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pMRX-puro-EGFP-Rab6A

Retroviral expression vector of mouse Rab6A.

Catalog number RDB17361
Resource name pMRX-puro-EGFP-Rab6A
Clone info. Retroviral expression vector of mouse Rab6A tagged with EGFP at N-terminus.
Vector backbone pMRX-puro-EGFP (plasmid)
Selectable markers Amp^r (E. coli), puro^r (mammalian cells).
Gene/insert name mouse Rab6a cDNA
Reference of insert sequence AB232596 (DDBJ accession number)
Depositor|Developer Fukuda, Mitsunori |
Other clones in our bank

External Database
mouse Rab6a

          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kuroda, T. S. et al. J. Biol. Chem. 277, 9212-9218, 2002); (Homma, Y. et al. J. Cell Biol., in press (doi: 10.1083/jcb.201810134), 2019)
          Additional terms and conditions:
          Regarding pMRX vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literatures for pMX and pMRX vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003; Saitoh T., J. Biol. Chem. 278 (38): 36005-36012, 2003) is requested.
          Ordering Please visit Information of Request for Distribution.[link] 
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          Remarks Remember that you will be working with samples containing infectious virus.
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Kuroda, T. S. et al. J. Biol. Chem. 277, 9212-9218, 2002); (Homma, Y. et al. J. Cell Biol., in press (doi: 10.1083/jcb.201810134), 2019)。
          付加的提供条件:
          pMRXベクターについて:利用者は、研究成果の公表にあたって pMXならびにpMRXベクターの文献 (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003; Saitoh T., J. Biol. Chem. 278 (38): 36005-36012, 2003) の引用を必要とする。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB17361 pMRX-puro-EGFP-Rab6A DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Original reference

          original Homma, Y., Comprehensive knockout analysis of the Rab family GTPases in epithelial cells. J. Cell Biol. 218 (6): 2035-2050 (2019). PMID 31072826.

          Further references such as user reports and related articles (go to bottom)

          Featured content


          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB17361_A9Fap1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: gag-M_F ()
          Region: gag,pol region,EGFP
          Sequence file: RDB17361_A9Faa.seq check
          >D06377A5_A9Fa_1_gag-M_F_A03_03_ABI24.ab1
              1 NNNNNNNNNN NNTNNNNNNN CCTAAGCCTC CGCCTCCTCT TCCTCCNTCC GCCCCGTCTC
             61 TCCCCCTTGA ACCTCCTCGT TCGACCCCGC CTCGATCCTC CCTTTATCCA GCCCTCACTC
            121 CTTCTCTAGG CGCCCCCATA TGGCCATATG AGATCTTATA TGGGGCACCC CCGCCCCTTG
            181 TAAACTTCCC TGACCCTGAC ATGACAAGAG TTACTAACAG CCCCTCTCTC CAAGCTCACT
            241 TACAGGCTCT CTACTTAGTC CAGCACGAAG TCTGGAGACC TCTGGCGGCA GCCTACCAAG
            301 AACAACTGGA CCGACCGGTG GTACCTCACC CTTACCGAGT CGGCGACACA GTGTGGGTCC
            361 GCCGACACCA GACTAAGAAC CTAGAACCTC GCTGGAAAGG ACCTTACACA GTCCTGCTGA
            421 CCACCCCCAC CGCCCTCAAA GTAGACGGCA TCGCAGCTTG GATACACGCC GCCCACGTGA
            481 AGGCTGCCGA CCCCGGGGGT GGACCATCCT CTAGACTGCC GGATCTGCCG CCACCATGGT
            541 GAGCAAGGGC GAGGAGCTGT TCACCGGGGT GGTGCCCATC CTGGTCGAGC TGGACGGCGA
            601 CGTAAACGGC CACAAGTTCA GCGTGTCCGG CGAGGGCGAG GGCGATGCCA CCTACGGCAA
            661 GCTGACCCTG AAGTTCATCT GCACCACCGG CAAGCTGCCC GTGCCCTGGC CCACCCTCGT
            721 GANCACCCTG ACCTACGGCG TGCAGTGCTT CAGCCGCTAC CCCGACCACA TGAAGCAGCA
            781 CGACTTCTTC AAGTCCGCCN TGCCCGANNG CTACGTCCAG GANCGCACCA TCTTCTTCAA
            841 NGACGANNGC AACTACNAGA NCCGCGCCGA NGTGAAGTTC GAGGGCGANA CCCNNGNGAA
            901 NCGCATCGNN CTGAANNGNN ATCGACTNNN NNNAGGANNG NAACNTCCTG GGGNCANANG
            961 CNGGANTANN ACTACNNNNG NCNNNANNNC TATATCNTNG NNNNANNNGC ANAANAANNN
          1021 NNTNNANNNN NNNNNN
          //
          Primer: IRES-R ()
          Region: IRES,insert,EGFP
          Sequence file: RDB17361_A9Fab.seq check
          >D06377A5_A9Fa_1_IRES-R_B03_06_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNGTTAGGG GGGGGGGCGG ANTTTACGTA GCGGCCGCGA
             61 ATTCACTAGT GATTTTAGCA GGAACAGCCG CCTTCATTGA CTGGTTGCTC CTGAGGCTTT
            121 TCCAGTTTTA TGTCAATCAT GTCTTCTCTG CTTCTGTCCT GTGTGCTTTC CATTCCAGGT
            181 AAAGCTGCAG CAACACGCCG GAAAAGCTGC TTTACATTGT ATCCTGCTTT TGCACTGGTT
            241 TCAATAAACA TAACATTCAG CTCTTTGGCT TTCCTCTCTC CCTCCTCAAT TGACACTTGC
            301 CTCTTATCGG CAAGATCTGT TTTATTTCCT ACTAGCATGA TGATGACATC ACTTCCTCTC
            361 TCTGTTCTGA CATCATCAAT CCATTTTGTA GTTTGCTGGA ATGAGTTAAC ATTTGTGATA
            421 TCATAAACAA CAACTGCCAC AGTGGAGTCT CGAATGTAGC TAGGAATCAA GCTCCTGAAC
            481 CGCTCTTGAC CTGCTGTGTC CCATAATTGC AATCGCACGG TTCTATCCTC CAAGTACATT
            541 GTTTTTGATA AGAAGTCAAT GCCAATTGTT GCCTGATAGG TGTTGTCAAA ACTGTCATAC
            601 ATGAATCGGG TGATCAAGGA CGTCTTTCCA ACGCTCTGCT CTCCCAGGAA CACCAGCTTG
            661 AATTTCCTCA GCGGATTCCC GAAGTCTCCG CCCGCGGACA TGGATCCGAG TCCGGACTTG
            721 TACAGCTCGT CCATGCCGAG AGTGATCCCG GCGGCGGTCA CGAACTCCAG CAGGANCATG
            781 TGATCGCGCT TCTCGTTGGG GTCTTTGCTC ANGGCGGANT GGGTGCTCAN GTAGTGGTTG
            841 TCGGGCAGCA GCACGGGGCC GTCGCCNANG GGGGTGNTCT GCTGGTAGTG GNCGGCGANC
            901 TGCACGCTGC CGNCCTCGAT GTTGTGNNGN NNCTTGNAGT TCACCTNNNT GCCNTNCNNC
          961 TGCTNNNNNN NNNN
          //
          Primer: EGFP-C2 ()
          Region: EGFP,insert
          Sequence file: RDB17361_A9Fac.seq check
          >D06377A5_A9Fa_1_EGFP-C2_C03_09_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNGNN CNCNTGGTCC TGCTGGAGTT CGTGANCGCC GCCGGGNTCA
             61 CTCTCGGCAT GGNCNAGCTG TACAAGTCCG GACTCGGATC CATGTCCGCG GGCGGAGACT
            121 TCGGGAATCC GCTGAGGAAA TTCAAGCTGG TGTTCCTGGG AGAGCAGAGC GTTGGAAAGA
            181 CGTCCTTGAT CACCCGATTC ATGTATGACA GTTTTGACAA CACCTATCAG GCAACAATTG
            241 GCATTGACTT CTTATCAAAA ACAATGTACT TGGAGGATAG AACCGTGCGA TTGCAATTAT
            301 GGGACACAGC AGGTCAAGAG CGGTTCAGGA GCTTGATTCC TAGCTACATT CGAGACTCCA
            361 CTGTGGCAGT TGTTGTTTAT GATATCACAA ATGTTAACTC ATTCCAGCAA ACTACAAAAT
            421 GGATTGATGA TGTCAGAACA GAGAGAGGAA GTGATGTCAT CATCATGCTA GTAGGAAATA
            481 AAACAGATCT TGCCGATAAG AGGCAAGTGT CAATTGAGGA GGGAGAGAGG AAAGCCAAAG
            541 AGCTGAATGT TATGTTTATT GAAACCAGTG CAAAAGCAGG ATACAATGTA AAGCAGCTTT
            601 TCCGGCGTGT TGCTGCAGCT TTACCTGGAA TGGAAAGCAC ACAGGACAGA AGCAGAGAAG
            661 ACATGATTGA CATAAAACTG GAAAAGCCTC ANGAGCAACC AGTCAATGAA GGCGGCTGTT
            721 CCTGCTAAAA TCACTAGTGA ATTCGCGGCC GCTACGTAAA TTCCGCCCCC CCCCCTAACG
            781 TTACTGGCCG AAGCCGCTTG GAATAANGNC GGTGTGCGTT TGTCTATATG TTATTTTCCA
            841 CCATATTGCC GTCTTTTGGC AATGTGAGGG CCCGGAAANC NGNNCCNGNC TTCTTGANNA
            901 NCATNNCTAG GGGTCTTTCC CCNNNCGCCN AANGNAANGN NANNNTCNGT TGANNGNCNN
          961 GNANNNNNNC AGTNNCNTCN GNNANNCNTC NTNNNNNANN ANCNNNNNNN NNN
          //
          Primer: EGFP-N_rev ()
          Region: EGFP,insert
          Sequence file: RDB17361_A9Fad.seq check
          >D06377A5_A9Fa_1_EGFP-N_rev_F10_06_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNGGCG NNGCCNCCTA CGGNAAGCTG
             61 ACCCTGAAGT TCATCTGCAC CACCGGCAAG CTGCCCGTGC CCTGGCCCAC CCTCGTGACC
            121 ACCCTGACCT ACGGCGTGCA GTGCTTCAGC CGCTACCCCG ACCACATGAA GCAGCACGAC
            181 TTCTTCAAGT CCGCCATGCC CGAAGGCTAC GTCCAGGAGC GCACCATCTT CTTCAAGGAC
            241 GACGGCAACT ACAAGACCCG CGCCGAGGTG AAGTTCGAGG GCGACACCCT GGTGAACCGC
            301 ATCGAGCTGA AGGGCATCGA CTTCAAGGAG GACGGCAACA TCCTGGGGCA CAAGCTGGAG
            361 TACAACTACA ACAGCCACAA CGTCTATATC ATGGCCGACA AGCAGAAGAA CGGCATCAAG
            421 GTGAACTTCA AGATCCGCCA CAACATCGAG GACGGCAGCG TGCAGCTCGC CGACCACTAC
            481 CAGCAGAACA CCCCCATCGG CGACGGCCCC GTGCTGCTGC CCGACAACCA CTACCTGAGC
            541 ACCCAGTCCG CCCTGAGCAA AGACCCCAAC GAGAAGCGCG ATCACATGGT CCTGCTGGAG
            601 TTCGTGACCG CCGCCGGGAT CACTCTCGGC ATGGACGAGC TGTACAAGTC CGGACTCGGA
            661 TCCATGTCCG CGGGCGGAGA CTTCGGGAAT CCGCTGAGGA AATTCAAGCT GGTGTTCCTG
            721 GGAGAGCANA GCGTTGGAAA GACGTCCTTG ATCACCCGAT TCATGTATGA CAGTTTTGAC
            781 AACACCTATC NNGCAACAAN TGGCATTGAC TTCTTATCNA AAACNATGTA CTTGGANGAT
            841 AGAANNCGTG CNATTGCNAN TTATGGGACN CAGCNNNCAN GANCGGTTCA NGANCTTGAN
            901 NNNTAGCNNN NNTTCNAGAN TCCACNGTGG NCAGTTGTTG NTTTATGNAT ANNCNNCNAA
            961 NNGNTNNANT NNNTNNCNNG NCNAACNNNN AAANNNGNNN TNGNNNGNNN GNNCNNANNN
          1021 NNNANNNNNN GNAANNNNNT NNNNATNNNN NNNNTNNNNN NNNNN
          //
          Primer: HCMVIEE_F2 ()
          Region: CMV en,delta U3 5'LTR,MMLV psi
          Sequence file: RDB17361_A9Fae.seq check
          >D06377A5_A9Fa_1_HCMVIEE_F2_H03_08_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNCNNNNNNN NNCTTACGGT AAATGGCCCG CCTGGCTGNN
             61 CGCCCAACGA CCCCCGCCCA TTGNCGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA
            121 TAGGGACTTT CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG
            181 TACATCAAGT GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC
            241 CCGCCTGGCA TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT
            301 ACGTATTAGT CATCGCTATT ACCATGCTAG AGAACCATCA GATGTTTCCA GGGTGCCCCA
            361 AGGACCTGAA ATGACCCTGT GCCTTATTTG AACTAACCAA TCAGTTCGCT TCTCGCTTCT
            421 GTTCGCGCGC TTCTGCTCCC CGAGCTCAAT AAAAGAGCCC ACAACCCCTC ACTCGGCGCG
            481 CCAGTCCTCC GATTGACTGA GTCGCCCGGG TACCCGTGTA TCCAATAAAC CCTCTTGCAG
            541 TTGCATCCGA CTTGTGGTCT CGCTGTTCCT TGGGAGGGTC TCCTCTGAGT GATTGACTAC
            601 CCGTCAGCGG GGGTCTTTCA TTTGGGGGCT CGTCCGGGAT CGGGAGACCC CTGCCCAGGG
            661 ACCACCGACC CACCACCGGG AGGTAAGCTG GCCAGCAACT TATCTGTGTC TGTCCGATTG
            721 TCTAGTGTCT ATGACTGATT TTATGCGCCT GCGTCGGTAC TAGTTAGCTA ACTAGCTCTG
            781 TATCTGGCGG ANCCNTGGTG GAACTGANGA GTTCNGAACA CCCNGNNGCA ACCCNGGGAG
            841 ANGTCCCNNG GACTTCNGGG GGCCGTTTTT GTGNNCCGAN CTGANTCCNA AAATCCCNNN
            901 TCGNTTTNNG ACTCTTTGGT GCACCCCCCC TTNNANGAGG GANNNGTGGN NNTGGNAGGA
            961 NANNANNNNC TNAAANANNT NCCCGNNNNN NNNNTGAATT TTTNNNNTTN NNNNTNNGNN
          1021 NNNNNAANNN CNNNNNNNNN N
          //
          Primer: pOTB7_R ()
          Region: LTR,PuroR
          Sequence file: RDB17361_A9Faf.seq check
          >D06377A5_A9Fa_1_pOTB7_R_F03_18_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNT NNNNNNNNNN NNNNNNNNNN NNNNNNNGAN ACNTGCTGCA
             61 TGTGAAAGAC CCCCGCTGAC GGGTAGTCAA TCACTCAGAG GAGACCCTCC CAAGGAACAG
            121 CGAGACCACA AGTCGGATGC AACTGCAAGA GGGTTTATTG GATACACGGG TACCCGGGCG
            181 ACTCAGTCAA TCGGAGGACT GGCGCCCCGA GTGAGGGGTT GTGGGCTCTT TTATTGAGCT
            241 CGGGGAGCAG AAGCGCGCGA ACAGAAGCGA GAAGCGAACT GATTGGTTAG TTCAAATAAG
            301 GCACAGGGTC ATTTCAGGTC CTTGGGGCAC CCTGGAAACA TCTGATGGTT CTCTAGAAAC
            361 TGCTGAGGGC TGGACCGCAT CTGGGGACCA TCTGTTCTTG GCCCTGAGCC GGGGCAGGAA
            421 CTGCTTACCA CAGATATCCT GTTTGGCCCA TATTCAGCTG TTCCATCTGT TCTTGGCCCT
            481 GAGCCGGGGC AGGAACTGCT TACCACAGAT ATCCTGTTTG GCCCATATTC AGCTGTTCCA
            541 TCTGTTCCTG ACCTTGATCT GAACTTCTCT ATTCTCAGTT ATGTATTTTT CCATGCCTTG
            601 CAAAATGGCG TTACTTAAGC TAGCTTGCCA AACCTACAGG TGGGGTCTTT CATTCCCCCC
            661 TTTTTCTGGA GACTAAATAA AATCTTTTAT TTTATCGTCG AGCCATGGGG TCGTGCGCTC
            721 CTTTCGGTCG GGCGCTGCGG GTCGTGGGGC GGGCGTCANG CACCGGGCTT GCGGGTCATG
            781 CACCAGGTGC GCGGTCCTTC GGGCACCTCG ACGTCNGCGG TGACGGTGAA NCCGANCCGC
            841 TCGTANAANG GGANGTTGCG GGGCGCGGNN GTCTCCAGGA ANGNGGGCAC CCCGGCGCGC
            901 TCNGNCGCCT CCNCTCCNGG GANCNNGANN GCNCTGNCCA NANCCNNGCC CNGGTGGNNG
          961 GGCGANANNC NNANNNGGNC AGNANNCNCN CNGGNTNCNT NNNNCNNNNN NNNNN
          //
          Primer: IRES-F ()
          Region: IRES,PuroR
          Sequence file: RDB17361_A9Fag.seq check
          >D06377A5_A9Fa_1_IRES-F_G03_21_ABI24.ab1
              1 NNNNNNNNNN NNNNTTNNGT CGAGGTTNNN AAAACGTCTA GGCCCCCCGA ACCACGGGGN
             61 CGTGGTTTTC CTTTGAAAAA CACGATGATA ATATGGCCAC AACCATGACC GAGTACAAGC
            121 CCACGGTGCG CCTCGCCACC CGCGACGACG TCCCCCGGGC CGTACGCACC CTCGCCGCCG
            181 CGTTCGCCGA CTACCCCGCC ACGCGCCACA CCGTCGACCC GGACCGCCAC ATCGAGCGGG
            241 TCACCGAGCT GCAAGAACTC TTCCTCACGC GCGTCGGGCT CGACATCGGC AAGGTGTGGG
            301 TCGCGGACGA CGGCGCCGCG GTGGCGGTCT GGACCACGCC GGAGAGCGTC GAAGCGGGGG
            361 CGGTGTTCGC CGAGATCGGC CCGCGCATGG CCGAGTTGAG CGGTTCCCGG CTGGCCGCGC
            421 AGCAACAGAT GGAAGGCCTC CTGGCGCCGC ACCGGCCCAA GGAGCCCGCG TGGTTCCTGG
            481 CCACCGTCGG CGTCTCGCCC GACCACCAGG GCAAGGGTCT GGGCAGCGCC GTCGTGCTCC
            541 CCGGAGTGGA GGCGGCCGAG CGCGCCGGGG TGCCCGCCTT CCTGGAGACC TCCGCGCCCC
            601 GCAACCTCCC CTTCTACGAG CGGCTCGGCT TCACCGTCAC CGCCGACGTC GAGGTGCCCG
            661 AAGGACCGCG CACCTGGTGC ATGACCCGCA AGCCCGGTGC CTGACGCCCG CCCCACGACC
            721 CGCAGCGCCC GACCGAAAGG AGCGCACGAC CCCATGGCTC GACGATAAAA TAAAAGANTT
            781 TATTTAGTCT CCAGANAAAN GGGGGAATGA AAGANCCCAC CTGTANGTTT GGNAAGCTAG
            841 CTTAAGTAAC GCCATTTTGC AAGGNATGGA AAAATACATA ACTGANAATA GAGAANTTCA
            901 NATNNANGGT CNGGAACAGA TGGNACAGCT NNANNTGGGC CNAACAGGNT NTCTGTGGNA
            961 ANCAGTTNNN GNCCCGGNTC NNNNCNNNNN NCAGATGNNN NNNCNNNNNA TNGNNCNAAA
          1021 CNNNNNNNNN N
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Homma, Y., Comprehensive knockout analysis of the Rab family GTPases in epithelial cells. J. Cell Biol. 218 (6): 2035-2050 (2019). PMID 31072826.
          reference Kuroda, T.S., The Slp homology domain of synaptotagmin-like proteins 1-4 and Slac2 functions as a novel Rab27A binding domain. J. Biol. Chem., 277, 9212-9218 (2002). PMID 11773082.

          2023.04.06

          GNP_filter3_RDBDEP_html_230405.pl