Please review the QC test results indicated by icon below as well as clone information before placing your order.

pDEST131NGFP_BAZ1B

Expression vector of human BAZ1B tagged with GFP at N-terminus.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Ohta S. et al. Mol. Cell Proteomics. 18(2), 169-181, 2019). RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
Remarks	Gateway® Expression clone. Please refer Life Technologies Corporation signs license agreement with RIKEN BRC.

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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Ohta S. et al. Mol. Cell Proteomics. 18(2), 169-181, 2019)。利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び事業活動を行う場合は、寄託者と別途協議を行う。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
備考	Gateway®テクノロジーを用いた発現クローンです。「Gateway®テクノロジーを用いたクローンの提供と寄託のご案内」をご覧ください。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB17334_A9Ehp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: mVenus-M_F () Region: EGFP 3', attB1, insert 5' Sequence file: RDB17334_A9Eha.seq
>D06261A2_A9Eh_3_mVenus-M_F_F01_16_ABI24.ab1     1 NNNNNNNNNN NNNNGGCNTC NNGGNGANTT CNNGANCCGC CNCAACATCG AGGNNGGCAG    61 CGTGCAGCTC GCCGACCACT ACCAGCAGAA CACCCCCATC GGCGACGGCC CCGTGCTGCT   121 GCCCGACAAC CACTACCTGA GCACCCAGTC CGCCCTGAGC AAAGACCCCA ACGAGAAGCG   181 CGATCACATG GTCCTGCTGG AGTTCGTGAC CGCCGCCGGG ATCACTCTCG GCATGGACGA   241 GCTGTACAAG GGTACCACAA GTTTGTACAA AAAAGCAGGC TCCACCATGG GAACCATGGC   301 GCCGCTCCTG GGCCGCAAGC CCTTCCCGCT GGTGAAGCCG TTGCCCGGAG AGGAGCCGCT   361 CTTCACCATC CCGCACACTC AGGAGGCCTT CCGCACCCGG GAAGAGTATG AAGCCCGCTT   421 GGAAAGGTAC AGTGAGCGCA TTTGGACGTG CAAGAGTACT GGAAGCAGTC AGCTAACACA   481 CAAGGAAGCC TGGGAGGAAG AACAGGAAGT TGCTGAGCTT TTGAAGGAGG AGTTTCCTGC   541 CTGGTATGAG AAGCTTGTTC TGGAAATGGT TCACCATAAC ACAGCCTCCT TAGAGAAGTT   601 AGTAGATACT GCTTGGTTGG AGATCATGAC CAAATATGCT GTGGGAGAAG AGTGTGACTT   661 CGAGGTTGGG AAGGAGAAAA TGCTCAAGGT GAAGATTGTG AAGATTCATC CTTTGGAGAA   721 AGTGGATGAA GAGGCCACTG AGAAGAAATC TGATGGTGCC TGTGANTCTC CNTCAAGTGA   781 CANAGAGAAC TCCAGTCAGA TTGCTCANGA NCATCAGAAG ANNGAGANAG TTGTGAAAGA   841 NGATGANNGA AGGANAGAGA GTATTAATGA CAGAGCACGT ANNTCGCCNC GAAANCTTCC   901 TACTTNNTTN AAAAAAAGGA GAANNGAAAT GGGNCTCNNN NNAAATTNNT GNCNNCNCAA 961 NNNTGNNGTN NAACTNNNAA ANNNNNNNNN N //
Primer: BGH_rev2 () Region: BGH poly(A) signal, attB2, insert 3' Sequence file: RDB17334_A9Ehb.seq
>D06261A2_A9Eh_3_BGH_rev2_G01_19_ABI24.ab1     1 NNNNNNNNNN NNNNNNGGGG GNNGGGCNNA NAACAGATGG CTGGCAACTA GAAGGCACAG    61 TCGAGGCTGA TCAGCGGGTT TAAACGGGCC CTCTAGACTA CTTTAGACCA CTTTGTACAA   121 GAAAGCTGGG TCTAGATGCT TCTTCTGTCT TCGTCCCCTG GACTGTCCAA CGGCCTCTGG   181 CTCACTGTCC CCTTCATCTT CAGCAAGCCT ATCAGGAAAC TTCTTGCGCT TCCTGCGGAC   241 ATATGGGTGG CCAGGAAGGT GTTTATGCAA CAGAGCCACT AGACACTGTT CTGTCTTCAC   301 CATGCAGCTT AGCACATGGC TGCCACGGCA GTTGTAAACC TCAGCATTGG TAAACACTTG   361 CTTCATGTCA GTAAGAAACT CCTGCACAGA GCGGTAGCTC CCACAGGAAC ATTTGTTCTG   421 CACTGTCTGA AAGTCCATGG GGTGCGTGAT CACATCATAG TAGTCCTCGG CCTCATCTCT   481 GGTCACAGGC TCCCTGAAGG GCCAGCTGAA GCGGTACTTC ACGATCTTGT GGAGGATCTC   541 TTCACACTTC TGCAGCTCCA GGCTTTGCCT CCGGGAGCTC CGCTTGGTCT GAAGCACCAG   601 CTCATCCACC TCAGCATCAT CCACAGGTGG TGCCTTGGGC TGAGACCTCC TGGTAGAGTG   661 TGGCTTCTTA CCCGGGCGCC GGCCTGACCT TGCTGCAGGG GGGANGACGC TGTGCTTGCC   721 CCGGATGGTC TTTCGAGGTC TCAATCGCAA ACCAGCCACC TCATAATCTT CTTCCTCCTC   781 CTCCTCTTNN NNNTCCTCCT CCTCTTCATC ACTCTCATCA TCTTCACTGT CCTCANAAGC   841 ANANTCTTCA NTATAGTTCC TGCCACGGGN NTTGCGCCNG GCANTANCGG GGCTGGCAAG   901 CTGGGNACTG CNACTCACCA TCNNGNTACN TCATANANGG NCNNNCNCAN ANAAAACNNN   961 NGNANNNNTT NNTTANNNTC NTCANANNNG NTCAANTNNN NNNNCNNNNN CNTTNNNTNN 1021 NNNNANNNTT NNNNNNNNNN //
Primer: CMV-Forward () Region: CMV promoter, EGFP 5' Sequence file: RDB17334_A9Ehc.seq
>D06261A2_A9Eh_3_CMV_Forward_E01_13_ABI24.ab1     1 NNNNNNNNNN NNNNNNTNNN NGCNNTGGGC GGTANGCGTG TACGGTGGGA NGTCTATATA    61 AGCAGAGCTC TCTGGCTAAC TAGAGAACCC ACTGCTTACT GGCTTATCGA AATTAATACG   121 ACTCACTATA GGGAGACCCA AGCTGGCTAG CGTTTAAACT TAAGCTTACG ATGGTGAGCA   181 AGGGCGAGGA GCTGTTCACC GGGGTGGTGC CCATCCTGGT CGAGCTGGAC GGCGACGTAA   241 ACGGCCACAA GTTCAGCGTG TCCGGCGAGG GCGAGGGCGA TGCCACCTAC GGCAAGCTGA   301 CCCTGAAGTT CATCTGCACC ACCGGCAAGC TGCCCGTGCC CTGGCCCACC CTCGTGACCA   361 CCCTGACCTA CGGCGTGCAG TGCTTCAGCC GCTACCCCGA CCACATGAAG CAGCACGACT   421 TCTTCAAGTC CGCCATGCCC GAAGGCTACG TCCAGGAGCG CACCATCTTC TTCAAGGACG   481 ACGGCAACTA CAAGACCCGC GCCGAGGTGA AGTTCGAGGG CGACACCCTG GTGAACCGCA   541 TCGAGCTGAA GGGCATCGAC TTCAAGGAGG ACGGCAACAT CCTGGGGCAC AAGCTGGAGT   601 ACAACTACAA CAGCCACAAC GTCTATATCA TGGCCGACAA GCAGAAGAAC GGCATCAAGG   661 TGAACTTCAA GATCCGCCAC AACATCGAGG ACGGCAGCGT GCAGCTCGCC GACCACTACC   721 AGCAGAACAC CCCCATCGGC GACNGCCCCG TGCTGCTGCC CGACAACCAC TACCTGANCA   781 CCCAGTCCGC CNTGANCANA GNNCCCAACG AGAAGCGCGA TCACATGGTC CTGCTGGANT   841 TCGNGACCGC CGCCNGGNTC ACTCTCGGCA TGGNNGANCT GNANNNNNNT ANCACNNGTT   901 TGTANNAAAA ANCNNNNTCC NCCNNNGGNA NCCANGGNNC NNCNNCNGGG NCNNNNAGCC 961 NNTNCCCNCN NNNNNNNNCN NNTNNNCNNG NANNNGNNNN CNNNNNNNNN N //
Primer: SV40polyA-R2 () Region: SV40 poly(A) signal, NeoR/KanR Sequence file: RDB17334_A9Ehd.seq
>D06261A2_A9Eh_3_SV40polyA-R2_H01_22_ABI24.ab1     1 NNNNNNNNNC NNNNNNANNN NNGTTGGGGT GGGCGAANAA CTCCNGCATG AGNTCCCCGC    61 GCTGGANGNN CATCCAGCCG GCGTCCCGGA AAACGATTCC GAAGCCCAAC CTTTCATAGA   121 AGGCGGCGGT GGAATCGAAA TCTCGTGATG GCAGGTTGGG CGTCGCTTGG TCGGTCATTT   181 CGAACCCCAG AGTCCCGCTC AGAAGAACTC GTCAAGAAGG CGATAGAAGG CGATGCGCTG   241 CGAATCGGGA GCGGCGATAC CGTAAAGCAC GAGGAAGCGG TCAGCCCATT CGCCGCCAAG   301 CTCTTCAGCA ATATCACGGG TAGCCAACGC TATGTCCTGA TAGCGGTCCG CCACACCCAG   361 CCGGCCACAG TCGATGAATC CAGAAAAGCG GCCATTTTCC ACCATGATAT TCGGCAAGCA   421 GGCATCGCCA TGGGTCACGA CGAGATCCTC GCCGTCGGGC ATGCGCGCCT TGAGCCTGGC   481 GAACAGTTCG GCTGGCGCGA GCCCCTGATG CTCTTCGTCC AGATCATCCT GATCGACAAG   541 ACCGGCTTCC ATCCGAGTAC GTGCTCGCTC GATGCGATGT TTCGCTTGGT GGTCGAATGG   601 GCAGGTAGCC GGATCAAGCG TATGCAGCCG CCGCATTGCA TCAGCCATGA TGGATACTTT   661 CTCGGCAGGA GCAAGGTGAG ATGACAGGAG ATCCTGCCCC GGCACTTCGC CCAATAGCAG   721 CCAGTCCCTT CCCGCTTCAG TGACAACGTC GAGCACAGCT GCGCAAGGAA CGCCCGTCGN   781 GGCCAGCCAC GATAGCCGCG CTGCCTCGTC CTGCAGTTCA TTCNNGGCAC CGGANANGTC   841 GGTCTTGANA AAAAGAANCG GGCGCCCCTG CGCTNANAGC CGGAACACGG CNGCNTCANA   901 NCAGCCGANT GTCNGNTGTG CCCAGNCATA NCCNAANNNC CNCTNNNCCN NNNNNNNCNG   961 NANNANCNNN NNNGNNNTNN NNNNTNNNTN NNNNNNNGNN NANNNNNNCN NNNNNCNNNN 1021 NNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.04.06

GNP_filter3_RDBDEP_html_230405.pl