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human TLR3 expression vector (#RDB16909)

Expression vector of human TLR3.

Clone info. Expression vector of human Toll-like receptor 3 (TLR3).
Comment Complementary DNAs for human TLR3 was cloned by RT-PCR from the mRNA of monocyte-derived immature DCs. Expression was confirmed by the depositor with western blotting with anti-TLR3 Ab, flowcytometry with anti-TLR3 mAb (clone TLR3.7).
Vector backbone pEFBOS (plasmid)
Selectable markers Amp^r
Gene/insert name human TLR3 cDNA
Depositor|Developer Matsumoto, Misako |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Matsumoto, M et al. Biochem. Biophys. Res. Commun. 293(5) : 1364-1369, 2002); (Matsumoto, M et al. Int. Immunol. 16(8) : 1143-1154, 2004)
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提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Matsumoto, M et al. Biochem. Biophys. Res. Commun. 293(5) : 1364-1369, 2002); (Matsumoto, M et al. Int. Immunol. 16(8) : 1143-1154, 2004)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB16909 human TLR3 expression vector DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The human TLR3 expression vector was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16909).

Reference section:

Matsumoto, M., Kikkawa, S., Kohase, M., Miyake, K., Seya, T., Establishment of a monoclonal antibody against human Toll-like receptor 3 that blocks double-stranded RNA-mediated signaling. Biochem. Biophys. Res. Commun. 293 (5): 1364-1369 (2002). PMID 12054664. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB16909_A9Ajp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F ()
Region: EF1alpha pro,insert5'
Sequence file: RDB16909_A9Aja.seq check
>D06031A1_A9Aj_1_EF1a-C_F_A04_01_ABI24.ab1
    1 TATGGGATGC CTTTTTGAGT TTGGATCTTG GTTCATTCTC AAGCCTCAGA CAGTGGTTCA
   61 AAGTTTTTTT CTTCCATTTC AGGTGTCGTG AGGAATTCTC TAGAGATCCC TCGACCTCGA
  121 GGCCGCCACC ATGAGACAGA CTTTGCCTTG TATCTACTTT TGGGGGGGCC TTTTGCCCTT
  181 TGGGATGCTG TGTGCATCCT CCACCACCAA GTGCACTGTT AGCCATGAAG TTGCTGACTG
  241 CAGCCACCTG AAGTTGACTC AGGTACCCGA TGATCTACCC ACAAACATAA CAGTGTTGAA
  301 CCTTACCCAT AATCAACTCA GAAGATTACC AGCCGCCAAC TTCACAAGGT ATAGCCAGCT
  361 AACTAGCTTG GATGTAGGAT TTAACACCAT CTCAAAACTG GAGCCAGAAT TGTGCCAGAA
  421 ACTTCCCATG TTAAAAGTTT TGAACCTCCA GCACAATGAG CTATCTCAAC TTTCTGATAA
  481 AACCTTTGCC TTCTGCACGA ATTTGACTGA ACTCCATCTC ATGTCCAACT CAATCCAGAA
  541 AATTAAAAAT AATCCCTTTG TCAAGCAGAA GAATTTAATC ACATTAGATC TGTCTCATAA
  601 TGGCTTGTCA TCTACAAAAT TAGGAACTCA GGTTCAGCTG GAAAATCTCC AAGAGCTTCT
  661 ATTATCAAAC AATAAAATTC AAGCGCTAAA AAGTGAAGAA CTGGATATCT TTGCCAATTC
  721 ATCTTTAAAA AAATTAGAGT TGTCATCGAA TCAAATTAAA GAGTTTTCTC CAGGGTGTTT
  781 TCACGCAATT GGAAGATTAT TTGGCCTCTT TCTGAACAAT GTCCAGCTGG GTCCCAGCCT
  841 TACAGAGAAG CTATGTTTGG AATTAGCAAA CACAAGCATT CGGAATCTGT CTCTGAGTAA
  901 CAGCCAGCTG TCCACCACCA GCAATACAAC TTTCTTGGGA CTAAAGTGGA CAAATCTCAC
  961 TATGCTCGAT CTTTCCTACA ACAACTTAAA TGTGGTTGGT AACGATTCCT TTGCTTGGCT
 1021 TCCACAACTA GAATATTTCT TTCCTAGAGT ATAATAATAT ACAGCATTTG TTTTTCTCAC
 1081 TTCTTTGCAC GGACTTTTTC AATGTGAGTA CCTGATTTGG AACGATCTTT TACCTAAACC
1141 AAAGGTATTT CCCTTGACCT CAACTCCCCC CAAAGAAATG GA
//
Primer: hGCSF_R ()
Region: hGCSF p(A),insert3'
Sequence file: RDB16909_A9Ajb.seq check
>D06031A1_A9Aj_1_hGCSF_R_B04_04_ABI24.ab1
    1 CTGGGAAATG ATCTCTCAGG GTCCCAGGTG GGGACCCTCA CTCTAGAGTC GCGGCCGCGA
   61 CCTGCAGGCG CAGAACTGGT AGGTATGGAA GATCCCTCGA GATCCATTGT GCTGGCGGTA
  121 GGCGAGCAGC GCCTGCCTGA AGCTGCGGGC ATTCCCGATC AGAAATGAGC GCCAGTCGTC
  181 GTCGGCTCTC GGCACCGAAT GCGTATGATT CTCCGCCAGC ATGGCTTCGG CCAGTGCGTC
  241 GAGCAGCGCC CGCTTGTTCC TGAAGTGCCA GTAAAGCGCC GGCTGCTGAA CCCCCAACCG
  301 TTCCGCCAGT TTGCGTGTCG TCAGACCGTC TACGCCGACC TCGTTCAACA GGTCCAGGGC
  361 GGCACGGATC ACTGTATTCG GCTGCAACTT TGTCATGCTT GACACTTTAT CACTGATAAA
  421 CATAATATGT CCACCAACTT ATCAGTGATA AAGAATCCGC GCCAGCACAA TGGATCTCGA
  481 GGAAATTCCG GAATTCGGCT TGTCAAAAAT AGGCCTGAAA TAAGGAGACA CATTTTCCTA
  541 GAAGAGATGT AATTGTGATA GAATATAATC ATTTACAAAT ATGAATAAAC AAATTATAAC
  601 ACCTTTATGG AAAACTTAAA TTTGCCATAG AACTTTTTAA ATTGAGAAAG TTTCTCCTTT
  661 GCTAATTGAA TATTTAAATA AATTTAATGT ACAGAGTTTT TGGATCCAAG TGCTACTTGC
  721 AATTTATGGC GAAAGGCACC TATCCGTTCT TTCTGAACTG GCCAGTTCAA GATGCAGTGA
  781 GATTTAAACA TTCCTCTTCG CAAACAGAGT GCATGGTTCA GTTTATAATC TGGAATCTCC
  841 TCAAGGAAAA CCAATATAAT GGAATCCAGA TTTTGTTCAA TAGCTTGTTG AACTGCATGA
  901 TGTACCTTGA ATCTTTTGCA TAATGGGTCT TTTAATAGAT GGTGTGTTAT AACAAAAATA
  961 ATTTTTCTGC TTCTTTTGAT GCTGTTAACA ATTGCTTTCT AGTTCAAAAA CACCCGCCTC
 1021 AAAGTCCCTT TCTTCCAGAC AAAATTTGAG AGATGGTTCT TCCTTTTCCA TTTGAAGAGG
 1081 AAATGTTCCC AGACCCAAAT CCTTATTCTT TATAGCCATG ATTATATATG CCTGCCATAT
1141 CAAACTGTTC TGGTCCTGGT TCTGGTCTTA TTTC
//
Primer: reverse ()
Region: SV40pro_ori,EF1alpha pro
Sequence file: RDB16909_A9Ajc.seq check
>D06031A1_A9Aj_1_reverse_C04_07_ABI24.ab1
    1 CATAGTCCTC CCAGCAGGCA GAAGTATGCA AAGCATGCAT CTCAATTAGT CAGCAACCAT
   61 AGTCCCGCCC CTAACTCCGC CCATCCCGCC CCTAACTCCG CCCAGTTCCG CCCATTCTCC
  121 GCCCCATGGC TGACTAATTT TTTTTATTTA TGCAGAGGCC GAGGCCGCCT CGGCCTCTGA
  181 GCTATTCCAG AAGTAGTGAG GAGGCTTTTT TGGAGGCCTA GGCTTTTGCA AAAAGCTTTG
  241 CAAAGATGGA TAAAGTTTTA AACAGAGAGG AATCTTTGCA GCTAATGGAC CTTCTAGGTC
  301 TTGAAAGGAG TGGGAATTGG CTCCGGTGCC CGTCAGTGGG CAGAGCGCAC ATCGCCCACA
  361 GTCCCCGAGA AGTTGGGGGG AGGGGTCGGC AATTGAACCG GTGCCTAGAG AAGGTGGCGC
  421 GGGGTAAACT GGGAAAGTGA TGTCGTGTAC TGGCTCCGCC TTTTTCCCGA GGGTGGGGGA
  481 GAACCGTATA TAAGTGCAGT AGTCGCCGTG AACGTTCTTT TTCGCAACGG GTTTGCCGCC
  541 AGAACACAGG TAAGTGCCGT GTGTGGTTCC CGCGGGCCTG GCCTCTTTAC GGGTTATGGC
  601 CCTTGCGTGC CTTGAATTAC TTCCACCTGG CTGCAGTACG TGATTCTTGA TCCCGAGCTT
  661 CGGGTTGGAA GTGGGTGGGA GAGTTCGAGG CCTTGCGCTT AAGGAGCCCC TTCGCCTCGT
  721 GCTTGAGTTG AGGCCTGGCC TGGGCGCTGG GGCCGCCGCG TGCGAATCTG GTGGCACCTT
  781 CGCGCCTGTC TCGCTGCTTT CGATAAGTCT CTAGCCATTT AAAATTTTTG ATGACCTGCT
  841 GCGACGCTTT TTTTCTGGCA AGATAGTCTT GTAAATGCGG GCCAAGATCT GCACACTGGT
  901 ATTTCGGTTT TTGGGGCCGC GGGCGGCGAC GGGGCCCGTG CGTCCCAGCG CACATGTTCG
  961 GCGAGGCGGG GCCTGCGAGC GCGGGCCACC GAGAATCGGG ACGGGGGGTA GTCTCAAGCT
 1021 GGGCCGGGCC TGCTCTGGGT GCCTGACCTC GCGCCGTCGT GTATCGCCCC GCCTGGCGGC
 1081 CATGCTGGTC TGTCGGCACA GTTGCGTGAA CGGAAGATGG ACGCGTCCTG ATCCTGCCTG
1141 CCAGGTAGCT TCAAAATGTG TAAGGAACGC T
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Matsumoto, M., Establishment of a monoclonal antibody against human Toll-like receptor 3 that blocks double-stranded RNA-mediated signaling. Biochem. Biophys. Res. Commun. 293 (5): 1364-1369 (2002). PMID 12054664. [link to RRC of NBRP]