Please review the QC test results indicated by icon below as well as clone information before placing your order.

human TLR8-CFlag expression vector

Expression vector of human TLR8 tagged with FLAG at C-terminus.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Itoh, H et al. PLoS One. 6(12) : e28500, 2011); (Ishii, N et al. J. Immunol. 193(10) : 5118-5128, 2014)
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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Itoh, H et al. PLoS One. 6(12) : e28500, 2011); (Ishii, N et al. J. Immunol. 193(10) : 5118-5128, 2014)。
提供依頼	提供依頼書 [Word] 提供同意書 (MTA, 非営利学術目的用)[Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

Reference section:

Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB16912_A9Ajp1-3.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F (Pr0168) Region: EF-1 alpha pro,insert 5'(TLR8 5') Sequence file: RDB16912_A9Ajd.seq
>D06031A4_A9Aj_2_EF1a-C_F_D05_11_ABI24.ab1     1 NNNNNNNNNT NGNNNNTTTT NANTTTGGNN CTTGGTTCNT TCTCNAGCCT CAGACAGTGG    61 TTCAAAGTTT TTTTCTTCCA TTTCAGGTGT CGTGAGGAAT TCTCTAGAGA TCCCTCGACC   121 TCGAGGCCAC CATGAAGGAG TCATCTTTGC AAAATAGCTC CTGCAGCCTG GGAAAGGAGA   181 CTAAAAAGGA AAACATGTTC CTTCAGTCGT CAATGCTGAC CTGCATTTTC CTGCTAATAT   241 CTGGTTCCTG TGAGTTATGC GCCGAAGAAA ATTTTTCTAG AAGCTATCCT TGTGATGAGA   301 AAAAGCAAAA TGACTCAGTT ATTGCAGAGT GCAGCAATCG TCGACTACAG GAAGTTCCCC   361 AAACGGTGGG CAAATATGTG ACAGAACTAG ACCTGTCTGA TAATTTCATC ACACACATAA   421 CGAATGAATC ATTTCAAGGG CTGCAAAATC TCACTAAAAT AAATCTAAAC CACAACCCCA   481 ATGTACAGCA CCAGAACGGA AATCCCGGTA TACAATCAAA TGGCTTGAAT ATCACAGACG   541 GGGCATTCCT CAACCTAAAA AACCTAAGGG AGTTACTGCT TGAAGACAAC CAGTTACCCC   601 AAATACCCTC TGGTTTGCCA GAGTCTTTGA CAGAACTTAG TCTAATTCAA AACAATATAT   661 ACAACATAAC TAAAGAGGGC ATTTCAAGAC TTATAAACTT GAAAAATCTC TATTTGGCCT   721 GGAACTGCTA TTTTAACAAA GTTTGCGAGA AAACTAACAT AGAAGANGGA GTATTTGAAA   781 CGCTGACAAA TTTGGAGTTG CTATCACTAT CTTTCAATTC TCTTTCACAC GTGCCACCNA   841 AACTGCCAAG CTCCNTACGC AAACTTTTTC TGANCAACAC CCAGATCNAA TACNTTAGTG   901 AAGAANANTN CNAGGGNNTG ATAAATTTNA CATTACNAGA NTTAAGCGGG AACTGTCCNA   961 NGNGCTTCNN NGNCCNATTN NNNNGCGNGN CNTNGNNANG GNGGNGCTTC ANTTNATATA 1021 NATCGNTTTN GNTTTNNNAA ANNTNNNNCN NNNNTNNNNN NNNNN //
Primer: hGCSF_R (Pr0031) Region: insert 3'(FLAG,TLR8 3') Sequence file: RDB16912_A9Aje.seq
>D06031A4_A9Aj_2_hGCSF_R_E05_14_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNGGTCNCNG GTGGGGNNCC TCACTCTNNG AGTCGCGGCC    61 GCTTACTTGT CGTCATCGTC TTTGTAGTCG TATTGCTTAA TGGAATCGAC ATACATATTG   121 TTATACCGTG AATCATTTTC AGTCAAGACC ACATTTCTCA GAGTTTGCCA AAACAAGCCT   181 TCTGCCTTCG GGTTGTCAGG CCACTGGAGG ATGGAGCTCT TACAGATCCG CTGCCGTAGC   241 CTCAAATACT GAGAATGCTG TAACACTGGC TCCAGCAGGA TAAATATAAT CACATCCATG   301 TTCTCATCCA TTAGCCTCTG CAAAGCCAAG TAAAAAGCTG TTTTAAAGTT CCAGCTTTTT   361 GCATATTTTT TGGTTAAAAC AAATACTGTT TTCTTGCTTT GGTTGATGCT CTGCATGAGG   421 TTGTCGATGA TGGCCAATCC CGGGTCCCAA TCCCTCTCCT CTAGACAAAG GAGAACGTTT   481 TTGTCTCGGC TCTCTTCAAG GTGGTAGCGC AGCTCATTTA TCACCCAGTC AGTAACAGAG   541 GCATCTTTGG TGTCATAAGA AATGTAAGCA TCATAGAAAG TTTGGGATGT GGAAAGAGAC   601 CTGTAGCCTT TTACCTTAGC TAAACACACA TTATATATAA ACCAAACATC CCAGTAAAAC   661 AAATGGTGAG CCAGGGCAGC CAACATAACC ATGGTGGTGA TAAAGAACGT GAAGAAAAAT   721 AATATCACTG CAGTGACATC TGAAACACAA GTTGTTAGCT CCNGACTCAC AATACTCTTC   781 CCTCTTTGAT CCCCAGGANT GGCACAAATG ACATCTACCA GTCTGGGAAT TTTGACATTC   841 AGATGTTCAT CCNTCCATCT TCGGAAATCT CCAATGTCAC AGGNGCATTN NAAGGGGTTT   901 CCGNGTANTT NCNACATAGA TAATTTGGTG GNGGTCTTAN TTTCAAGTGN GGNNTTGNTT   961 ANNGNTTTTA NCNNANTGGN ANNNNNAATN NANGTGNTTC NNANNACTGA CTTCNNAAAN 1021 AAANNNNNNN GGNAGGTGGN NAANNNNGNN ATGANNNNNN NNNN //
Primer: reverse (Pr0099) Region: SV40 pro_ori,EF-1 alpha pro Sequence file: RDB16912_A9Ajf.seq
>D06031A4_A9Aj_2_reverse_F05_17_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNAG GCAGAAGTNN GCNAAGCATG CATCTCNATT AGTCNGCAAC    61 CATAGTCCCG CCCCTAACTC CGCCCATCCC GCCCCTAACT CCGCCCAGTT CCGCCCATTC   121 TCCGCCCCAT GGCTGACTAA TTTTTTTTAT TTATGCAGAG GCCGAGGCCG CCTCGGCCTC   181 TGAGCTATTC CAGAAGTAGT GAGGAGGCTT TTTTGGAGGC CTAGGCTTTT GCAAAAAGCT   241 TTGCAAAGAT GGATAAAGTT TTAAACAGAG AGGAATCTTT GCAGCTAATG GACCTTCTAG   301 GTCTTGAAAG GAGTGGGAAT TGGCTCCGGT GCCCGTCAGT GGGCAGAGCG CACATCGCCC   361 ACAGTCCCCG AGAAGTTGGG GGGAGGGGTC GGCAATTGAA CCGGTGCCTA GAGAAGGTGG   421 CGCGGGGTAA ACTGGGAAAG TGATGTCGTG TACTGGCTCC GCCTTTTTCC CGAGGGTGGG   481 GGAGAACCGT ATATAAGTGC AGTAGTCGCC GTGAACGTTC TTTTTCGCAA CGGGTTTGCC   541 GCCAGAACAC AGGTAAGTGC CGTGTGTGGT TCCCGCGGGC CTGGCCTCTT TACGGGTTAT   601 GGCCCTTGCG TGCCTTGAAT TACTTCCACC TGGCTGCAGT ACGTGATTCT TGATCCCGAG   661 CTTCGGGTTG GAAGTGGGTG GGAGAGTTCG AGGCCTTGCG CTTAAGGAGC CCCTTCGCCT   721 CGTGCTTGAG TTGAGGCCTG GCCTGGGCGC TGGGGCCGCC GCGTGCGAAT CTGGTGGCAC   781 CTTCGCGCCT GTCTCGCTGC TTTCGATAAG TCTCTAGCCA TTTAAAATTT TTGATGACCT   841 GCTGCGACGC TNTTTTTCTG GNAAGATAGT CTTGTAAATG CGNGCCNAGA TCTGNACACN   901 GGNATTTCGG TTTTTGGGGN CGCGGGCNNC GANNGGGCCN GTGCGNCCCN NCGCNCNTGN   961 TCGGNGANNN GGGGNNNGCN ANNNNNGNNN NNNNANAANN NNNNNGGGGT ANNNNCNNNC 1021 NNGNNNNGNN NNNNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2024.03.26

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