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human TICAM-2 expression vector (#RDB16924)

Expression vector of human TICAM-2.

Clone info. Expression vector of human Toll-interleukin 1 receptor domain (TIR)-containing adaptor molecule 2 (TICAM-2).
Comment The full-length human TICAM-2 cDNA was obtained from MRC-5 cDNA by RT-PCR. Expession was confirmed by the depositor.
Vector backbone pEFBOS (plasmid)
Selectable markers Amp^r
Gene/insert name human TICAM2 cDNA
Depositor|Developer Seya, Tsukasa |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Oshiumi, H. et al. J. Biol. Chem. 12; 278(50) : 49751-49762, 2003) and an acknowledgment to the DEPOSITOR are requested. Basic research use only. Do not pass the plasmids to the third persons without permission.
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Oshiumi, H. et al. J. Biol. Chem. 12; 278(50) : 49751-49762, 2003) を引用し、謝辞の表明を必要とする。基礎研究に限る。許可なく第3者に渡すことを禁じる。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB16924 human TICAM-2 expression vector DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The human TICAM-2 expression vector was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16924).

Reference section:

Oshiumi, H., Sasai, M., Shida, K., Fujita, T., Matsumoto, M., Seya, T., TIR-containing adapter molecule (TICAM)-2, a bridging adapter recruiting to toll-like receptor 4 TICAM-1 that induces interferon-beta. J. Biol. Chem. 278 (50): 49751-49762 (2003). PMID 14519765. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB16924_A9B7p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F ()
Region: EF-1a pro, insert 5'
Sequence file: RDB16924_A9B7a.seq check
>D06030A2_A9A7_1_EF1a-C_F_E01_13_ABI24.ab1
    1 TATTGGATTT GGCCTTTTTG AGTTTGGATC TTGGTTCATT CTCAAGCCTC AGACAGTGGT
   61 TCAAAGTTTT TTTCTTCCAT TTCAGGTGTC GTGAGGAATT CTCTAGAGAT CCCTCGACCT
  121 CGAGCCACCA TGGGTATCGG GAAGTCTAAA ATAAATTCCT GCCCTCTTTC TCTCTCTTGG
  181 GGTAAAAGGC ACAGTGTGGA TACAAGTCCA GGATATCATG AGTCAGATTC CAAGAAGTCT
  241 GAAGATCTAT CCTTGTGTAA TGTTGCTGAG CACAGCAATA CAACAGAGGG GCCAACAGGA
  301 AAGCAGGAGG GAGCTCAGAG CGTGGAAGAG ATGTTTGAAG AAGAAGCTGA AGAAGAGGTG
  361 TTCCTCAAAT TTGTGATATT GCATGCAGAA GATGACACAG ATGAAGCCCT CAGAGTCCAG
  421 AATCTGCTAC AAGATGACTT TGGTATCAAA CCCGGAATAA TCTTTGCTGA GATGCCATGT
  481 GGCAGACAGC ATTTACAGAA TTTAGATGAT GCTGTAAATG GGTCTGCATG GACAATCTTA
  541 TTACTGACTG AAAACTTTTT AAGAGATACT TGGTGTAATT TCCAGTTCTA TACGTCCCTA
  601 ATGAACTCCG TTAACAGGCA GCATAAATAC AACTCTGTTA TACCCATGCG GCCCCTGAAC
  661 AATCCCCTTC CCCGAGAAAG GACTCCCTTT GCCCTCCAAA CCATCAATGC CTTAGAGGAA
  721 GAAAGTCGTG GATTTCCTAC ACAAGTAGAA AGAATTTTTC AGGAGTCTGT GTATAAGACA
  781 CAACAAACTA TATGGAAAGA GACAAGAAAT ATGGTACAAA GACAATTTAT TGCCTGAGAT
  841 GAAACATATA ACCTGCAGCC CCTGAATTCC TGCAGCCCGG GGGGATCCAC TAGTTCTAGA
  901 GCGGCCGCGA CTCTAGAGTG AGGGTCCCCA CCCTGGGACC CTTGAGAGTA TCACGTCTCC
  961 CACGTGGGAG ACAAGAAAAT CCCTGTTTAA ATATTTAAAC AGCAAGTGTT CCCCATCTGG
 1021 GTCCCTTTGC ACCCCTCACT CCTGGTCCTC AGCCGAACTG CACAGTGGAC CCATGCCAAT
1081 CCCCCTTGAC CTGTTGA
//
Primer: hGCSF_R ()
Region: hGCSF 3'LTR + poly(A), insert 3'
Sequence file: RDB16924_A9B7b.seq check
>D06030A2_A9A7_1_hGCSF_R_F01_16_ABI24.ab1
    1 GGGGGGACTG CATCTCTCAG GGTCCAGGTG GGGACCCTCA CTCTAGAGTC GCGGCCGCTC
   61 TAGAACTAGT GGATCCCCCG GGCTGCAGGA ATTCAGGGGC TGCAGGTTAT ATGTTTCATC
  121 TCAGGCAATA AATTGTCTTT GTACCATATT TCTTGTCTCT TTCCATATAG TTTGTTGTGT
  181 CTTATACACA GACTCCTGAA AAATTCTTTC TACTTGTGTA GGAAATCCAC GACTTTCTTC
  241 CTCTAAGGCA TTGATGGTTT GGAGGGCAAA GGGAGTCCTT TCTCGGGGAA GGGGATTGTT
  301 CAGGGGCCGC ATGGGTATAA CAGAGTTGTA TTTATGCTGC CTGTTAACGG AGTTCATTAG
  361 GGACGTATAG AACTGGAAAT TACACCAAGT ATCTCTTAAA AAGTTTTCAG TCAGTAATAA
  421 GATTGTCCAT GCAGACCCAT TTACAGCATC ATCTAAATTC TGTAAATGCT GTCTGCCACA
  481 TGGCATCTCA GCAAAGATTA TTCCGGGTTT GATACCAAAG TCATCTTGTA GCAGATTCTG
  541 GACTCTGAGG GCTTCATCTG TGTCATCTTC TGCATGCAAT ATCACAAATT TGAGGAACAC
  601 CTCTTCTTCA GCTTCTTCTT CAAACATCTC TTCCACGCTC TGAGCTCCCT CCTGCTTTCC
  661 TGTTGGCCCC TCTGTTGTAT TGCTGTGCTC AGCAACATTA CACAAGGATA GATCTTCAGA
  721 CTTCTTGGAA TCTGACTCAT GATATCCTGG ACTTGTATCC ACACTGTGCC TTTTACCCCA
  781 AGAGAGAGAA AGAGGGCAGG AATTTATTTT AGACTTCCCG ATACCCATGG TGGCTCGAGG
  841 TCGAGGGATC TCTAGAGAAT TCCTCACGAC ACCTGAAATG GAAGAAAAAA ACTTTGAACC
  901 ACTGTCTGAG GCTTGAGAAT GAACCAAGAT CCAAACTCAA AAAGGGCAAA TTTCCAAAGG
  961 AGAATTTACA TCAAGGTGCC CAAGCTGGGC CTAACTTTCA GTCTCCACCC ACTTCAGTGT
 1021 GGGGGAAGCT CCATTCGCAT TAAAACCCTT CCCCCCCAAC CTAGACGACG GTAACTTCGA
1081 AAGGCACGAG AAACCTAAAA TTCCG
//
Primer: pT2KXIG#1 ()
Region: SV40pro/ori, EF-1a pro
Sequence file: RDB16924_A9B7c.seq check
>D06030A2_A9A7_1_pT2KXIG#1_F03_18_ABI24.ab1
    1 AAAAAATTCC CTTCCCCAGC AGGCAGAAGT ATGCAAAGCA TGCATCTCAA TTAGTCAGCA
   61 ACCATAGTCC CGCCCCTAAC TCCGCCCATC CCGCCCCTAA CTCCGCCCAG TTCCGCCCAT
  121 TCTCCGCCCC ATGGCTGACT AATTTTTTTT ATTTATGCAG AGGCCGAGGC CGCCTCGGCC
  181 TCTGAGCTAT TCCAGAAGTA GTGAGGAGGC TTTTTTGGAG GCCTAGGCTT TTGCAAAAAG
  241 CTTTGCAAAG ATGGATAAAG TTTTAAACAG AGAGGAATCT TTGCAGCTAA TGGACCTTCT
  301 AGGTCTTGAA AGGAGTGGGA ATTGGCTCCG GTGCCCGTCA GTGGGCAGAG CGCACATCGC
  361 CCACAGTCCC CGAGAAGTTG GGGGGAGGGG TCGGCAATTG AACCGGTGCC TAGAGAAGGT
  421 GGCGCGGGGT AAACTGGGAA AGTGATGTCG TGTACTGGCT CCGCCTTTTT CCCGAGGGTG
  481 GGGGAGAACC GTATATAAGT GCAGTAGTCG CCGTGAACGT TCTTTTTCGC AACGGGTTTG
  541 CCGCCAGAAC ACAGGTAAGT GCCGTGTGTG GTTCCCGCGG GCCTGGCCTC TTTACGGGTT
  601 ATGGCCCTTG CGTGCCTTGA ATTACTTCCA CCTGGCTGCA GTACGTGATT CTTGATCCCG
  661 AGCTTCGGGT TGGAAGTGGG TGGGAGAGTT CGAGGCCTTG CGCTTAAGGA GCCCCTTCGC
  721 CTCGTGCTTG AGTTGAGGCC TGGCCTGGGC GCTGGGGCCG CCGCGTGCGA ATCTGGTGGC
  781 ACCTTCGCGC CTGTCTCGCT GCTTTCGATA AGTCTCTAGC CATTTAAATT TTTGATGACC
  841 TGCTGCGACG CTTTTTTTTC TGGCAAGATA GTCTTGTAAA TGCGGGCCAA GATCTGCACA
  901 CTGGGTATTT CGGTTTTTTG GGGCCGCGGG CGGCGACGGG GGCCGTGCGT CCCAAGCGCA
  961 CATGTTTCGG CGAGGCGGGG TCTGCGGAGC GGCGGCCCAC CGAGGAATCG GAACGGGGGT
 1021 AGGTCTTCAA GGCTGACCGG ACCTGCTTCC TGGGTGCCTG GACTTCGCGT CGATTCGATG
1081 TTATCGGCCC CCGGCCCCTT G
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Oshiumi, H., TIR-containing adapter molecule (TICAM)-2, a bridging adapter recruiting to toll-like receptor 4 TICAM-1 that induces interferon-beta. J. Biol. Chem. 278 (50): 49751-49762 (2003). PMID 14519765. [link to RRC of NBRP]