Please review the QC test results indicated by icon below as well as clone information before placing your order.

pMRX-puro-EGFP-Rab10

Retroviral expression vector of mouse Rab10, fused with EGFP.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kuroda, T. S. et al. (2002) J. Biol. Chem. 277, 9212-9218); (Etoh, K. and Fukuda, M. (2019) J. Cell Sci. 132, jcs226977) Additional terms and conditions: Regarding pMRX vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literatures for pMX and pMRX vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003; Saitoh T., J. Biol. Chem. 278 (38): 36005-36012, 2003) is requested.
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
Remarks	Remember that you will be working with samples containing infectious virus.

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Kuroda, T. S. et al. (2002) J. Biol. Chem. 277, 9212-9218); (Etoh, K. and Fukuda, M. (2019) J. Cell Sci. 132, jcs226977)。 付加的提供条件： pMRXベクターについて：利用者は、研究成果の公表にあたって pMXならびにpMRXベクターの文献 （Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003; Saitoh T., J. Biol. Chem. 278 (38): 36005-36012, 2003) の引用を必要とする。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
備考	このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB17223_B0Gkp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: MMLV-pol_F (Pr0352) Region: Pol region, EGFP 5' Sequence file: RDB17223_B0Gka.seq
>17223_17223_B0Gk_MMLV-pol_F_A03_03_ABI24.ab1     1 NNNNNNNNNN NNNNGGNNTC GCAGCTTGGN NNCNCGCCGC CCACGTGAAG GCTGCCGACC    61 CCGGGGGTGG ACCATCCTCT AGACTGCCGG ATCTGCCGCC ACCATGGTGA GCAAGGGCGA   121 GGAGCTGTTC ACCGGGGTGG TGCCCATCCT GGTCGAGCTG GACGGCGACG TAAACGGCCA   181 CAAGTTCAGC GTGTCCGGCG AGGGCGAGGG CGATGCCACC TACGGCAAGC TGACCCTGAA   241 GTTCATCTGC ACCACCGGCA AGCTGCCCGT GCCCTGGCCC ACCCTCGTGA CCACCCTGAC   301 CTACGGCGTG CAGTGCTTCA GCCGCTACCC CGACCACATG AAGCAGCACG ACTTCTTCAA   361 GTCCGCCATG CCCGAAGGCT ACGTCCAGGA GCGCACCATC TTCTTCAAGG ACGACGGCAA   421 CTACAAGACC CGCGCCGAGG TGAAGTTCGA GGGCGACACC CTGGTGAACC GCATCGAGCT   481 GAAGGGCATC GACTTCAAGG AGGACGGCAA CATCCTGGGG CACAAGCTGG AGTACAACTA   541 CAACAGCCAC AACGTCTATA TCATGGCCGA CAAGCAGAAG AACGGCATCA AGGTGAACTT   601 CAAGATCCGC CACAACATCG AGGACGGCAG CGTGCAGCTC GCCGACCACT ACCAGCAGAA   661 CACCCCCATC GGCGACGGCC CCGTGCTGCT GCCCGACAAC CACTACCTGA GCACCCAGTC   721 CGCCCTGANC AAAGACCCCA ACGAGAAGCG CGATCACATG GTCCTGCTGG AGTTCGTGAC   781 CGCCGCCGGN NTCACTCTCG GCATGGACGA NCTGNACAAG TCCGGANTCG GATNCNTGGC   841 GAANAANANG TACGANCTGC TTTTCNAGCT GCNCCTGNTC NGGGGACTCN GGNNTGGNCN   901 NGANCNGCNN CCNTTTTCGN TTTTCNGANG ATGCCTTNNN TACCNNNNTT ATTTNCNCCN 961 NNGGNANTAG NNNTNNNNNT CNAANNNNNN NNANNNNNNN NNN //
Primer: IRES-R (Pr0037) Region: insert, EGFP 3' Sequence file: RDB17223_B0Gkb.seq
>17223_17223_B0Gk_IRES-R_B03_06_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NGTTAGGGGG GGGGGCGGAN NTTACGTAGC GGCCGCGAAT    61 TCACTAGTGA TTTCAGCAGC ACTTGCTCTT CCAGCCCGTC ACGCCTCCTC CACTGCTGAT   121 ATCTACGTTT TCACTGTTGG GTTCTTTTAC AGGGGTCTTT CGGAGGATGT CTTCAGCTAA   181 TGTGAGGAAC GCCTTTTCGA TGTTTATATT TGCTTTTGCA CTAGTCTCAA AAAACCTAAT   241 ACCATGCTCC CTTGCAATCT GTTCTCCTTT GCCTTTCGGT ACAACTCTCT TGTCGTCCAT   301 GTCACACTTG TTCCCTAGTA ACATTCTTTC CACATCTTCA TTGGCATGCT CATCTATGTT   361 TCTAAGCCAC TTGCTGATGT TCTCAAAGCT TTTACCGTTG GTGATGTCAT ACACTAGCAT   421 GATGCCCATT GCTCCTCTGT AGTAGGAGGT TGTGATGGTG TGAAATCGCT CCTGGCCTGC   481 TGTGTCCCAT ATCTGTAGCT TGATCTTCTT TCCTTGTAGT TCCACTGTTT TGATCTTAAA   541 GTCTATTCCT ATGGTGGAAA TAAAGGTGGT ATTGAAGGCA TCGTCCGAAA AACGAAAAAG   601 GACGCAGGTC TTGCCCACTC CCGAGTCCCC GATCAGGAGC AGCTTGAAAA GCAGGTCGTA   661 CGTCTTCTTC GCCATGGATC CGAGTCCGGA CTTGTACAGC TCGTCCATGC CGAGAGTGAT   721 CCCGGCGGCG GTCACGAACT CCAGCAGGAN CATGTGATCG CGCTTCTCGT TGGGGTCTTT   781 GCTCAGGGCG GANTGGGTGC TCAGGTAGTG GTTGTCGGGC AGCAGCACGG GGCCGTCGCC   841 GATGGGGGTG TTCTGCTGGT AGNGGNCGGN GAGCTGCACG CTGCCNNCCN CNNTGTNGTG   901 NCGGATCTTG AAGTTCANNT NNNTGNCNTN CTNNTGNNNN NNGGNNNNNN TATNNNNNTN 961 NNGNCTNNNN NANNNNNNNT NNNNNNNNNN //
Primer: mVenus-M_F (Pr0574) Region: EGFP 3', insert 5' Sequence file: RDB17223_B0Gkc.seq
>17223_17223_B0Gk_mVenus-M_F_C03_09_ABI24.ab1     1 NNNNNNNNNN NNNNGGCNTN AGGTGNNNTT CNNGANCCGC CNCAACATCG AGGACGGCAG    61 CGTGCAGCTC GCCGACCACT ACCAGCAGAA CACCCCCATC GGCGACGGCC CCGTGCTGCT   121 GCCCGACAAC CACTACCTGA GCACCCAGTC CGCCCTGAGC AAAGACCCCA ACGAGAAGCG   181 CGATCACATG GTCCTGCTGG AGTTCGTGAC CGCCGCCGGG ATCACTCTCG GCATGGACGA   241 GCTGTACAAG TCCGGACTCG GATCCATGGC GAAGAAGACG TACGACCTGC TTTTCAAGCT   301 GCTCCTGATC GGGGACTCGG GAGTGGGCAA GACCTGCGTC CTTTTTCGTT TTTCGGACGA   361 TGCCTTCAAT ACCACCTTTA TTTCCACCAT AGGAATAGAC TTTAAGATCA AAACAGTGGA   421 ACTACAAGGA AAGAAGATCA AGCTACAGAT ATGGGACACA GCAGGCCAGG AGCGATTTCA   481 CACCATCACA ACCTCCTACT ACAGAGGAGC AATGGGCATC ATGCTAGTGT ATGACATCAC   541 CAACGGTAAA AGCTTTGAGA ACATCAGCAA GTGGCTTAGA AACATAGATG AGCATGCCAA   601 TGAAGATGTG GAAAGAATGT TACTAGGGAA CAAGTGTGAC ATGGACGACA AGAGAGTTGT   661 ACCGAAAGGC AAAGGAGAAC AGATTGCAAG GGAGCATGGT ATTAGGTTTT TTGAGACTAG   721 TGCAAAAGCA AATATAAACA TCGAAAAGGC GTTCCTCACA TTAGCTGAAG ACATCCTCCG   781 AAAGANCCCT GTAAAAGAAC CCAACAGTGA AAACGTAGAT ATCAGCAGTG GAGGANGCGT   841 GNCGGGCTGG AAGANCAAGT GCNGCTGAAA TCACTAGTGA ATTCGCGGCC GCTACNNAAA   901 TTNCGCCCCC CCCCCCCNAA CGTNACNGGN CGAANCCGNN NNNNNNTNAN NNNCNGNNGN 961 NNGTTTGNCN NTNTNNNNNT TTNNCANNNT ANTNGNCNNN NNTTNGNNNN NNNNNN //
Primer: MMLV-pol_F (Pr0352) Region: Pol region, EGFP 5' Sequence file: RDB17223_B0Gkd.seq
>17223_17223_B0Gk_MMLV-pol_F_A03_03_ABI24.ab1     1 NNNNNNNNNN NNNNGGNNTC GCAGCTTGGN NNCNCGCCGC CCACGTGAAG GCTGCCGACC    61 CCGGGGGTGG ACCATCCTCT AGACTGCCGG ATCTGCCGCC ACCATGGTGA GCAAGGGCGA   121 GGAGCTGTTC ACCGGGGTGG TGCCCATCCT GGTCGAGCTG GACGGCGACG TAAACGGCCA   181 CAAGTTCAGC GTGTCCGGCG AGGGCGAGGG CGATGCCACC TACGGCAAGC TGACCCTGAA   241 GTTCATCTGC ACCACCGGCA AGCTGCCCGT GCCCTGGCCC ACCCTCGTGA CCACCCTGAC   301 CTACGGCGTG CAGTGCTTCA GCCGCTACCC CGACCACATG AAGCAGCACG ACTTCTTCAA   361 GTCCGCCATG CCCGAAGGCT ACGTCCAGGA GCGCACCATC TTCTTCAAGG ACGACGGCAA   421 CTACAAGACC CGCGCCGAGG TGAAGTTCGA GGGCGACACC CTGGTGAACC GCATCGAGCT   481 GAAGGGCATC GACTTCAAGG AGGACGGCAA CATCCTGGGG CACAAGCTGG AGTACAACTA   541 CAACAGCCAC AACGTCTATA TCATGGCCGA CAAGCAGAAG AACGGCATCA AGGTGAACTT   601 CAAGATCCGC CACAACATCG AGGACGGCAG CGTGCAGCTC GCCGACCACT ACCAGCAGAA   661 CACCCCCATC GGCGACGGCC CCGTGCTGCT GCCCGACAAC CACTACCTGA GCACCCAGTC   721 CGCCCTGANC AAAGACCCCA ACGAGAAGCG CGATCACATG GTCCTGCTGG AGTTCGTGAC   781 CGCCGCCGGN NTCACTCTCG GCATGGACGA NCTGNACAAG TCCGGANTCG GATNCNTGGC   841 GAANAANANG TACGANCTGC TTTTCNAGCT GCNCCTGNTC NGGGGACTCN GGNNTGGNCN   901 NGANCNGCNN CCNTTTTCGN TTTTCNGANG ATGCCTTNNN TACCNNNNTT ATTTNCNCCN 961 NNGGNANTAG NNNTNNNNNT CNAANNNNNN NNANNNNNNN NNN //
Primer: IRES-R (Pr0037) Region: insert, EGFP 3' Sequence file: RDB17223_B0Gke.seq
>17223_17223_B0Gk_IRES-R_B03_06_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NGTTAGGGGG GGGGGCGGAN NTTACGTAGC GGCCGCGAAT    61 TCACTAGTGA TTTCAGCAGC ACTTGCTCTT CCAGCCCGTC ACGCCTCCTC CACTGCTGAT   121 ATCTACGTTT TCACTGTTGG GTTCTTTTAC AGGGGTCTTT CGGAGGATGT CTTCAGCTAA   181 TGTGAGGAAC GCCTTTTCGA TGTTTATATT TGCTTTTGCA CTAGTCTCAA AAAACCTAAT   241 ACCATGCTCC CTTGCAATCT GTTCTCCTTT GCCTTTCGGT ACAACTCTCT TGTCGTCCAT   301 GTCACACTTG TTCCCTAGTA ACATTCTTTC CACATCTTCA TTGGCATGCT CATCTATGTT   361 TCTAAGCCAC TTGCTGATGT TCTCAAAGCT TTTACCGTTG GTGATGTCAT ACACTAGCAT   421 GATGCCCATT GCTCCTCTGT AGTAGGAGGT TGTGATGGTG TGAAATCGCT CCTGGCCTGC   481 TGTGTCCCAT ATCTGTAGCT TGATCTTCTT TCCTTGTAGT TCCACTGTTT TGATCTTAAA   541 GTCTATTCCT ATGGTGGAAA TAAAGGTGGT ATTGAAGGCA TCGTCCGAAA AACGAAAAAG   601 GACGCAGGTC TTGCCCACTC CCGAGTCCCC GATCAGGAGC AGCTTGAAAA GCAGGTCGTA   661 CGTCTTCTTC GCCATGGATC CGAGTCCGGA CTTGTACAGC TCGTCCATGC CGAGAGTGAT   721 CCCGGCGGCG GTCACGAACT CCAGCAGGAN CATGTGATCG CGCTTCTCGT TGGGGTCTTT   781 GCTCAGGGCG GANTGGGTGC TCAGGTAGTG GTTGTCGGGC AGCAGCACGG GGCCGTCGCC   841 GATGGGGGTG TTCTGCTGGT AGNGGNCGGN GAGCTGCACG CTGCCNNCCN CNNTGTNGTG   901 NCGGATCTTG AAGTTCANNT NNNTGNCNTN CTNNTGNNNN NNGGNNNNNN TATNNNNNTN 961 NNGNCTNNNN NANNNNNNNT NNNNNNNNNN //
Primer: mVenus-M_F (Pr0574) Region: EGFP 3', insert 5' Sequence file: RDB17223_B0Gkf.seq
>17223_17223_B0Gk_mVenus-M_F_C03_09_ABI24.ab1     1 NNNNNNNNNN NNNNGGCNTN AGGTGNNNTT CNNGANCCGC CNCAACATCG AGGACGGCAG    61 CGTGCAGCTC GCCGACCACT ACCAGCAGAA CACCCCCATC GGCGACGGCC CCGTGCTGCT   121 GCCCGACAAC CACTACCTGA GCACCCAGTC CGCCCTGAGC AAAGACCCCA ACGAGAAGCG   181 CGATCACATG GTCCTGCTGG AGTTCGTGAC CGCCGCCGGG ATCACTCTCG GCATGGACGA   241 GCTGTACAAG TCCGGACTCG GATCCATGGC GAAGAAGACG TACGACCTGC TTTTCAAGCT   301 GCTCCTGATC GGGGACTCGG GAGTGGGCAA GACCTGCGTC CTTTTTCGTT TTTCGGACGA   361 TGCCTTCAAT ACCACCTTTA TTTCCACCAT AGGAATAGAC TTTAAGATCA AAACAGTGGA   421 ACTACAAGGA AAGAAGATCA AGCTACAGAT ATGGGACACA GCAGGCCAGG AGCGATTTCA   481 CACCATCACA ACCTCCTACT ACAGAGGAGC AATGGGCATC ATGCTAGTGT ATGACATCAC   541 CAACGGTAAA AGCTTTGAGA ACATCAGCAA GTGGCTTAGA AACATAGATG AGCATGCCAA   601 TGAAGATGTG GAAAGAATGT TACTAGGGAA CAAGTGTGAC ATGGACGACA AGAGAGTTGT   661 ACCGAAAGGC AAAGGAGAAC AGATTGCAAG GGAGCATGGT ATTAGGTTTT TTGAGACTAG   721 TGCAAAAGCA AATATAAACA TCGAAAAGGC GTTCCTCACA TTAGCTGAAG ACATCCTCCG   781 AAAGANCCCT GTAAAAGAAC CCAACAGTGA AAACGTAGAT ATCAGCAGTG GAGGANGCGT   841 GNCGGGCTGG AAGANCAAGT GCNGCTGAAA TCACTAGTGA ATTCGCGGCC GCTACNNAAA   901 TTNCGCCCCC CCCCCCCNAA CGTNACNGGN CGAANCCGNN NNNNNNTNAN NNNCNGNNGN 961 NNGTTTGNCN NTNTNNNNNT TTNNCANNNT ANTNGNCNNN NNTTNGNNNN NNNNNN //
Primer: Amp_R (Pr0008) Region: AmpR pro, CMV en. Delta U3 5' UTR Sequence file: RDB17223_B0Gkg.seq
>17223_17223_B0Gk_Amp_R_G03_21_ABI24.ab1     1 NNNNNNNNNN NNNNNTGANC ATTTATNNGG GTTATTGTCT CATGAGCGGA TACATATTTG    61 AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC   121 CTGACGTCTA AGAAACCATT ATTATCATGA CATTAACCTA TAAAAATAGG CGTATCACGA   181 GGCCCTTTCG TCTTCACTCG AGGAGCTTGG CCCATTGCAT ACGTTGTATC CATATCATAA   241 TATGTACATT TATATTGGCT CATGTCCAAC ATTACCGCCA TGTTGACATT GATTATTGAC   301 TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA TGGAGTTCCG   361 CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC CCCGCCCATT   421 GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC ATTGACGTCA   481 ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT ATCATATGCC   541 AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT ATGCCCAGTA   601 CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA TCGCTATTAC   661 CATGCTAGAG AACCATCAGA TGTTTCCAGG GTGCCCCAAG GACCTGAAAT GACCCTGTGC   721 CTTATTTGAA CTAACCAATC AGTTCGCTTC TCGCTTCTGT TCGCGCGCTT CTGCTCCCCG   781 AGCTCAATAA AAGAGCCCAC AACCCCTCAC TCGGCGCGCC AGTCCTCCGA TTGANTGANT   841 CGCCCGGNTN NNNGTGTATC CNATAAANCC TCTTGCAGTT GCATCCGANT NGNGGNCTCG   901 CTGTNCCTNG GGANGGTCNN CTCTGANTGN NTNNNTACCC NNCAGCGGGG GNCTTNCATT 961 TNGGGGNNTC GNCCGGGNNC NGNNNANCCN NGCCCNNGNN NNNNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.04.06

GNP_filter3_RDBDEP_html_230405.pl