Please review the QC test results indicated by icon below as well as clone information before placing your order.

pMRX-bsr-ss-EGFP-FM4

Retroviral expression vector of secretory form of EGFP fused with a conditional aggregation domain.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Hirano, S. et al. Autophagy 12, 312-326 (FM4), 2016); (Homma, Y. et al. J. Cell Biol., in press (doi: 10.1083/jcb.201810134), 2019) Additional terms and conditions: Regarding pMRX vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literatures for pMX and pMRX vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003; Saitoh T., J. Biol. Chem. 278 (38): 36005-36012, 2003) is requested.
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
Remarks	Remember that you will be working with samples containing infectious virus.

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Hirano, S. et al. Autophagy 12, 312-326 (FM4), 2016); (Homma, Y. et al. J. Cell Biol., in press (doi: 10.1083/jcb.201810134), 2019)。 付加的提供条件： pMRXベクターについて：利用者は、研究成果の公表にあたって pMXならびにpMRXベクターの文献 （Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003; Saitoh T., J. Biol. Chem. 278 (38): 36005-36012, 2003) の引用を必要とする。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
備考	このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB17364_A9Lgp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: MMLV-pol_F (Pr0352) Region: pol region, SS, EGFP 5' Sequence file: RDB17364_A9Lga.seq
>D06377A8_A9Lg_1_MMLV-pol_F_A08_02_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNG GNATCGCAGC TTGGNNACNC GCCGCCCACG TGAAGGCTGC    61 CGACCCCGGG GGTGGACCAT CCTCTAGACT GCCGGATCTG CCACCATGCT CCTTTCGGTG   121 CCGCTCCTGC TTGGCCTCCT CGGCCTGGCC GCCGCAAGAT CCATGGTGAG CAAGGGCGAG   181 GAGCTGTTCA CCGGGGTGGT GCCCATCCTG GTCGAGCTGG ACGGCGACGT AAACGGCCAC   241 AAGTTCAGCG TGTCCGGCGA GGGCGAGGGC GATGCCACCT ACGGCAAGCT GACCCTGAAG   301 TTCATCTGCA CCACCGGCAA GCTGCCCGTG CCCTGGCCCA CCCTCGTGAC CACCCTGACC   361 TACGGCGTGC AGTGCTTCAG CCGCTACCCC GACCACATGA AGCAGCACGA CTTCTTCAAG   421 TCCGCCATGC CCGAAGGCTA CGTCCAGGAG CGCACCATCT TCTTCAAGGA CGACGGCAAC   481 TACAAGACCC GCGCCGAGGT GAAGTTCGAG GGCGACACCC TGGTGAACCG CATCGAGCTG   541 AAGGGCATCG ACTTCAAGGA GGACGGCAAC ATCCTGGGGC ACAAGCTGGA GTACAACTAC   601 AACAGCCACA ACGTCTATAT CATGGCCGAC AAGCAGAAGA ACGGCATCAA GGTGAACTTC   661 AAGATCCGCC ACAACATCGA GGACGGCAGC GTGCAGCTCG CCGACCACTA CCAGCAGAAC   721 ACCCCCATCG GCGACGGCCC CGTGCTGCTG CCCGACAACC ACTACCTGAN CACCCAGTCC   781 GCCCTGANCA AAGACCCCAA CGAGAAGCGC GATCACATGG TCCTGCTGGA NTTCGTGACC   841 GCCGCCGGGN TCACTCTCGG CATGGACGAN CTGTACAANG GATCNNGANN GCANGNGGAG   901 ANNNTCTCNN CTGGANNNGG GNGCACCNTN NCNNAAGCGC GGNCAGANNN GCNNNNNNCA 961 CTACNCGGGN NNGNNNNNNN NNGGNAAGAA ANNNNNNNNN N //
Primer: EGFP-C2 (Pr0402) Region: EGFP 3', FM(1), FM(2), FM(3) Sequence file: RDB17364_A9Lgb.seq
>D06377A8_A9Lg_1_EGFP-C2_F08_17_ABI24.ab1     1 NNNNNNNNNN NNNNNCNNGN NCNCNTGGTC CTGCTGGANT TCGTGNCCGC CGCCGGGNTC    61 ACTCTCGGCA TGGANGAGCT GTACAAGGGA TCCGGAGTGC AGGTGGAGAC CATCTCTCCT   121 GGAGACGGGC GCACCTTCCC AAAGCGCGGC CAGACCTGCG TGGTGCACTA CACGGGGATG   181 CTTGAAGATG GAAAGAAAAT GGATTCCTCT CGGGACAGAA ACAAGCCTTT TAAGTTTACA   241 CTAGGCAAGC AGGAGGTGAT CCGAGGCTGG GAGGAAGGGG TAGCCCAGAT GAGTGTGGGT   301 CAGAGAGCCA AACTGATAAT CTCCTCAGAC TATGCCTATG GAGCCACCGG GCACCCAGGC   361 ATCATCCCAC CACATGCCAC TCTTGTTTTT GATGTGGAGC TTCTAAAACT GGAAGGAAGA   421 TCCGGAGTGC AGGTGGAGAC CATCTCTCCT GGAGACGGGC GCACCTTCCC AAAGCGCGGC   481 CAGACCTGCG TGGTGCACTA CACGGGGATG CTTGAAGATG GAAAGAAAAT GGATTCCTCT   541 CGGGACAGAA ACAAGCCTTT TAAGTTTACA CTAGGCAAGC AGGAGGTGAT CCGAGGCTGG   601 GAGGAAGGGG TAGCCCAGAT GAGTGTGGGT CAGAGAGCCA AACTGATAAT CTCCTCAGAC   661 TATGCCTATG GAGCCACCGG GCACCCAGGC ATCATCCCAC CACATGCCAC TCTTGTTTTT   721 GATGTGGAGC TTCTAAAACT GGAAGGAAGA TCCGGANTGC AGGTGGAGAN CATCTCTCCT   781 GGAGACGGGC GCACCTTCCC NAAGCGCGGC CAGANCTGCG TGGTGCACTA CACGGGGNNG   841 CTTGAAGATG GAAAGAAAAT GGATTCCTCT CGGGACAGAA ACAAGCCTTT TAAGTTTACA   901 CTANGNNAGC AGGANGNGAT NCNANGNTGG GANGNNNGGG NANCCCAGAT GANTGNGGNN   961 CANANANCCA AANTGANNNN CTNCNNCNNN NTATGNNNNT GGNNNNNNNN GNNNNNNNNN 1021 N //
Primer: IRES-R (Pr0037) Region: FM(4) Sequence file: RDB17364_A9Lgc.seq
>D06377A8_A9Lg_1_IRES-R_B08_05_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN ANGTNAGGGG GGGGGGGCGG AATTGNTCCC GCTAGAGGGA    61 AACCGTTGCT AGCTTAAGTT AGTTAACAAT TGTTACCGCG GCCGCGAATT CACTAGTGAT   121 TCTACGTCGA CCCTCCTTCC AGTTTTAGAA GCTCCACATC AAAAACAAGA GTGGCATGTG   181 GTGGGATGAT GCCTGGGTGC CCGGTGGCTC CATAGGCATA GTCTGAGGAG ATTATCAGTT   241 TGGCTCTCTG ACCCACACTC ATCTGGGCTA CCCCTTCCTC CCAGCCTCGG ATCACCTCCT   301 GCTTGCCTAG NGTAAACTTA AAAGGCTTGT TTCTGTCCCG AGAGGAATCC ATTTTCTTTC   361 CATCTTCAAG CATCCCCGTG TAGTGCACCA CGCAGGTCTG GCCGCGCTTT GGGAAGGTGC   421 GCCCGTCTCC AGGANANATG GTCTCCACCT GCACTCCGGA TCTTCCTTCC AGTTTTANAA   481 GCTCCACATC AAAAACAANA GTGGCATGTG GTGGGATGAT GCCTGGGTGC CCGGTGGCTC   541 CATAGGCATA GTCTGAGGAG ATTATCAGTT TGGCTCTCTG ACCCACACTC ATCTGGGCTA   601 CCCCTTCCTC CCANCCTCGG ATCACCTCCT GCTTGCCTAG TGTAAACTTA AAAGGCTTGT   661 TTCTGTCCCG AGAGGAATCC ATTTTCTTTC CATCTTCAAG CATCCCCGTG TAGTGCACCA   721 CGCAGGTCTG GCCGCGCTTT GGGAANGTGC GCCCGTCTCC AGGANAGANG GTCTCCACCT   781 GCACTCCGGA TCTTCCTTCC AGTTTTANAA GCTCCACATC AAAAACAAGA NNGGCATGTG   841 NNGGGNNGAN GCCNGGGNGC CCGGNGGCTC CNTAGGCNTA GNCNGNNGAN ATTATCAGTT   901 TGNNTCTCTG ANCCACANTC NTCNGGGCTA CCCNNNCNNN CCNNNNNCNN NTNNNNNNCN   961 GCTTGCNNAN NGNAAACTNN AAANNNNNTN NNTNNTGTCC NNNNNNGNNN NNNNNTTNNN 1021 TTNNNNNNNN NN //
Primer: IRES-F (Pr0036) Region: IRES, bsr, 3' LTR Sequence file: RDB17364_A9Lgd.seq
>D06377A8_A9Lg_1_IRES-F_C08_08_ABI24.ab1     1 NNNNNNNNNN NNNTNNGTCG AGGNNNNNAA ACGTCTAGGC CCCCCGAACC ACGGGGANGT    61 GGTTTTCCTT TGAAAAACAC GATAATACCA TGGTCATGAA AACATTTAAC ATTTCTCAAC   121 AAGATCTAGA ATTAGTAGAA GTAGCGACAG AGAAGATTAC AATGCTTTAT GAGGATAATA   181 AACATCATGT GGGAGCGGCA ATTCGTACGA AAACAGGAGA AATCATTTCG GCAGTACATA   241 TTGAAGCGTA TATAGGACGA GTAACTGTTT GTGCAGAAGC CATTGCGATT GGTAGTGCAG   301 TTTCGAATGG ACAAAAGGAT TTTGACACGA TTGTAGCTGT TAGACACCCT TATTCTGACG   361 AAGTAGATAG AAGTATTCGA GTGGTAAGTC CTTGTGGTAT GTGTAGGGAG TTGATTTCAG   421 ACTATGCACC AGATTGTTTT GTGTTAATAG AAATGAATGG CAAGTTAGTC AAAACTACGA   481 TTGAAGAACT CATTCCACTC AAATATACCC GAAATTAAAA GTCGACGATA AAATAAAAGA   541 TTTTATTTAG TCTCCAGAAA AAGGGGGGAA TGAAAGACCC CACCTGTAGG TTTGGCAAGC   601 TAGCTTAAGT AACGCCATTT TGCAAGGCAT GGAAAAATAC ATAACTGAGA ATAGAGAAGT   661 TCAGATCAAG GTCAGGAACA GATGGAACAG CTGAATATGG GCCAAACAGG ATATCTGTGG   721 TAAGCAGTTC CTGCCCCGGC TCANGGCCAA GAACAGATGG AACAGCTGAA TATGGGCCAA   781 ACAGGATATC TGTGGTAAGC AGTTCCTGNN NNGGCTCANG GCCNAGAACA GATGGTCNNC   841 AGATGCGGTC CAGCCCTCAN CAGTTTCTAG AGAANCNTCA GANGTTTNCN GGGNGNCCCA   901 AGGANNNNAA ATGANCNNGN GCCTTATTTG AACNNNNCNA TCANTTCGCT TCNCGCTNCN 961 GNNCGCGNGC TNNNGNNNCC NNANCTNNNN AAAAGANCNN NNNNNNNN //
Primer: CMV enh_F1 (Pr0652) Region: CMV en, 5' LTR, MMLV psi Sequence file: RDB17364_A9Lge.seq
>D06377A8_A9Lg_1_CMVenh_F1_D08_11_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNTGNNC TTATGGGNNT TTCCTACTTG GCAGNNNNNN    61 TACGTATTAG TCATCGCTAT TACCATGCTA GAGAACCATC AGATGTTTCC AGGGTGCCCC   121 AAGGACCTGA AATGACCCTG TGCCTTATTT GAACTAACCA ATCAGTTCGC TTCTCGCTTC   181 TGTTCGCGCG CTTCTGCTCC CCGAGCTCAA TAAAAGAGCC CACAACCCCT CACTCGGCGC   241 GCCAGTCCTC CGATTGACTG AGTCGCCCGG GTACCCGTGT ATCCAATAAA CCCTCTTGCA   301 GTTGCATCCG ACTTGTGGTC TCGCTGTTCC TTGGGAGGGT CTCCTCTGAG TGATTGACTA   361 CCCGTCAGCG GGGGTCTTTC ATTTGGGGGC TCGTCCGGGA TCGGGAGACC CCTGCCCAGG   421 GACCACCGAC CCACCACCGG GAGGTAAGCT GGCCAGCAAC TTATCTGTGT CTGTCCGANT   481 GTCTAGTGTC TATGACTGAT TTTATGCGCC TGCGTCNGTA CTAGTTAGCT NACTAGCTCT   541 GTATCTGGCG GANCCNNGGN GNAACTGANN ANTNNNGAAC ACCCGGNNNN NACCCTGGGA   601 NANNTCCCNN GNNNNNNNGG GNNNNNTTTT NNGNNCCNAN NNNNNTNNNA AANNCNNATN 661 NNTTNNNNNT NNTNNNNGNN CCCCCNNNNN NNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.04.06

GNP_filter3_RDBDEP_html_230405.pl