Please review the QC test results indicated by icon below as well as clone information before placing your order.

Lifeact-DsRed/pCAGEN

Expression vector of Lifeact fused with DsRed1, a versatile marker to visualize F-actin.

Distribution information

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Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kurabayashi N. et al. Development, 145(17), pii: dev162529, 2018.)
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Please review the QC test results indicated by icon below as well as clone information before placing your order.

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB17291_A9Dbp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1 (Pr0164) Region: Lifeact,DsRed1 5' Sequence file: RDB17291_A9Dba.seq
>D06232A3_A9Db_3_pAxCA_F1_G01_19_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNTGCCTTC TTCTTTTTCC NACNGCTCCT GGGCAACGTG    61 CTGGTTATTG TGCTGTCTCA TCATTTTGGC AAAGAATTCC TCGAGCTCAA GCTTCGAATT   121 ATGGGTGTCG CAGATTTGAT CAAGAAATTC GAAAGCATCT CAAAGGAAGA AGGGGATCCA   181 CCGGTCGCCA CCATGGTGCG CTCCTCCAAG AACGTCATCA AGGAGTTCAT GCGCTTCAAG   241 GTGCGCATGG AGGGCACCGT GAACGGCCAC GAGTTCGAGA TCGAGGGCGA GGGCGAGGGC   301 CGCCCCTACG AGGGCCACAA CACCGTGAAG CTGAAGGTGA CCAAGGGCGG CCCCCTGCCC   361 TTCGCCTGGG ACATCCTGTC CCCCCAGTTC CAGTACGGCT CCAAGGTGTA CGTGAAGCAC   421 CCCGCCGACA TCCCCGACTA CAAGAAGCTG TCCTTCCCCG AGGGCTTCAA GTGGGAGCGC   481 GTGATGAACT TCGAGGACGG CGGCGTGGTG ACCGTGACCC AGGACTCCTC CCTGCAGGAC   541 GGCTGCTTCA TCTACAAGGT GAAGTTCATC GGCGTGAACT TCCCCTCCGA CGGCCCCGTA   601 ATGCAGAAGA AGACCATGGG CTGGGAGGCC TCCACCGAGC GCCTGTACCC CCGCGACGGC   661 GTGCTGAAGG GCGAGATCCA CAAGGCCCTG AAGCTGAAGG ACGGCGGCCA CTACCTGGTG   721 GAGTTCAAGT CCATCTACAT GGCCAAGAAG CCCGTGCAGC TGCCCGGCTA CTACTACGTG   781 GACTCCAAGC TGGACATCAC CTCCCACAAC GANGANTACA CCATCGTGGA NCAGTACGAG   841 CGCACCGAGG GCCGCCACCA CCTGTTCCTG TANCGGCCGC ACTCCTCANG NGCANGCTGC   901 CTATCANAAN GNGGTGNNTG GTGTGGNCAA TGNCCTNNNT CACAAATACN ACTGANATNT   961 TTTTCCCNNT GNNNAAANTT ATGGGGNNNN TCATGNANNC NNNNTNNANC NTNNGNNNTN 1021 NNNGNNNNNN NNNN //
Primer: pCAGGS_R1 (Pr0614) Region: beta-globin pA,DsRed1 3' Sequence file: RDB17291_A9Dbb.seq
>D06232A3_A9Db_3_pCAGGS_R1_A02_02_ABI24.ab1     1 NNNNNNNNNN NNNNNNNCNN TTGCNNTGAA AATAAATTTC CTTTATTAGC CAGAAGTCAG    61 ATGCTCAAGG GGCTTCATGA TGTCCCCATA ATTTTTGGCA GAGGGAAAAA GATCTCAGTG   121 GTATTTGTGA GCCAGGGCAT TGGCCACACC AGCCACCACC TTCTGATAGG CAGCCTGCAC   181 CTGAGGAGTG CGGCCGCTAC AGGAACAGGT GGTGGCGGCC CTCGGTGCGC TCGTACTGCT   241 CCACGATGGT GTAGTCCTCG TTGTGGGAGG TGATGTCCAG CTTGGAGTCC ACGTAGTAGT   301 AGCCGGGCAG CTGCACGGGC TTCTTGGCCA TGTAGATGGA CTTGAACTCC ACCAGGTAGT   361 GGCCGCCGTC CTTCAGCTTC AGGGCCTTGT GGATCTCGCC CTTCAGCACG CCGTCGCGGG   421 GGTACAGGCG CTCGGTGGAG GCCTCCCAGC CCATGGTCTT CTTCTGCATT ACGGGGCCGT   481 CGGAGGGGAA GTTCACGCCG ATGAACTTCA CCTTGTAGAT GAAGCAGCCG TCCTGCAGGG   541 AGGAGTCCTG GGTCACGGTC ACCACGCCGC CGTCCTCGAA GTTCATCACG CGCTCCCACT   601 TGAAGCCCTC GGGGAAGGAC AGCTTCTTGT AGTCGGGGAT GTCGGCGGGG TGCTTCACGT   661 ACACCTTGGA GCCGTACTGG AACTGGGGGG ACAGGATGTC CCAGGCGAAG GGCAGGGGGC   721 CGCCCTTGGT CACCTTCAGC TTCACGGTGT TGTGNNCCTC GTANGGGCGG CCCTCGCCCT   781 CGCCCTCGAT CTCGAACTCG TGGCCGTTCA CGGTGCCCTC CNTGCGCACC TTGAAGCGCA   841 TGAACTCCTT GATGANGTTC TTGGANGANC GCACCATGGT GGCGNNCGGN GGNTNCCCTT   901 CTTCCTTTGA NANGCTTTCG AATTTCTTGA TNAANTCTGC NNNNCCNNTA ANTCNAAGCT   961 NNAGCTCNAN GNANNTCTTT NNCNNNANTG NTGANNNAGC NNNNNAANCN GCANNNTNNN 1021 NNNNNNN //
Primer: SV40pro_F (Pr0110) Region: SV40 pro_ori,SV40pA,ori Sequence file: RDB17291_A9Dbc.seq
>D06232A3_A9Db_3_SV40pro_F_H01_22_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNGTGAG GNNGNTTTTT TGGAGGCCTA GGCTTTTGCA    61 AAAAGCTAAC TTGTTTATTG CAGCTTATAA TGGTTACAAA TAAAGCAATA GCATCACAAA   121 TTTCACAAAT AAAGCATTTT TTTCACTGCA TTCTAGTTGT GGTTTGTCCA AACTCATCAA   181 TGTATCTTAT CATGTCTGGA TCCGCTGCAT TAATGAATCG GCCAACGCGC GGGGAGAGGC   241 GGTTTGCGTA TTGGGCGCTC TTCCGCTTCC TCGCTCACTG ACTCGCTGCG CTCGGTCGTT   301 CGGCTGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA   361 GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA   421 AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT   481 CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC   541 CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC   601 GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT   661 TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC   721 CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG   781 CCACTGGCAG CAGCCACTGG TAACAGGANT AGCAGANCGA GGTATGTANG CGGTGCTACA   841 GAGTTCTTGA AGTGGTGGCC TAACTACNGC TACNCTAGAA GAACAGTANT TGGTATCTGC   901 GCTCTGCTGA ANCCNGTTAC NTTNNGNAAA ANAGTTGGTA NCTCTTGATC CGGNNAANNN 961 NNANNGCNNG TANCGGNGGN TTTTTTTGNT TNNNNNCNGC NNNNTNNNNN NNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2024.03.26

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