Resource data sheet
Prev.
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pMRX-bsr-ss-EGFP-FM4-TMD

Retroviral expression vector enabling a synchronized trafficking assay.

Catalog number RDB17365
Resource name pMRX-bsr-ss-EGFP-FM4-TMD
Clone info. Retroviral expression vector enabling a synchronized trafficking assay, EGFP tagged with the signal sequence from mouse calreticulin (ss) at its N-terminus and FM4-TMD at its C terminus. FM4, 4 x mouse FK506 binding protein 1a (FKBP1A/FKBP12) mutant (Phe-to-Met substitution at amino acid position 37). TMD, transmembrane domain from human low density lipoprotein receptor (LDLR). See Fig.5A of Homma, Y. et al. J. Cell Biol. 218 (6): 2035-2050, 2019.
Vector backbone pMRX-IRES-bsr (plasmid)
Selectable markers Amp^r (E. coli), Blasticidin^r (mammalian cells).
Gene/insert name human LDLR cDNA mouse Calr cDNA mouse Fkbp1a cDNA
Depositor|Developer Fukuda, Mitsunori |
Other clones in our bank

External Database
human LDLR mouse Calr mouse Fkbp1a

      Reference sequence
        
       

      Distribution information

      Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
      Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Hirano, S. et al. Autophagy 12, 312-326 (FM4), 2016); (Homma, Y. et al. J. Cell Biol., in press (doi: 10.1083/jcb.201810134), 2019)
      Additional terms and conditions:
      Regarding pMRX vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literatures for pMX and pMRX vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003; Saitoh T., J. Biol. Chem. 278 (38): 36005-36012, 2003) is requested.
      Ordering Please visit Information of Request for Distribution.[link] 
      Order form 
      [Credit Card Payment]  [Bank Transfer Payment]
      Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
      Remarks Remember that you will be working with samples containing infectious virus.
      提供案内 (日本国内) [open/close]

      提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Hirano, S. et al. Autophagy 12, 312-326 (FM4), 2016); (Homma, Y. et al. J. Cell Biol., in press (doi: 10.1083/jcb.201810134), 2019)。
      付加的提供条件:
      pMRXベクターについて:利用者は、研究成果の公表にあたって pMXならびにpMRXベクターの文献 (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003; Saitoh T., J. Biol. Chem. 278 (38): 36005-36012, 2003) の引用を必要とする。
      提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
      提供依頼書 [Word]
      提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
      備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

      Catalog # Resource name Shipping form Fee (non-profit org.)
      RDB17365 pMRX-bsr-ss-EGFP-FM4-TMD DNA solution

      Please review the QC test results indicated by check icon below as well as clone information before placing your order.

      References and tips

      Original reference

      original Homma, Y., Comprehensive knockout analysis of the Rab family GTPases in epithelial cells. J. Cell Biol. 218 (6): 2035-2050 (2019). PMID 31072826.
      original Hirano, S., Differing susceptibility to autophagic degradation of two LC3-binding proteins: SQSTM1/p62 and TBC1D25/OATL1. Autophagy 12 (2): 312-326 (2016). PMID 26902585.

      Further references such as user reports and related articles (go to bottom)

      Featured content


      QC test results

      RIKEN BRC has sequenced portions of this material for quality test.
      Please review the QC test results indicated by check icon as well as clone information before placing your order.

      Test sheet RDB17365_A9Lgp1-1.pdf check

      Nucleotide sequence of a portion of this resource (if available).

      Primer: MMLV-pol_F (Pr0352)
      Region: pol region, SS, EGFP 5'
      Sequence file: RDB17365_A9Lga.seq check
      >D06377A9_A9Lg_1_MMLV-pol_F_A09_03_ABI24.ab1
          1 NNNNNNNNNN NNNNNNNGGN ATCGCAGCTT GGNNACNCGC CGCCCACGTG AAGGCTGCCG
         61 ACCCCGGGGG TGGACCATCC TCTAGACTGC CGGATCTGCC ACCATGCTCC TTTCGGTGCC
        121 GCTCCTGCTT GGCCTCCTCG GCCTGGCCGC CGCAAGATCC ATGGTGAGCA AGGGCGAGGA
        181 GCTGTTCACC GGGGTGGTGC CCATCCTGGT CGAGCTGGAC GGCGACGTAA ACGGCCACAA
        241 GTTCAGCGTG TCCGGCGAGG GCGAGGGCGA TGCCACCTAC GGCAAGCTGA CCCTGAAGTT
        301 CATCTGCACC ACCGGCAAGC TGCCCGTGCC CTGGCCCACC CTCGTGACCA CCCTGACCTA
        361 CGGCGTGCAG TGCTTCAGCC GCTACCCCGA CCACATGAAG CAGCACGACT TCTTCAAGTC
        421 CGCCATGCCC GAAGGCTACG TCCAGGAGCG CACCATCTTC TTCAAGGACG ACGGCAACTA
        481 CAAGACCCGC GCCGAGGTGA AGTTCGAGGG CGACACCCTG GTGAACCGCA TCGAGCTGAA
        541 GGGCATCGAC TTCAAGGAGG ACGGCAACAT CCTGGGGCAC AAGCTGGAGT ACAACTACAA
        601 CAGCCACAAC GTCTATATCA TGGCCGACAA GCAGAAGAAC GGCATCAAGG TGAACTTCAA
        661 GATCCGCCAC AACATCGAGG ACGGCAGCGT GCAGCTCGCC GACCACTACC AGCAGAACAC
        721 CCCCATCGGC GACGGCCCCG TGCTGCTGCC CGACAACCAC TACCTGANCA CCCAGTCCGC
        781 CCTGNNCAAA GANCCCAACG AGAAGCGCGA TCACATGGTC CTGCTGGANT TCGTGACCGC
        841 CGCCGGGNTC ACTCTCGGCA TGGACGANCT GTACAAGGGN TCCGGANNGC ANGTGGAGNN
        901 CATCTCTNCN GNANNNNGGC GCANNTTNCC NANGCGCGGN CANANCNGCN NNGNNNCANT
      961 ACNNNGGNNN NNNNNNN
      //
      Primer: EGFP-C2 (Pr0402)
      Region: EGFP 3', FM(1), FM(2), FM(3)
      Sequence file: RDB17365_A9Lgb.seq check
      >D06377A9_A9Lg_1_EGFP-C2_F09_18_ABI24.ab1
          1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN GGTCCTGCTG GAGTTCGTGN NCGCCGCCGG
         61 GATCACTCTC GGCATGGNNN AGCTGTACAA GGGATCCGGA GTGCAGGTGG AGACCATCTC
        121 TCCTGGAGAC GGGCGCACCT TCCCAAAGCG CGGCCAGACC TGCGTGGTGC ACTACACGGG
        181 GATGCTTGAA GATGGAAAGA AAATGGATTC CTCTCGGGAC AGAAACAAGC CTTTTAAGTT
        241 TACACTAGGC AAGCAGGAGG TGATCCGAGG CTGGGAGGAA GGGGTAGCCC AGATGAGTGT
        301 GGGTCAGAGA GCCAAACTGA TAATCTCCTC AGACTATGCC TATGGAGCCA CCGGGCACCC
        361 AGGCATCATC CCACCACATG CCACTCTTGT TTTTGATGTG GAGCTTCTAA AACTGGAAGG
        421 AAGATCCGGA GTGCAGGTGG AGACCATCTC TCCTGGAGAC GGGCGCACCT TCCCAAAGCG
        481 CGGCCAGACC TGCGTGGTGC ACTACACGGG GATGCTTGAA GATGGAAAGA AAATGGATTC
        541 CTCTCGGGAC AGAAACAAGC CTTTTAAGTT TACACTAGGC AAGCAGGAGG TGATCCGAGG
        601 CTGGGAGGAA GGGGTAGCCC AGATGAGTGT GGGTCAGAGA GCCAAACTGA TAATCTCCTC
        661 AGACTATGCC TATGGAGCCA CCGGGCACCC AGGCATCATC CCACCACATG CCACTCTTGT
        721 TTTTGATGTG GAGCTTCTAA AACTGGAAGG AAGATCCGGA GTGCAGGTGG AGACCATCTC
        781 TCCTGGANAC GGGCGCACCT TCCCAAAGCG CGGCCAGACC TGCGTGGTGC ACTACACGGG
        841 GATGCTTGAA GANGGAAAGA AAATGGATTC CTCTCNGGNN AGAAACAAGC CTTTTANGTT
        901 TACACTNNNC AAGCANGANG TGATCCGANG CTNGGNNGNA NGGGGTANCC CNNNNGANTG
        961 TGGGTCANAN ANNCNNANCT GANANTNNNC NTCANANTAN NNNNATNGNN NCNNNNNGGN
      1021 NNCNNNGGNN NNNNNNCCNN NNNNNNNN
      //
      Primer: IRES-R (Pr0037)
      Region: TMD
      Sequence file: RDB17365_A9Lgc.seq check
      >D06377A9_A9Lg_1_IRES-R_C10_03_ABI08.ab1
          1 NNNNNNNNNN NNNNNNCNGG NNNNNNNCGT NAGGGGGGGG GGGCGNNNNN GATCCCGCTA
         61 GAGGGAAACC GTTGCTAGCT TAAGTTAGTT AACAATTGTT ACCGCGGCCG CTTAGGATCC
        121 CCATAGAAGG AAGACCCCCA GGCAAAGGAA GACGAGGAGC ACGATGGGGA GGACAATGGA
        181 CAGAGCANAT CTGGAACCAC CGGTACCACC GGAACCACCC GTCGACCCTC CTTCCAGTTT
        241 TAGAAGCTCC ACATCAAAAA CAAGAGTGGC ATGTGGTGGG ATGATGCCTG GGTGCCCGGT
        301 GGCTCCATAG GCATAGTCTG AGGAGATTAT CAGTTTGGCT CTCTGACCCA CACTCATCTG
        361 GGCTACCCCT TCCTCCCAGC CTCGGATCAC CTCCTGCTTG CCTAGTGTAA ACTTAAAAGG
        421 CTTGTTTCTG TCCCGAGAGG AATCCATTTT CTTTCCATCT TCAAGCATCC CCGTGTAGTG
        481 CACCACGCAG GTCTGGCCGC GCTTTGGGAA GGTGCGCCCG TCTCCAGGAN AGATGGTCTC
        541 CACCTGCACT CCGGATCTTC CTTCCAGTTT TAGAAGCTCC ACATCAAAAA CAAGAGTGGC
        601 ATGTGGTGGG ATGATGCCTG GGTGCCCGGT GGCTCCATAG GCATAGTCTG AGGAGATTAT
        661 CAGTTTGGCT CTCTGACCCA CANNNNNNTG GGCTACCCCT TCCTCCCAGC CTCNNATCAC
        721 CTCCTGCTTG CCTAGTGTAA ACTTAAAAGG NTTGTTTCTG TCCCGAGAGG AATCCATTTT
        781 CTTTCCATCT TCAAGCATCC CCGTGTAGTG CACCACGCAN GTCTGGNCGC GCTTTGGGAA
        841 NNNGCGCCCG TCNCCAGGAN AGANGGTCTC CNCCTGCNCT CCNNNNCTNN CNTCNNGTTT
        901 TANAANCTCC ACATCAAAAA NNANNANTGN NATGNGGNGG GNNNANGNCN GGGNGNCCGG
        961 NNGNNTNCNT NNNNNTANTC NNNNGANNNT ATNNNNTTNN NNNNNNGANC CNNNNTCNTC
      1021 TGGGNTACCC NNNNNNNCNN NCNNNNNNNN ANNNCNNNNN NNNNN
      //
      Primer: IRES-F (Pr0036)
      Region: IRES, bsr
      Sequence file: RDB17365_A9Lgd.seq check
      >D06377A9_A9Lg_1_IRES-F_C09_09_ABI24.ab1
          1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNT TNNNAAAACG TCTAGGCCCC CCGAACCNCG
         61 GGGNCGTGGT TTTCCTTTGA AAAACACGAT AATACCATGG TCATGAAAAC ATTTAACATT
        121 TCTCAACAAG ATCTAGAATT AGTAGAAGTA GCGACAGAGA AGATTACAAT GCTTTATGAG
        181 GATAATAAAC ATCATGTGGG AGCGGCAATT CGTACGAAAA CAGGAGAAAT CATTTCGGCA
        241 GTACATATTG AAGCGTATAT AGGACGAGTA ACTGTTTGTG CAGAAGCCAT TGCGATTGGT
        301 AGTGCAGTTT CGAATGGACA AAAGGATTTT GACACGATTG TAGCTGTTAG ACACCCTTAT
        361 TCTGACGAAG TAGATAGAAG TATTCGAGTG GTAAGTCCTT GTGGTATGTG TAGGGAGTTG
        421 ATTTCAGACT ATGCACCAGA TTGTTTTGTG TTAATAGAAA TGAATGGCAA GTTAGTCAAA
        481 ACTACGATTG AAGAACTCAT TCCACTCAAA TATACCCGAA ATTAAAAGTC GACGATAAAA
        541 TAAAAGATTT TATTTAGTCT CCAGAAAAAG GGGGGAATGA AAGACCCCAC CTGTAGGTTT
        601 GGCAAGCTAG CTTAAGTAAC GCCATTTTGC AAGGCATGGA AAAATACATA ACTGAGAATA
        661 NAGAAGTTCA GATCAAGGTC AGGAACAGAT GGAACAGCTG AATATGGGCC AAACAGGATA
        721 TCTGTGGTAA GCAGTTCCTG CCCCGGCTCA NGGCCAAGAA CAGATGGAAC AGCTGAATAT
        781 GGGCCNAACA GGATATCTGT GGTAAGCAGT TCCTGCNNCG GCTCNNGGCC AAGAACAGAT
        841 GGTCCCCAGA TGCGGNCNNG CCCTCANCAG TTTCTAGAGA ANCNTCNNAT GTTTCCNGGG
        901 NGCCCCANNG ANCNGAAATG ANCCTGNGCC TTATTTGAAC TAACCNATCA GTTCGCTTCT
        961 CGCTNCTGTT NNCGNNNNNN NGNNNNNNNN CNCNNNAAAA GANCCNNCNA NCNNNNNNNN
      1021 NN
      //
      Primer: IRESup_R1 (Pr0804)
      Region: TMD, FM(4), FM(3)
      Sequence file: RDB17365_A9Lge.seq check
      >D06377A9_A9Lg_1_IRESup_R1_A11_01_ABI08.ab1
          1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNCCGCGGCC GCTTAGGNNC CCCNTAGAAG
         61 GAAGACCCCC AGGCAAAGGA AGACGAGGAG CACGATGGGG AGGACAATGG ACAGAGCAGA
        121 TCTGGAACCA CCGGTACCAC CGGAACCACC CGTCGACCCT CCTTCCAGTT TTAGAAGCTC
        181 CACATCAAAA ACAAGAGTGG CATGTGGTGG GATGATGCCT GGGTGCCCGG TGGCTCCATA
        241 GGCATAGTCT GAGGAGATTA TCAGTTTGGC TCTCTGACCC ACACTCATCT GGGCTACCCC
        301 TTCCTCCCAG CCTCGGATCA CCTCCTGCTT GCCTAGTGTA AACTTAAAAG GCTTGTTTCT
        361 GTCCCGAGAG GAATCCATTT TCTTTCCATC TTCAAGCATC CCCGTGTAGT GCACCACGCA
        421 GGTCTGGCCG CGCTTTGGGA AGGTGCGCCC GTCTCCAGGA GAGATGGTCT CCACCTGCAC
        481 TCCGGATCTT CCTTCCAGTT TTAGAAGCTC CACATCAAAA ACAAGAGTGG CATGTGGTGG
        541 GATGATGCCT GGGTGCCCGG TGGCTCCATA GGCATAGTCT GAGGAGATTA TCAGTTTGGC
        601 TCTCTGACCC ACACTCATCT GGGCTACCCC TTCCTCCCAG CCTCGGATCA CCTCCTGCTT
        661 GCCTAGTGTA AACTTAAAAG GCTTGTTTCT GTCCCGAGAG GAATCCATTT TCTTTCCATC
        721 TTCAAGCATC CCCGTGTAGT GCACCACGCA GGTCTGGCCG CGCTTTGGGA AGGTGCGCCC
        781 GTCTCCAGGA NAGATGGTCT CCACCTGCAC TCCNGATCTT CCTTCCNGTT TTAGAAGCTC
        841 CACATCAAAA ACAAGAGTGN NATGTGGTGG GNNGANGCCT GGGTGCCCNG GTGGNTCCNN
        901 NNGCATAGTC TGAGGAGANT ATCNNTTTGG CTCTCTGANC CNCACTCATC NGGGCTACCC
        961 CTTCCTCCCN GCCNNGNANN ACNNNCTGCN NGNCNANNGN AAACTTAAAN NNNNNGNTTC
      1021 TGNNCCNNNA NNNANNNNNT TNCTTTNNNT NNNNNNNNNT NCNCNNNNNN NNNN
      //
      Primer: CMV enh_F1 (Pr0652)
      Region: CMV en, 5' LTR, MMLV psi
      Sequence file: RDB17365_A9Lgf.seq check
      >D06377A9_A9Lg_1_CMVenh_F1_D09_12_ABI24.ab1
          1 NNNNNNNNNN NNNNNNTGNN NNGNNNNNNN NCTTATGGGN NTTTCCTACT TGGCAGTACN
         61 NTCTACGTAT TAGTCATCGC TATTACCATG CTAGAGAACC ATCAGATGTT TCCAGGGTGC
        121 CCCAAGGACC TGAAATGACC CTGTGCCTTA TTTGAACTAA CCAATCAGTT CGCTTCTCGC
        181 TTCTGTTCGC GCGCTTCTGC TCCCCGAGCT CAATAAAAGA GCCCACAACC CCTCACTCGG
        241 CGCGCCAGTC CTCCGATTGA CTGAGTCGCC CGGGTACCCG TGTATCCAAT AAACCCTCTT
        301 GCAGTTGCAT CCGACTTGTG GTCTCGCTGT TCCTTGGGAG GGTCTCCTCT GAGTGATTGA
        361 CTACCCGTCA GCGGGGGTCT TTCATTTGGG GGCTCGTCCG GGATCGGGAG ACCCCTGCCC
        421 AGGGACCACC GACCCACCAC CGGGAGGTAA GCTGGCCAGC AACTTATCTG TGTCTGTCCG
        481 ATTGTCTAGT GTCTATGACT GATTTTATGC GCCTGCGTCG GTACTAGTTA GCTAACTAGC
        541 TCTGTATCTG GCGGACCCGT GGTGGAACTG ACGAGTTCGG AACACCCGGC CGCAACCCTG
        601 GGAGACGTCC CAGGGACTTC GGGGGCCGTT TTTGTGGCCC GACCTGAGTC CAAAAATCCC
        661 GATCGTTTTG GACTCTTTGG TGCACCCCCC TTAGAGGAGG GATATGTGGT TCTGGTAGGA
        721 GACGAGAACC TAAAACAGTT CCCGCCTCCG TCTGAATTTT TGCTTTCGGT TTGGGACCGA
        781 ANCCGCGCCG CGCGTCTTGT CTGCTGCAGC ATCGTTCTGT GTTGTCTCTG TCTGANTGTG
        841 TTTCTGTATT TGTCTGAAAA TATGGGCCCG GGCCAGANTG TTACCACTCC NNTAAGTTTG
        901 ANCTTNNNNC ANTGGAAAGA NGTCGAGCGG NTCGCTCNCN NCCNGNCNGT ANANGTCNAG
      961 NNNANNNNNT GGNNNANCNT NNNGNNNNGN NNANNNNNCN NNNCNTTNNN NNNNNNN
      //
      Primer: bsr-C_F (Pr0803)
      Region: bsr, 3' LTR
      Sequence file: RDB17365_A9Lgg.seq check
      >D06377A9_A9Lg_1_bsr-C_F_E09_15_ABI24.ab1
          1 NNNNNNNNNN NNNNNNNNGT TTTGNGNNNN NGAANNGAAT GGCAAGTTAG TCAAAACTAC
         61 GATTGAAGAA NNCNTTCCAC TCAAATATAC CCGAAATTAA AAGTCGACGA TAAAATAAAA
        121 GATTTTATTT AGTCTCCAGA AAAAGGGGGG AATGAAAGAC CCCACCTGTA GGTTTGGCAA
        181 GCTAGCTTAA GTAACGCCAT TTTGCAAGGC ATGGAAAAAT ACATAACTGA GAATAGAGAA
        241 GTTCAGATCA AGGTCAGGAA CAGATGGAAC AGCTGAATAT GGGCCAAACA GGATATCTGT
        301 GGTAAGCAGT TCCTGCCCCG GCTCAGGGCC AAGAACAGAT GGAACAGCTG AATATGGGCC
        361 AAACAGGATA TCTGTGGTAA GCAGTTCCTG CCCCGGCTCA GGGCCAAGAA CAGATGGTCC
        421 CCAGATGCGG TCCAGCCCTC AGCAGTTTCT AGAGAACCAT CAGATGTTTC CAGGGTGCCC
        481 CAAGGACCTG AAATGACCCT GTGCCTTATT TGAACTAACC AATCAGTTCG CTTCTCGCTT
        541 CTGTTCGCGC GCTTCTGCTC CCCGAGCTCA ATAAAAGAGC CCACAACCCC TCACTCGGGG
        601 CGCCAGTCCT CCGATTGACT GAGTCGCCCG GGTACCCGTG TATCCAATAA ACCCTCTTGC
        661 AGTTGCATCC GACTTGTGGT CTCGCTGTTC CTTGGGAGGG TCTCCTCTGA GTGATTGACT
        721 ACCCGTCAGC GGGGGTCTTT CACATGCAGC ATGTATCAAA ATTAATTTGG TTTTTTTTCT
        781 TAAGTATTTA CATTAAATGG CCATAGTTGC ATTAATGAAT CGGCCAACGC GCGGGNNNAN
        841 GCGGTTTGCG TATTGGGCGC TCTTCCGCTT CCNCGCTCAC TGANTCGCTG CGCTCGGTCG
        901 TTCGGCTGCG GCGAGCGGTA TCAGCTCACT CAANNGNGGN AATACNGNNA TCCNCAGAAT
        961 CNGGGNNNAA CNCNNNNAAN NNNNNGNGNN CAAANNNNCA NNNNANNNNN NNGNNNNCNN
      1021 NNAAANNNNC NNNNNNNNNN
      //

      Please visit Sequencing and PCR primers for primer information.


      References

      Original, user report and related articles

      original Homma, Y., Comprehensive knockout analysis of the Rab family GTPases in epithelial cells. J. Cell Biol. 218 (6): 2035-2050 (2019). PMID 31072826.
      original Hirano, S., Differing susceptibility to autophagic degradation of two LC3-binding proteins: SQSTM1/p62 and TBC1D25/OATL1. Autophagy 12 (2): 312-326 (2016). PMID 26902585.

      2023.04.06

      GNP_filter3_RDBDEP_html_230405.pl