Please review the QC test results indicated by icon below as well as clone information before placing your order.

pCAG_mRsph10b-PA

Expression vector of mouse Rsph10b.

Sequence (full)	RDB17452zzk02.seq provided by the depositor

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested.(Abbasi, F et al. J. Cell Sci. 131(19) jcs221648, 2018)
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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Abbasi, F et al. J. Cell Sci. 131(19) jcs221648, 2018)。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

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Materials & Methods section:

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Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB17452_A9G9p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1 (Pr0164) Region: insert 5' Sequence file: RDB17452_A9G9a.seq
>D06337A4_A9Gg_2_pAxCA_F1_E02_14_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNGC CTTCTTCTTT TTNNNNCNGC TCCTGGGCAA CGTGCTGGTT    61 ATTGTGCTGT CTCATCATTT TGGCAAATCT AGAGCCGCCA TGGTGAAAGA AAAGAAAAAA   121 GCGGACAAGA AAGGAGATAA GTCAGCCCGC TCTCCCTCCT CCATTTCTGA TAACCCAGAA   181 GCCTCGAAAC AAGATAGCAA TGCCAGTAAG CAAGAAGTGG CCCCCAGTGC TGTTGTCCCA   241 GTCGTGGAAA CACCACTCAA GCAAGCCCCG AAAAGAGACT CAGTACAAAT GGAGCAGAGT   301 GAAGAGGAGA CTCAGTACGA AGAGCCCATC CTGACCAAGC TCATAGTGGA AAGCTATGAA   361 GGGGAGAAAG TCCGTGGACT GTATGAGGGA GAAGGCTTTG CTGTCTTTCA AGGCGGAAAC   421 ACCTACCACG GTATGTTTTC AGAAGGACTG ATGCATGGAC AAGGCACTTA CATTTGGGCT   481 GACGGATTGA AGTACGAGGG GGACTTTGTG AAGAATATCC CCATGAACCA CGGGGTGTAC   541 ACATGGCCGG ACGGCAGCAC TTATGAAGGA GAGGTGACCA ATGGCATGAG GAATGGATTT   601 GGTATGTTCA AGTGTGGCAC ACAGCCTGTG TCCTACATCG GCCACTGGTG TCATGGCAAG   661 AGACATGGGA AGGGCTCCAT CTATTACAAT CAAGAGGGGA NCTCTTGGTA TGAAGGAGAC   721 TGGGTATACA ACATCAAAAA GGGCTGGGGA ATAAGANGCT ATAAATCANG AAACATATAT   781 GAAGGGCAGT GGGAGAACAA CATGCGCCAT GGGGAANGCA GAATGANGTG GCTCACNACA   841 AACGAGGAGT ACACGGGCCA CTGGGGANAA NGGGANCCAG ANNGGCTTTG GAACNCACAC   901 NTGGTTCCTA AAGAGAATCC CCAANTNTCA NTATCCCTTG AGAAANGAAT NNNTANGGGN 961 ANTNNNAAAT GGNTNNCGNC NNGNNNNNNG NNAATTTTNN TACNNNNNNN NNN //
Primer: pCA-bgter (Pr0066) Region: PA tag, insert 3' Sequence file: RDB17452_A9G9b.seq
>D06337A4_A9Gg_2_pCA-bgter_F02_17_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNTT NNNTGNNNGA NGNNNNNNNN CTCNGTGGTA TTTGTGAGCC    61 AGGGCATTGG CCACACCAGC CNCCACCTTC TGATAGGCAG CCTGCACCTG AGGAGTCTCG   121 ATTTACACCA CATCATCTTC GGCACCTGGC ATGGCAACGC CGCTAGCCTC GAGGGCCGCG   181 TCGACAAGCT TCTTCTTCTT CTTCTTGCCA GGGCTGGCCT TGCTAGTCTG GATGGAAGCC   241 AGGTCCTCTT TAGGGGGGCT GGGCGTGAGA GGCTGAGAGG CTGGGATCTC CGATGGCTCC   301 TTGAGCACAG TGATGTCAAG TTCGAAGTCT TGTTTCCTGG CCTCCTCTTC CCGTAGGATG   361 TTCAGCCTGG CTTCTAGCTC TTCGTTCTCT ATCTGCCTCT GCTGAGCCAG CTCTGCTTCC   421 TGGAGCTGAT TTTCCTTCAC TTTCTCCTTG AGCATCTCTT CACGTTTGTA GGCACTGAAG   481 AGCACACTTA CAAAGAAGAC ATAGAGGTTG TTCACCCATA TGTTGAGTTT CTCTTTCTGT   541 TCATCTTTGG GCTTTTCATA TTTCTCCTGT GGAGACATAA ACACCAGGCC TCTTCCTTGA   601 GTCTTGAAGT TCATTTTGGA GACTTTGTCC TCACTCAGCG ACTTCTTGAG CTTGGGCTCT   661 GACTGCCTGA TTTTACCATC AGGCAGAGCG CCTATCTTGT CTAAGGACGA CTTGGTGCTG   721 CTGAAGTGGA CTTCGGAGTC ATGGCTTGTG ATTGCACTCG GGCTCCTGTG GANGCTCTGG   781 TTCAGTATCG TCTGANTGCT GCCGTATCGG TTTGTAGTGA TGTCGTCATA CGGGNNTTTT   841 AGATAGGNTC TAGTCATCTG GTCAAACATG CAGCAGANNN ANAANCATAA NANGNNNNNN   901 AANANNTNNA GGAAANNCAG CTCAAGCTCG AANTTGCTGT CNNTTNNNTC NNNNNCNAAA   961 GGGNTGTCNN NCNNNNTCAC ANNCATGAAN TTNGNNNNNA NNTNNNNNNN TNNNNNNNNN 1021 N //
Primer: pUC-CAPbs_up_F (Pr0536) Region: lac pro, lacO, T3 pro, CMV en, CAG pro Sequence file: RDB17452_A9G9c.seq
>D06337A4_A9Gg_2_pUC-CAPbs_up_F_G02_20_ABI24.ab1     1 NNNNNNNNNN NGNNNNNNNC NCNNCGCNNT AANNTGAGTT AGCTCACTCA TTAGGCACCC    61 CAGGCTTTAN NNNNNNNNCT TCCGGCTCGT ATGTTGTGTG GAATTGTGAG CGGATAACAA   121 TTTCACACAG GAAACAGCTA TGACCATGAT TACGCCAAGC GCGCAATTAA CCCTCACTAA   181 AGGGAACAAA AGCTGGAGCT CATCGATACT CGACATTGAT TATTGACTAG TTATTAATAG   241 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT   301 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG   361 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT   421 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT   481 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG   541 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTCGAGGTG   601 AGCCCCACGT TCTGCTTCAC TCTCCCCATC TCCCCCCCCT CCCCACCCCC AATTTTGTAT 661 TTATTTATTT TTTAATTATT TTGTGCAGCG ATGGGGGCNN NNGGGGGGNN NNNNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.05.01

GNP_filter3_RDBDEP_html_230501.pl