGCAGATG GCCGCTCAGC TCCAGCAGCA GCTGCAGCAG AGCCGGACCG 421 AGCGAAAGGA CGCAGTGCAG AAACTCTTCG AAGATCTGGG CGTCTTTAGG GCCGAGCTGC 481 AGGACTACCA TCTGAAGCTC CAGCAGACAG TGTGGGGAAG CTCCCACAGA CATAGCATCA 541 AGCCCCAGCC TACTGTGAAC CCTGGAGTCC CACAGCCAGC TACCCTGGAT CAGCCACAGG 601 GTGAATTCGA GGGCAGAGGA AGTCTTCTAA CATGCGGTGA CGTGGAGGAG AATCCCGGCC 661 CTATGGTGAG CGTGATCAAG CCCGAGATGA AGATGAGGTA CTACATGGAC GGCTCCGTCA 721 ATGGGCATGA GTTCACAATC GAGGGTGANG GCACAGGCAG ACCTTACGAG GGACATCAGG 781 AGATGACACT GCGCGTCACA ATGGCCGANG GCGGGCCAAT GCCTTTCGCC TTCNACCTGG 841 TGTCCCACGN NTTCTGTTAC NGNCACNGAG TTTTTACTAA ATATCCNGAA NANATNCCAG 901 ANNATTTCNN GCNNGNCNTT CCTGAGGGNC NGNCCTGGGA NANGNCCCNG GNNTTNNANG 961 NNNNNGCTNN NNNNCCNNNN NCNNNNNNNT CNNCNNGNNG GNNNNCNNCN TNNNNNNNNN 1021 N //
Primer: PGK_N_rev (Pr0243) Region: BGH pA_1, mKO2 3' Sequence file: RDB16905_A9Afb.seq
>D05911A3_A9Af_6_PGK_N_rev_A11_02_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNCNCNNAAG CCNTAGAGCC CACCGCATCC CCAGCATGCC 61 TGCTATTGTC TTCCCNATCC TCCCCCTTGC TGTCCTGCCC CACCCCACCC CCCAGAATAG 121 AATGACACCT ACTCAGACAA TGCGATGCAA TTTCCTCATT TTATTAGGAA AGGACAGTGG 181 GAGTGGCACC TTCCAGGGTC AAGGAAGGCA CGGGGGAGGG GCAAACAACA GATGGCTGGC 241 AACTAGAAGG CACAGTCGAG GCTGATCAGC GAGCTCTAGA TCATCGATCA AGCTTATCGA 301 GCGGCCGCTT AGGAGTGGGC CACGGCGTCC TCCACCTGCT CGGTGATGTT GCCCTCGGTC 361 TTCCTCACCA GCCTGTGGCC GATGTAGTGG TCGCCGGGCA TCTCCAGGAT CTCCTTGGCG 421 GCCTTGTAGG TGGTCTTCAT CTGGCACTTG TGGTTGCCGC CGCCCTCCAG CTTCAGGTAC 481 ATGGTCACGT CGCCCTTCAG CACGCCGTCG CTGGCGGTGA TCTTCTCGGT GGAGGGCTCC 541 CAGTCCACGC TCTGGTTCTG CATGATGGGG CCGTCGGCGG GGAAGTTCAC GCCGGTGAAC 601 TTGGACTTGT GGTAGAAGGT GTTGCCCCTC AGGCTGATGT GGGCGCTCAC GGAGGCGGAG 661 CCGCCGTCCT CGAACTCCAG GGACCTCTCC CAGGACAGGC CCTCAGGAAA GGNCTGCTTG 721 AAATAGTCTG GGATCTCTTC TGGATATTTA GTAAAAACTC TGTGGCCGTA ACAGAACACG 781 TGGGANACCA GGTCGAANGN GAAAGGNATT GGCCCGCCCT CGGCCATTGT GACGCGCANT 841 GTCNTCTCCT GATGTCCCTC GTAANGNCTG CCNGNGCCCT CACCCTCNAN TGNGAACTCA 901 TGNCCATTGA CNGANCCNTC CNNGTANTAN CNCATCTTCA TCTCGGGCTN GATCACNCTC 961 ANCNTNNNNC NNGNNNTNNN NNNNNCNNCN NNNNNNNNN //
Primer: PGKpro_F (Pr0249) Region: PuroR, BGH pA_2 Sequence file: RDB16905_A9Afc.seq
>D05911A3_A9Af_6_PGKpro_F_B11_05_ABI24.ab1 1 NNNNNNNNNN NNNNNNNTNN NNNTCTCCGG GCCTTTCGAC CTGTGGCCNC AACCNTGACC 61 GAGTACAAGC CCACGGTGCG CCTCGCCACC CGCGACGACG TCCCCAGGGC CGTACGCACC 121 CTCGCCGCCG CGTTCGCCGA CTACCCCGCC ACGCGCCACA CCGTCGATCC GGACCGCCAC 181 ATCGAGCGGG TCACCGAGCT GCAAGAACTC TTCCTCACGC GCGTCGGGCT CGACATCGGC 241 AAGGTGTGGG TCGCGGACGA CGGCGCCGCG GTGGCGGTCT GGACCACGCC GGAGAGCGTC 301 GAAGCGGGGG CGGTGTTCGC CGAGATCGGC CCGCGCATGG CCGAGTTGAG CGGTTCCCGG 361 CTGGCCGCGC AGCAACAGAT GGAAGGCCTC CTGGCGCCGC ACCGGCCCAA GGAGCCCGCG 421 TGGTTCCTGG CCACCGTCGG CGTCTCGCCC GACCACCAGG GCAAGGGTCT GGGCAGCGCC 481 GTCGTGCTCC CCGGAGTGGA GGCGGCCGAG CGCGCCGGGG TGCCCGCCTT CCTGGAGACC 541 TCCGCGCCCC GCAACCTCCC CTTCTACGAG CGGCTCGGCT TCACCGTCAC CGCCGACGTC 601 GAGGTGCCCG AAGGACCGCG CACCTGGTGC ATGACCCGCA AGCCCGGTGC CTGACGCCCG 661 CCCCACGACC CGCAGCGCCC GACCGAAAGG AGCGCACGAC CCCATGCATC GATGATCTAG 721 AGCTCGCTGA TCAGCCTCGA CTGTGCCTTC TAGTTGCCAG CCATCTGTTG TTTGCCCCTC 781 CCCCGTGCCT TCCTTGACCC TGGAANGTGC CACTCCCACT GTCCTTTCCT AATAAAATGA 841 GGAAATTGCA TCGCATTGTC TGANTANGTG TCATTCTATT CTGGGGGGTG GGGTNNNNAN 901 GNNAGCAAGG GGGANGANTG GGAAGANAAT ANCNNNNTGC TGGGGNNGNN GNGGGCNCNA 961 TGGCNGCNNC CNNNNCTTNN NNNNNNCNTN GNNCATANCT GTTTNCNNGN NNNNNNNNN //
Primer: f1ori_R3 (Pr0142) Region: Left Tol2, I-SceI, cHS4 ins_1 Sequence file: RDB16905_A9Afd.seq
>D05911A3_A9Af_6_f1ori_R3_F12_18_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNCNNTTGG GANGGCGATC GGTGCGGGCC TCTTCGCTAT 61 TACGCCAGCT GGCGAAAGGG GGATGTGCTG CAAGGCGATT AAGTTGGGTA ACGCCAGGGT 121 TTTCCCAGTC ACGACGTTGT AAAACGACGG CCAGTGAATT GTAATACGAC TCACTATAGG 181 GCGAATTGGG TACCAGAGGT GTAAAGTACT TGAGTAATTT TACTTGATTA CTGTACTTAA 241 GTATTATTTT TGGGGATTTT TACTTTACTT GAGTACAATT AAAAATCAAT ACTTTTACTT 301 TTACTTAATT ACATTTTTTT AGAAAAAAAA GTACTTTTTA CTCCTTACAA TTTTATTTAC 361 AGTCAAAAAG TACTTATTTT TTGGAGATCA CTTGTCGAGT AGGGATAACA GGGTAATGAT 421 CCCCCGGGCT GCAGTCGCGA CGCGTCCTAG AGGGACAGCC CCCCCCCAAA GCCCCCAGGG 481 ATGTAATTAC GTCCCTCCCC CGCTAGGGGG CAGCAGCGAG CCGCCCGGGG CTCCGCTCCG 541 GTCCGGCGCT CCCCCCGCAT CCCCGAGCCG GCAGCGTGCG GGGACAGCCC GGGCACGGGG 601 AAGGTGGCAC GGGATCGCTT TCCTCTGAAC GCTTCTCGCT GCTCTTTGAG CCTGCAGACA 661 CCTGGGGGGA TACGGGGAAA AAGCTTTAGG CTGAAAGAGA GATTTAGAAT GACAGAATCA 721 TAGAACGGCC TGGGTTGCAA AGGAGCACAG TGCTCATCCA GATCCAACCC CCTGCTATGT 781 GCANGGTCAT CAACCAGCAG CCCAGGCTGC CNNNNNCCAC ATCCNNNCTG NCCTTGAATG 841 CCTGCAGGGA NGGGGCATCC NCAGCCTCCT NNGNCAACCT GNNCAGTGNG TCANCACCCT 901 CTGGGGGAAA AACTGCCNNC NCNTATCNNA NCCAANNNTC CCCTGTCTCN NNGNAAAGNC 961 ATTCCCCCTT GNNCTATNNN GGGGGANTTT GNTGTGNNNT TNNNGGNCTG GGGNNNNNCA 1021 TNTTTNNCNA NNNNNNNNNA TNNNNNNNNN N //
Primer: beta-gro_rev (Pr0512) Region: mini CMV pro, tet RE, pA, cHS4 ins_1 Sequence file: RDB16905_A9Afe.seq
>D05911A3_A9Af_6_beta-gro_rev_C12_09_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNANNNNT TNNNNNNNNN NATAANNTGA ATTTTACAAT 61 AGCGAAAAAG AAAGAACAAT CAAGGGTCCC CAAANTCNNN CTGAAGTTCT CAAGGCTGGA 121 TCGGTCCCGG TGTCTTCTAT GGAGGTCAAA ACAGCGTGGA TGGCGTCTCC AGGCGATCTG 181 ACGGTTCACT AAACGAGCTC TGCTTATATA GGCCTCCCAC CGTACACGCC TACCTCGACC 241 CGGGTACCGA GCTCGACTTT CACTTTTCTC TATCACTGAT AGGGAGTGGT AAACTCGACT 301 TTCACTTTTC TCTATCACTG ATAGGGAGTG GTAAACTCGA CTTTCACTTT TCTCTATCAC 361 TGATAGGGAG TGGTAAACTC GACTTTCACT TTTCTCTATC ACTGATAGGG AGTGGTAAAC 421 TCGACTTTCA CTTTTCTCTA TCACTGATAG GGAGTGGTAA ACTCGACTTT CACTTTTCTC 481 TATCACTGAT AGGGAGTGGT AAACTCGACT TTCACTTTTC TCTATCACTG ATAGGGAGTG 541 GTAAACTCGA TGTAAGCTTG CCGTTGTCGA CCACACAAAA AACCAACACA CAGATGTAAT 601 GAAAATAAAG ATATTTTATT CCTAGAGCCC CATCCTCACT GACTCCGTCC TGGAGTTGGA 661 TGAGAGATAA TGGCCTTACG TTGTGCCAGG GGAGGGTCGG GCTGGATTTA GCNAAGATTT 721 ACCTTCTCCA AAGAGCGGTG CTGCAGTGGC ACAGCTGCCC ACGGAGGTGG GGGGGTCACC 781 GTCCCTGGAG GTGATGAANA ACTGTGGGGA NGTGGCACTG ANGGACATGG CCAGTGGGCN 841 NNNGNGGGTG GGGTTGGGGN NGGNCTTGGG NANCNNGGAG GGCTTTTNNN NNNTTCATGN 901 ANTTNNNCNA ATNNNATGAA NCNTCTACNT GGGNNNTTNN NCNNCTGNCN NGTCNCAGTC 961 NNACTGNNCN NANCTNNNNN NNNNN //
Primer: tetR-C_F2 (Pr0862) Region: tetR, SV40 NLS, KRAB Sequence file: RDB16905_A9Aff.seq
>D05911A3_A9Af_6_tetR-C_F2_G01_07_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNTCG GGCTNNNNNC ATTAATATTT 61 GGATTTTCTG CACAGTTNNA TGAAAAAAAG CATACACCCA TTGAGGATGG TAATAAATCG 121 CCAAAAAAGA AGAGAAAGGT CGATGGCGGT GGTGCTTTGT CTCCTCAGCA CTCTGCTGTC 181 ACTCAAGGAA GTATCATCAA GAACAAGGAG GGCATGGATG CTAAGTCACT AACTGCCTGG 241 TCCCGGACAC TGGTGACCTT CAAGGATGTA TTTGTGGACT TCACCAGGGA GGAGTGGAAG 301 CTGCTGGACA CTGCTCAGCA GATCGTGTAC AGAAATGTGA TGCTGGAGAA CTATAAGAAC 361 CTGGTTTCCT TGGGTTATCA GCTTACTAAG CCAGATGTGA TCCTCCGGTT GGAGAAGGGA 421 GAAGAGCCCT GGCTGGTGGA GAGAGAAATT CACCAAGAGA CCCATCCTGA TTCAGAGACT 481 GCATTTGAAA TCAAATCATC AGTTTAAGGA TCCGAGCTCG GTGATCCGCT GGCACGACAG 541 GTTTCCCGAC TGGAAAGCGG GCANNNNNNG CAACGCAATT AATGTGAGTT AGCTCACTCA 601 TTANGCACCC CAGGCNNNNN NNTTTATGCT TCCGGCTCGT ATGTTGTGTG GAATTGTGAG 661 CGGATAACAA TTTCANNNNN NAAACAGCTA TGACCNTGAT TACGCCAAGC TTGGGCTGCA 721 GCCATAGAGC CCACCGCATC CCCAGCATGN CNTGCTATTG TCTTCCCNAA TCCTCCCCCT 781 TGCTGTCCTG CCCCACCCCA NCCCCCANAA NAGAATGACN CCTACTCANA NNATGCNANN 841 NNATTTCCTC ANTTTATNAG GAANNGANAG TGGGNANNGG CNCCNTNNCA GGGTNNNAGG 901 NAGGCNCNGG GGGNNGGNNN AAANNANCAN ANGGCNGGNN AACNNNAANG NNNANTCNNN 961 GGCNNNATCA NNNNGCNNNN NNNATCATCN ANNNNCANNG GGGNNNNNNG NNNNNCCTTN 1021 NNNNNNGGNN NNNNNNGGNN NNNNGGGGGN NGNNNNNNNN GNNNNNNGGN NNNNNNNNN //
Primer: IRES-F (Pr0036) Region: IRES, tetR, SV40 NLS Sequence file: RDB16905_A9Afg.seq
>D05911A3_A9Af_6_IRES-F_H12_24_ABI24.ab1 1 NNNNNNNNNN NNNNNNNTNN GTCGAGGTTN NNNNNNGTCT AGGCCCCCCG AACNACGGGG 61 NCGTGGTTTT CCTTTGAAAA ACACGATGAT AATATGGCTA GATTAGATAA AAGTAAAGTG 121 ATTAACAGCG CATTAGAGCT GCTTAATGAG GTCGGAATCG AAGGTTTAAC AACCCGTAAA 181 CTCGCCCAGA AGCTTGGTGT AGAGCAGCCT ACATTGTATT GGCATGTAAA AAATAAGCGG 241 GCCCTTATGA ACATGCTTTC AGAGGCAATA CTGGCGAAGC ATCACACCCG TTCAGCACCG 301 TTACCGACTG AGAGTTGGCA GCAGTTTCTC CAGGAAAATG CTCTGAGTTT CCGTAAAGCA 361 TTACTGGTCC ATCGTGATGG AGCCCGATTG CATATAGGGA CCTCTCCTAC GCCCCCCCAG 421 TTTGAACAAG CAGAGGCGCA ACTACGCTGT CTATGCGATG CAGGGTTTTC GGTCGAGGAG 481 GCTCTTTTCA TTCTGCAATC TATCAGCCAT TTTACGTTGG GTGCAGTATT AGAGGAGCAA 541 GCAACAAACC AGATAGAAAA TAATCATGTG ATAGACGCTG CACCACCATT ATTACAAGAG 601 GCATTTAATA TTCAGGCGAG AACCTCTGCT GAAATGGCCT TCCATTTCGG GCTGAAATCA 661 TTAATATTTG GATTTTCTGC ACAGTTAGAT GAAAAAAAGC ATACACCCAT TGAGGATGGT 721 AATAAATCGC CAAAAAAGAA GAGAAAGGTC GATGGCGGTG GTGCTTTGTC TCCTCAGCAC 781 TCTGCTGTCA CTCAAGGAAG TATCATCAAG AACAAGGAGG GCATGGNTGC TAAGTCACTA 841 ACTGCCTGGT CCCNGACACT GGTGANCTTC AAGGANGTAT TTGTGGANNT CNNNNNGGAN 901 GANTGGAAGC TGCTGGANNC TGCTCANCAN ATCGNGTACA GAAATGTGAT GCTGGANAAC 961 TNTAANAANN NGGNTTCCNN GGGTNANCNN CNTNNTNANN NNGANGTGAT NNNNNNNNNN //
Primer: SV40pro_F_V2 (Pr0154) Region: SV40 pro_ori, rtTA-Advenced Sequence file: RDB16905_A9Afh.seq
>D05911A3_A9Af_6_SV40pro_F_V2_D11_11_ABI24.ab1 1 NNNNNNNNNN NNNTNNNNNN NNNNNNNNNC TTTTGCAAAA AGCTCTCTGG CTAACTAGAG 61 AACCCACTGC TTACTGGCTT ATCGAAATTA ATACGACTCA CTATAGGGAG ACCCAAGCTG 121 CAATTCACCA TGAGCAGACT GGACAAGAGC AAAGTCATAA ACGGCGCTCT GGAATTACTC 181 AATGGAGTCG GTATCGAAGG CCTGACGACA AGGAAACTCG CTCAAAAGCT GGGAGTTGAG 241 CAGCCTACCC TGTACTGGCA CGTGAAGAAC AAGCGGGCCC TGCTCGATGC CCTGCCAATC 301 GAGATGCTGG ACAGGCATCA TACCCACTTC TGCCCCCTGG AAGGCGAGTC ATGGCAAGAC 361 TTTCTGCGGA ACAACGCCAA GTCATTCCGC TGTGCTCTCC TCTCACATCG CGACGGGGCT 421 AAAGTGCATC TCGGCACCCG CCCAACAGAG AAACAGTACG AAACCCTGGA AAATCAGCTC 481 GCGTTCCTGT GTCAGCAAGG CTTCTCCCTG GAGAACGCAC TGTACGCTCT GTCCGCCGTG 541 GGCCACTTTA CACTGGGCTG CGTATTGGAG GAACAGGAGC ATCAAGTAGC AAAAGAGGAA 601 AGAGAGACAC CTACCACCGA TTCTATGCCC CCACTTCTGA GACAAGCAAT TGAGCTGTTC 661 GACCGGCAGG GAGCCGAACC TGCCTTCCTT TTCGGCCTGG AACTAATCAT ATGTGGCCTG 721 GAGAAACAGC TAAAGTGCGA AAGCGGCGGG CCGGCCGACG CCCTTGACGA TTTTGACTTA 781 NACATGCTCC CAGCCGATGC CCTTGACGAC TTTGANCTTG ATATGCTGCC TGCTGACGCT 841 CTTGACGANT TTGACCTTGA CATGCTCCCC GGGNAACTAA GTAAGGANCA TCCNCCCCCC 901 CCCCCTAACG TTACTGGNCG AANCCGCTNG NAATNNNGNC GGNGNGNGTT TGNCTATATG 961 TTATTTTNCN NCNTNNTGNC GNCTTTTNGN NNTGNGANGG NCNNGNAANN CNTNNNNNNN 1021 NNN //
Primer: SV40pA3'Element reverse (Pr0108) Region: Right Tol2, I-SceI, cHS4 ins_2 Sequence file: RDB16905_A9Afi.seq
>D05911A3_A9Af_6_SV40pA3'element Reverse_G12_21_ABI24.ab1 1 NNNNNNNNNN NNNNNNGNNN NNATGNNNNN NNNCNNGCAA CGCGGCCTTT TTACGGTTCC 61 TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG 121 ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC 181 GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCCAAT ACGCAAACCG CCTCTCCCCG 241 CGCGTTGGCC GATTCATTAA TGCAGCTGGC ACGACAGGTT TCCCGACTGG AAAGCGGGCA 301 GTGAGCGCAA CGCAATTAAT GTGAGTTAGC TCACTCATTA GGCACCCCAG GCTTTACACT 361 TTATGCTTCC GGCTCGTATG TTGTGTGGAA TTGTGAGCGG ATAACAATTT CACACAGGAA 421 ACAGCTATGA CCATGATTAC GCCAAGCTCG AAATTAACCC TCACTAAAGG GAACAAAAGC 481 TGGAGCTCCA GAGGTGTAAA AAGTACTCAA AAATTTTACT CAAGTGAAAG TACAAGTACT 541 TAGGGAAAAT TTTACTCAAT TAAAAGTAAA AGTATCTGGC TAGAATCTTA CTTGAGTAAA 601 AGTAAAAAAG TACTCCATTA AAATTGTACT TGAGTATTGG ATCAATTACC CTGTTATCCC 661 TATCGAGACG CGTCGGTCCG GCTAGAGCCC CATCCTCACT GACTCCGTCC TGGAGTTGGA 721 TGAGAGATAA TGGCCTTACG TTGTGCCAGG GGAGGGTCGG GCTGGATTTA GCAAGATTTA 781 CCTTCTCCAA AGAGCGGTGC TGCAGTGGCA CAGCTGCCCA CGGANGTNGG GGGGTCACCG 841 TCCCTGGANG TGATGAANAN NTGTGGGGAN GTGGCACTGA NGGANATGGC CAGTGGGCAC 901 GGNGGGTNGG TTGGGGTNNG NCTTGGGGNN CTTGGANGGC TTTTNCAGCC TTCATGATTT 961 GACNANTGTA TNNNNTCNNC NTGGCANNTN NNCNNCTGNN TGNCCNNNNN CNANTGNNCN 1021 ANCNNNNTCT CTNNNNNNNN NN //
Primer: SV40pro_R (Pr0470) Region: SV40 pro_ori, cHS4 ins_2 Sequence file: RDB16905_A9Afj.seq
>D05911A3_A9Af_6_SV40pro_R_C04_03_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNCNNN NNNNGNNNNN NANNNNGGGC GGANCTGGGC 61 GGANTTAGGG GCGNNNNGNG CGGAGTTAGG GGCGGGACTA TGGTTGCTGA CTAATTGAGA 121 TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCTGGTTGCT 181 GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA 241 CACCCTAACT GACACACATT CCACAGCCGT TAATTAAGAT CCCTAGAGGG ACAGCCCCCC 301 CCCAAAGCCC CCAGGGATGT AATTACGTCC CTCCCCCGCT AGGGGGCAGC AGCGAGCCGC 361 CCGGGGCTCC GCTCCGGTCC GGCGCTCCCC CCGCATCCCC GAGCCGGCAG CGTGCGGGGA 421 CAGCCCGGGC ACGGGGAAGG TGGCACGGGA TCGCTTTCCT CTGAACGCTT CTCGCTGCTC 481 TTTGAGCCTG CAGACACCTG GGGGGATACG GGGAAAAAGC TTTAGGCTGA AAGAGAGATT 541 TAGAATGACA GAATCNNNNN ACGGCCTGGG TTGCAAAGGA NNNNNNTGCT CATCCAGATC 601 CAACCCCCTG CTATGTGCAG GGTCATCAAC CAGCAGCCCA GGCTGCCCAG AGCCNNNTNN 661 NNNCTGGCCT TGAATGCCTG NNNNGATGGG GCATCCACNN NCTCCTTGGG CAACCTGTTC 721 NGTGCGTCAC CACCCTCTGG GGGAAAAAAN CTGCCTCCTC ATATCCAACC CNNAAACCTC 781 CCCTGNCTCA GTGNNAAAGC CATTCCCCCC NTTGTCCTAT CAAGGGGGGG AGTTTGCTGN 841 NNCATNGNTT GGTCTGGGNG GTGANNNCAT GTTNGNCNNA NTTCAGTNGC ATCNNNNNNN 901 NGNNNNATCT TTNNGGNATN AANGNNAGTN NCANGNNNNN CATGGNNNNN NGGNNNNNNG 961 NCNNNNNNNT TNNAANGGNC NNNNNGNNNN NNNNNN //

pT2b_mT_gvpC20-T2A-mKO2_pP_rm

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