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human TLR3-CFlag expression vector

Expression vector of human TLR3 tagged with FLAG at C-terminus.

Catalog number RDB16911
Resource name human TLR3-CFlag expression vector
Clone info. Expression vector of human Toll-like receptor 3 (TLR3) tagged with FLAG at C-terminus.
Comment Complementary DNAs for human TLR3 was cloned by RT-PCR from the mRNA of monocyte-derived immature DCs. Expression was confirmed by the depositor with immunoprecipitation and western blotting.
Vector backbone pEFBOS (plasmid)
Selectable markers Amp^r
Gene/insert name human TLR3 cDNA
Depositor|Developer Matsumoto, Misako |
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External Database
human TLR3

          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Matsumoto, M et al. Biochem. Biophys. Res. Commun. 293(5) : 1364-1369, 2002); (Matsumoto, M et al. Int. Immunol. 16(8) : 1143-1154, 2004)
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Matsumoto, M et al. Biochem. Biophys. Res. Commun. 293(5) : 1364-1369, 2002); (Matsumoto, M et al. Int. Immunol. 16(8) : 1143-1154, 2004)。
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          RDB16911 human TLR3-CFlag expression vector DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The human TLR3-CFlag expression vector was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16911).

          Reference section:

          Matsumoto, M., Kikkawa, S., Kohase, M., Miyake, K., Seya, T., Establishment of a monoclonal antibody against human Toll-like receptor 3 that blocks double-stranded RNA-mediated signaling. Biochem. Biophys. Res. Commun. 293 (5): 1364-1369 (2002). PMID 12054664. [link to RRC of NBRP]

          Further references such as user reports and related articles (go to bottom)


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          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB16911_A9Ajp1-3.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: EF1a-C_F (Pr0168)
          Region: EF-1 alpha pro,insert 5'(TLR3 5')
          Sequence file: RDB16911_A9Ajd.seq check
          >D06031A3_A9Aj_2_EF1a-C_F_A05_02_ABI24.ab1
              1 NNNNNNNNNN NNTTTTGAGT TTGGNNCTTG GTTCNTTCTC NAGCCTCAGA CAGTGGTTCA
             61 AAGTTTTTTT CTTCCNTTTC AGGTGTCGTG AGGAATTCTC TAGAGATCCC TCGACCTCGA
            121 GGCCGCCACC ATGAGACAGA CTTTGCCTTG TATCTACTTT TGGGGGGGCC TTTTGCCCTT
            181 TGGGATGCTG TGTGCATCCT CCACCACCAA GTGCACTGTT AGCCATGAAG TTGCTGACTG
            241 CAGCCACCTG AAGTTGACTC AGGTACCCGA TGATCTACCC ACAAACATAA CAGTGTTGAA
            301 CCTTACCCAT AATCAACTCA GAAGATTACC AGCCGCCAAC TTCACAAGGT ATAGCCAGCT
            361 AACTAGCTTG GATGTAGGAT TTAACACCAT CTCAAAACTG GAGCCAGAAT TGTGCCAGAA
            421 ACTTCCCATG TTAAAAGTTT TGAACCTCCA GCACAATGAG CTATCTCAAC TTTCTGATAA
            481 AACCTTTGCC TTCTGCACGA ATTTGACTGA ACTCCATCTC ATGTCCAACT CAATCCAGAA
            541 AATTAAAAAT AATCCCTTTG TCAAGCAGAA GAATTTAATC ACATTAGATC TGTCTCATAA
            601 TGGCTTGTCA TCTACAAAAT TAGGAACTCA GGTTCAGCTG GAAAATCTCC AAGAGCTTCT
            661 ATTATCAAAC AATAAAATTC AAGCGCTAAA AAGTGAAGAA CTGGATATCT TTGCCAATTC
            721 ATCTTTAAAA AAATTAGAGT TGTCATCGAA TCAAATTAAA GAGTTTTCTC CAGGGTGTTT
            781 TCACGCAATT GGAAGANTAT TTGGCCTCTT TCTGAACAAT GTCCAGCTGG GTCCCAGCCT
            841 TACAGANAAN CTATGTTTGG AATTAGCAAA CACAAGCATT CGGAATCTGT CTCTGANTAA
            901 CAGCCAGCTG TCCNCCACCA GNNATACNAC TTTCTNGGNN NTNAAGTGGN NAAATCTCAC
            961 TANGCTCNNT CTTTCCNANN NCNACTTAAA NGNGGNNGNN NNCGANNNCN TNGNNNNNNN
          1021 TCCNNNNNNA NNNNNNTTNN NNNCNNNNNN NNNN
          //
          Primer: hGCSF_R (Pr0031)
          Region: insert 3'(FLAG,TLR3 3')
          Sequence file: RDB16911_A9Aje.seq check
          >D06031A3_A9Aj_2_hGCSF_R_B05_05_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNAN GGTCNNNGGT GGGGNNCCTC ACTCTNNGAG TCGCGGCCGC
             61 TTACTTGTCA TCGTCGTCCT TGTAGTCATG TACAGAGTTT TTGGATCCAA GTGCTACTTG
            121 CAATTTATGG CGAAAGGCAC CTATCCGTTC TTTCTGAACT GGCCAGTTCA AGATGCAGTG
            181 AGATTTAAAC ATTCCTCTTC GCAAACAGAG TGCATGGTTC AGTTTATAAT CTGGAATCTC
            241 CTCAAGGAAA ACCAATATAA TGGAATCCAG ATTTTGTTCA ATAGCTTGTT GAACTGCATG
            301 ATGTACCTTG AATCTTTTGC ATAATGGGTC TTTTAATAGA TGGTGTGTTA TAACAAAAAT
            361 AATTTTTCTG CTTCTTTTGA TGCTGTTAAC AATTGCTTCT AGTTCAAAAA CACCCGCCTC
            421 AAAGTCCCTT TCTTCCAGAC AAAATTTGAG AGATTGGTCT TCCTTTTCCA TTGAAGAGAA
            481 ATGTTCCCAG ACCCAATCCT TATCTTTATA GGCATGAATT ATATATGCTG CATATTCAAA
            541 CTGTTCTGTC TGTCTGTCTA TTTCTTTGAA ACCAAGAACT CGATGTACTG AAACATTCCA
            601 ATAAAAAGAT ATCCTCCAGC CCTCAAAGTG GATGAGAAGT ACAATAAAGA TAAAAATCAA
            661 CAGGATACTG GTATTGATCA TGAAAAAGAG TTCAAAGGGG GCACTGTCTT TGCAAGATGA
            721 TGTATCAAAA AGTCTCACTG GGAACCCATG ATAGTGAGGT GGAGTGTTGC AAANNNAGTG
            781 GCTTGACAGC TCAGGGATGT TGGTATGGGT CTCGTTAATC NNATTAACAA ACCAGGCAAT
            841 ACTTTCACAC GTGCAATCAA AGGGANTAAA GCGCATATCT NACTCNGTCA GGNTNCTGAA
            901 AGCTGGNCCG AAAANNTTCT NCTCNNCNNA TGTTATGANA NNCTTCTGAA NNNTNANTGN
            961 NTTTNNAGAN NNCTGANTAT TAAAGANAGA TGCTGGNANN GTGTTTNAAN TNATTNNNTC
          1021 CNAAATNGAN GATNNTTANN NNNNNNANNN CNNNNNNNNN N
          //
          Primer: reverse (Pr0099)
          Region: SV40 pro_ori,EF-1 alpha pro
          Sequence file: RDB16911_A9Ajf.seq check
          >D06031A3_A9Aj_2_reverse_C05_08_ABI24.ab1
              1 NNNNNNNNNN NNNNNANGCA GAAGTANGCN AAGCATGCAT CTCNATTAGT CAGCAACCAT
             61 AGTCCCGCCC CTAACTCCGC CCATCCCGCC CCTAACTCCG CCCAGTTCCG CCCATTCTCC
            121 GCCCCATGGC TGACTAATTT TTTTTATTTA TGCAGAGGCC GAGGCCGCCT CGGCCTCTGA
            181 GCTATTCCAG AAGTAGTGAG GAGGCTTTTT TGGAGGCCTA GGCTTTTGCA AAAAGCTTTG
            241 CAAAGATGGA TAAAGTTTTA AACAGAGAGG AATCTTTGCA GCTAATGGAC CTTCTAGGTC
            301 TTGAAAGGAG TGGGAATTGG CTCCGGTGCC CGTCAGTGGG CAGAGCGCAC ATCGCCCACA
            361 GTCCCCGAGA AGTTGGGGGG AGGGGTCGGC AATTGAACCG GTGCCTAGAG AAGGTGGCGC
            421 GGGGTAAACT GGGAAAGTGA TGTCGTGTAC TGGCTCCGCC TTTTTCCCGA GGGTGGGGGA
            481 GAACCGTATA TAAGTGCAGT AGTCGCCGTG AACGTTCTTT TTCGCAACGG GTTTGCCGCC
            541 AGAACACAGG TAAGTGCCGT GTGTGGTTCC CGCGGGCCTG GCCTCTTTAC GGGTTATGGC
            601 CCTTGCGTGC CTTGAATTAC TTCCACCTGG CTGCAGTACG TGATTCTTGA TCCCGAGCTT
            661 CGGGTTGGAA GTGGGTGGGA GAGTTCGAGG CCTTGCGCTT AAGGAGCCCC TTCGCCTCGT
            721 GCTTGAGTTG AGGCCTGGCC TGGGCGCTGG GGCCGCCGCG TGCGAATCTG GTGGCACCTT
            781 CGCGCCTGTC TCGCTGCTTT CGATAAGTCT CTAGCCNTTT AAAATTTTTG ATGACCTGCT
            841 GCGACGCTTT TTTTCTGGCN AGATAGTCTT GTAAATGCGG GCCNAGATCT GNACACTGGT
            901 ATTTCGGNTT TTGGGGNCGC GGGCGGCGAN NGGGCCNNNN CGNNCCNNCG CNCATGTTCN
            961 GNGANNNGGG GNCNGCNANC NNGGNNNNNN ANNNNNNNNN GGGGNNANNN NNNNNCNNGN
          1021 NNNGNNNNGN NNNNGNNNNN NNNNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Matsumoto, M., Establishment of a monoclonal antibody against human Toll-like receptor 3 that blocks double-stranded RNA-mediated signaling. Biochem. Biophys. Res. Commun. 293 (5): 1364-1369 (2002). PMID 12054664. [link to RRC of NBRP]

          2024.03.26

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