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CSII-EF-IRES2-Venus_hSOX4 deltaC52

Lentivirus expression vector of human Sox4, truncated TAD (C-teminus, 423 aa - 474 aa).

Catalog number RDB17317
Resource name CSII-EF-IRES2-Venus_hSOX4 deltaC52
Clone info. Lentivirus expression vector of human SRY-box 4 (SOX4), truncated transactivator domain (TAD; C-teminus, 423 aa - 474 aa), EF-1 alpha promoter, Venus. Please refer to Fig. 4a and b of Yoshitomi, H. et al., Nat. Commun., 9 (1): 3762, 2019.
Comment Expression was confirmed by the deposotor with the observation of Venus expression and western blotting.
Vector backbone CSIIR-EF-MCS-IRES2-Venus (plasmid)
Size of vector backbone 10.0 kb
Selectable markers Amp^r.
Gene/insert name human SOX4 cDNA
Depositor|Developer Yoshitomi, Hiroyuki |
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External Database
human SOX4

          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yoshitomi H. et al. Nat. Commun. 9(1), 3762, 2018). The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit organization for a not-for-profit research. For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
          Additional terms and conditions:
          The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer of the lentivirus vector.
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Yoshitomi H. et al. Nat. Commun. 9(1), 3762, 2018)。学術機関の学術研究に限る。営利機関の利用希望者は、事前に利用条件等につき寄託者と合意し、提供承諾を得ること。
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          レンチウイルスベクターについて、本件研究材料を利用した研究結果等を発表する際は、レンチウイルスベクターが故三好浩之博士により開発されたことを明示する。
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          Catalog # Resource name Availability Shipping form Fee (non-profit org.)
          RDB17317 CSII-EF-IRES2-Venus_hSOX4 deltaC52 Under QC test. Please contact us. DNA solution

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.
          Ordering instruction of plasmids [in Japanese] [in English]

          How to cite this biological resource

          Materials & Methods section:

          The CSII-EF-IRES2-Venus_hSOX4 deltaC52 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB17317).

          Reference section:

          Yoshitomi, H., Kobayashi, S., Miyagawa-Hayashino, A., Okahata, A., Doi, K., Nishitani, K., Murata, K., Ito, H., Tsuruyama, T., Haga, H., Matsuda, S., Toguchida, J., Human Sox4 facilitates the development of CXCL13-producing helper T cells in inflammatory environments. Nat. Commun. 9 (1): 3762 (2018). PMID 30232328. [link to RRC of NBRP]

          Further references such as user reports and related articles (go to bottom)


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          QC test results

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          References

          Original, user report and related articles

          original Yoshitomi, H., Human Sox4 facilitates the development of CXCL13-producing helper T cells in inflammatory environments. Nat. Commun. 9 (1): 3762 (2018). PMID 30232328. [link to RRC of NBRP]

          2023.11.23

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